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OfflineOVTHEEMOON
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Registered: 11/02/20
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1st Agar Attempt Syringe-Agar
    #27097656 - 12/19/20 12:15 PM (3 years, 1 month ago)

So Im in the middle of my first grow and decided to give agar a shot with the last bit of spore syringe I had left over, I think I may have used too much?? Not sure what to do with this since I cant seem to find any http://pics out there of people with a similar specimen, Help?


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Invisiblebigfootscreepyuncl
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Re: 1st Agar Attempt Syringe-Agar [Re: OVTHEEMOON]
    #27097736 - 12/19/20 01:07 PM (3 years, 1 month ago)

It's hard to tell from that picture if there's anything worth transferring or not, but it definitely looks contaminated. I would personally trash it and move on. Did you use an MS syringe? If so you literally only need a single drop, then swipe it with a flame sterilized inoculation loop. Cheers, mate!


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Offlineanatomality
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Re: 1st Agar Attempt Syringe-Agar [Re: bigfootscreepyuncl]
    #27097743 - 12/19/20 01:12 PM (3 years, 1 month ago)

Good on you for trying, looks like a bunch of mycelium. It would be hard to tell if you have anything else than mycelium mixed in as it is so fluffy and chaotic. Some of that doesn't look good, and maybe the agar itself was contaminated to begin with. Did the ones you didn't inoculate also end up looking like this?

Like the guy above me said, a single drop is enough and you let it grow out in a circle around it.
This is probably contaminated, but maybe not.

You could always try a 'poke' from the Josex (I think) thread, and transfer that to a new agar and see what you get. Once you get something that looks clean then you can put it to grain.


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OfflineDr.Sparkle
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Re: 1st Agar Attempt Syringe-Agar [Re: bigfootscreepyuncl]
    #27097749 - 12/19/20 01:13 PM (3 years, 1 month ago)

I have in the past had super loaded plates from syringes and managed to get myc from it, its not best practice thats for sure. Its much better to be able to see the individual colonies and transfer from them, so you can avoid contamination.


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OfflineOVTHEEMOON
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Re: 1st Agar Attempt Syringe-Agar [Re: OVTHEEMOON]
    #27097871 - 12/19/20 02:34 PM (3 years, 1 month ago)

I had one other jar that definitely was contaminated, all the growth in the this jar is fluffy and pure white. Sorry for the shitty pic, I didnt want to risk it by opening the jar just for a pic. I see now that I didn't dissolve my agar all the way which is what I think is the discoloration in the pic. I think Im gonna take look a try a couple "clean" looking samples and transfer to a completely dissolved clear agar plate and see what happens. And this was a multi spore of PE6 or Purp. Mystic.. forgot to label the lid.. sigh.


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InvisibleSir Pentinite
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Re: 1st Agar Attempt Syringe-Agar [Re: OVTHEEMOON]
    #27098119 - 12/19/20 05:18 PM (3 years, 1 month ago)

Definitely do not open it just for a photo. It looks like gray mold nearest the camera, but there's probably something worth transferring from the dense white growth behind it. Just try to get something good that is as far from obvious mold as possible.


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"I thought to myself 'Boy, I'm sure glad there's nobody here to see this because this is exactly the sort of thing that gets people riled-up and they assume you're dying and that something has to be done. Where if you're alone, you know, you either come through it or you die, but in any case you avoid the fuss.'"
- Terrence McKenna


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OfflineGroo
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Re: 1st Agar Attempt Syringe-Agar [Re: Sir Pentinite]
    #27098203 - 12/19/20 06:12 PM (3 years, 1 month ago)

These guys all smoke crack. Put it in a SAB and carefully transfer 10-15 plates and pray then xfer agan untill you can get something clean. If you dont treat this as if its the last sport syringe in the world and sage this 1 strain itll be the end of the line.


Edited by Groo (12/19/20 06:13 PM)


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OfflineDr.Sparkle
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Re: 1st Agar Attempt Syringe-Agar [Re: Groo]
    #27098357 - 12/19/20 07:31 PM (3 years, 1 month ago)

Mmmm crack


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InvisibleIdiotCircusBoy
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Re: 1st Agar Attempt Syringe-Agar [Re: Dr.Sparkle]
    #27098366 - 12/19/20 07:42 PM (3 years, 1 month ago)

:whathesaid:


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