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OfflinePitcherCrab
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Registered: 12/02/06
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: GrinchGrower]
    #27154551 - 01/18/21 01:32 AM (3 years, 10 days ago)

Quote:

GrinchGrower said:
I was still getting slight specks from malt in my agar until I broke out the immersion (stick) blender during the pre-boil.
Microwaving and shaking (w/ deionized water) wasn't dissolving everything well enough.




Any thoughts about throwing it in the blender after its boiling before putting it in the bottle for PCing? I don't have an immersion blender or a microwave so anything involving those is out unfortunately.


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PC's LAGM 2021 (TOC & TWC)
P. natalensis Growlog 2021
Pans for PC Fall 2021 Growlog

ā€œI must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.ā€ - Bene Gesserit Litany Against Fear



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OfflineGrinchGrower
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: PitcherCrab]
    #27154555 - 01/18/21 01:34 AM (3 years, 10 days ago)

Probably wouldn't hurt, but a whisk might be just as effective


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InvisibleD3_Myc
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Registered: 05/06/18
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: GrinchGrower]
    #27154558 - 01/18/21 01:35 AM (3 years, 10 days ago)

T2 transfers taken tonight.



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Invisiblebigfootscreepyuncl
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: GrinchGrower]
    #27154562 - 01/18/21 01:40 AM (3 years, 10 days ago)

I just hit it with a whisk for a minute and it worked well. My blender is not safe to use boiling liquids with (at least that's what the manufacturer says..I'm not gonna risk it though) so I'd just say to make sure you can put boiling water in yours.


My APE T-1 plates are crawling, so it's gonna be a while before I have a good update. My PE germ plate will be ready for T-1s in a couple of days. I definitely picked 2 of the slowest varieties for LAGM lol. Still no sign of GT...


--------------------



I :heart: 5318008


NOT a virgin!


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OfflineGrinchGrower
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: GrinchGrower]
    #27154612 - 01/18/21 02:59 AM (3 years, 10 days ago)

Quote:

GrinchGrower said:
- 01/18/20: T-2




Took two transfers from all five T-1 plates


Cracked the bottom of one parafilming  :shakefist:





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InvisibleD3_Myc
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: GrinchGrower]
    #27154619 - 01/18/21 03:10 AM (3 years, 10 days ago)

A lil tape and it might be fine


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OfflineGrinchGrower
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: D3_Myc]
    #27154634 - 01/18/21 03:16 AM (3 years, 10 days ago)

Not going to fuss around with a single donor plate too much...
Wish I had some grain ready I could have yeeted it in for the early fruit competition tho
:aweman:


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OfflineHaywire
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: PitcherCrab]
    #27154645 - 01/18/21 03:30 AM (3 years, 10 days ago)

Quote:

PitcherCrab said:
Any thoughts about throwing it in the blender after its boiling before putting it in the bottle for PCing? I don't have an immersion blender or a microwave so anything involving those is out unfortunately.





you can use blender or mixer but I don't bother doing both.
weigh dry ingredients, put in stir bar and place on stirrer. pour cold tap water and let stir for a minute or five.
then put in bottle and sterilize.
never had a bad batch doing it that way.


--------------------
Ciao mamma, guarda come mi diverto


My grows :mushroom2: Outdoor patches


Edited by Haywire (01/18/21 03:31 AM)


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InvisibleWall.E
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Wall.E]
    #27154698 - 01/18/21 04:32 AM (3 years, 10 days ago)

Quote:

Myc_Hunt said:
1/18/2021

Decided to try LC's for the first time with these 2 plates.

Ps. Natalensis



Jambos






--------------------
Life’s shit, but I’m loving it



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InvisibleJosex
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Wall.E]
    #27154701 - 01/18/21 04:40 AM (3 years, 10 days ago)

You used the poke? I never poke the edge, it might still work but I've heard people saying poking the edge never worked for them. The edge is usually only growing superficially on the agar, you want myc a tad further in from the edge where the culture is a little more rooted on the agar puck.

Me likes the look on those plates. :awesomenod:


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Invisibleallthespores


Registered: 12/14/20
Posts: 42
Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: allthespores]
    #27154718 - 01/18/21 05:04 AM (3 years, 10 days ago)

18.01.21

one week after inoculation and shaking one time.
a little bit of condensation, but i have observed it as normal in my incubator so im not worried.
noticed that the pan mycel really loved shaking, it looked a bit like stalling/slowing down before.
sadly i wont be able to finish this grow just now. work has come up faster then i had hoped, so the jars go in the fridge for about 5-6 weeks by the end of january.

picked up another 18 glas petri dishes. now i have enough for proper agar work. :mushroom2:


--------------------
LAGM2021 Grow log


Edited by allthespores (01/18/21 05:10 AM)


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InvisibleWall.E
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Josex]
    #27154774 - 01/18/21 06:20 AM (3 years, 10 days ago)

Quote:

Josex said:
You used the poke? I never poke the edge, it might still work but I've heard people saying poking the edge never worked for them. The edge is usually only growing superficially on the agar, you want myc a tad further in from the edge where the culture is a little more rooted on the agar puck.

Me likes the look on those plates. :awesomenod:




Maybe one day I'll actually follow a tek rofl. I'm gonna transfer from them then put to grain anyways as a back up. Figured I'd give lc a shot.


--------------------
Life’s shit, but I’m loving it



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OfflineGypsyBastard
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Wall.E]
    #27154982 - 01/18/21 09:06 AM (3 years, 10 days ago)

Whoa, lots of good discussion going on in here.... Where to start.....
I suppose it wouldn't hurt to add an agar recipe and procedure to my OP anyway.

Agar

While my plates are not like crystal, I really have no need to get them more clear than they are. While the mix and procedure is fairly customized to fit my needs and preferences I feel it's a pretty easy and straightforward way to get your plates within the top 5 percentile of clearest plates.

Telephone agar-7.5g
LME-15g
tap water-450ml

  • Pour 450ml of water into glass beaker.
  • Add stir bar, sit on mag stirrer. Turn on stir bar and heat.
  • Add LME to beaker -I don't dump it all a once but also not super slow. Maybe 10-15 seconds total time. Dumping it all at once would probably be fine, this is just a habit I developed when working with Potato Starch for PDA.
  • Add agar to beaker
  • Place thermometer in holder continue stirring/heating until a minimum of 185F is reached -I usually got to 200F but haven't gotten away with pulling at 185-186 without issue.
  • Pour into media bottles and PC


To serve a bit of a dual purpose, here's a pic of my GT T1 with some kickass growth on it. Of 20 plate sleeves, usually no more than 2 will have a bit of sediment like the one shown. I'm not too worried about10% having pieces like that, it's the cloudiness I hate.





If you check back to my update on the 17th you can see how much that GT has grown in less than 24 hours and I am stoked about that. I may go ahead and start taking T2s today. AA+ is looking very similar. Z still seems to be on the struggle bus but my have germination on the new PFC T0.

Well shit, I feel like there was something else I wanted to comment on in this post but after all the updating and uploading I can't remember.....
I'll copy this over to my OP as a mini update and see if it comes back to me.


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OfflineSvetaketu
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: GypsyBastard]
    #27155180 - 01/18/21 11:13 AM (3 years, 10 days ago)

Got around to taking T1's yesterday. all 6 germ plates looking good. took 8 transfers, 5 RW and 3 Lucy Cambo.

1/17/21
Lucy Cambos


Rusty Whites




My agar has a lot of sediments. guess it might be too much LME? not totally sure. last few batches have been 11.5g Agar & 8.5g LME.


--------------------
LAGM2020
LAGM2021


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OfflineThe Dalcassian
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Svetaketu] * 1
    #27155342 - 01/18/21 12:32 PM (3 years, 10 days ago)

Well that t1 I took to grain is jumping off. Bit of a shot in the dark but I've no idea what these spores will bring me so I wanna keep the gene pool as big as possible.



--------------------
Here Lies This Individual





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Invisiblemushhead
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Registered: 08/22/14
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: The Dalcassian]
    #27155392 - 01/18/21 12:51 PM (3 years, 10 days ago)

Quote:

The Dalcassian said:
Well that t1 I took to grain is jumping off. Bit of a shot in the dark but I've no idea what these spores will bring me so I wanna keep the gene pool as big as possible.





lemme get my T1 jar out and grab an update pic. I haven't been watching it at all.


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OfflineThe Dalcassian
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: mushhead]
    #27155443 - 01/18/21 01:10 PM (3 years, 10 days ago)

When did you drop yours in? Mine was Saturday.


--------------------
Here Lies This Individual





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Invisiblemushhead
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: The Dalcassian]
    #27155500 - 01/18/21 01:37 PM (3 years, 10 days ago)

Jan the 12th.

Update 1/18/21 Looks like the myc has finally taken ahold of the grain.
Woot woot chicken foot.


Edited by mushhead (01/18/21 01:47 PM)


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OfflineSockadin
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: mushhead]
    #27155528 - 01/18/21 01:52 PM (3 years, 10 days ago)

Well I guess it is time to start over. Long story but, I maybe made a TIT incubator! :shocked: and I know... But had to get some temps up and have been banished to the dungeon. So what do I do? I make a fucking lab out of it. Except it gets cold down here, average temps are 20-35 on a good day.

  Anyway I make a TIT like back in the day, but because of the holidays I couldn't find any good totes to use. So I might have dropped my plates into the bacteria laden water at the bottom of the big tote and the shitty cling wrap, (couldn't find Saran) didn't keep the water out.


  My little bit of germination has been wiped out by huge bacterial colonies and I think it is time to start over.

I will post some pictures soon.  Im still in this though!

:charliecoke:


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OfflineA.k.aM
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Sockadin]
    #27155549 - 01/18/21 01:59 PM (3 years, 10 days ago)

Lol that sucks man.


I had to do the same thing and accidentally stacked the plates too high so when I put the lid on a few cracked. So dumb.


I’m finally past the most boring part of lagm. Got a bunch of t1s and lcs to do.


--------------------
LAGM2020


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