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tiptrippy
The Mechanic



Registered: 09/09/20
Posts: 1,131
Loc: United States
Last seen: 1 year, 6 months
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: A.k.a]
#27155553 - 01/18/21 02:01 PM (3 years, 10 days ago) |
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Jan. 11 2021
I mentioned doing a LC in my last update. I will only do that for the x7x. I only need 1qt of spawn at a time for the Pan Cyans.
- Dropped a large wedge of Pan Cyan to 1qt grain.
- Took 7 or 8 xfers of x7x each to their own plate.
Jan. 12 2021

Very fast growth on my Pan Cyan culture.
Jan. 18 2021

This is current growth 2 days after the jar was shaken on Jan 16. I already have a tray of Pans going in my FC so I'm not sure what I'm going to do with this jar yet. I didn't anticipate such fast growth.
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Ambrose

Registered: 01/16/20
Posts: 518
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: tiptrippy]
#27155921 - 01/18/21 05:00 PM (3 years, 10 days ago) |
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I thought the above two Chicon T1 plates looked good enough so decided to send them to grain yesterday. I took a transfer from both in case it doesn't work out. Here's a jar one day later:

I fucked up the prep, first time using WBS, think I made it too wet. On top of that the jars I've never used before, they're pasta sauce jars. The lids seemed decent though so hopefully no problems(so far actually better at least ergonomically than regular 2 piece mason jar lids.)
The two Jalisco T1's are moving along(albiet slowly):


The first one if it continues to look good I'll send to grain, the second isn't uniform so I'm gonna take another transfer before then.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,274
Loc: where?
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Ambrose]
#27156207 - 01/18/21 07:52 PM (3 years, 10 days ago) |
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finally took some transfers. the myc indeed is clean so i took a lot
 
just the PE. cbk aa+ aint doin shit
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Mr. Mushie

Registered: 01/16/20
Posts: 667
Last seen: 2 months, 24 days
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: mushhead]
#27156218 - 01/18/21 07:58 PM (3 years, 10 days ago) |
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Quote:
mushhead said: Jan the 12th.
 Update 1/18/21 Looks like the myc has finally taken ahold of the grain. Woot woot chicken foot.
Oh boy these grains look nice. No burst grains... link me to your methods.
Is this Rye?
I've been rocking it lately but always get a few burst when it's simmering. I'd rather that than a handful of under hydrated grains slip by and stall some jars.
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Jarhead3521
Grindin’



Registered: 09/26/19
Posts: 514
Loc: Amongst the pines
Last seen: 5 months, 22 days
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Mr. Mushie]
#27156752 - 01/19/21 04:57 AM (3 years, 9 days ago) |
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1/18/2021: Not much of an update, small germ on a LAGM21 PE plate, so I took 3 punches. The Malabar and huatla aren’t doing shit, BUT I did resurrect an old FK PESA, one of my first received, and finished it out on 4 germ plates, a GT that I printed last year and swiped a swab from Fahtster. Put a couple plates to grain and made transfers off couple ESS germ plates I swiped the swab from brownbear on.
--------------------
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capntrips7
Moldsmith

Registered: 10/21/20
Posts: 91
Loc: Urithiru
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Mr. Mushie]
#27156754 - 01/19/21 05:07 AM (3 years, 9 days ago) |
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After 6 days, I've got some faint white growth on my GT plate:

This was my first time inoculating from a spore print. It was a very light print from my first grow, and I may not have been aggressive enough at scraping it with the inoculation loop.
I put a few drops of distilled water on the print, lightly ran the loop around the print a few times, then used c10's streak tek.
I did not make a special batch of agar. I just used existing plates using the recipe from d3's agar tek.
My only previous experience is with spore syringes, and the growth has been a bit thicker, so I'm not sure if I'm looking at mold or mycellium. What do you guys think, wait it out a few more days or streak another plate?
If I need to streak a new plate, got any suggestions on improving my chances? I've seen several people mention making a wetter agar by decreasing the percentage of agar agar powder. Should I also adjust the nutrients? scrape the print with a scalpel before streaking the plate?
-------------------- LAGM2021
Edited by capntrips7 (01/19/21 05:25 AM)
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bigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: capntrips7]
#27156763 - 01/19/21 05:16 AM (3 years, 9 days ago) |
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That looks like proper myc to me, though a bit wispy. So far that's what my germ plate APE, PE and (to a lesser extent) Chitwan have looked like, though. I think you're in good shape. Take some transfers and I'm guessing it will shape up for you.
As far as agar recipes go, I've been seeing lately people are altering their germination plate recipes opposite how I would expect: ~7.5-8g agar:11.5g LME:500ml. I would have thought lowering the nutrients as well as agar would be best but I've seen this referenced several times (never used it myself, mind you).
I just did D3monics TEK but used 9g agar and 8g LME for transfers. I'm getting awesome rhizo growth on my T-2s, APE is absolutely crawling on it (to be expected). Cheers, mate!
--------------------
I 5318008 NOT a virgin!
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capntrips7
Moldsmith

Registered: 10/21/20
Posts: 91
Loc: Urithiru
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Thanks for the reply. I'll make a few transfers, see what happens.
-------------------- LAGM2021
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Doctor Mario
🍄🍄🍄🍄🍄



Registered: 08/07/20
Posts: 1,894
Loc: 🇺🇸
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: capntrips7]
#27156804 - 01/19/21 05:51 AM (3 years, 9 days ago) |
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Mycelium always looks suspect to me when it germinates. It's not really sure what the hell is going on or what it wants to do in life.
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filthyknees
no coincidence


Registered: 03/08/13
Posts: 6,283
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Doctor Mario]
#27156935 - 01/19/21 07:36 AM (3 years, 9 days ago) |
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-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
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capntrips7
Moldsmith

Registered: 10/21/20
Posts: 91
Loc: Urithiru
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: filthyknees]
#27156978 - 01/19/21 08:06 AM (3 years, 9 days ago) |
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Log updated:
Quote:
capntrips7 said:
Day 17, 2021-01-19
Made transfers to plates 13-15.

The growth was a bit whispy, but hopefully it will do better on the T1 plates. I transferred it to 3 different configurations: 1% BRF, 1% LME, and 1.5% BRF.
Unfortunately, I dropped the 1.5% BRF plate while wrapping, and the puck got stuck to the lid. When I tried to fix it, it fell to the floor of the SAB, so I expect it will immediately go contam.
-------------------- LAGM2021
Edited by capntrips7 (01/26/21 10:09 PM)
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GypsyBastard
Misanthrope


Registered: 11/30/20
Posts: 846
Loc: The Mighty Boosh
Last seen: 2 years, 10 months
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: capntrips7]
#27157034 - 01/19/21 08:53 AM (3 years, 9 days ago) |
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haha capntrips, as I read that I watched a short video in my head of your comedy of errors playing out. I enjoyed that very much. Thank you for sharing!
I'll upload the pic a bit later but I have a AA+ plate that was pissing me off. The print foil was not cooperating and it was towards the end of my session and back was killing me so I just said fuck it, held the print over the edge of the of the plate, scraped it with my scalpel and tapped the back to knock a bunch of spores onto the plate. As you can probably guess, this plate now stands as a shining example of why you should never do that.
My plan is to do some T2s today and maybe move the PFC over to T1(TBD). All of that is dependent on me finishing tiling a shower today so...... ya know.....
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roarkell
The Roar Master!



Registered: 08/29/19
Posts: 2,223
Loc: Southern WI
Last seen: 6 months, 21 days
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: GypsyBastard] 1
#27157491 - 01/19/21 01:16 PM (3 years, 9 days ago) |
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Gastronomicus
3-0-G



Registered: 03/31/05
Posts: 9,727
Last seen: 4 hours, 7 minutes
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: roarkell]
#27157494 - 01/19/21 01:18 PM (3 years, 9 days ago) |
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Nice plates
-------------------- Make my Funk the P Funk, I wants to get Funked up
LAGM2024
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mushhead
Potato Devourer



Registered: 08/22/14
Posts: 2,215
Loc: Dimension X-124
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Mr. Mushie]
#27157496 - 01/19/21 01:19 PM (3 years, 9 days ago) |
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Quote:
Mr. Mushie said:
Quote:
mushhead said: Jan the 12th.
 Update 1/18/21 Looks like the myc has finally taken ahold of the grain. Woot woot chicken foot.
Oh boy these grains look nice. No burst grains... link me to your methods.
Is this Rye?
I've been rocking it lately but always get a few burst when it's simmering. I'd rather that than a handful of under hydrated grains slip by and stall some jars.
This is actually winter wheat and I'd be delighted to write up my process for you. It'll take a minute for my grain to get to me as I order bulk from a local farm.
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D3_Myc
Weeb Trash



Registered: 05/06/18
Posts: 4,399
Loc: Year Zero
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: roarkell]
#27157619 - 01/19/21 02:35 PM (3 years, 9 days ago) |
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Hnng...oh spore daddy, you know what I like!

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roarkell
The Roar Master!



Registered: 08/29/19
Posts: 2,223
Loc: Southern WI
Last seen: 6 months, 21 days
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: D3_Myc]
#27157623 - 01/19/21 02:41 PM (3 years, 9 days ago) |
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Quote:
D3monic said: Hnng...oh spore daddy, you know what I like!


Right!? Did you see all the identical APEU except for one? Lol
Edit:4th pic top left... lol
Edited by roarkell (01/19/21 02:42 PM)
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Ballzagna
Carb Daddy



Registered: 10/09/19
Posts: 619
Last seen: 13 days, 14 hours
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: D3_Myc]
#27157632 - 01/19/21 02:49 PM (3 years, 9 days ago) |
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====1/19 UPDATE====
Yeti germ plate taking off on oats

Yeti and LPEU T1s ready for grain tomorrow after some transfers finally taken today. Tbh shoulda had them on grain like Sunday but life had other plans, also ran out of lids for new grain jars so making more set me back another day. Lost a couple LPEU plates, one to mold and one just didn't take, so 2 left and then 6 yeti

Pan Cyan plates are looking good I think. I read that you want fast wispy growth, I think the second one is more what I'm going for? Anyone with experience have some input? Gonna go get some millet and do up some jars for these.

APE is slow but that's to be expected. The original germ plate APE4 still looks decent, all the rest have been chucked. Only one T1 made it out without mold, slow going but looking ok to me.

I have some more catching up to do on everyone else's posts but looking good from what I've seen!
-------------------- No one likes a naysayer It's probably fine. Even if it's not fine, it'll eventually be fine. So it's fine. PE Adventure
Photosynthesis: A Morning Ritual Ballzagna's Tek Compendium and Notes for Noobs 
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Camera93
We got dicks like Jesus



Registered: 08/15/18
Posts: 3,220
Last seen: 10 days, 11 hours
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Ballzagna]
#27158282 - 01/19/21 08:23 PM (3 years, 9 days ago) |
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T1 going to T2 tonight
xfers from top left colony:
-------------------- All I need are some tasty waves, a cool buzz, and I’m fine. Whatever you decide won’t really impact our survival Close your eyes, and do the best that you can
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Narrator
Strangest in the night



Registered: 11/30/20
Posts: 187
Last seen: 1 year, 5 months
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: D3_Myc]
#27158354 - 01/19/21 09:06 PM (3 years, 9 days ago) |
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Update! Using new batch of LME plates with FURAN-2. 6g LME/8g telephone agar / 500ml distilled H20. Let the dishes sit for four days in SAB before getting around to using them, very little condensation.
Took APE T1s from the recently beautifully contaminated plate. A slice from where the battle was fought and two from a late germinating areas.

More APE T1s from other plates. How clean does the plate started 1.4 look?

T1s of the Puerto Ricans.

Giving up on the P. adiposa, perhaps will try again soon but have enough projects started for now.
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