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InvisibleZifozonke
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Registered: 03/24/19
Posts: 1,258
Preserving a clone growing in Muda style container
    #27098565 - 12/19/20 11:38 PM (3 years, 1 month ago)

So heres my dilemma...
I took a clone from a nice cluster of PE a while back.
Ended up making a liquid culture from a agar wedge taken off the plate of this clone&had a go at trying to test it and the tub ended up tamming on me.
Started a few mudajar containers and used up the last bit of this lc in one

Threw a very thin layer of casing and it didn't blob at all and chucked out a full flush of miniature Peens

So now ..I really want to have another go with this culture but I ended by mistake throwing away the plate that the wedge came from 🤦🏼‍♂️ and no more LC...
What's the best way to go about trying to preserve this one-I tried searching older threads but couldn't find much&it's already a clone ..so do I

1)take another clone (have read this is not a good practice) or
2)should I break apart the cake and put a few grains on agar or
3)just try getting a swab off one of the bigger fruits and start again

Would love to be able to produce the results of this test again scaled up
Any advice that would lead me in the right direction would be much appreciated


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OfflineLeon ForestFacebookDiscord
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Registered: 02/11/13
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Re: Preserving a clone growing in Muda style container [Re: Zifozonke]
    #27098652 - 12/20/20 01:29 AM (3 years, 1 month ago)

Reclone and restart from swabs/spores


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OfflineSockadin
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Registered: 01/03/10
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Re: Preserving a clone growing in Muda style container [Re: Leon Forest]
    #27098757 - 12/20/20 04:45 AM (3 years, 1 month ago)

If you have fresh fruits just come from the inside of one of the fruits to agar. Best way to further the genetics.


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InvisibleZifozonke
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Registered: 03/24/19
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Re: Preserving a clone growing in Muda style container [Re: Sockadin]
    #27099175 - 12/20/20 11:20 AM (3 years, 1 month ago)

Thanks for that Indulgence&Sockadin:rockon:
Kinda cover all bases and hopefully shoot for  similar results again:mushroom2::mushroom2::mushroom2::mushroom2::mushroom2:


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Invisiblech0ppie
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Registered: 11/18/20
Posts: 593
Re: Preserving a clone growing in Muda style container [Re: Zifozonke]
    #27099181 - 12/20/20 11:25 AM (3 years, 1 month ago)

Hi Zif
cron edit: don't be a teapot


Edited by cronicr (12/20/20 08:26 PM)


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InvisibleMadMuncher
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Registered: 10/27/12
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Re: Preserving a clone growing in Muda style container [Re: ch0ppie]
    #27099931 - 12/20/20 08:16 PM (3 years, 1 month ago)

wtf


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