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Offlinebiedrek
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First MS to agar attempt
    #27099816 - 12/20/20 06:55 PM (3 years, 1 month ago)

Hello,

I was wondering is this healthy mycelium or is it mold? First time so trying to get the eye for it on agar!



These are pictures of three different dishes

Any input will be appreciated


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InvisibleIdiotCircusBoy
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Re: First MS to agar attempt [Re: biedrek]
    #27099820 - 12/20/20 06:57 PM (3 years, 1 month ago)

Not looking good so far, sorry.


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Offlinebiedrek
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Re: First MS to agar attempt [Re: IdiotCircusBoy]
    #27099845 - 12/20/20 07:13 PM (3 years, 1 month ago)

I believe I poured my agar to hot caused condensation in the Petri dishes smh oh well live and learn
Streaked it also so I’d hope I could of saved them
Any more input would be nice


Edited by biedrek (12/20/20 07:14 PM)


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OfflineFrostyStipe
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Re: First MS to agar attempt [Re: biedrek]
    #27099874 - 12/20/20 07:41 PM (3 years, 1 month ago)

I've had the same problem going from mss to agar. I've been really careful to only place a single drop but no luck.

I've had no problems going from a swab or print, so there's that options.

Next time I try from mss, I'm going to pc a shotglass. Place a drop of solution into the sterile shotglass. Flame a loop,(or a needle, scalpel, whatever you use), cool in the agar, dip that into the solution and try to swab that way.

I've seen this suggested a few times but I'm not sure if there's a tek to credit.


Edited by FrostyStipe (12/20/20 07:42 PM)


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Offlinejcm4620
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Re: First MS to agar attempt [Re: biedrek] * 1
    #27099879 - 12/20/20 07:44 PM (3 years, 1 month ago)

pour more plates and review your sterile technique to find out where u went wrong. tthose r all trash. and bacterial.


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Offlinebiedrek
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Re: First MS to agar attempt [Re: FrostyStipe]
    #27099880 - 12/20/20 07:44 PM (3 years, 1 month ago)

Okayyy I guess I’ll scrap those and restart

I just inoculated one more agar without the streaking method we’ll see how that goes


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Offlinejcm4620
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Re: First MS to agar attempt [Re: jcm4620]
    #27099881 - 12/20/20 07:45 PM (3 years, 1 month ago)

also use prints cuz syringes suck


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Offlinebiedrek
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Re: First MS to agar attempt [Re: jcm4620]
    #27099886 - 12/20/20 07:49 PM (3 years, 1 month ago)

Well I never have issues with inoculating jars ever
I believe I just need to restart my agar


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OfflineShroomoisseur
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Re: First MS to agar attempt [Re: biedrek]
    #27099966 - 12/20/20 08:47 PM (3 years, 1 month ago)

It’s your syringes, I’m using no pour plates, all ms and have had great growth so far no contams at all; got my first contam on a swab plate recently. From one of the longest sponsors


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