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BrownBear
Warrior-Traveler
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: tgriff02]
#27224174 - 02/24/21 02:45 PM (3 years, 1 month ago) |
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Everytime I have used a spore syringe on an agar dish, it always ends up looking like a mess. I am pretty good at dripping one drop at a time also. Do you have any pictures to share of your cobweb contaminated plates?
I have wg, ess and apeu swabs. If you are interested in any of those just me a privnote of your addy.
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Gastronomicus
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: tgriff02]
#27224175 - 02/24/21 02:45 PM (3 years, 1 month ago) |
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Sounds like it. How much are you using per plate? Ideally with a syringe you want to put down a single drop and then swipe it around in a Z pattern. If all of your PF Classics contamed then yeah sounds like you got a bad syringe. You're inoculating in a Still Air Box or with a flow hood right?
-------------------- Make my Funk the P Funk, I wants to get Funked up LAGM2024
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A.k.a
Stranger
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: tgriff02]
#27224177 - 02/24/21 02:46 PM (3 years, 1 month ago) |
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Yep. Shitty syringe.
Better plates than a bunch of cakes though. If you have agar now try to get your hands on a print.
-------------------- LAGM2020
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tgriff02
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: A.k.a]
#27224210 - 02/24/21 03:03 PM (3 years, 1 month ago) |
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Unfortunately I do not have any pics and I chunk anything the moment I see green or black. I live in an older house (original construction actually predated the official founding of the county I live in) so I'm really, REALLY panicky about keeping the shroom stuff clean.
I expect a spore syringe to spawn with contaminates, that's one of the main points of agar, as I understand it, because it has the benefit of being able to transfer away from agar. The discouragement is coming from the fact that so far I have spawned NOTHING except mold with exception of that one B+ plate that is currently on T2 working on isolation. But yeah. I'm currently working with B+, GT, PE, and CRS (just because it's kinda fun).
When I do a plate, I put a single drop on the center of the plate and use a flame sterilized loop to streak in a Z pattern, rotate the plate 90degrees, then streak it again.
And yes, I do it inside a SAB. Current procedure is to close of the shroom room, spray a fuck-ton of lysol/spray disinfectant, let that settle while I get a shower and change clothes. come back and set up the SAB and spray a couple shots of soapwater, then wait about 5 minutes while I wash my hands and forearms again, put on latex gloves and then wipe down my forearms and hands/gloves with 70% iso. Then I flame sterilize all my tools before placing them in the SAB. So far I have not yet had a test plate contaminate, so I'm feeling fairly comfortable with my sterile procedure.
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tgriff02
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: A.k.a]
#27224216 - 02/24/21 03:06 PM (3 years, 1 month ago) |
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Quote:
A.k.a said: Yep. Shitty syringe.
Better plates than a bunch of cakes though. If you have agar now try to get your hands on a print.
I do have some B+ and GT spore prints from last fall, I may try that. Never managed to get any print from PE or my CRS last fall before I had to put cultivating on hold. Should have quoted this in the other post. Atm I am broke and have nothing to offer in trade; so I feel bad about asking for prints of either of them just yet.
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Gastronomicus
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: tgriff02]
#27224218 - 02/24/21 03:08 PM (3 years, 1 month ago) |
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Day 13, shook 2 of the millet jars. The 3rd is either super slow or the agar is mostly in the middle and there's a lot of colonization I can't see. Gonna give it a few more days before I shake again. Gotta pour more plates but I'm working so much these days. Maybe I'll try to whip some up while I watch hockey tonight
-------------------- Make my Funk the P Funk, I wants to get Funked up LAGM2024
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A.k.a
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Gastronomicus]
#27224223 - 02/24/21 03:10 PM (3 years, 1 month ago) |
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Don’t worry so much about a dirty house. I did the same thing at first but it doesn’t make much difference if you’re using the SAB right.
-------------------- LAGM2020
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roarkell
The Roar Master!
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: tgriff02]
#27224319 - 02/24/21 04:08 PM (3 years, 1 month ago) |
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Quote:
tgriff02 said:
Quote:
A.k.a said: Yep. Shitty syringe.
Better plates than a bunch of cakes though. If you have agar now try to get your hands on a print.
I do have some B+ and GT spore prints from last fall, I may try that. Never managed to get any print from PE or my CRS last fall before I had to put cultivating on hold. Should have quoted this in the other post. Atm I am broke and have nothing to offer in trade; so I feel bad about asking for prints of either of them just yet.
Take a look at my trade list in my sig... ill hook ya up with anything on there...
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Ambrose
Registered: 01/16/20
Posts: 541
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: roarkell]
#27224569 - 02/24/21 06:17 PM (3 years, 1 month ago) |
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bruh. Second tray of chicon coming in weird af. idk if the difference can be attributed to MS or conditions or wtf...
Bottom line is, mofos do not look healthy. Just dat noob life I guess.
I increased the light and FAE to see if that will get the fuckers to stop growing wacked out:
At least they fruited I suppose
Edited by Ambrose (02/25/21 08:50 AM)
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A.k.a
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Ambrose]
#27224581 - 02/24/21 06:22 PM (3 years, 1 month ago) |
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I think genetics are huge for stone producers. I’ve had a lot that look similar to your current tray, that first one is just 10/10 crazy uniform with great caps. Still working on one of those myself
-------------------- LAGM2020
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Ballzagna
Carb Daddy
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: A.k.a]
#27225023 - 02/24/21 11:07 PM (3 years, 1 month ago) |
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Yeti pins from the one germ plate
Yesterday morning and tonight
-------------------- No one likes a naysayer It's probably fine. Even if it's not fine, it'll eventually be fine. So it's fine. PE Adventure Photosynthesis: A Morning Ritual Ballzagna's Tek Compendium and Notes for Noobs
Edited by Ballzagna (02/24/21 11:22 PM)
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GypsyBastard
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Ballzagna]
#27226314 - 02/25/21 05:42 PM (3 years, 1 month ago) |
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02\25\2021
Forgot to get pics before shaking. Will try to be better about getting pics are the grow progresses.
Started today: 5 shoeboxes (AA+ & PFC) 1 mini(PFC)
Started previously: 1 shoebox (AA+)
Not sure where I went wrong.... I know I started more than 2 variants but somehow along the way I seem to have forgotten about some and am now only working with AA+ and PF Classic
There is still a RustyWhyte germ plate to working and some z plates I can transfer from. Not sure what the hell went wrong with my GT. It was my go-to for years but having just terrible growth and contam issues with my print this time around. May just dig out a fresh print and try again. They hold a nostalgic place in my heart and don't want to lose that line, but LAGM has highlighted some limitations I have with time and work space so I may wait a bit to restart those. Just the 6 shoeboxes, 1 mini, and a handful of cakes is proving to be more of a hassle than I would like currently and would rather grow a few tubs well, rather than a bunch poorly.
Each shoeboxes have roughly 2qt coir and varying amounts of spawn. Some are 1:4, some 1:2. Mini holds about 3qt coir (one of the first I ever made and holes are a bit lower than they should be so it's basically just a tall shoebox) and has a spawn ratio of around 2:3. The one shoebox started previously is about a week along (will check dates later) and is looking pretty close to pinning. My confidence in the cleanliness of the spawn is not as high as I would like. It was the 1 jar of three that didn't not show obvious signs of contams. However, all three jars were noc'd with the same plate. I gave it an extra shake and plenty of time for them to show up and did not see any deal breakers but still know that it is less than ideal.
This is actually the first time I have not included verm in my sub. When I started, coir\verm seemed to be the standard so I just continued with what I knew. I just didn't have any verm this time so figured I would finally join the rest in omitting it.
This is admittedly not as flashy or detailed as my usual updates. That is hopefully another thing I will do better on in the coming progress updates.
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mushboy
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Loc: where?
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: GypsyBastard]
#27226478 - 02/25/21 07:22 PM (3 years, 1 month ago) |
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Inoculated 2 grain masters with two different pe ms cultures
I also made some li but I forgot that I need either softer agar or a sterile blender attachment. Hopefully I can do that without contaminating everything
Pans are finally chugging along I'll post pics and make a real post soon. The lazy is strong in my family.
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capntrips7
Moldsmith
Registered: 10/21/20
Posts: 91
Loc: Urithiru
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: capntrips7]
#27226515 - 02/25/21 07:44 PM (3 years, 1 month ago) |
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Finally caught up on my log:
Quote:
capntrips7 said:
Day 32, 2021-02-03
Made AA+ spore syringe.
Inoculated plate 31.
Made transfers to plates 32-36.
Inoculated jars 10-15.
Cased leftover grain from jar 5.
Boiled water, poured over coir, mixed after 1 hour.
Prepped tub 1 with 396g spawn and 989g substrate, cased with 155g coir.
Day 52, 2021-02-23
Inoculated plates 37-38.
Made transfers to plates 39-42.
Day 53, 2021-02-24
Inoculated plates 43-44.
Day 54, 2021-02-25
Inoculated plates 45-46.
Boiled 813ml water, poured over 163g coir, and mixed immediately, reserving 60g.
Prepped tub 2 with 486g spawn and 829g substrate, cased with 60g coir.
Here are some progress pics of the shoebox I spawned on 2021-02-03 (days 8, 11, 20, and 22 after spawn):
I've been fighting cobweb with H2O2. We'll see how it goes. I'll try to post updates.
Also, over the last few days, I have taken some T2 transfers of GT, APE, and RW and inoculated agar from spores of some new varieties: AA+ (again), Avery's Albino, Plantasia Mystery, Star Gazers, and Huautla/Oaxaca.
-------------------- LAGM2021
Edited by capntrips7 (02/25/21 08:06 PM)
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Gastronomicus
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: capntrips7]
#27226638 - 02/25/21 09:15 PM (3 years, 1 month ago) |
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Poured new plates but got distracted half way through by work. Hoping I didn't contam them going in and out of the SAB
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BrownBear
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Gastronomicus]
#27226646 - 02/25/21 09:22 PM (3 years, 1 month ago) |
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You should be alright as long as you had all of your plates wrapped up in between SAB sessions.
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coversall
إِنْ شَاءَ ٱللَهُ
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Posts: 2,749
Loc: संसार
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: BrownBear]
#27226951 - 02/26/21 03:53 AM (3 years, 1 month ago) |
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~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
26.02.21
Got my arse into gear and cased the 5 pan cyan BVI pan-cakes. Popped in the FC and fired it up. Fingers crossed.
I'm tempted to streak some more BVI as this whole grow has left me with the feeling the I selected a shitty culture. Looking back at myc pics from my pan cyan texas grow has really drilled this home. The way covid restrictions are panning (ahahaha) out means I think I've got enough free time to start up again. If I'm a bit more on top of things I reckon I'll squeeze it in. If I do I'll pour and streak on monday.
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Edited by coversall (02/26/21 04:13 AM)
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roarkell
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: coversall]
#27226959 - 02/26/21 04:00 AM (3 years, 1 month ago) |
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Man... ive really been slacking over here... im still in this, I think most of my entries are either on grain or in shoeboxes already... I honestly don't even remember what I started out with... hoping I can take some pics and do one big update this weekend sometime...
Keep at it boys and girls!!
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ReverendMyc
Registered: 03/29/19
Posts: 1,750
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: roarkell]
#27227453 - 02/26/21 12:05 PM (3 years, 1 month ago) |
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Taking off for the weekend so dropping a quick update.
MuthaFudpucker Grows some stuff (update) 2021-02-26 2 of 6 PE inoculated on the 22nd 2 of 10 Tamps inoculated on the 22nd Will probably shake both on Monday. OP updated.
-------------------- LAGM 2.024Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any more"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023 Don't Panic
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Tankie_J
I like having fun
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Posts: 537
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: ReverendMyc]
#27227546 - 02/26/21 12:50 PM (3 years, 1 month ago) |
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Have a fun weekend! Where did you find those lids? I’ve had a hell of a time sourcing them... seller on Amazon wants $30 for 16 of the ball lids, does that sound right?
Edit: This question goes out to anyone with info on this.
Edited by Tankie_J (02/26/21 12:51 PM)
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