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OfflineFourneau420
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Registered: 08/22/20
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Last seen: 10 days, 14 hours
Colony stopped growing
    #27102237 - 12/22/20 11:28 AM (1 month, 2 days ago)

Hi guys , its been between 24 and 48 hours since its stopped colonizing any tips ?


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OfflineB21
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Registered: 04/09/20
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Re: Colony stopped growing [Re: Fourneau420]
    #27102338 - 12/22/20 12:57 PM (1 month, 2 days ago)

I hate to say it but that looks bacterial. I think you should try again. Idk if you have or not but look into agar work. Imo all noobs should start with agar to grain or pf tek (if you really don’t want to learn agar). Also start off with grain in quart size jars. I couldn’t imagine using bags when I was new. Good luck 👍


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InvisiblezManS
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Re: Colony stopped growing [Re: B21]
    #27103728 - 12/23/20 10:30 AM (1 month, 1 day ago)

I would shake it one more time. If it recovers you're all good, if it doesn't it was contaminated.


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OfflineB21
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Re: Colony stopped growing [Re: zMan]
    #27104034 - 12/23/20 01:42 PM (1 month, 1 day ago)

It’s contaminated


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OfflineTigerStrike
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Registered: 12/22/20
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Re: Colony stopped growing [Re: B21]
    #27104044 - 12/23/20 01:45 PM (1 month, 1 day ago)

That is a lot of good colonized area and grains are not free. No birth and scrape?


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OfflineB21
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Re: Colony stopped growing [Re: TigerStrike]
    #27104054 - 12/23/20 01:48 PM (1 month, 1 day ago)

I guess bad spawn denial really is a thing


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OfflineFourneau420
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Re: Colony stopped growing [Re: B21]
    #27104068 - 12/23/20 01:59 PM (1 month, 1 day ago)

How could it be contaminated since the growing was a1 , no trace of contamination , the only thing it seem dry(mycelium 2 )


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OfflineB21
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Re: Colony stopped growing [Re: Fourneau420]
    #27104142 - 12/23/20 02:30 PM (1 month, 1 day ago)

I really do hope I’m wrong but ime when myc looks kinda like melted ice cream and it’s all sketch looking like that it’s no use in trying. But do what you think is right... I’m just trying to help. I want to see you grow wall to wall canopies! I just don’t think it’s gonna come from that bag of grains. While you’re holding on to hope with this bag start a new project! Good luck.


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OfflineFourneau420
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Re: Colony stopped growing [Re: B21]
    #27104239 - 12/23/20 03:28 PM (1 month, 1 day ago)

Yeah , i know ,im not saying your wrong and i am right, since i never harvested . So im.still going by trials and error lol


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OfflineTigerStrike
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Re: Colony stopped growing [Re: Fourneau420]
    #27104479 - 12/23/20 06:31 PM (1 month, 1 day ago)

You know I though the same thing that it looked clean then, I assumed somehow, he inoculated the bottom and it worked itself up toward the bag opening then stalled in the top Now I'm not sure.


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OfflineFourneau420
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Re: Colony stopped growing [Re: TigerStrike]
    #27104837 - 12/23/20 10:18 PM (1 month, 1 day ago)

Maybe the mycelium dry up because ive but it in a carton box that i made huge vent hole but maybe it was too dry and the temp of twice a day 23/24 celcius .. like i said its still my trials and error phase i'll get there


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InvisiblezManS
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Re: Colony stopped growing [Re: Fourneau420]
    #27109374 - 12/26/20 07:02 PM (29 days, 6 hours ago)

If you soaked the grain it shouldn't have dried out. Like mentioned above its very very likely contaminated but if you want to be sure. Shake it,  if it recovers you might have something but there's a huge chance it will never recover as it's contaminated.


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OfflineJoBloShow
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Re: Colony stopped growing [Re: zMan]
    #27109666 - 12/26/20 09:58 PM (29 days, 3 hours ago)

B21 is right, that spawn is bacterial.  That's the bad news.  The good news is that all may not be lost.
If I were you, I'd mix up that spawn with some CVG and make a couple shoeboxes.  You might get lucky and pull out a flush before it contaminates.
But as I said, all may not be lost. There's no guarantee you'll get anything before contamination sets in.

Definitely start a new project and don't hold too much hope on this bag.

Best of luck!


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We'll ride the spiral to the end and may just go where no one's been...



FIND THE BEAUTY IN THE DISSONANCE


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OfflineFourneau420
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Re: Colony stopped growing [Re: JoBloShow]
    #27113778 - 12/29/20 11:00 AM (26 days, 14 hours ago)

Ok , it weird if it would be contaminant it would grow or multiply , but 84 hours in still the same as the pict, no color except white


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InvisiblezManS
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Re: Colony stopped growing [Re: Fourneau420]
    #27113794 - 12/29/20 11:14 AM (26 days, 14 hours ago)

Quote:

Fourneau420 said:
Ok , it weird if it would be contaminant it would grow or multiply , but 84 hours in still the same as the pict, no color except white




Did it grow at all or same size? If it hasn't grown its contaminated.


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OfflineFourneau420
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Re: Colony stopped growing [Re: zMan]
    #27114042 - 12/29/20 01:34 PM (26 days, 12 hours ago)

Ok , last questions , i suspect my spore print is contaminated bought it from spores101 becauses they sold in canada , i tried every sponsor here


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InvisiblezManS
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Re: Colony stopped growing [Re: Fourneau420]
    #27114930 - 12/29/20 10:44 PM (26 days, 2 hours ago)

Its possible,  get some agar and clean it up. Even with syringes its best to use agar and get a clean culture.


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Offlinepatapon333
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Re: Colony stopped growing [Re: zMan]
    #27115550 - 12/30/20 09:00 AM (25 days, 16 hours ago)

it may be contam there if it stops - shake it and wait, if it does not stink and it regenerate its everything ok :smile:


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OfflineFourneau420
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Re: Colony stopped growing [Re: patapon333]
    #27115768 - 12/30/20 11:06 AM (25 days, 14 hours ago)

I dont have , a pc nor a autoclave so i need toorder online and i dont think agar sell in slantbor in petri dishes and im not familiar with the procedure so if you guys can give hint and tips or sent me a link to buy agar online in canada  i will buy 4 sure


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OfflineB21
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Re: Colony stopped growing [Re: patapon333]
    #27117659 - 12/31/20 09:15 AM (24 days, 16 hours ago)

Seems like you’re getting ahead of yourself. If you don’t have a pc then I’d say pf cakes are your best option. You should really invest in a pressure cooker if you want to do bulk grows. That’s it...pc or your screwed. Pre made bags can work but if your putting ms straight to grain, IMO, your looking at contamination 9 times out of 10. You’ll even save more money in the long run if you just buy the pc now.
Buy pressure cooker
Learn agar
Read more. Everything I know now I learned by myself reading through tons of threads here at the shroomery. You got this! GL


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