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OfflineB21
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Clone questions
    #27097146 - 12/19/20 06:51 AM (3 years, 1 month ago)

If I put ms to agar, make two xfrs, and on the second xfr plate a pin pops up...I clone that pin. Grow it out, make a xfr and inoculate with that plate. (Ms>agar>xfr>xfr>clone pin>xfr>inoculate>spawn to bulk). Is it a good idea or bad idea to clone a fruit that comes from this tub?
Like wouldn’t that agar pin clone still contain tons of different genetics? It’s basically still multispore since not many xfrs have been made right? Sorry I’m just trying to understand. I’m still searching around through posts but was hoping a pro could clarify this for me.


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InvisibleThe Fresh PrintsS
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Re: Clone questions [Re: B21]
    #27097174 - 12/19/20 07:15 AM (3 years, 1 month ago)

Curious about this as well. I've been operating under the assumption that there would be more genetic variance in a plate pin than a proper tissue sample clone.


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Offlinejcm4620
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Re: Clone questions [Re: The Fresh Prints]
    #27097203 - 12/19/20 07:38 AM (3 years, 1 month ago)

i never clone from a clone. kinda like havin a baby with ur cousin or ur sister

i mean u may get away with it but its just not a good practice in my book


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Edited by jcm4620 (12/19/20 07:40 AM)


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Offlinenogoodnamesleft
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Re: Clone questions [Re: jcm4620]
    #27097236 - 12/19/20 08:03 AM (3 years, 1 month ago)

plate pins are regarded to generally be a good fruiting sub culture of the variety one germinated spores of as an agar plate is a less than ideal substrate for fruiting.


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InvisibleThe Fresh PrintsS
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Re: Clone questions [Re: nogoodnamesleft]
    #27097251 - 12/19/20 08:10 AM (3 years, 1 month ago)

I don't think that was OPs question.


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Offlinebw86
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Re: Clone questions [Re: The Fresh Prints]
    #27097437 - 12/19/20 10:26 AM (3 years, 1 month ago)

I think what the question boils down to is; Isi harm in isolating genes any further than a MS pin clone. Since theoretically there are more(?) genes in that pin then there are in a mature fruit.

Don't quote me on tis but i think i remember reading there is a 20% reduction in genes every-time you clone a fruit.
-In all reality there is no saying if any isolation is worth doing until its done and tested.


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