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 GypsyBastard
Misanthrope
Registered: 11/30/20
Posts: 846
Loc: The Mighty Boosh
Last seen: 3 years, 1 month
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 LAGM2021- Official Let's All Grow Mushrooms 2021   5
#27103145 - 12/22/20 09:28 PM (3 years, 3 months ago) |
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----In the honor and absence of CBK, I am starting an official thread to serve as both the sign up and the main thread. Will try to keep format identical to last years.-----Start Date: Friday January 1st 2021
After a long and turmoilous year I think we all could use a good common cause to gather around and recharge as well as learn and grow together in more ways than one. "Okay let's get this going again!! We had so much fun last time. This really brought the community together back in 2017(and 2020) and I cant think of a better time to get a community grow happening than 202(1)!" If you're new to agar dont be shy! Sign up! Grab your supplies! Plenty of time before the new year!" Okay... A few rules! -All species and varieties welcome! -We will be starting from spores!! -ALL SPORES HAVE TO GO ON AGAR FIRST! -We start January 1st spores to agar -Add as much info as you can. This is a great learning tool for us and other users to follow along. "Mistakes" and all! -Post your updates in the thread as well as updating your OP to keep the conversation going - Have fun! Most importantly  To Sign up Comment in this thread to reserve your spot. This comment will be your grow log. Keep updating it as we go along. I will link to your spot in the OP below Let me know the species and variety you plan on growing for the log! Sign up closes on Jan 1st 2021 I'll have a mod move this thread to mush cult on the 1st Let's see how many people we can get on board this year!  Communal grow participants: A.k.a.Species: P. Cubensis Variant: Argentinian, PESH Species: P. Tampanensis Variant: Alt7 Species: Pan. Nec-D AbabyphoenixSpecies: P. Cubensis Variant: MM, RW x MM F2, APEU adhocSpecies: P. Cubensis Variant: Dancing Tiger AdrenochromeSpecies: P. Cubensis Variant: PESA alaskappalachianSpecies: P.Cubensis Variant: Golden Halo, AA+ Species: Ps. Caerulescens Species: P. Semilanceata allthesporesSpecies: Pan. Cyanescens Variant: Florida AmbroseSpecies: P. Mexicana Variant: Jalisco, Chico Nindo anatomalitySpecies: P. Cyanescens Species: P. Tampanensis Species: P. Cubensis Variant: APE, PEU, Creeper, Huautla, Daddy Long Legs BallzagnaSpecies: P. Cubensis Variant: LPEU, APE, Yeti Species: Pan. Cyanescens Variant: FSU bigfootscreepyunclSpecies: P. Cubensis Variant: PE, APE, GT BuckarooSpecies: P. Cubensis Variant: AA+ BuddakingSpecies: P. Cubensis Variant: PE Camera93Species: P. Cubensis Variant: Oak Ridge capntripsSpecies: P. Cubensis Variant: B+, AA+, GT Species: P. Tampanensis cronicrSpecies: P. Cubensis Variant: PE, tat, gandalf coversallSpecies: P. Cubensis Variant: Unknown Species: P. Galindoi Variant: Unknown CrakatoaSpecies: P. Cubensis Variant: RW X MM, AA+ "PMG", Natalensis and Nichrome's Playa Uvita cross D3monicSpecies: P. Cubensis Variant: x7x(f2), Melmac Doctor MarioSpecies: P. Cubensis Variant: Avery's Albino, Yeti, Rusty Whyte, Whyte Gold, TOC, APEU, PESA DrunkUncleSpecies: P. Cubensis Variant: APE, Melmac (TOC) falconmanSpecies: P. Cubensis Variant: B+ filthykneesSpecies: P. Cubensis Variant: PESA/PE FrostyStipeSpecies: P. Cubensis Variant: APE FungusFreedomSpecies: P. Cubensis Variant: CRS Species: Pan. Cyanescens Variant: Peace River GastronomicusSpecies: P. Cubensis Variant: Amazonian, RustyWhyte gdphanboomersSpecies: P. Cubensis Variant: GT, Blue Meanie Genghis ChronSpecies: P. Cubensis Variant: Melmac, AA+ GOOMY840Species: P. Cubensis Variant: Mckennaii, Jedi Mind Fuck, Maz GrinchGrowerSpecies: P. Cubensis Variant: Escondido GringoCazadarSpecies: TBD GypsyBastardSpecies: P. Cubensis Variant: GT, PFC, Z, AA+, PR, CR HappinessStanSpecies: P. Cyanescens hantyumiSpecies: P. Cubensis Variant: KSSS, B+ HaywireSpecies: P. Cubensis Variant: Texas Orange Cap IambrownbearSpecies: P. Cubensis Variant: RustyWhyte, Nepali Chitwan IndulgenceSpecies: P. cubensis Variant: Pearly gates, Tat Species: P. Allenii InthepitSpecies: P. Cubensis Variant: APE ironcapSpecies: P. Cubensis Variant: PF Redspore Jarhead3521Species: P. Cubensis Variant: Huatla, Malabar Coast jay.achSpecies: P. Cubensis Variant: B+ JosexSpecies: P. Cubensis Variant: PE junk_f00dSpecies: P. Cubensis Variant: I forgot Just_A_NoobSpecies: Pan. Cyanescens Variant: Goliath, Hawaii KeefersuntherlandSpecies: P. Cubensis Variant: GT, LPEU KromazoneSpecies: P. Cubensis Variant: APE LeafyClarkSpecies: P. Cubensis Variant: Ecuadorian, Lizard King LemonTeknoSpecies: P. Cubensis Variant: GT lookintolearnSpecies: P. Cubensis Variant: Argentinian, PE, KSSS, TOC Madscience039Species: P. Cubensis Variant: Pearly Gates, Black Caps MelliferousSpecies: P. Cubensis Variant: RustyWhyte, PE, Whytegold, Melmak, Ghost, TAT, Gandalf MH5109Species: P. Cubensis Variant: AA+ mind.at.largeSpecies: Pan. Cyanescens Species: P. Cubensis Variant: Melmac, TWC Mr. MushieSpecies: P. Cubensis Variant: RustyWhyte mushboySpecies: P. Cubensis Variant: AA+ (CBK) mushheadSpecies: P. Cubencsis Variant: Burma Species: Pan. Cinctulus MuthaFudpuckerSpecies: P. Cubensis Variant: PE Species: P. Tampanensis Variant: Pollock Myc_HuntSpecies: P. Cubensis Variant: PESA Species: P. Natalensis Species: Pan. Cyanescens Variant: Wild Coast Species: P. Tampanensis Species: Pleurotus citirinopileatus MycanthropeSpecies: P. Cubensis Variant: B+ MycoBrainzSpecies: TBD NarratorSpecies: Pholiota Adiposa Species: P. Cubensis Variant: APE, Mystery Mousekatool NichromeSpecies: P. Cubensis Variant: Fiji Monster Species: P. Caerulescens Variant: Field collected Species: G. Luteofolius Variant: BC nogoodnamesleftSpecies: P. Cubensis Variant: White Gold, PESA One of UsSpecies: P. Cubensis Variant: TAM, Pearly Gates Species: Pan. Unknown Variant: South African Species: P. Cyanescens Species: P. Caerulipes PitcherCrabSpecies: P. Cubensis Variant: TOC, TWC PuduwokeSpecies: P. Cubensis Variant: APE, Melmac Species: Pan. Cyanescens Variant: Red down under pureshroomingSpecies: P. Cubensis Variant: B+ QlockworkSpecies: P. Cubensis Variant: Pearly Gates Quirkmeister92Species: P. Cubensis Variant: RustyWhyte Species: P. Tampanensis Species: P. Hoogshagenii Variant: Convexa Specie: Pan. Cinctilus Variant: Warrens redbeeSpecies: P. Cubensis Variant: APEU roarkellSpecies: G. Lutefolius Species: P. Cubensis Variant: "randos" RoscoeReturnsSpecies: P. Cubensis Variant: Ape, Melmac seldom seenSpecies: P. Cubensis Variant: Huatla, Pink Buffalo Species: Pan. Bisporus Species: P. Tampanensis SockadinSpecies: Cubensis Variant: PA+ Species: Pleurotus Eryngii Sold Out OnlineSpecies: P. Cubensis Variant: PE6, RustyWhyte, APE, PESH Spore-hunterSpecies: P. Cubensis Variant: Pearly Gates squishy_zorroSpecies: P. Cubensis Variant: Melmac, PESH starbonesSpecies: P. Cubensis Variant: PEU, PE, Melmac SvetaketuSpecies: P. Cubensis Variant: RustyWhyte, Leucistic Cambodian Tankie_JSpecies: Pan. Cyanescens Species: P. Cubensis Variant: Maz, Blue meanie, attempt to cross Melmak\APE, Whytegold Species:P. Baeocystis The DalcassianSpecies: P. Cubensis Variant: NST Tight LunchboxSpecies: P. Cubensis Variant: Nepal Chitwan TattersailSpecies: P. Cubensis Variant: KSSS Species: G. sp. TigerStrikeSpecies: A. Bisporus tiptrippySpecies: P. Cubensis Variant: x7x Species: Pan. Cyanescens trypkaloidsSpecies: P. Cubensis Variant: Envyss, APE, RustyWhyte TweeqSpecies: P. Cubensis Variant: PESA, GT VinciSpecies: P. Cubensis Variant: APE WizardSJSpecies: P. Cubensis Variant: Maz WonkoSpecies: P. Cubensis Variant: Brazilian
Edited by GypsyBastard (02/25/21 05:20 PM)
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GypsyBastard
Misanthrope
Registered: 11/30/20
Posts: 846
Loc: The Mighty Boosh
Last seen: 3 years, 1 month
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 Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: GypsyBastard] 1
#27103148 - 12/22/20 09:29 PM (3 years, 3 months ago) |
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   GypsyBastard presents: LAGM2021 Grow Log Species:
 P. Cubensis
Variant:
 GT
PFC
Z
AA+
PR
CRJust poured my plates. I'm a bit rusty and shaky in my sterile tek but it'll come back to me soon enough. Couldn't find my media bottles so used a rando glass bottle with a pp5 lid but it was a bit bigger than I'm used to at almost 1L; took longer to remelt and seemed harder to find that sweet spot of liquid yet palatable to the fingertips. As a result some of the plate are gonna be a bit lumpy and overfilled. Normally I would allow plates to cool for 24 hours in SAB before wrapping but I may need to move the SAB between now and next year so I will likely wrap them only an hour or two over cooling. I expect to see this bite me in the ass later but it is what it is....Equipment and Materials
Agar- 10g agar, 10g Potato Starch, 7g Dextrose
Plates- 100mm x 15mm
SAB- 34" x 18" x 12"
Wrap- 2" wide cheap plastic wrap
January 2nd, 2021While fruitlessly looking for sterile swabs, some old prints and swabs were found. As such, a few variants have been added. A CRS print was also found but did not appear to have any usable spores on it; to the point that it could not be discerned even where the print used to be on the foil. In total 11 plates were inoculated; some by loop, some be swab, some by MSS.
The Breakdown:
- 2 plates AA+; via loop
-2 plates GT; via loop
-2 plates Z; via loop
-2 plates PFC; via MSS
-2 plates PR; via swab
-1 plate CR; via swab
Now we wait.... January 4th, 2021Ok, quick update: A Haiku
No germination
Except some bacteria
Guess I'll wait longerJanuary 6th, 2021We Have Germination! (the good kind)The Breakdown:
1 PR plate
1 GT plate The PR is a surpise. It is from a swab made from an MSS about a year ago. It was funky as fuck in growth and from what I've read/heard it's still pretty unstable so I happy to be able to play around with it again. GT is a favorite. One of my first and most worked with. This is sort of my workhorse variant and can't wait to see what it puts out this time around. No visible myc growth on the other plates yet though I expect to see in the next 24 hours or so. Seeing growth on the PR plate gives me hope for the CR plate which comes from a swab made in the same fashion at the same time. Fully expect other GT plate to be very close behind and Z to catch up and perhaps over take in terms of speed. AA+ is showing bacterial contams so I'll let thos ride it out and clean them up later. This is also expected due to my own ignorance when the print was made. The PFC plate was noc'd using via MSS and the excess moitsure is likely to further delay germination though I do expect it to happen. Moving cakes to HC today which were noc'd via the same MSS.
Current growth is 50%-70% the area of a dime so I will hopefully have time and motivation to make T1s tomorrow. T0 plates will be one time use but T1-T2 will be done on the same plate. T3 will be done on its own plate as it will go to grain.
Excited to see signs I still kinda know what I'm doing!  January 8th, 2021getting pretty bummed that I lost my LME while sitting here staring at PDA plates that (in my mind based off past experience with these) would have germd by now MEA. Reached to an old friend that would know to find out where to get some malt extract around here. (just moved) Bad news is not a single place in town. Good news is he has a pound of it I can grab on Monday.
So unless I just get antsy and can't wait, I'll make up some more (better) agar on Monday and make T1s and probably some more germ plates for anything now going by then.
I've never had plate NOT germinate. Ever. From the first time I started agar. Plenty of dirty plates, but never just no growth. The AA+ has some bacteria but I knew it was a dirty print so no surprise there. While a couple of the others are a bit difficult to see due to condensation, I see no signs of contams in them yet and I can see spores. They are just holdin' out on me.January 9th, 2021Two steps forward, one step back.
The Z plate that had germinated now appears to be mold. However, the GT and PR plates continue to look good, despite being quite light and wispy (as good as they can on this PDA). On the upside, It looks like I have germination in both PFC plates. These were noc'd via MSS so kinda surprised they were not first to germ. Still very happy to see any favorable life in these guys right now. January 14th, 2021Made a couple T1s and started more T0s. Mostly just wanted to get everything on MEA instead of PDA. A bit of ups and downs so I'll try to detail them separately. T1
-GT
-AA+
T0
-Z
-CR
-PFC
-AA+The Bad: Working in less than ideal settings; lighting was terrible and myc was quite hard to see while in SAB. Took 2-3 wedges out of T0s for T1s but I am not 100% sure I actually got any myc. The Good: MEA plate came out looking great! Like looking through amber. Poured a full sleeve but used less than half so plates ready to go for next transfers. Of the PDA plates I pour and did not use, none of them are showing any contamination. I'll not likely use them for anything unless no other option but it is a nice reassurance that my technique hasn't completely gone to shit. January 17th, 2021Finally getting some action. Not much to report on most but GT and AA+ T1s are looking great. GT is my workhorse and this print came from like 10th generation of my work with it. AA+ I only worked with shortly before just kinda letting it go and forgetting about it so excited to see those come back. GT
  
AA+
  Mini Update
AgarWhile my plates are not like crystal, I really have no need to get them more clear than they are. While the mix and procedure is fairly customized to fit my needs and preferences I feel it's a pretty easy and straightforward way to get your plates within the top 5 percentile of clearest plates. Telephone agar-7.5g
LME-15g
tap water-450ml- Pour 450ml of water into glass beaker.
- Add stir bar, sit on mag stirrer. Turn on stir bar and heat.
- Add LME to beaker -I don't dump it all a once but also not super slow. Maybe 10-15 seconds total time. Dumping it all at once would probably be fine, this is just a habit I developed when working with Potato Starch for PDA.
- Add agar to beaker
- Place thermometer in holder continue stirring/heating until a minimum of 185F is reached -I usually got to 200F but haven't gotten away with pulling at 185-186 without issue.
- Pour into media bottles and PC
To serve a bit of a dual purpose, here's a pic of my GT T1 with some kickass growth on it. Of 20 plate sleeves, usually no more than 2 will have a bit of sediment like the one shown. I'm not too worried about10% having pieces like that, it's the cloudiness I hate.   If you check back to my update on the 17th you can see how much that GT has grown in less than 24 hours and I am stoked about that. I may go ahead and start taking T2s today. AA+ is looking very similar. Z still seems to be on the struggle bus but my have germination on the new PFC T0. January 19th, 2021Progress at last!! Here's the Breakdown: T2
AA+ - 2 wedges each from 3 T1 growths
GT - 2 wedges each from 2 T1 growthsT1
PR - R&R:pin mold; poke
Z - R&R"pin mold; 2 wedges from T0
PFC(a) - 2 wedges taken from 0102 T0
PFC(b) - 2 wedges taken from 0114 T0 CR is still holding out on me. Thinking my swabs are just shit; still hold out some hope though. PFC(a) and PFC(b) germinated about the same time despite a 12 day different. I think this is due to (a) being on PDA and (b) being on MEA. Naturally, I took Ts from both. Both was noc'd with the same MSS made by me from a print that was also made by me. Z and PR are going to be a challenge. The T1s are Rescue and Recovery plates. There is some cube growth intertwined with some pin mold but I think I may be able to save them. Tried a somewhat modified poke method using tweezer for one, and tried to catch the outer most growth on wedges with the other. Time will tell. AA+ and GT are doing well it seems and already jumping off to the new plate. Very excited to have AA+ back after a couple years of it falling through the cracks. GT is my work horse and while I know starting over from a print can leave a lot of wiggle room for performance, I am cautiously optimistic that they will not disappoint. Also pictured but not part of LAGM are some PFC clones. These are some pins I pull off a recent BRF cake when birthed. They are the first fruits (FF) I have harvested in over a year. However, they were NOT handled properly. Exposed to open air, I picked them barehanded, and they sit in a square ziplock container for a few days. I did not even bother splitting them, though I did at least transfer them using some flamed tweezers. I expect lots of bacteria in the near future. I figured it was my best chance at building up my cultures and spawn quickly. 4 pins on 3 plates and 1 plate attempting a tweezer poke again. We'll see what happens.
    R&R plates:   January 26th, 2021  Got a little impatient and my AA+ T1 was looking good so I took a chance. Put about 1\3 of plate into each of 3 pt jars of WBS. I got lazy though and didn't rinse grain well enough resulting in some lumps that refuse to break up. We'll see what happens. About to pour some more plates and make some more transfers. Was checking out some plate with my scope and while I am in no way trained to ID contams, there are some plates and sectors I've decided look especially good and am going to pick up the pace a bit. Basically, every plate I transfer from I'll let grow out and if it looks good it goes to grain. Meanwhile I'll be transferring best looking\fastest growth more quickly than before. This should give me more fruits sooner and better more fruits later. Finished making transfers around 7 or 8. Made the first few T3s and more T2s. I think only 1 T1 this round. Have been checking out some of the T0s that didn't germ with my scope. Spores look healthy as far as I can tell and see no signs of germination. These are relatively old spores and were not stored properly for part of that time but I will just keep waiting. Hopefully something will pop off eventually. Not sure WTF is going on with the CR T0. Got some growth that looked odd but hasn't changed color or put out obvious pin mold or bacterial growth. Just looks like unorganized growth under the scope (I am not a train mycologist. It's possible I am simply missing the identifying features). When slicing for transfer though my scalpel hit something in the agar. Plate was noc'd with a swab and growth is from area where I dug swab in so possibly a piece of the swab? Put it on a slide but can't tell for sure what the hell it is. Went ahead with transfer and we'll see how it plays out. All in all, a very productive day today despite some very dumb and avoidable setbacks early in the day. February 25th, 2021 Forgot to get pics before shaking. Will try to be better about getting pics are the grow progresses. Started today: 5 shoeboxes (AA+ & PFC) 1 mini(PFC) Started previously: 1 shoebox (AA+) Not sure where I went wrong.... I know I started more than 2 variants but somehow along the way I seem to have forgotten about some and am now only working with AA+ and PF Classic There is still a RustyWhyte germ plate to working and some z plates I can transfer from. Not sure what the hell went wrong with my GT. It was my go-to for years but having just terrible growth and contam issues with my print this time around. May just dig out a fresh print and try again. They hold a nostalgic place in my heart and don't want to lose that line, but LAGM has highlighted some limitations I have with time and work space so I may wait a bit to restart those. Just the 6 shoeboxes, 1 mini, and a handful of cakes is proving to be more of a hassle than I would like currently and would rather grow a few tubs well, rather than a bunch poorly. Each shoeboxes have roughly 2qt coir and varying amounts of spawn. Some are 1:4, some 1:2. Mini holds about 3qt coir (one of the first I ever made and holes are a bit lower than they should be so it's basically just a tall shoebox) and has a spawn ratio of around 2:3. The one shoebox started previously is about a week along (will check dates later) and is looking pretty close to pinning. My confidence in the cleanliness of the spawn is not as high as I would like. It was the 1 jar of three that didn't not show obvious signs of contams. However, all three jars were noc'd with the same plate. I gave it an extra shake and plenty of time for them to show up and did not see any deal breakers but still know that it is less than ideal. This is actually the first time I have not included verm in my sub. When I started, coir\verm seemed to be the standard so I just continued with what I knew. I just didn't have any verm this time so figured I would finally join the rest in omitting it. This is admittedly not as flashy or detailed as my usual updates. That is hopefully another thing I will do better on in the coming progress updates. ________________________________ ________________________________________________________________________________________________________________________
A bit out of order and context as I don't remember which pic are from which plates but....... YAY MYCROSCOPY!!!!
    
Edited by GypsyBastard (02/25/21 05:45 PM)
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Leon Forest 
Registered: 02/11/13
Posts: 574
Last seen: 3 months, 18 days
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: GypsyBastard]
#27103189 - 12/22/20 09:58 PM (3 years, 3 months ago) |
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Indulgence
Starting from swabs
Species: P. cubensis
Variant: Pearly gates
12/31/20
Plates poured, swab ready. Used the other swab to start a few plates last week so I really hope to get a good plate from this one or I'm out.
01/01/21
Box is ready
Plates are swabbed.
01/02/21
I've decided to add 2 more into the mix. I will also be doing Tat and p. allenii
1/22/21
Update, these are all the plates I got to germinate from pearly gates. Nothing from tat or allenii yet. Very old spores on those so they may not germinate.
Edited by Leon Forest (01/21/21 10:23 PM)
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GypsyBastard
Misanthrope
Registered: 11/30/20
Posts: 846
Loc: The Mighty Boosh
Last seen: 3 years, 1 month
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Leon Forest]
#27103200 - 12/22/20 10:02 PM (3 years, 3 months ago) |
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Fuck yeah, Welcome aboard!!
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TigerStrike
carpophore locator
Registered: 12/22/20
Posts: 315
Last seen: 1 year, 11 months
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: GypsyBastard]
#27103205 - 12/22/20 10:06 PM (3 years, 3 months ago) |
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I reserve my spot. for jan 1st. Prepares slurry LC to speed up germ muwhahahahhahhahahha
OH shit start from spores. Damn 5th place here I come!
All species and varieties welcome!
I dont have any spores as i am in ID and dont know how to find and its the wrong time of year and gainesville FL is prob too cold too far so I will be growing whatever randon mushroom i find jan 1st and will print and grow it! Now i need SAB, agar, PC and thats it!
I
Edited by TigerStrike (12/22/20 10:10 PM)
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GypsyBastard
Misanthrope
Registered: 11/30/20
Posts: 846
Loc: The Mighty Boosh
Last seen: 3 years, 1 month
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: TigerStrike]
#27103212 - 12/22/20 10:12 PM (3 years, 3 months ago) |
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Yep. Any variant of any species. Could be cubes, could be pans......
Can be from a print or a MSS or a swab and can use as many different variants/species as you feel like keeping up with.
Only 2 restrictions:
Must start with spores on agar
and
Can't start before 00:00.01 on 01\01\2021
Starting late is totally fine as this experience is meant to be about learning together and don't want people to miss out on that because they weren't ready in time. 
Just to clarify, this is not my thing so I'm just following the rule laid out by those before me. Admins and TC can make any judgment calls that need to be made if it gets to that point.
There may be some contests/prizes offered up as we go but I don't any specifics as of yet.
Edited by GypsyBastard (12/22/20 10:22 PM)
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TigerStrike
carpophore locator
Registered: 12/22/20
Posts: 315
Last seen: 1 year, 11 months
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: GypsyBastard]
#27103216 - 12/22/20 10:15 PM (3 years, 3 months ago) |
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Wait it doesn't say anything about active species which I probably wont be able to find.
I am planning on grabbing the first lactarius indigo or bolette or something similar.
May I please still enter with a random non active mushroom if I can't find an active one?
Edited by TigerStrike (12/22/20 10:17 PM)
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GypsyBastard
Misanthrope
Registered: 11/30/20
Posts: 846
Loc: The Mighty Boosh
Last seen: 3 years, 1 month
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: TigerStrike]
#27103219 - 12/22/20 10:21 PM (3 years, 3 months ago) |
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Quote:
TigerStrike said:
May I please still enter with a random non active mushroom if I can't find an active one?
Absolutely!
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 bigfootscreepyuncl
Stranger
Registered: 11/15/20
Posts: 3,733
Loc: Gamehenge
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: TigerStrike] 1
#27103232 - 12/22/20 10:28 PM (3 years, 3 months ago) |
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 BIGFOOTS 2021 GROW-ALONGSpecies: P. cubensis
Varieties: Golden Teacher, Albino Penis Envy, Penis Envy12/29/20 Poured my plates today. 400ml undiluted grain-soak water with 8g agar-agar (telephone brand). I need to start using a towel or something under my SAB floor because it dips in the middle, causing VERY uneven pours resulting in not many plates to be poured. 400ml got me 15 plates. SAB arm-holes are loosely covered with paper towels. I know it's considered un-necessary but the way my 'lab' is set up air from my central heating gets blasted straight into it from across the room. Poured at 118-120F and stacked poured plates 15 high in an attempt to beat condensation at its' own game. Pictures to come after inoculation.   1/1/21 Got my plates inoculated today. 5 each of GT, PE and APE. I think my sterile procedure was pretty good but I'm hungover and shaky as fuck so time will tell! These are my clearest plates yet with very little condensation. Baby steps! 1/7/21 We have germination! After nearly a week of checking on my plates several times a day, posting on this thread and undoubtedly pissing off some of the people following along with my complaining I finally noticed some baby mycelium on one of my APE plates! Out of my GT, PE and APE I wasn't expecting to get anything out of this one in particular.   Sorry for the clarity of the pictures. I poured some crystal clear plates, got impatient so I moved my stack closer to my heat vent and that condensed them like a bastard...live and learn. The closeup was attempted to be taken through my jewelers loop. Cheers, mates! 1/15/21 Today was an interesting day. While my BRF pucks were in the PC I decided to give my plates one last look before tossing them after sitting blank for 14 days. The one APE plate I had that germinated has sprouted a tiny spec of myc in another spot and one of my PE plates finally germinated!! I need my jewelers loop to see it, but it's there! Still waiting on GT, but I think these BRF cakes will do the trick. Anyway, I inoculated the BRF cakes (3 GT, 3 PE and 1 APE), took 8 T-1 transfers off my APE plate and did a couple transfers for a non LAGM project. Busy day and it felt good! Shout out to d3monic for his agar TEK...absolute fucking GLASS!   1/26/21 After wasting a sleeve or two of plates that wouldn't germinate I said fuck it and made a batch of BRF pucks. This is LAGM and desperate times call for desperate measures! My GT and PE finally got off their lazy asses and germinated. I took transfers from the pucks to agar on 1/22/21 and just made the T-2 tonight. These are the T-1s from the pucks: GT  PE  PE  I was awfully tempted to send this one to LC but decided I'd better at least make another transfer. My hands got shaky as fuck when taking these transfers so hopefully I didn't jack this one up. 1/28/21 My APE is crawling along, to be expected. APE T-1   APE T-2  PE T-2  Ultra clear plates for this batch! I think these will go to grain/LC
Edited by bigfootscreepyuncl (01/28/21 01:08 PM)
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TigerStrike
carpophore locator
Registered: 12/22/20
Posts: 315
Last seen: 1 year, 11 months
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: GypsyBastard]
#27103237 - 12/22/20 10:31 PM (3 years, 3 months ago) |
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BAfucakingam I'M GONNA BE THE BEST fucking pokemon Trai. I mean Mycologist in the entire worlld!  It will be my first wild species print to agar. Honestly it will be my first time agar anyhing but I am a fucking GURU on RR's vids. I watched all of them like 4 times.
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GypsyBastard
Misanthrope
Registered: 11/30/20
Posts: 846
Loc: The Mighty Boosh
Last seen: 3 years, 1 month
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: TigerStrike]
#27103251 - 12/22/20 10:42 PM (3 years, 3 months ago) |
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You're in!!
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alaskappalachian
Entitiologist
Registered: 10/22/19
Posts: 1,758
Loc: The 49th Dimension
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: GypsyBastard]
#27103291 - 12/22/20 11:09 PM (3 years, 3 months ago) |
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Alaska's LAGM2021 Big Golden Grow Log  We'll be growing:~Psilocybe cubensis: Golden Halo ~~Psilocybe caerulescens: GA/wild~
~Bonus Exotic: Psilocybe semilanceata: EU/wild~
~Bonus Cube: AA+~
 
(^^source material^^)
Golden Halo
31Dec20 16:30: our time and I'm running like 15 hodgepodge prepours in the lil 16qt on the woodstove. I just ran and used 50 over the last two days so just a few to get by for now...
For some time now my usual agar recipe has been:
-10g agar
-8g brf (home-ground, and poorly so might I add...)
-500ml H2O
I add my brf to the water and let it sit a while, occasionally agitating. I don't filter or decant or anything. Not here to make beauty plates for the Gram. Agar added cold after the brf soaks a little while, then brought to boil and poured. Dishes allowed to cool sans lids, assembled (almost all my prepours now are jars with unmodified lids), and PCd for 30min @15psi (or steamed an hour, depending on what's going on)...
3Jan21 00:30:
FINALLY streaked all my plates tonight:
-3x semilanceata
-2xGHalo
-2×caerulescens
-2×AA+
7Jan21: Still waiting on germ for all var/sp. Went from -20f out/40f in at night to 40f out/75f in at night now so hopefully that spells good things. Usually G Halo is fast AF to germ. Actually, caerulescens had been for me in the past too. 
8Jan21
Golden Halo and AA+ showing germination.
9Jan21
Each GH and AA+ got 2x xfers (4x total of each).
10Jan21
Semilanceata and caerulescens both showing clear germination. Making more prepours today. 
22Jan21
Ok... so last week I made some agar and somehow the fuck or another fucked up my nutes/math. The resultant plates showed clear, derpy growth that I had to wait until it grew out until I could proceed with photos. My semilanceata and caerulescens are just now growing back out, as is the AA+ (enough to show photos of). The GH was its usual bonkers resilient to my whoopsie, and started to throw its trademark rhizo growth after some time.
Took 2 xfers from this plate and may go to grain with a couple wedges for fun. The blips you see in the center are bits of poorly ground brown rice, for the record.
31Jan21
Long time no update, but I am almost ready to take the semilanceata to grain. Hard to see with prepours but you get the idea. We're looking good. Temps inside are... really low lately. so progress is painfully slow. Might buy some RGS for this project but the car's head gasket is toast. Have to make it up to H Depot and check er out or might just have to run barley. Hmmmm... 
10Feb21
1st caerulescens plate that looked decent enough to drop so dropped it as well as the best semilanceata plate to 4 qts (2 each)of Barley. Prep was overnight soak, followed by 50min simmer/boil.
12Feb21
Mixing up sub for bottle grows (semilanceata). Not sure the exact ratios but in order of amount: manure/straw/coir/wbs/bran. Mixed last night, PCing today. Dropped caerulescens and semi cultures to jars of barley (2x qts for both) night before last (10Feb21).
15Feb21
Inoculated 8x pts of semilanceata substrate with ~4cc of liquid inoculant (made from tossing a puck in a jar of sterilized water and razor blade and agitating). Mix was well-hydrated and yet shook well/freely. One jar at least will contaminate I'm sure given that while I was wiping down my lid,I accidentally jerked and plunged my finger and iso rag inside and touched the substrate... 
18Feb21
 
Semilanceata and caerulescens jumping off onto barley.
20Feb21
Didn't have propane cash for stove but I was itching to cult, so I made 14 WBR 1/2 pints. WBR Prep: water brought to ~200f and rice stirred in. Brought to boil and kept at low boil for maybe 8 -10 min. I always follow the initial draining with a boiling water rinse. You want it to swell but retain it's shape (not "bloom").
I made a LI with the best looking T3 AA+ plate and inoculated all with ~3cc.
1Mar21
 
Photos taken maybe 2-3 days ago(?). Example of visible germination now evident on all semi bottle grows and grain jars. Cold snap (pretty darn chilly indoors, even for Alaska) really slowed everything down so I was happy to see this. Will monitor and report. 
3Mar21
Finally got more Halo cultures going and first quart taking off nicely. I'll expand to 4-5 more for a 64qt likely. 3 more plates will be ready for grain by Fridayish.
11Mar21
Shook first qt of Halo today, and made a LI out of this plate to inoculate 5x bottle grows (4xqt and 1xpt):
If they don't contaminate I'll be the luckiest guy in the world. Wife wanted the DSLR I was using in the SAB and got me irritated and I had an... angry air box session... during the LI making. Never know. Mix for bottles was something like 50% manure, 10% coir, 30% WBR, 10% wheat bran.
Semi bottle grows are coming along finally after cold-weather lag.
8 of my (AA+) WBR half pints are colonized so going to a 16qt tonight likely. 6 more @90% or so. Might do a novelty.
13Mar21
Made 3shoeboxes eventually from 13 cakes (4/4/5).Not enough room in my space for larger tubs or expansion. Getting submarine level cramped in here...  Remaining two half pints will be used for a novelty. Halo qt and semi qts are chugging along.
Edited by alaskappalachian (03/14/21 04:47 PM)
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mushhead
Potato Devourer
Registered: 08/22/14
Posts: 2,224
Loc: Dimension H-231
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: alaskappalachian] 1
#27103343 - 12/23/20 12:24 AM (3 years, 3 months ago) |
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OH hell YES !!!!!!!!!
P. Cubensis Burma strain
&
Pan Cinctulus
(Since Cints grow wild here I'll be doing both an indoor and an outdoor bed of them)
Spores go to agar midnight of the new year m8's
EDIT:
Shout out to alaskappalachian for the Pan Cincts!!
EDIT 01/08/21:
Getting stacked for LAGM21
EDIT 01/12/2021
This Burma cubensis culture was start 01/01/2021 and got one transfer onto the plate bellow.
Germ plate then straight from t1 to grain.
 UPDATE 01/15/21  Agar has slowed down after being dropped to grain.
 Update 1/18/21 Looks like the myc has finally taken ahold of the grain. Woot woot chicken foot.
 It's coming along well.
I forgot to shake my jar about five days ago, but took pics before I did.
UPDATE TIME!! 01/21/2021
 
This is usually when I shake my jars, but I do like to do it a bit sooner.
UPDATE 01/25/2021
We're starting to see cohesion, the myc really love this winter wheat.
UPDATE 01/26/2021
Woot WOOT chicken FOOT This myc is moving so fast. Thank you Burma.
I love Burma Cubensis.
 
It's almost time for G2G 01/28/2021
EDIT: I forgot to post yesterdays pic
this is from 01/27/2021.
Also sorry about the Pan. Cinctulus, I haven't even spread spore to agar with those yet.
I will soon do not worry!! It is coming time when I must plan my outdoor bed of them.
First jars of Burma variant cubensis onto grain.
Next up g2g.
--------------------
Meditation Principles
Silence: Giving you room to listen / Stillness: Giving you room to feel / Spaciousness: Just giving you room
IRC: ##mycology on Libera.chat come hang!
Edited by mushhead (01/28/21 02:52 PM)
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alaskappalachian
Entitiologist
Registered: 10/22/19
Posts: 1,758
Loc: The 49th Dimension
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: mushhead] 1
#27103345 - 12/23/20 12:33 AM (3 years, 3 months ago) |
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--------------------
THE 49TH MYCOJOURNAL: EXOTICS, AURORAS, & ENTITIES
ISO:Gym punctifolius spores for trade 
Edited by alaskappalachian (12/26/20 04:13 PM)
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 Haywire
Wetspot Wizard
Registered: 12/29/13
Posts: 1,619
Last seen: 9 minutes, 5 seconds
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: alaskappalachian]
#27103370 - 12/23/20 01:01 AM (3 years, 3 months ago) |
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Z
made a couple transfers from germination plate and then inoculated a grass seed substrate.
grass seed prep: put dry seed in container with a pinch of gypsum, add water, sterilize for 60 minutes.
I waited until I got in vitro pins/fruiting bodies and cloned those.
   
My pin clones made pins so I cloned those:
 
Next step: grain spawn!
Edited by Haywire (05/11/21 07:02 AM)
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MH5109
Stranger
Registered: 09/27/17
Posts: 1,385
Loc: America 
Last seen: 3 years, 2 months
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Haywire]
#27103431 - 12/23/20 02:39 AM (3 years, 3 months ago) |
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AA mss, I don't have AA in the rotation and I miss it. Looking forward to getting my shit together in life and coming back to cult.
1/2: AA Spores to agar
1/9
Updating with what was a terribly bacterial syringe, but it looks like the myc is outgrowing it but only time will tell. Im going to biopsy a few plates tomorrow and see if I can get the myc away.
 
1/28
Got into two transfers since last update. This plate looks like I'm getting away from the original contamination. I plan on taking a couple more transfers and going to grain.
 
--------------------
Edited by MH5109 (01/28/21 12:35 AM)
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GrinchGrower
N00B
Registered: 10/02/20
Posts: 682
Last seen: 2 years, 11 months
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Haywire] 1
#27103437 - 12/23/20 02:47 AM (3 years, 3 months ago) |
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GG LAGM 2021
Species: P. cubensis
Variant: Escondido- 12/24/20: Printing
Escondido cap printed and stored for LAGM 2021
____________- 01/01/21: Inoculation
Thirty-eight LME plates poured for the next few weeks
(20g agar/16g LME/1000ml deionized H2O)
  
Scraped spores with sterile scalpel to five agar plates
(~3 hours after pouring in SAB)
____________- 01/05/21: Germination
    
Germination taking place on five plates
____________- 01/08/21: T-1
Took transfers from five germ plates
____________- 01/13/21: T-1 Update
    
Growth looks okay so far
____________- 01/18/21: T-2
 
Took two transfers from five T-1 plates
Cracked bottom of one parafilming 
____________- 01/26/21: T-3
Took five transfers from select plates
____________- 02/04/21: T-3 Update
 
Growth starting to look better
____________- 02/13/21: T-4
  
Took emergency transfers from moldy T-3 plate 02/09/21
 
Split promising T-3 plate in half
Inoculated two jars of rye
 
Took some final transfers from two T-3 plates
____________- 03/03/21: A2G
    
Sent five plates to quarts of rye
____________- 03/12/21: Spawn / Shake
One jar inoculated on 02/12/21 ready to spawn
Made a shoebox today, will update every 10 days or so
Other jars that were inoculated 03/03/21 got shaken too
Forgot to take pictures
____________- 03/25/21: Pinning
Shoebox spawned 03/12/21 started pinning 
Final batch of jars will be ready to spawn soon
____________
Happy New Year, Shroomerites!
Edited by GrinchGrower (03/25/21 06:30 PM)
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tiptrippy
The Mechanic
Registered: 09/09/20
Posts: 1,131
Loc: United States
Last seen: 1 year, 8 months
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: GrinchGrower] 1
#27103450 - 12/23/20 03:04 AM (3 years, 3 months ago) |
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Edited by tiptrippy (01/29/21 01:00 AM)
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BrownBear
Warrior-Traveler
Registered: 06/05/20
Posts: 1,539
Last seen: 10 months, 22 days
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: tiptrippy] 1
#27103469 - 12/23/20 03:26 AM (3 years, 3 months ago) |
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This should be fun.
RustyWhyte 8 plates swabbed from a print.
1/1/21
Edited by BrownBear (01/01/21 08:50 AM)
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The Dalcassian
Dirty Druggie
Registered: 01/04/17
Posts: 952
Loc: Emerald lsles
Last seen: 11 months, 20 days
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: tiptrippy]
#27103474 - 12/23/20 03:30 AM (3 years, 3 months ago) |
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Ya, go on so.
  Not So TAT Pan Cyan Wild CoastSpecies: Cubensis
Variety: Not So TAT (NST)
Method: Swab to agar
Inoculation: 01/01/21
Species: Panaeolus Cyanescens
Variety: Wild coast
Method: Scalpel to agar
Inoculation: 01/01/2101/01/21 @ 00.35am- spores to germ plates, 10g lme, 8g agar and 500ml water 01/03/21Not So TAT - germination has happened! Very faint hyphae visible on one plate. 01/04/21Pan Cyan Wild coast - Germination on one plate 01/09/21Not So TAT - T1 Xfer - took 6 xfers to 2 plates 01/09/21Pan Cyan Wild coast - T1 Xfer - took 5 xfers to 2 plates. If I get good growth I could potentially go to grains next week!!! 01/14/21Not So TAT - Took 2 xfers to their own plates. If the growth is clean and I can avoid another satellite, (little bastard)  I'll nocc up a jar next.  01/14/21Pan Cyan Wild coast - Here's a pic of one of my T1 plates. The better of two.  Also chucked a couple of wedges from the germ plate into grain jar. Yolo 01/16/21Not So TAT - Took a T1 colony to grain out of boredom but it's looks good and clean so why not. It the colony at 12'  01/16/21Pan Cyan Wild coast - More experienced cultists than I have questioned the growth pattern of my myc. So I've nocc'd up two fresh plates to see if I can increase my options 02/07/21Not So TAT - Well that T1 i chucked to grains did really well, so I've expanded it to 6 quarts today. Wont be long until spawning. Also have a really nice T3 but ill refrigerate that for now 02/07/21Pan Cyan Wild coast - well I've got Alabama and Wild Coast on agar and looking good. I think the Alabama will be ready first though. 02/19/21Not So TAT - Well I've got 5 quarts almost ready for spawning. Probably the next day or two I would think  02/19/21Pan Cyan Wild coast - I dunno whats happening here, I've got stalled Alabamas and freshly nocc'd WC jars atm. All is well on agar then they just crawl and go to sleep on grain   p
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