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OfflineNobodycare
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Tissue to agar growth
    #27099733 - 12/20/20 05:48 PM (3 years, 1 month ago)

Did my first 5 clones to plates 3 days ago. None of thethem have any growth of any kind. Is this normal? Do I just need to be patient? Ive read growth should occur within 1-3 days.



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Offlinekanemush
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Re: Tissue to agar growth [Re: Nobodycare]
    #27099759 - 12/20/20 06:14 PM (3 years, 1 month ago)

give it 7-14 days at least


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OfflineTheBoJim
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Re: Tissue to agar growth [Re: kanemush]
    #27099762 - 12/20/20 06:16 PM (3 years, 1 month ago)

i usually see the tissue get fuzzy in a day or two. what is your method?


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OfflineLeon ForestFacebookDiscord
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Re: Tissue to agar growth [Re: TheBoJim]
    #27099775 - 12/20/20 06:23 PM (3 years, 1 month ago)

Where did you take the tissue sample from? Is it a pin or did you use the pinch and tear method to get a piece from the inside of the fruit? Is it a wild tissue sample, was it fresh or has it sat long since the fruit was harvested?


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OfflinecronicrFacebook
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Re: Tissue to agar growth [Re: Leon Forest] * 1
    #27099852 - 12/20/20 07:19 PM (3 years, 1 month ago)

Somebody should right a tek on taking agar pics


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Offlinejcm4620
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Re: Tissue to agar growth [Re: cronicr]
    #27099854 - 12/20/20 07:21 PM (3 years, 1 month ago)

Quote:

cronicr said:
Somebody should right a tek on taking agar pics



πŸ˜‚πŸ˜‚πŸ˜‚πŸ˜‚ amen brother amen

i know mine aint perfect either but i always try to atleast make it visable.


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PANAEOLUS FRUITING MADE SIMPLE



Edited by jcm4620 (12/20/20 07:23 PM)


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OfflineLeon ForestFacebookDiscord
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Re: Tissue to agar growth [Re: cronicr] * 1
    #27099859 - 12/20/20 07:25 PM (3 years, 1 month ago)

Quote:

cronicr said:
Somebody should right a tek on taking agar pics



Step one, focus on the weed in the background.


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OfflinecronicrFacebook
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Re: Tissue to agar growth [Re: Leon Forest]
    #27099864 - 12/20/20 07:30 PM (3 years, 1 month ago)

:lol:


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Offlinejcm4620
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Re: Tissue to agar growth [Re: Leon Forest]
    #27099866 - 12/20/20 07:31 PM (3 years, 1 month ago)

step 2 make sure your phone is visable in the reflectionπŸ˜‰


--------------------
PANAEOLUS FRUITING MADE SIMPLE



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Offlinekanemush
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Re: Tissue to agar growth [Re: Leon Forest]
    #27099908 - 12/20/20 08:00 PM (3 years, 1 month ago)

can we get a better shot of the weed please :smile:
Quote:

Indulgence said:
Quote:

cronicr said:
Somebody should right a tek on taking agar pics



Step one, focus on the weed in the background.




can we get a better shot of the weed please :smile:


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OfflineNobodycare
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Re: Tissue to agar growth [Re: jcm4620]
    #27099909 - 12/20/20 08:00 PM (3 years, 1 month ago)

Sheesh..sorry for the rough pic. It was 5 sep fully grown fruits. Torn open and samples from middle of stem. Im thinking maybe the scalpel was still too hot maybe?


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OfflineNobodycare
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Re: Tissue to agar growth [Re: Nobodycare]
    #27099915 - 12/20/20 08:03 PM (3 years, 1 month ago)



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OfflineNichrome
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Re: Tissue to agar growth [Re: Nobodycare]
    #27099970 - 12/20/20 08:54 PM (3 years, 1 month ago)

Looks like it is growing.


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β€œBetter to be deprived of food for three days, than tea for one.”


Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson


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InvisibleLemgrub
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Re: Tissue to agar growth [Re: Nichrome]
    #27100105 - 12/20/20 11:04 PM (3 years, 1 month ago)

It can take a little bit for tissue to switch growth strategies sometimes. I've recently waited 7-10 days for an inner tissue biopsy to start taking to agar. As long as you get a clean sample, it should start growing.


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We getting that pirate bay, alien shishkebab cordycep money


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