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OfflineGypsyBastard
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: GypsyBastard]
    #27171739 - 01/26/21 08:18 PM (3 years, 2 days ago)

And immediately after posting that I went to move all the plates, dropped one of the stacks and half of the transfers I just did are not stuck to the sides and top of the plates. I fucking hate myself right now.


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OfflineLuxFlux
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: D3_Myc]
    #27171755 - 01/26/21 08:23 PM (3 years, 2 days ago)

My last attempt at cultivation was a huge disaster and a joke. Let's just say I spent a fair amount of money to cultivate a beautiful, thick, healthy batch of trich.

Eeew.

The jars were so gross and smelled so bad I just threw them out- it wasn't worth it to me to open them up, get mold spores in my house, and deal with the smell. I just bought more jars. I did seem to do everything a la 1998 tek and do it wrong as well. My syringes likely froze in the mail,

This time I just spend a certain portion of my cash every paycheck to get things set up right. I have a pressure cooker, coir instead or brf and shredded cardboard, I got two sets of syringes and commercial spawn bags.

I still need to make a run to get shoeboxes, monotubs and pasty plates and I need a SAB but I have agar, petri dishes, and two spawn bags that managed to inoculate.

How much did I screw it up?

2/3 made it. One was contamed and went grey and slimy instantly, so I threw it out. It's been over a month and a half for the bags to get to the point the look fully colonized! My house, apparently, is too damn cold.

I've got a cooler full of Bod's bucket tek style coir soaking in boiling water, with two added handfulls of gypsum and some vermiculite.

I've been spending my lunches and time after work every day here reading, and I hope that I'll be able to actualy grow a mushroom or two.

Thank you everyone for sharing your knowledge so freely! I'm excited to join the ranks of amature mycologists.


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Master conamination grower. My real hobby is spending all my money on mycology supplies.


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OfflineGastronomicus
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: LuxFlux]
    #27171790 - 01/26/21 08:42 PM (3 years, 2 days ago)

Are you going spore syringe to coir? Don't do that


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Make my Funk the P Funk, I wants to get Funked up

LAGM2024


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OfflinePitcherCrab
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Gastronomicus]
    #27171802 - 01/26/21 08:46 PM (3 years, 2 days ago)

Sounds like he's going spores to grain bags to coir.


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PC's LAGM 2021 (TOC & TWC)
P. natalensis Growlog 2021
Pans for PC Fall 2021 Growlog

“I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear



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InvisibleThe Fresh PrintsS
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: PitcherCrab]
    #27171829 - 01/26/21 08:54 PM (3 years, 2 days ago)

Quote:

01/26/21


Gonna send both of these TWC this weekend


And I'll probably send 5 of these melmac this weekend as well.





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OfflineGypsyBastard
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: PitcherCrab]
    #27171830 - 01/26/21 08:55 PM (3 years, 2 days ago)

Hey Luxflux, Why don't you walk us through your tek a bit more. I feel like I (and others too) may be misunderstanding what you're trying to do but if I am reading you correctly I'm afraid you might be heading for another let down. Coir isn't exactly a sub for BRF. What are you using for spawn? Are you using pre-made spawn bags? If you're looking to put spores on agar then you are definitely in the right place. Glad to hear you are motivated and learning as much as you can. The more info you give us, the better guidance you can get. And I can not stress enough how important it is the just follow the tek when starting out. Doesn't matter which one so much, just what ever one you pick, just follow the damn tek. Plenty of time to play around once you have fruits.


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InvisibleWall.E
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: GypsyBastard]
    #27171832 - 01/26/21 08:55 PM (3 years, 2 days ago)

Quote:

Myc_Hunt said:
1/26/2021

Got some T2's going to T3 and LC. Also put 2 natalensis colonies on grain to see if they will produce different results based off their mycelial structures

Jambos


Natalensis





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Life’s shit, but I’m loving it



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OfflineGypsyBastard
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Wall.E]
    #27171842 - 01/26/21 09:03 PM (3 years, 2 days ago)

update.2
A bit out of order and context as I don't remember which pic are from which plates but....... YAY MYCROSCOPY!!!!


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OfflineGrinchGrower
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: GrinchGrower]
    #27171845 - 01/26/21 09:05 PM (3 years, 2 days ago)

Quote:

GrinchGrower said:
- 01/26/21: T-3




Took five transfers from some select plates
____________






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Invisiblecapntrips7
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Registered: 10/21/20
Posts: 91
Loc: Urithiru
Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: GypsyBastard]
    #27171925 - 01/26/21 10:06 PM (3 years, 1 day ago)

Quote:

GypsyBastard said:
And immediately after posting that I went to move all the plates, dropped one of the stacks and half of the transfers I just did are not stuck to the sides and top of the plates. I fucking hate myself right now.




Welcome to the club. Unsurprisingly, the one I dropped to the floor of the SAB is the worst performer of the bunch:



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LAGM2021


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InvisibleDoctor Mario
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: capntrips7]
    #27171928 - 01/26/21 10:09 PM (3 years, 1 day ago)

I don't wanna be that guy but that looks like mold. Maybe its just the picture quality but I don't like how disorganized the growth on that is.


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Invisiblebigfootscreepyuncl
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: bigfootscreepyuncl]
    #27171932 - 01/26/21 10:10 PM (3 years, 1 day ago)

Quote:

1/26/21 After wasting a sleeve or two of plates that wouldn't germinate I said fuck it and made a batch of BRF pucks. This is LAGM and desperate times call for desperate measures! My GT and PE finally got off their lazy asses and germinated. I took transfers from the pucks to agar on 1/22/21 and just made the T-2 tonight.

These are the T-1s from the pucks:

GT

PE

PE I was awfully tempted to send this one to LC but decided I'd better at least make another transfer. My hands got shaky as fuck when taking these transfers so hopefully I didn't jack this one up.





I've been doin' a shit job keeping my post updated but I haven't had a lot of successes to share or time to get much cult work in, but here's what I've got for now. I'm really proud of that 3rd picture of the PE plate. That's my most rhizo growth yet and only a T-1 so my hopes are high! Probably shoulda just sent it to LC..oh well

This update was a pretty rushed so in the next day or two I'll give better updates and clean up the last edit


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I :heart: 5318008


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InvisibleDoctor Mario
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: bigfootscreepyuncl]
    #27171943 - 01/26/21 10:16 PM (3 years, 1 day ago)

I try not to get too caught up in the rhizo chase but damn they are beautiful. Good job.


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OfflineTankie_J
I like having fun
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Registered: 01/09/19
Posts: 537
Last seen: 10 months, 13 days
Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: bigfootscreepyuncl]
    #27171959 - 01/26/21 10:28 PM (3 years, 1 day ago)

Hey Bigfoot, how did you go about transferring from the puck? What tools or method of getting the myc out successfully? I have germination on a pan cyan puck but it looks like there is a spot of mold in there as well. I want to get it out asap and in as quick and fluid motion as possible.


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Invisiblecapntrips7
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Registered: 10/21/20
Posts: 91
Loc: Urithiru
Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Doctor Mario]
    #27171979 - 01/26/21 10:46 PM (3 years, 1 day ago)

Quote:

Doctor Mario said:
I don't wanna be that guy but that looks like mold. Maybe its just the picture quality but I don't like how disorganized the growth on that is.




I agree with you. None of my GT plates look good. It was my first attempt at going from a spore print, and I just lightly dragged my flimsy wire loop around the very light print. I went back to spores with a dark print that I scraped clean with the back of a scalpel blade, so I'll hopefully have better luck this go round.



LAGM2021 Update

Log updated:

Quote:

capntrips7 said:

Day 23, 2021-01-25
Prepped agar and LC

Day 24, 2021-01-26
Prepped spores, inoculated plates, and made transfers




I did a poor job of keeping up with isolates in my T2 plates, so I made a fresh round:



I also dropped a puck to LC, my first such attempt. I kinda winged it based on D3's comments from earlier in the thread.

The T2 plates are looking decent, but I imagine I'll only hold onto them in case something happens to the new ones:



None of my GT plates were looking all that exciting:



So I went back to spores with one of my latest prints, my first attempt at making a spore syringe from a print. I used 1% agar, since I've seen several people say wetter is better for germination plates. I also took this opportunity to try to make up spore syringes from an APE swab and RW print (Thanks roar!). I used a very simplified version of faht's tek. I also cut the leftover swab in half and swiped it on a pair of plates.

GypsyBastard, If you get down this far, my listing should now be:

Species: P. Cubensis
Variant: B+, APE, RW, GT, AA+
Species: P. Tampanensis

Thanks.

My Tamp jar and shoebox both seem to have stalled out, so I'm starting to think I'm growing mold. I went back to the (contaminated) germ plate in hopes of recovering something, so we'll see if anything happens there at some point in the future. I've got a few fruits in my AA+ jar, so I might have at least a tiny spore print in the next few days.


--------------------
LAGM2021


Edited by capntrips7 (01/27/21 01:20 AM)


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OfflinePitcherCrab
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: capntrips7]
    #27172040 - 01/26/21 11:59 PM (3 years, 1 day ago)

1/26/2021

I made a few more transfers today. My TWC and TOC T2 plates are looking really good so I decided to make a few more transfers from them.

TWC & TOC T2's:


I had already made T3 transfers, but for whatever reason they weren't giving me the kind of growth I was after. I made a T4 of the TWC because there was some good growth one of the sections of my T3 plate, but I didn't bother with the TOC because nothing was looking that good to me. I think the new transfers I got from the T2's should more than make up for it.

TWC & TOC T3's, not too much to work with here:


I also have a few plates of exotics I'm excited about I thought I'd throw in here for kicks. Here are an Atl#7, P. tamp. Pollock and P. subtropicalis looking good. I'm excited to get these guys onto grain after a couple more transfers.



--------------------
PC's LAGM 2021 (TOC & TWC)
P. natalensis Growlog 2021
Pans for PC Fall 2021 Growlog

“I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear



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Invisibleallthespores

Registered: 12/14/20
Posts: 42
Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: tiptrippy]
    #27172232 - 01/27/21 04:50 AM (3 years, 1 day ago)

Quote:

tiptrippy said:

I bring enough water to cover the WBS plus an inch or two to a rolling boil in my PC then turn off the heat and move PC to another burner and immediately dump WBS into the PC and put the lid on locked. Let that sit over night, dump water and strain for 15 mins, load into jars and PC and they come out perfect every time. Maybe a little wet still after PC cycle but shake them every now and then while cooling and all the moisture gets distributed evenly.




thank you for sharing, i really liked the look of that panaeolus wbs jar of yours. will follow this tek.


--------------------
LAGM2021 Grow log


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Invisiblebigfootscreepyuncl
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Tankie_J]
    #27172335 - 01/27/21 07:23 AM (3 years, 1 day ago)

Quote:

Tankie_J said:
Hey Bigfoot, how did you go about transferring from the puck?




Awkwardly and shakily as hell lol. It was really an awkward process for me. But, with that being said I just used my scalpel (Xacto knife) and just kinda scraped it into a clump and tried lifting it out :shrug: I'm amazed it's as clean as it is on agar.

I ended up having to transfer a small clump of the puck with it on the PE plates, but the GT was really fluffy myc so I just kinda scooped it with the blade and rolled it into a mini ball kinda thing until I could lift it out. It worked pretty well.

That probably isn't much help but that's what I did lol. Best of luck to ya!


--------------------



I :heart: 5318008


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OfflineSandwichMan
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: roarkell]
    #27172349 - 01/27/21 07:34 AM (3 years, 1 day ago)

What kind of Gyms if you don’t mind my asking.


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Cult Pronunciations

Muda's Bottle Tek

Kizzle's Contam Guide

Bod's Cult Glossary

I just play a character in this fantasy land.


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OfflineBallzagna
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: bigfootscreepyuncl]
    #27172461 - 01/27/21 08:50 AM (3 years, 1 day ago)

Quote:

bigfootscreepyuncl said:
Quote:

Tankie_J said:
Hey Bigfoot, how did you go about transferring from the puck?




Awkwardly and shakily as hell lol. It was really an awkward process for me. But, with that being said I just used my scalpel (Xacto knife) and just kinda scraped it into a clump and tried lifting it out :shrug: I'm amazed it's as clean as it is on agar.

I ended up having to transfer a small clump of the puck with it on the PE plates, but the GT was really fluffy myc so I just kinda scooped it with the blade and rolled it into a mini ball kinda thing until I could lift it out. It worked pretty well.

That probably isn't much help but that's what I did lol. Best of luck to ya!




"so yeah you just use the scalpel and get a piece out" :rofl:

Couldn't have said it better myself tbh


--------------------
No one likes a naysayer

It's probably fine. Even if it's not fine, it'll eventually be fine. So it's fine.
PE Adventure
:sunny:Photosynthesis: A Morning Ritual:sunny:
Ballzagna's Tek Compendium and Notes for Noobs




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