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InvisibleDoctor Mario
πŸ„πŸ„πŸ„πŸ„πŸ„
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Registered: 08/07/20
Posts: 1,894
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Narrator]
    #27158384 - 01/19/21 09:27 PM (3 years, 8 days ago)

1-19-2021
APEU T1


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OfflinePitcherCrab
Crescent Fresh
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Registered: 12/02/06
Posts: 2,446
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Doctor Mario]
    #27158531 - 01/19/21 11:37 PM (3 years, 8 days ago)

1/19/2021

I'm really liking how this T2 TWC is coming along. Nice and fast for 2 days of growth. The TOC is a little behind but also doing well. I decided not to include it in this update because there was too much condensation on the plate to get a good shot. Perhaps I'll have better luck tomorrow.



One more update, the TOC LC I started with spore was contaminated with spiderweb mold, I found out after putting some to grain. That'll be the last time I do that! :facepalm:

OP updated here: https://www.shroomery.org/forums/showflat.php/Number/27125101#27125101


--------------------
PC's LAGM 2021 (TOC & TWC)
P. natalensis Growlog 2021
Pans for PC Fall 2021 Growlog

β€œI must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear



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Offlinetiptrippy
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: PitcherCrab]
    #27158591 - 01/20/21 12:50 AM (3 years, 8 days ago)

Jan. 19 2021

 

Gave it one last shake. I'll mix up some poo/straw tomorrow and pasteurize.


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OfflineA.k.aM
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: tiptrippy]
    #27158596 - 01/20/21 12:52 AM (3 years, 8 days ago)

I wish I could fast forward a month when this’ll be chock full of fruiting pictures.


Finally just got around to doing t1s myself.


--------------------
LAGM2020


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OfflineTankie_J
I like having fun
I'm a teapot


Registered: 01/09/19
Posts: 537
Last seen: 10 months, 13 days
Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: PitcherCrab]
    #27158597 - 01/20/21 12:53 AM (3 years, 8 days ago)

Don’t check in for a day and stuff is just blowing up! All yous guys is killing it! I have T1’s happening tomorrow on APEU and Whytegold. There is def germ on a pan cyan plate from 3JAN that will be transferred in a few days. Will update op accordingly.


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OfflineFailboat
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Tankie_J]
    #27158632 - 01/20/21 01:33 AM (3 years, 8 days ago)

I've made T1 of TWC. No RW growth it seems, but some of the TWC spores I streaked with RW to cross jave germinated. :shrug:


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OfflineMycanthrope
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Registered: 01/19/21
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Failboat]
    #27158723 - 01/20/21 03:47 AM (3 years, 8 days ago)

Newbie grower here throwing my hat in the ring! This'll be my first time trying agar and I'm nervous because the only spores I've got is from my first pf tek grow a few years back and I did not know how to take prints. They're on paper and I know the surface the paper was on molded under at least one of the prints at some point... but might as well give it a shot. Transfers solve everything? I do have a grow in progress, but I'm not sure when I'll get the spores from that.

Mycanthrope Grow Log

Species: P. Cubensis
Variant: B+ Melmac, x7x, Gulf Coast, Golden Halo

Plate recipe: MEA

1/20:

Pressure cooked Agar
Poured Agar
Loop inoculated with B+ spores x 6

Forgive the ketchup cup, I've got properly sterilizable containers coming in soon.



1/21:

5 of the 6 I inoculated are growing something!



1/23:

Welp, looks like all I'm germinating is mold :/ kinda a blue/grey with white edges. Every plate looks identical so the print has to be bunk.




1/30:

Thanks to the generous donation of Melmac swabs from Quirk, I put 3 plates of melmac down.

2/9:

Roar's help means that I've got a few more strains to play with, and I'm also ready for attempting clones on my malabar grow (not lagm), so today was a massive SAB day resulting in 13 plates used up!

I made 3 transfers from my melmac plates.


I also made 2 plates each of x7x, Gulf Coast, and Golden Halo.


Edited by Mycanthrope (02/11/21 10:33 AM)


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Offlineroarkell
The Roar Master!
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Registered: 08/29/19
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Mycanthrope]
    #27158728 - 01/20/21 03:59 AM (3 years, 8 days ago)

Quote:

Mycanthrope said:
Newbie grower here throwing my hat in the ring! This'll be my first time trying agar and I'm nervous because the only spores I've got is from my first pf tek grow a few years back and I did not know how to take prints. They're on paper and I know the surface the paper was on molded under at least one of the prints at some point... but might as well give it a shot. Transfers solve everything? I do have a grow in progress, but I'm not sure when I'll get the spores from that.

Mycanthrope Grow Log

Species: P. Cubensis
Variant: B+

Plate recipe: MEA

1/20:

Pressure cooked Agar
Poured Agar
Loop inoculated with B+ spores x 6

Forgive the ketchup cup, I've got properly sterilizable containers coming in soon.





We all had to start somewhere! Lol... if you can't get anything going from your paper print, shoot me a message and I'll get ya some good, clean prints!


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OfflineBallzagna
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: roarkell]
    #27158968 - 01/20/21 08:29 AM (3 years, 8 days ago)

Quote:

roarkell said:
Quote:

Mycanthrope said:
Newbie grower here throwing my hat in the ring! This'll be my first time trying agar and I'm nervous because the only spores I've got is from my first pf tek grow a few years back and I did not know how to take prints. They're on paper and I know the surface the paper was on molded under at least one of the prints at some point... but might as well give it a shot. Transfers solve everything? I do have a grow in progress, but I'm not sure when I'll get the spores from that.

Mycanthrope Grow Log

Species: P. Cubensis
Variant: B+

Plate recipe: MEA

1/20:

Pressure cooked Agar
Poured Agar
Loop inoculated with B+ spores x 6

Forgive the ketchup cup, I've got properly sterilizable containers coming in soon.





We all had to start somewhere! Lol... if you can't get anything going from your paper print, shoot me a message and I'll get ya some good, clean prints!






made this meme just for you Roar


--------------------
No one likes a naysayer

It's probably fine. Even if it's not fine, it'll eventually be fine. So it's fine.
PE Adventure
:sunny:Photosynthesis: A Morning Ritual:sunny:
Ballzagna's Tek Compendium and Notes for Noobs




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Offlineroarkell
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Ballzagna]
    #27158987 - 01/20/21 08:40 AM (3 years, 8 days ago)

Quote:

Ballzagna said:
Quote:

roarkell said:
Quote:

Mycanthrope said:
Newbie grower here throwing my hat in the ring! This'll be my first time trying agar and I'm nervous because the only spores I've got is from my first pf tek grow a few years back and I did not know how to take prints. They're on paper and I know the surface the paper was on molded under at least one of the prints at some point... but might as well give it a shot. Transfers solve everything? I do have a grow in progress, but I'm not sure when I'll get the spores from that.

Mycanthrope Grow Log

Species: P. Cubensis
Variant: B+

Plate recipe: MEA

1/20:

Pressure cooked Agar
Poured Agar
Loop inoculated with B+ spores x 6

Forgive the ketchup cup, I've got properly sterilizable containers coming in soon.





We all had to start somewhere! Lol... if you can't get anything going from your paper print, shoot me a message and I'll get ya some good, clean prints!






made this meme just for you Roar



Haha! I might have to steal that for my sig! Lol


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OfflineBallzagna
Carb Daddy
Male


Registered: 10/09/19
Posts: 619
Last seen: 13 days, 10 hours
Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: roarkell]
    #27158996 - 01/20/21 08:44 AM (3 years, 8 days ago)

all you buddy :thumbup:


--------------------
No one likes a naysayer

It's probably fine. Even if it's not fine, it'll eventually be fine. So it's fine.
PE Adventure
:sunny:Photosynthesis: A Morning Ritual:sunny:
Ballzagna's Tek Compendium and Notes for Noobs




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Offlineredbee
prole spore threat
Female

Registered: 03/30/20
Posts: 175
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Ballzagna]
    #27159231 - 01/20/21 10:30 AM (3 years, 8 days ago)

Updated the log with photos of my T1's of RW/APEU. Truckin' along... :robindance:


Quote:

redbee said:

JANUARY 20, 2021


Took T1's from the spore plates last Friday. All three are looking clean. I'll pick the two best from each set and transfer those again in a couple of days.


APEU:



APEU looking fine. Any thoughts on the one with the double-ringed myc situation? Normal? Looks spooky.


Rusty Whyte:



Close-up on the most rhizo-looking RW plate I've ever seen :lol:
Both of the two bottom plates are from the same source plate, and I'll be taking those to T2.







--------------------
:sporedrop:  redbee's LAGM 2021  :sporedrop:


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InvisibleJosex
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: redbee]
    #27159262 - 01/20/21 10:42 AM (3 years, 8 days ago)

Nice stuff, redbee! Can I ask you where you sourced those deli cups? They look so nice and visibility is awesome. People who want to try my no-pour tek often find that it's not easy to source adequate containers, especially people in the US.


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OfflineGypsyBastard
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Josex]
    #27159278 - 01/20/21 10:48 AM (3 years, 8 days ago)

@ Mycanthrope- Got you added in my friend. Welcome aboard.

@ redbee- Same question as Josex. Those look like they would be great for some in vitro seed germination I'm doing.




While updating main page with new member, I realized there are a lot of people MIA in this thread. To be expected I suppose and hopefully they will turn up eventually with some tubs full of fruits.


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OfflineOne of Us
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: GypsyBastard]
    #27159311 - 01/20/21 11:10 AM (3 years, 8 days ago)

Hey gypsy, does the invitro seed germination involve orchids?

Ever since I found out they are germinated in agar, I've been obsessed with trying my hand at it, but it seems no body sells seeds.


--------------------
LAGM 2021
LAGM 2020
Trade List


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Invisiblemushhead
Potato Devourer
I'm a teapot


Registered: 08/22/14
Posts: 2,215
Loc: Dimension X-124
Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: GypsyBastard]
    #27159337 - 01/20/21 11:23 AM (3 years, 8 days ago)

Quote:

GypsyBastard said:
@ Mycanthrope- Got you added in my friend. Welcome aboard.

@ redbee- Same question as Josex. Those look like they would be great for some in vitro seed germination I'm doing.




While updating main page with new member, I realized there are a lot of people MIA in this thread. To be expected I suppose and hopefully they will turn up eventually with some tubs full of fruits.



You can always count on me to be around annoying people.


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OfflineGypsyBastard
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: mushhead]
    #27159360 - 01/20/21 11:33 AM (3 years, 8 days ago)

Quote:

One of Us said:
Hey gypsy, does the invitro seed germination involve orchids?

Ever since I found out they are germinated in agar, I've been obsessed with trying my hand at it, but it seems no body sells seeds.




Nah, they're Trichocereus seeds. Though I am considering trying to germ some poppy seeds that way as well.

Quote:

mushhead said:
You can always count on me to be around annoying people.




Ha, I almost added a sentence to that post about the 5-10 of us on here that never seem to shut up (myself included) but didn't want that to come off the wrong way. Totally mean it in the best way possible. You guys definitely help me keep my mind busy. :smile:


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Offlineredbee
prole spore threat
Female

Registered: 03/30/20
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Josex]
    #27159361 - 01/20/21 11:33 AM (3 years, 8 days ago)

Quote:

Josex said:
Nice stuff, redbee! Can I ask you where you sourced those deli cups? They look so nice and visibility is awesome. People who want to try my no-pour tek often find that it's not easy to source adequate containers, especially people in the US.




Here's those deli-style containers:
Augshy Containers 50 Pack

And, this is the best deal on the "holy grail" style dishes that I've seen in a while... 20 for $12, instead of 8 for $14:
Amersumer 20 Pack Containers


Josex, I've read through your deli-cup tek, and it definitely helped me figure out how to work with them, so thanks for that! I differ from you in one time-saving way, though. I don't fuss with labeling or matching the lids. I found that pouring warm agar will (obviously) heat the cups, so that when I go to attach the (non-heated) lids a few minutes after pouring, there's always a bit of trouble getting them on. Cups have ever-so-slightly temporarily deformed from the heat of the agar, clearly.

Solution? I wait 20 minutes for the agar in the cups to totally cool to room temp, and then the lids fit just fine.

I've gone through... I donno... half a dozen PC cycles with these, and I still have no issues with lid/cup matching if I just wait a while after pouring the agar.


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OfflineGypsyBastard
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: redbee]
    #27159384 - 01/20/21 11:43 AM (3 years, 8 days ago)

Oh shit that's what those are?!?! They looked different in the pics for some reason. Fuck I can get a case of those for free. Kinda mad at myself for not thinking of those sooner. Hopefully my MS agar will be in this weekend and I can grab some of those. Want to get these things going some they can spend later summer and fall out in the sun. Thanks for the reply and info and inspiration!!


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Invisiblemushhead
Potato Devourer
I'm a teapot


Registered: 08/22/14
Posts: 2,215
Loc: Dimension X-124
Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: GypsyBastard]
    #27159387 - 01/20/21 11:45 AM (3 years, 8 days ago)

Quote:

GypsyBastard said:

Quote:

mushhead said:
You can always count on me to be around annoying people.




Ha, I almost added a sentence to that post about the 5-10 of us on here that never seem to shut up (myself included) but didn't want that to come off the wrong way. Totally mean it in the best way possible. You guys definitely help me keep my mind busy. :smile:



I'm just extremely passionate about mushrooms, you've no idea the amount of literature I've consumed this year alone on the subject.


--------------------
Meditation Principles

Silence: Giving you room to listen / Stillness: Giving you room to feel / Spaciousness: Just giving you room


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