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OfflineFietchen
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Registered: 08/27/19
Posts: 233
Last seen: 2 years, 8 months
Syringe Filter will not hold between the silicon lid
    #27100319 - 12/21/20 05:17 AM (3 years, 1 month ago)

Hello,

this is the second day and i dont know why the Syringe Filters not holding between the silicon in the hole :frown: i wait 24 hours and after pressure cooking the Filter are litte loose when i try to turn them.

What i doing wrong and what i need to do better?

I PC for 25 minutes



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Offlinestarbones
I'm an alien, I eat uranium.
I'm a teapot


Registered: 03/04/20
Posts: 1,131
Last seen: 2 months, 17 days
Re: Syringe Filter will not hold between the silicon lid [Re: Fietchen] * 1
    #27100371 - 12/21/20 06:24 AM (3 years, 1 month ago)

Silicone it to the lid.

I put a ring of silicone around the tit that's going to go through my lid hole. I push it on and the silicone expands outwards to the edge of the syringe filter completely coating the bottom of it where it then mates to the lid. I give it a twist to ensure the silicone is 100% covering the area between the lid and the bottom of the filter.

For added protection you can make the hole a little bigger so the silicone squeezes through to the other side of the lid then apply silicone there before the top side cures so the silicone on the bottom mates with the silicone on the top forming a plug that will keep the unit in.


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Listen, I'm steel fisted with the iron lung
Heavy metal ballads out the guitar where lions run.




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InvisibleZifozonke
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Registered: 03/24/19
Posts: 1,258
Re: Syringe Filter will not hold between the silicon lid [Re: starbones] * 1
    #27100748 - 12/21/20 11:45 AM (3 years, 1 month ago)

Lightly scuffing the lid round the holes and the underside of your filter with some sand paper will help give the silicone something extra to "grab" onto.


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OfflineFietchen
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Registered: 08/27/19
Posts: 233
Last seen: 2 years, 8 months
Re: Syringe Filter will not hold between the silicon lid [Re: Zifozonke]
    #27100811 - 12/21/20 12:21 PM (3 years, 1 month ago)

The Silicon is the right one high Temps silicone ID the right choice. I think it's good know i try not to touch the filter.

How much time it's needed to show sign of mycelium in the Liquid Culture? I inject 2 cc spores from the Syringe. I can't see any spores in the Syringe hope the seller don't sell me some shit. Than I hope I don't make a big mistake sucking litte air in my spore syringe after I pull the needle out of the Silicone lid ;(


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InvisibleZifozonke
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Registered: 03/24/19
Posts: 1,258
Re: Syringe Filter will not hold between the silicon lid [Re: Fietchen]
    #27100854 - 12/21/20 12:43 PM (3 years, 1 month ago)

Spores to lc/grain can be risky unless your syringe is super clean
Always best start on agar
If you not up to trying agar BRF cakes could be another option
*Mycelium can take a couple days to a week to show


Edited by Zifozonke (12/21/20 12:44 PM)


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OfflineFietchen
Stranger
Registered: 08/27/19
Posts: 233
Last seen: 2 years, 8 months
Re: Syringe Filter will not hold between the silicon lid [Re: Zifozonke]
    #27102121 - 12/22/20 07:40 AM (3 years, 1 month ago)

Where i can find the right syringe for suck out the lc when it's ready? Which needle? Thx


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