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InvisibleBuddaking
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: MycoBrainz]
    #27143408 - 01/12/21 09:24 AM (3 years, 16 days ago)

I am hopefull I get home & see something similar one of us. I almost never get rizomorfic growth on germ plates.. I never win scratchers either so that is just my luck I guess.

Do yourun full nutrients on your germ plates ?


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OfflineTankie_J
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Registered: 01/09/19
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Doctor Mario]
    #27143409 - 01/12/21 09:25 AM (3 years, 16 days ago)

Quote:

Doctor Mario said:
Quote:

Tankie_J said:
Hey Gypsy I’m adding a new one to the mix:

OP update

Whytegold
-11JAN-
I received an awesome package from Filthyknees today and in it was a print labeled “Whytegold”. I haven’t read anything about this on the forum yet which has me pretty excited. It’s a rusty looking print (I didn’t get a pic of it). Used a sterile scalpel, scraped the print and jammed the spores into the agar. Not the best technique but I’m just not having any luck with the streak lately.






Have you only been using that pink color for your plates? Does it contain Red #3?

Quote:

Doctor Mario said:
I've seen no germination on the magenta plates and all transfers start to look something like this:


Its difficult to get a decent photo but the mycelium grew down inside of the agar and is expanding outward at a very slow rate. That plate was transferred on 9/2/20. The mycelium is sectoring strangely as well and I'm not sure why.







Fuck... it’s my first time running colored plates.(that actually stayed colored) Yes the neon purple has red#3. Used 1 drop per 100ml. Poured 20 plates with this. Will switch to blue or green next. Especially if issues arise.

The streaking issues have been on non-dyed LME plates. They were also streaked with a loop, flame sterilized and cooled on the agar before swiping the print. So, a few changes in agar prep and tools used. Will have to just run with it for now


Edited by Tankie_J (01/12/21 09:45 AM)


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InvisibleBuddaking
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Tankie_J]
    #27143426 - 01/12/21 09:38 AM (3 years, 16 days ago)

Fuk me too. I ran a very light purple to easily distinguish my un used germ mix rounds from others... :themoreyouknow:


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OfflineOne of Us
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Buddaking]
    #27143428 - 01/12/21 09:39 AM (3 years, 16 days ago)

Quote:

Buddaking said:
I am hopefull I get home & see something similar one of us. I almost never get rizomorfic growth on germ plates.. I never win scratchers either so that is just my luck I guess.

Do yourun full nutrients on your germ plates ?




Yep, I run ~2% nutes. I also use gentamicin for germ plates. Another thing worth mentioning about these particular plates is, although I originally made them with less agar powder for slightly "wetter" plates, I think I poured them in October. So they are a bit old and probably dried out a bit. Stored in a drawer.


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OfflineTankie_J
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Registered: 01/09/19
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Buddaking]
    #27143448 - 01/12/21 09:52 AM (3 years, 16 days ago)

The really fuckery dumb shit part of this....I read through that D3monic coloring thread a while back and completely forgot about it. I’m sitting here just shaking my head. I used store brand stuff so maybe there’s hope that they went cheap and it won’t effect growth as much as the gel stuff. Only time will tell


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OfflineBrownBear
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: BrownBear]
    #27143452 - 01/12/21 09:54 AM (3 years, 16 days ago)

Quote:

Iambrownbear said:
Quote:

Iambrownbear said:
This should be fun.

RustyWhyte 8 plates swabbed from a print.

1/1/21






Here is one of my RW plates. They germinated around 5 days after swiping with a swab. Gonna make some transfers this weekend.

1/8/21


My plan is to go to t3 before transfering to lc. From lc to 1/4 pint rice jars slurried to 3 qt popcorn bags. I am going to go with a 1:3 spawn to sub ratio with 1 qt of coir as a top layer.




I made my RW T1's on 1/9. Since these transfers recovered quickly and are looking nice, I will only take these to T2 before going to lc.



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Invisiblebigfootscreepyuncl
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: BrownBear]
    #27143495 - 01/12/21 10:16 AM (3 years, 16 days ago)

That's some really nice growth brownbear! My APE germination plate is still about the size of a dime but is showing a couple nice areas to take transfers from. I'm not sure if the slow growth is due to the purple food coloring I used, whether it's too nutrient dense or if APE is just this slow.

Anyway, I'm waiting on some new media bottles to show up then I can pour some more plates and get moving. I'm still waiting on PE and GT to germinate.


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Invisiblemushhead
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: filthyknees]
    #27143573 - 01/12/21 10:46 AM (3 years, 16 days ago)

Quote:

filthyknees said:
Quote:

mushhead said:

First Burma agar2grain
Next up g2g.
:teabird:
Not much yet to show for the Pan cints.
It's all just agar work rn, I'll get pics later once I have some nicer plates.



Is that your germ plate or did you take t1 like six hours in? Lol. Looks like a transfer?

My t1s are like the size of a dime right now transfered four days ago.



That's a T2 plate from the germ plate.
I didn't let it finish colonizing the plate like I usually do.
It looks way ugly, there's still a lot of tomentose growth in there.
I use 1g LME to 500ml and 10g agar which made this Burma culture get speedy as fuck.

This is the germ plate from 01/01/2021 8am.
I also want to point out that I have all day every day to mush cult. Working from home allows me to do my day job and mycology at the exact same time.


Edited by mushhead (01/12/21 11:05 AM)


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OfflineMadscience039
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Buddaking]
    #27143605 - 01/12/21 11:00 AM (3 years, 16 days ago)

I’ve got germ on my albino mak/ PG plates. 4/5. One went bacterial and didn’t need it. Tomentose so far, to be expected at initial transfer. T1 got done last night.


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InvisibleJosex
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Madscience039] * 1
    #27143626 - 01/12/21 11:10 AM (3 years, 16 days ago)

Finally got germination on the plates inoculated on the 1st. :cool:


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OfflineMadscience039
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Josex]
    #27143632 - 01/12/21 11:14 AM (3 years, 16 days ago)

Good shit josex. Running those giant PE huh?


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Offlinetiptrippy
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Josex] * 1
    #27143636 - 01/12/21 11:15 AM (3 years, 16 days ago)

You guys I already have pan cyan t1 or t2 from germ on 1/1 on grain. I got you all beat lol


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InvisibleJosex
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Madscience039]
    #27143645 - 01/12/21 11:19 AM (3 years, 16 days ago)

Quote:

Madscience039 said:
Good shit josex. Running those giant PE huh?




I hope lol don't know what I'll get.


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Invisiblemushhead
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: tiptrippy]
    #27143650 - 01/12/21 11:23 AM (3 years, 16 days ago)

Those pans are yes very speedy.
I have up to T5 plates rn of it and they're all just not looking great.
They're uneven in growth, I'm not looking for rhizos because Pans are different.
I'm also working with pan cinctulus, so mayhaps I'm looking for the wrong kind of myc.
I've never worked with pans before. Pant cints are my first try.


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OfflinePitcherCrab
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: mushhead]
    #27143754 - 01/12/21 12:20 PM (3 years, 16 days ago)

1/12/2021
Just made 3 transfers each of TWC and TOC. Moving right along! :royalrainbow:



Updated original post here: https://www.shroomery.org/forums/showflat.php/Number/27125101#27125101


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PC's LAGM 2021 (TOC & TWC)
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“I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear



Edited by PitcherCrab (01/12/21 12:41 PM)


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OfflinePuduwoke
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: PitcherCrab] * 1
    #27143794 - 01/12/21 12:37 PM (3 years, 16 days ago)

Make T1 of APE and Melmac a few minutes ago. First time getting ape to germ :rockon:


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InvisibleJosex
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Puduwoke]
    #27143796 - 01/12/21 12:38 PM (3 years, 16 days ago)

:mostinteresting:


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Invisiblemushhead
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Puduwoke] * 1
    #27143798 - 01/12/21 12:39 PM (3 years, 16 days ago)

Quote:

Puduwoke said:
Make T1 of APE and Melmac a few minutes ago. First time getting ape to germ :rockon:



So proud Puduwoke!!

This is my first time getting a clean useable culture of APE so I understand how you're feeling. Congrats!!


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InvisibleJosex
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: mushhead]
    #27143801 - 01/12/21 12:42 PM (3 years, 16 days ago)

That's a gorgeous plate mushhead. APE loves its rhizos for sure.


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OfflineSold Out Online
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Josex] * 1
    #27143805 - 01/12/21 12:44 PM (3 years, 16 days ago)

Just got this awesome package in the mail from Filthyknees.



Now, like a kid in a candy store, I’m not quite sure what to do with myself. I’m looking at how many tubs I have, and how much space I have, and how many strains I currently have on the go... probably gonna to have to do some top fruiting of grain jars or something so I can get a look at, and some prints of, some of these things.

Took some of the APEU to plate. Had to start somewhere...



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Threads that have been useful to me:
Clean Spawn checklist for new growers


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