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teslacann
Strange


Registered: 11/10/20
Posts: 76
Last seen: 2 years, 10 months
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I think I'm getting there?
#27104418 - 12/23/20 03:45 PM (3 years, 1 month ago) |
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Hello everyone, I've been at this for 3 months and so far no luck. BUT! I believe I'm finally getting somewhere!
I wish I'd asked sooner before attempting to do transfer and the like but I was too impatient!
This is my small attempt at transferring an agar slice to grains

It looks like there is a white "fluffiness" sitting on top of what I think is actual mycellium?
These are my plates
 I know there is some bacterial stuff going on there, but, the main thing im wondering about is that fluffy whiteness before the obvious mycellium growth out the sides. Is that tomatose growth or mold?
 This plate here makes me think it's mold. You can see there are 2 patches of white fluffiness not connected to the transfer. Again, I'm just not sure.
These 2 plates here are a transfer of the same culture. To me they look kind of weird.

 To be fair though, this was using the MEA recipe I originally found which was 3g/3g to 250ml of water. I've now found out that it's actually meant to be about double that? Could that be the reason it looks so weird? Thanks in advance for your help! Teslacann
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Forrester
aspiring sociopath


Registered: 02/05/13
Posts: 9,351
Loc: Northeast USA
Last seen: 1 month, 12 days
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Re: I think I'm getting there? [Re: teslacann] 1
#27104440 - 12/23/20 03:57 PM (3 years, 1 month ago) |
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That's what she said.
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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teslacann
Strange


Registered: 11/10/20
Posts: 76
Last seen: 2 years, 10 months
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Re: I think I'm getting there? [Re: Forrester]
#27104501 - 12/23/20 04:40 PM (3 years, 1 month ago) |
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Does that mean yes? Or as usual.. no?
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bw86
Doesn't play well with others


Registered: 11/12/06
Posts: 5,941
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Re: I think I'm getting there? [Re: teslacann] 1
#27104519 - 12/23/20 04:46 PM (3 years, 1 month ago) |
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That pic is too blurry to make out whats on top. What kind of container is your grain in? with what lid?
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teslacann
Strange


Registered: 11/10/20
Posts: 76
Last seen: 2 years, 10 months
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Re: I think I'm getting there? [Re: bw86]
#27104528 - 12/23/20 04:53 PM (3 years, 1 month ago) |
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i've tried to take better ones but from them all that's honestly the best I can get it to focus.
That is a SATCO 200ml PP5 tub. I was using them originally for patsy plates but I reckoned they'd probably do the job as jars too. Mainly because I didn't actually get jars until today but I wanted to crack on. I melted a hole in the side and parafilmed it, the idea being it would work similiar to a filter and allow gas exchange without other nasties getting in.
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teslacann
Strange


Registered: 11/10/20
Posts: 76
Last seen: 2 years, 10 months
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Re: I think I'm getting there? [Re: teslacann]
#27106362 - 12/24/20 06:58 PM (3 years, 1 month ago) |
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I have binned all of this attempt. I became convinced the white fluffiness was not good.
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