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GypsyBastard
Misanthrope


Registered: 11/30/20
Posts: 846
Loc: The Mighty Boosh
Last seen: 2 years, 10 months
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: falconman]
#27176296 - 01/29/21 01:56 PM (2 years, 11 months ago) |
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Quote:
falconman said: Is it too late to join in? I have some variety I started a few weeks ago and wanna document with the bois!
Yeah man I'll get you added in the OP later on.
Doctor Mario, just double checked and my scope was less than I thought. It's a Telmu (which seems to be the cheap AmScope and was only like $75. I feel like for what I'm doing with it I really can't complain too much. I'm pretty happy with it.
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ReverendMyc

Registered: 03/29/19
Posts: 1,496
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: LuxFlux]
#27176297 - 01/29/21 01:56 PM (2 years, 11 months ago) |
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-------------------- LAGM 2.024Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any moreHow to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice. Don't Panic   
Edited by ReverendMyc (01/29/21 01:59 PM)
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Ambrose

Registered: 01/16/20
Posts: 518
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: ReverendMyc]
#27176365 - 01/29/21 02:36 PM (2 years, 11 months ago) |
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Quote:
MuthaFudpucker said: MuthaFudpucker Grows some stuff (hopefully)
2021-01-29
Tamps: the three that are technically T2s but they completely stalled on some bad agar. Seems to have recovered on the new plates.
  
and a side angle of 206 trying to get the shape. The Tamp transfers have all been little puff balls at first, then settle down on the agar and spread out. I think that AKA mentioned his Tamps doing the same thing in another thread. The picture doesn't quite capture it.

These are Tamps T1s because I went back to the germination plates because I didn't trust the others to recover.
  
Looking good! Look like they'll be ready for grain soon! They tend to be puffballs then flatten for me as well. What was the agar recipe that stalled them?
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ReverendMyc

Registered: 03/29/19
Posts: 1,496
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Ambrose]
#27176431 - 01/29/21 03:15 PM (2 years, 11 months ago) |
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@Ambrose I thought was the same as normal but with some of the same charcoal that D3 uses instead of the food coloring I usually use. I must have done something wrong though. Nothing that I put on it did well at all.
So as you can see, I over compensated and didn't use any color at all this time. I don't think the charcoal was the issue. I am sure I messed something else up.
-------------------- LAGM 2.024Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any moreHow to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice. Don't Panic   
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falconman
::absorbing info::


Registered: 07/22/20
Posts: 129
Last seen: 2 years, 2 months
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: GypsyBastard]
#27176719 - 01/29/21 05:34 PM (2 years, 11 months ago) |
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Quote:
GypsyBastard said:
Quote:
falconman said: Is it too late to join in? I have some variety I started a few weeks ago and wanna document with the bois!
Yeah man I'll get you added in the OP later on.
put me up for B+ and Mazatapec! I can't remember the exact date of t0 but it was sometime between 1/3 and 1/10
-------------------- If any of my posted info is incorrect or questionable,please DO correct me. I strive to always learn new or better ways and information Grower off all things nature! things currently growing I am proud of: 50+ trichocereus named cultivars B. Caapi that has died and came back twice 30+ varieties of herbs, berries, beans, and vegetables psilocybe cubensis
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Ambrose

Registered: 01/16/20
Posts: 518
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: falconman]
#27177183 - 01/29/21 11:17 PM (2 years, 11 months ago) |
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Chicon looks to be just about fully colonized at day 13:
 Hope to spawn it this weekend to straight coir 1:1
 First time using WBS, really liking it over oats so far. Cant seem to get oat prep right
Edited by Ambrose (01/29/21 11:20 PM)
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Doctor Mario
🍄🍄🍄🍄🍄



Registered: 08/07/20
Posts: 1,894
Loc: 🇺🇸
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Ambrose]
#27177225 - 01/30/21 12:00 AM (2 years, 11 months ago) |
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Quote:
A.k.a said: There’s at least one vendor who makes clean syringes. Anywhere with PE syringes/syringes with dense spore balls will prob be ok because they’re made with a machine that separates bacteria and mold spores from the cubes.
I did syringe to grain 20 times when I started and never had a problem. I was expecting to lose most of them so I did end up with way more mushrooms than planned 
I even made clean lc from them.
This isn’t to say it’s the best way to grow, agar is definitely necessary for anything more than a minor hobby, but spore to grain has a very high success rate if you get the right stuff.
What kind of machine is it? Thats pretty bad ass and I wanna know more. Are they just centrifuging it or something?
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 3 hours, 51 minutes
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Doctor Mario]
#27177251 - 01/30/21 12:39 AM (2 years, 11 months ago) |
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Yeah centrifuge that’s what I read.
I assume there’s a series of gradually smaller filters everything is forced through so there’ll be like a chamber of just cube spores.
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LAGM2020     
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PitcherCrab
Crescent Fresh



Registered: 12/02/06
Posts: 2,446
Loc: The bottom of the sea.
Last seen: 1 year, 1 month
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: A.k.a] 2
#27177259 - 01/30/21 12:45 AM (2 years, 11 months ago) |
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1/29/2021
I finally have some really good looking mycelium to work with for both the TOC and TWC. I'll be transferring one more time then it's to grain!
Here are the plates I'm liking:
-------------------- PC's LAGM 2021 (TOC & TWC)P. natalensis Growlog 2021Pans for PC Fall 2021 Growlog “I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear
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Narrator
Strangest in the night



Registered: 11/30/20
Posts: 187
Last seen: 1 year, 5 months
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: A.k.a]
#27177342 - 01/30/21 03:00 AM (2 years, 11 months ago) |
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1.29 APE T2s, hoping to get ahead of the badies.
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jay.ach
Traveler



Registered: 05/25/19
Posts: 531
Loc: USDA Zone 8
Last seen: 1 hour, 50 minutes
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Narrator]
#27177487 - 01/30/21 07:05 AM (2 years, 11 months ago) |
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Wow! Everybody is killing it! And @LuxFlux - one thing this journey will teach you is patience. And it's great you are posting and asking questions. Keep working it! Here's my update:
1/30/2021 Week 04 Check in... T-1s on WBS after 7 days

The wild bird seed still looks wet but the Myc likes it. Good growth. Once the Myc in the jar on the left reaches the top of the seed, I'll shake the jars.
I am also interested to see what happens with the myc hits that sunflower seed on the left. If it colonizes okay, I'm going to try a couple of jars using all black sunflower seeds.
My plates from my Tribe mystery bill print are showing some growth but I can't get a good picture yet.
And my B+ T2 backup plates are not looking symmetrical. I'll mix up more Agar tonight and transfer to T3s. These will only be used if my WBS doesn't make to spawning
See you next week!
-------------------- “One's destination is never a place, but a new way of seeing things.” - Henry Miller LAGM v. 2.023 LAGM2021
Edited by jay.ach (01/30/21 07:23 AM)
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bigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: PitcherCrab]
#27177515 - 01/30/21 07:35 AM (2 years, 11 months ago) |
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Quote:
PitcherCrab said: 1/29/2021
I finally have some really good looking mycelium to work with for both the TOC and TWC. I'll be transferring one more time then it's to grain!
Here are the plates I'm liking:

That myc looks good on all sectors of both varieties but I'm not liking that fuzz ball at 4 o'clock on the TOC plate
--------------------
I 5318008 NOT a virgin!
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GypsyBastard
Misanthrope


Registered: 11/30/20
Posts: 846
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Last seen: 2 years, 10 months
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: jay.ach]
#27177540 - 01/30/21 07:53 AM (2 years, 11 months ago) |
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I gave caps no prep WBS a go for my AA+ jars. I've used it before and don't remember having to tweak it in any way but these pt jars come out looking off; look\sound\feel way too dry when shaking. I just went with it but of the 3, I'm only seeing growth in 1. In 2, I can't even find the agar wedges or any signs that they ever were in the jar. So looking for some advice on prepless WBS. I will be PCing some MS(murashige&smooth, not multispore) today and would like to do some grain at the same time. My go to in the past was a 18-24 hour soak but I'm trying to get away from teks with large amounts of down time in the middle; want to be able to start and finish my cult work on the same day as there are not many days I have back to back available to work on this.
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The Dalcassian
Dirty Druggie



Registered: 01/04/17
Posts: 952
Loc: Emerald lsles
Last seen: 9 months, 3 days
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: GypsyBastard]
#27177578 - 01/30/21 08:20 AM (2 years, 11 months ago) |
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Not so Tat recovering from a shake. Its pretty slow on grain to be honest but it has been cold the last couple of weeks so maybe thats a factor.
-------------------- Here Lies This Individual 
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PitcherCrab
Crescent Fresh



Registered: 12/02/06
Posts: 2,446
Loc: The bottom of the sea.
Last seen: 1 year, 1 month
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Bigfoot, hahahaha I was wondering if someone would notice that! I dropped that plate, so that fuzz ball it the hunk of agar from that section of the plate. The mycelium I was trying to transfer came off the agar and stuck to the center. So it's not a contam, still TOC, just fuzzy.
-------------------- PC's LAGM 2021 (TOC & TWC)P. natalensis Growlog 2021Pans for PC Fall 2021 Growlog “I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear
Edited by PitcherCrab (01/30/21 08:29 AM)
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allthespores

Registered: 12/14/20
Posts: 42
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: LuxFlux]
#27177624 - 01/30/21 08:54 AM (2 years, 11 months ago) |
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Quote:
LuxFlux said: ... I have to kind of alter the teks a bit because I only have one hand that works right, which is also not helping much. Having two hands would make all this a LOT easier...
... It's going to be hard to work agar with sterile technique flame steralizing something, then keeping that clean while trying to open the petri dish with the same hand... it's why I lazied out and just did the alcohol but I can see that I really need to just work harder and figure it out.
I don't flame sterilize any of my tools for cultivation. I cover them with tinfoil and put them in the PC when ever I run it for jars or no pours 60-90 min.this way I can also use iso in the SAB and spray everything that goes inside. I open the bag with the tool in the SAB, making an sterile area where the tool can be rested shortly. Not saying its better then fire and you have to do more planing on what's needed for the situation.
-------------------- LAGM2021 Grow log
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Wall.E
Bacteria's Bitch



Registered: 06/05/20
Posts: 2,860
Loc: Fungal Void
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: allthespores]
#27177681 - 01/30/21 09:30 AM (2 years, 11 months ago) |
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I'll stick to my flame thanks
-------------------- Life’s shit, but I’m loving it
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ModularMind
M.P.F.



Registered: 02/09/10
Posts: 7,902
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: allthespores]
#27177690 - 01/30/21 09:36 AM (2 years, 11 months ago) |
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Quote:
allthespores said:
I don't flame sterilize any of my tools for cultivation.
If someone is conducting transfers of 12 different species, they may not want to sterilize/use 12 separate scalpels for that single session. A flame, for sterilization between plates/cultures, is pretty standard with agar work.
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D3_Myc
Weeb Trash



Registered: 05/06/18
Posts: 4,399
Loc: Year Zero
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Wall.E]
#27177693 - 01/30/21 09:39 AM (2 years, 11 months ago) |
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If I’m making prints I”ll bake all my tools but that’s not very often. I need to make one them fancy coil loop sterilizers though I love my blazer torch
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GypsyBastard
Misanthrope


Registered: 11/30/20
Posts: 846
Loc: The Mighty Boosh
Last seen: 2 years, 10 months
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: D3_Myc]
#27177707 - 01/30/21 09:49 AM (2 years, 11 months ago) |
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Induction coil FTW!!
though I have been too lazy to set my lab right lately so I'm just using my dab torch. Never had an issue with using ISO and a torch. I don't flame tools inside SAB as a torch running for even a few seconds in an enclosed space is going to create air currents. I can flame outside SAB and have the tool back inside while still glowing red so not exposed to open air except for while hot.
I do really miss using my coil though. Just something very satisfying about watching a blade glow red hot without actually touching anything.
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