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InvisibleJosex
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Wonko] * 2
    #27174949 - 01/28/21 05:06 PM (3 years, 4 hours ago)



If you never traded with filthy, do it! Hooks it up well beyond your wildest dreams. That being said I noc'd but a fraction that was in the filthy letter.


Also noc'd 4 lc's with the 3 things I was rocking for the LAGM.


Edited by Josex (01/29/21 02:07 PM)


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OfflineTattersail
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Josex]
    #27174983 - 01/28/21 05:31 PM (3 years, 3 hours ago)

Filthy's TOC and PESA have pinned on agar like no other I've had (in my fairly limited experience, but still!) and fruited brilliantly even though I've never cloned any of the cultures. I've got PESA on wbs at the minute and it's way ahead of all the other kinds of jars. Just munches everything up.


--------------------
LAGM2021
Trades

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OfflineBrownBear
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Tattersail]
    #27175010 - 01/28/21 05:48 PM (3 years, 3 hours ago)

I agree. TOC seems to pin readily on agar.  I love agar pins. They go directly into freshly sterilized lc's.


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OfflineGypsyBastard
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: BrownBear]
    #27175020 - 01/28/21 05:53 PM (3 years, 3 hours ago)

I have 2 PF Classic pins on agar that are giving me more pins already. I was trying to decide what to do with them. I suppose I could make up some LC to tomorrow.


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Invisiblemushhead
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Josex]
    #27175062 - 01/28/21 06:18 PM (3 years, 2 hours ago)

Quote:

Josex said:


If you never traded with filthy, do it! Hooks it up well beyond your wildest dreams. That being said I noc'd but a fraction that was in the filthy letter.


Also noc'd 3 lc's with the 3 things I was rocking for the LAGM.



You got the best of the best right there man I cannot wait to see how your tubs fair.
:teabird:


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OfflineGastronomicus
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: BrownBear]
    #27175134 - 01/28/21 07:06 PM (3 years, 2 hours ago)

I always just get exactly 1 agar pin. Like the myc puts all its hope into one lil mushie


--------------------
Make my Funk the P Funk, I wants to get Funked up

LAGM2024


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OfflineTankie_J
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Josex]
    #27175318 - 01/28/21 09:47 PM (2 years, 11 months ago)

Quote:

Josex said:


If you never traded with filthy, do it! Hooks it up well beyond your wildest dreams. That being said I noc'd but a fraction that was in the filthy letter.


Also noc'd 3 lc's with the 3 things I was rocking for the LAGM.




I was just tellin filthy how clean and aggressive that APEU is. You will not be disappointed Josex! And that Whytegold germs in like 2-3 days. :rockon:


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Offlinetiptrippy
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Tankie_J]
    #27175462 - 01/29/21 01:03 AM (2 years, 11 months ago)

Jan. 25 2021
 
The jar of pans didn't fully recover from the last shake which is pretty disappointing but I am so desperate to have a pan trip I decided to spawn it anyways.

 
x7x T3s turned into crap so I made up a fresh batch of agar and went back to the germ plate to grab some of these rhizo strands.

Jan. 28 2021
 
9g agar
6g lme
500ml distilled
This batch of agar and the rhizo strands from the grown out germ plate got me this sexy T1 plate.


Current growth on the Pans. It's looking pretty good so I have some peat/verm pasteurizing as I type this.

Updated OP here


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OfflineGypsyBastard
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: tiptrippy]
    #27175983 - 01/29/21 10:29 AM (2 years, 11 months ago)

This isn't strictly LAGM related but some of you may appreciate the idea.
Something I recently started doing (and really wish I had started at the beginning of my cult career) is creating slides. Slides of healthy myc from each variant, slides of spores during germination, slides of fungal and bacterial contams. Slides of shit that I don't know what it is. I've just started putting a small piece of anything I don't already have on a glass slide while I'm doing transfers and lab work.
A) it's just cool as hell to look at this stuff under a scope
B) helpful for identifying successful germination and\or potential contamination later on
C) even of I don't know what it is I'm looking at, I can tell if it looks different than what I want and avoid mistakes that may waste time.

I spent a lot of time looking at my LAGM plates under the scope but without having much of a frame of reference it's kind of just been for shits and giggles. Which is fine, but I would like for that to not be the case in the future.


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InvisibleDoctor Mario
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: GypsyBastard]
    #27175989 - 01/29/21 10:34 AM (2 years, 11 months ago)

I've been thinking about getting a scope but haven't done enough research on which ones are decent or the best magnification levels. Have a reccomendation?


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OfflineGypsyBastard
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Doctor Mario]
    #27176006 - 01/29/21 10:42 AM (2 years, 11 months ago)

I mean mine is definitely an El cheapo. Pretty sure it was like 180. The main things I looked for was dual lighting (above and below the slide), and a dimmer for the light. The 25x eye piece on the 4x lens is enough to see spores clearly. My biggest problem / complaint with this one, and I don't know that there is a remedy, is that the thickness of my Petri dishes doesn't allow me to use the higher power lenses. Which is in part where I started making the slides. For what I actually do with mine though, it does everything I need.


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OfflinePitcherCrab
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: GypsyBastard]
    #27176029 - 01/29/21 10:54 AM (2 years, 11 months ago)

Last time I looked into scopes I found that the AmScope B120 was the one to get. It comes highly recommended from a number of members here, and is a steal at $215!

Link: https://www.amscope.com/40x-2500x-led-binocular-compound-microscope-w-3d-two-layer-mechanical-stage.html?gclsrc=ds


--------------------
PC's LAGM 2021 (TOC & TWC)
P. natalensis Growlog 2021
Pans for PC Fall 2021 Growlog

“I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear



Edited by PitcherCrab (01/29/21 11:08 AM)


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Offlinelookintolearn
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: PitcherCrab]
    #27176071 - 01/29/21 11:16 AM (2 years, 11 months ago)

Finally got some decent looking growth on the PE plates, Just took T3 last night and probably gonna drop a bunch of these T2 to some grain to see what happens. Can't wait to start taking dick pics  :dancer:



Best looking plates so far:



Couple others that will be thrown on the grain just to see



--------------------
Don't be afraid of feeling the feelings

Lookin to LAGM 2021



Looking to start growing? Read through Bod's Introduction to Everything
Looking to start agar? Start with Alien's Holy Grail
Looking to perfect your transfers? Start with D3monic's Perfect Transfers
Looking for easiest prep to Coir ever? Eat's UNBUCKET Tek
Looking to start LC? Try LI first! Munch's super easy Blenderless LI


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OfflineLuxFlux
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: GypsyBastard]
    #27176074 - 01/29/21 11:18 AM (2 years, 11 months ago)

Quote:

GypsyBastard said:
Hey Luxflux, Why don't you walk us through your tek a bit more. I feel like I (and others too) may be misunderstanding what you're trying to do but if I am reading you correctly I'm afraid you might be heading for another let down. Coir isn't exactly a sub for BRF. What are you using for spawn? Are you using pre-made spawn bags? If you're looking to put spores on agar then you are definitely in the right place. Glad to hear you are motivated and learning as much as you can. The more info you give us, the better guidance you can get. And I can not stress enough how important it is the just follow the tek when starting out. Doesn't matter which one so much, just what ever one you pick, just follow the damn tek. Plenty of time to play around once you have fruits.




Purchased some MS syringes, injected into grain bags I purchased from a sponsor here with good reviews. One of the bags went modly within a week with a grey slimy mold- there was some mycelium but it got eaten/destroyed. I didn't have a SAB (just bought mine yesterday and haven't cut holes in it) but I cleaned the injection port with alcohol before I injected them. The needles were right from the autoclave bags so I went quickly, but didn't flame steralize- I think I will next time.

The bags were full of milo, I think- small, round grains.

The CVG I pulled from a tek on here. A handful of gypsum per 650 brick of coir, with 2 litres of boiling water per brick. I prepped the cooler by scrubbing the hell out of it and then warming it up with boiling water before I put the bricks in it. I don't remember the exact amount of verm I put in but it was a little too little and there was a little too much water in the whole thing. I didn't have a spare bucket but did have the cooler, I can't find the link to the tek I used but I tried to follow it pretty closely, knowing that the biggest mistakes newbies make is trying to go off on their own.

It took a few days to cool down enough I could work it/break up the coir at the bottom and mix it in. I mixed it with the grain spawn in a 1 part spawn to 2 parts substrate ratio for most of my tubs.

My two strains are Golden Teacher which looks agressive and healthy as fuck and B+ which is a little more grey and a lot less... filamenty? Mycelial? It looks a lot less agressive than the GT.

My larger monotub I mixed it with more spawn:sub because it was the entire bag of GT so it was the entire bag of spawn to the substrate.

I thought I was doing it right with the spore syringe > grain > growing out the myc in the grain bag > mixing with bulk substrate in tubs.

I'm still struggling a little with all the acronyms and terminology. I hope I said it right.

I have the tubs, a new pressure cooker I need to learn how to use, agar, petri dishes, and today I was going to buy a big bag of oats at the feed store (and a bag of wood pellets to grow oysters in case my purchase history starts throwing red flags beause I'm both paranoid and freaking LOVE eating mushrooms) and some big wide mouthed jars so I can set up jars ready to innoc. I need to order more syringes but I'm worried about wasting my money with them beause it's below freezing at night out here, and I am worried about them freezing in the mail.

I also got a bunch of minute rice bags but there's a lot of conflicting information about that. I just figured that was a good way to not use all my spore syrige on one bag of grain spawn and reduce the risk of loosing all my genetics to contamination.

I was thinking I just needed to get a couple of nice mushrooms and then clone them on the agar and start cleaning them up, and then I won't have to worry about getting spore syringes in the mail anymore.

This has turned into a crazy long journal- sorry about that, this has been a weird and lonely journey to try to get myself the medicine I need. I am just so afraid that after this time, effort and money I'll have to start again and wait another two or three months before I even have another shot at a harvest.


--------------------
Master conamination grower. My real hobby is spending all my money on mycology supplies.


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OfflineGypsyBastard
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: LuxFlux]
    #27176109 - 01/29/21 11:43 AM (2 years, 11 months ago)

This is probably not what you want to hear but MSS to grain is kinda asking for contamination. Even the best spore syringe from the most reputable and trusted vendor is going to have the chance for contamination. It's inherent in how they are made. There are a lot of little steps in your description that suggest you shot yourself in the foot on this one. The best advice I can offer is pick a tek and stick with it. Don't change anything. If you are working with spores, your best bet is agar or PF tek. Agar will take time (though I highly recommend learning it sooner rather than later) but PF tek will give you fruits sooner.
Check out this thread, bookmark it, and use it a lot until you feel comfortable with whatever method you use.
Square One
Not flame sterilizing your needle is also likely big contributor to your results.
Though it can work, I highly recommend not using instant rice and instead buying some regular brown rice and grinding it in a coffee grinder if you cannot find brown rice flour. I currently have some pf cakes running using oatmeal. That works well too and will be better than instant rice.
I would not recommend starting grain jars until you have gotten some successful fruits. Again, you don't want to start grain with spores, you want culture.
If you're in a hurry to get fruits get some brown rice or some rolled oats, follow the PF tech, do your work in a SAB, flame your needle and all the other precautionary steps involved follow the PF tek to a T and you will have success. Well that's working, start working on your sterile tech with agar to get culture growing so you can go to green later. Don't take this the wrong way, but if you were in a hurry to get fruits the last thing you want to do is put your spores on grain.


Edited by GypsyBastard (01/29/21 11:45 AM)


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Offlineroarkell
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: LuxFlux]
    #27176121 - 01/29/21 11:51 AM (2 years, 11 months ago)

For starters, spore syring to grain is at best a crap shoot... I understand you're just getting started and all that but if you want to be successful in this hobby, its almost imperative to start on agar... don't get me started on the fucking rice bags... I understand they can work but that argument is virtually the same as  the argument for communism...

As far as mixing the spawn and sub, jumping straight from the crap shoot that is your spawn bag to a mono is pretty risky... I should ask, was the spawn bag fully colonized? If you have uncolonized grain in your spawn bag, you can almost guarantee its gonna contam...

Id really suggest the PF tek if you want to get a good start... you can grate cakes and spawn them to bulk with a much higher success rate... they also require a lot less from each syringe... and once fully colonized you can steal some myc to throw on agar before you mix it with your sub...

Hopefully some of that is helpful... I don't want to be discouraging but sometimes its better to rip the bandaid off quickly. I wish you many successes in the future!


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OfflineTankie_J
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: roarkell]
    #27176184 - 01/29/21 12:43 PM (2 years, 11 months ago)

Took two Maz fresh print T1’s, three WGxBlueMeanie T1’s, and two WGxTOC T1’s. OP updated. Lagging behind on updating the Whytegold but they are looking great. Gotta make more plates, I ran out and see more cross transfers that can be made.

Photo drop:


Maz fresh print

Whytegold x Blue Meanie

Whytegold x TOC


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Offlinefalconman
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Tankie_J]
    #27176241 - 01/29/21 01:19 PM (2 years, 11 months ago)

Is it too late to join in? I have some variety I started a few weeks ago and wanna document with the bois!


--------------------
If any of my posted info is incorrect or questionable,please DO correct me. I strive to always learn new or better ways and information




Grower off all things nature!
things currently growing I am proud of:
50+ trichocereus named cultivars
B. Caapi that has died and came back twice
30+ varieties of herbs, berries, beans, and vegetables
psilocybe cubensis


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OfflineA.k.aM
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Tankie_J]
    #27176247 - 01/29/21 01:22 PM (2 years, 11 months ago)

There’s at least one vendor who makes clean syringes. Anywhere with PE syringes/syringes with dense spore balls will prob be ok because they’re made with a machine that separates bacteria and mold spores from the cubes.


I did syringe to grain 20+ times when I started and never had a problem. I was expecting to lose most of them so I did end up with way more mushrooms than planned :lol:


I even made clean lc from them.


This isn’t to say it’s the best way to grow, agar is definitely necessary for anything more than a minor hobby, but spore to grain has a very high success rate if you get the right stuff.


--------------------
LAGM2020


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OfflineLuxFlux
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: roarkell]
    #27176273 - 01/29/21 01:40 PM (2 years, 11 months ago)

Quote:

GypsyBastard said:
This is probably not what you want to hear but MSS to grain is kinda asking for contamination. Even the best spore syringe from the most reputable and trusted vendor is going to have the chance for contamination. It's inherent in how they are made. There are a lot of little steps in your description that suggest you shot yourself in the foot on this one. The best advice I can offer is pick a tek and stick with it. Don't change anything. If you are working with spores, your best bet is agar or PF tek. Agar will take time (though I highly recommend learning it sooner rather than later) but PF tek will give you fruits sooner.
Check out this thread, bookmark it, and use it a lot until you feel comfortable with whatever method you use.
Square One
Not flame sterilizing your needle is also likely big contributor to your results.
Though it can work, I highly recommend not using instant rice and instead buying some regular brown rice and grinding it in a coffee grinder if you cannot find brown rice flour. I currently have some pf cakes running using oatmeal. That works well too and will be better than instant rice.
I would not recommend starting grain jars until you have gotten some successful fruits. Again, you don't want to start grain with spores, you want culture.
If you're in a hurry to get fruits get some brown rice or some rolled oats, follow the PF tech, do your work in a SAB, flame your needle and all the other precautionary steps involved follow the PF tek to a T and you will have success. Well that's working, start working on your sterile tech with agar to get culture growing so you can go to green later. Don't take this the wrong way, but if you were in a hurry to get fruits the last thing you want to do is put your spores on grain.




Yeah my first attempt was PF tek. Then I kept reading that it's high labor/low reward, how things were done in the 90's and there's much better ways of doing it now. I'd alreay used my syringes on the grain bags by the time I learned that you can use syringes directly on agar. I thought you had to have a tissue culture first, which is why my first plan was "get fresh mushrooms by any means possible so you can then do it right"

I spend my lunch breaks and about an hour a day on this site after work, have for over a month and I still feel like I'm getting hit with a fire hose of information. I'm going to read all those links!

That's why I posted. I wanted to stay super anonymus but that was just going to lead to screwing up over and over. It's smarter to ask for help. Thanks for replying to me.

I have to kind of alter the teks a bit because I only have one hand that works right, which is also not helping much. Having two hands would make all this a LOT easier. The little things you take for granted, right? There is a lot to choose from- I'm trying to mostly stick to Bod's teks since there's a lot of commentary there and only changing things for accessibility reasons/when I don't have certain supplies (like the cooler instead of the bucket).

Quote:

roarkell said:
For starters, spore syring to grain is at best a crap shoot... I understand you're just getting started and all that but if you want to be successful in this hobby, its almost imperative to start on agar... don't get me started on the fucking rice bags... I understand they can work but that argument is virtually the same as  the argument for communism...

As far as mixing the spawn and sub, jumping straight from the crap shoot that is your spawn bag to a mono is pretty risky... I should ask, was the spawn bag fully colonized? If you have uncolonized grain in your spawn bag, you can almost guarantee its gonna contam...

Id really suggest the PF tek if you want to get a good start... you can grate cakes and spawn them to bulk with a much higher success rate... they also require a lot less from each syringe... and once fully colonized you can steal some myc to throw on agar before you mix it with your sub...

Hopefully some of that is helpful... I don't want to be discouraging but sometimes its better to rip the bandaid off quickly. I wish you many successes in the future!




I appreciate the time to respond to me, brother. The first completely failed batch I did was PF tek, and when I was reading on different techniques I kept seeing that monotubs were better and the way to go.

I thought my grain bags were at 100%, they looked like the pictures of 100% grain bags on here- and then I waited an extra three days just to be sure :smile: It felt like threading a needle between under colonized and the mycelium getting weak from having waited too long.

I mean right now the articles and threads I'm reading here seem to point towards agar, liquid culture and slurry. It's just a LOT of information for someone new to absorb and I don't know enough or have enough actual experience to do a quality filter for what I'm reading. Like, I don't know what's a good harvest weight for different sized boxes so if a tek is like "I got 12 grams dried from a shoebox over 3 flushes" I don't know if that's good, bad, or total bullshit/impossible lies.

I'm trying not to be too identifying but I only have one working hand not amuptated, that'd be way too identifying- I can kinda/sorta/sometimes use both hands but not really, if that makes sense. Any advice on that is DEEPLY appreciated.

The Uncle Ben's tek looked a little easier for someone to do one handed. It's going to be hard to work agar with sterile technique flame steralizing something, then keeping that clean while trying to open the petri dish with the same hand... it's why I lazied out and just did the alcohol but I can see that I really need to just work harder and figure it out.

I appreciate the advice, I'd rather have the truth even if it means I gotta start over than struggle in ignorance.

I'm cutting the holes in my SAB tonight and hope I'll get some pasty plates and oats tonight. I wanted to try no pour agar in the pressure cooker while I made the pour agar and practiced on the petri dishes to see if I can do them, and then wait a week or two and see if they start growing shit that shows my sterile technique just isn't up to snuff. :grin:

I figure that'll be less frustrating that either waiting for more spores or making a bunch of contaminated plates.


--------------------
Master conamination grower. My real hobby is spending all my money on mycology supplies.


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