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manlord
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1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? *DELETED*
#27096582 - 12/18/20 08:06 PM (3 years, 1 month ago) |
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Post deleted by manlord
Reason for deletion: makes me look like huge noob
Edited by manlord (12/18/20 10:40 PM)
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bw86
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Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: manlord]
#27096622 - 12/18/20 08:31 PM (3 years, 1 month ago) |
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Unfortunately there is no cubensis mycelium in any of those plates you have to look up better pictures.
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ChRnZN
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Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: bw86]
#27096624 - 12/18/20 08:33 PM (3 years, 1 month ago) |
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Mycelium should be thready
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manlord
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Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: ChRnZN]
#27096638 - 12/18/20 08:42 PM (3 years, 1 month ago) |
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What about the PE transfers? I mean you can def see thready growth on the wedges I transferred in the center, but its not leading to better growth. Do you think I got messed up syringes, or what would lead to that watery looking myc? Is every plate a contam, possibly every syringe I got was contam? I mean Idk if everything on my plates is contam, I can clearly see the growth after I add spores, and none of my other plates I have just sitting out are contaminating. The PE showed some ropeyness, I know its hard to tell from the pics, but I transferred the more ropey parts but it hasn't led to more rhizo growth so far. The B+ I'm lost on, how could multiple syringes produce the exact same looking contam, if thats what it is, on like 10 different plates? Is my agar recipe too watery, idk what could be causing this. Any ideas on casusation?
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manlord
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Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: ChRnZN]
#27096652 - 12/18/20 08:50 PM (3 years, 1 month ago) |
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Yeah I feel you! I realize this is unhealthy looking myc, hence the post. I'm looking for ANY possible reason this could be happening to like 20 plates, no signs of contam in blank plates left out at same location. Could both of the 2 B+ syringes I used be carrying a similar contam thats infecting each plate? Idk sounds unlikely but could be the case. I'm more thinking that this is just reallyy unhealthy myc or something. Should I try a spore print and assume these MS syringes are trash? I'm just wondering what went wrong here
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ChRnZN
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Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: manlord]
#27096669 - 12/18/20 09:00 PM (3 years, 1 month ago) |
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The problem could be bacterial contamination in the water from the syringes. I like spore prints myself. Freezing them before usage decreases contamination. Make sure your environment is clean. Lots of mycelium killers are floating around us all the time. Concerning your jars' placement - a shelf by the sun at 65 F would be best.
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manlord
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Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: ChRnZN]
#27096691 - 12/18/20 09:12 PM (3 years, 1 month ago) |
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Just bought a spore print from a sponsor, yeah I'm hoping it's just the syringes. I have a room I use as the lab and i spray air sanitizer alot and run the flowhood regularly in there so I doubt I have a bad airborne contam proble. Like I said none of the plates I have sitting out have shown any growth at. And 65degrees? I've heard like 75-80 for incubating myc am I running too hot? I've got them at around 79 with this lava rocks electric thing and I could save money and turn that off and theyd never be below 68
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PBJ710
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Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: manlord]
#27096819 - 12/18/20 10:41 PM (3 years, 1 month ago) |
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Are you boiling the water before adding your LME/Agar powder? The agar itself looks really soft in the first couple pics. I would ditch the yeast at least temporarily to see how it effects the agar. Not sure what a 'lava rocks electric thing' is, but you don't need any heaters as long as you are comfortable in the room. If you have to use a heater, make sure that it's indirect heating or it can fry your mycelium.
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manlord
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Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: PBJ710]
#27096840 - 12/18/20 10:52 PM (3 years, 1 month ago) |
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No, I haven't boiled the water before adding the agar/malt ex. Does that help? I'll try that and ditch the yeast maybe it's the agar, that would make sense why every plate is off and why the spores seem so watery/runny. I'm thinking it's got to be either the agar itself or the spore syringes, or both. And the lava rocks thing is just a really small electric heater that lightly heats up a little pan of lava rocks which generates a small amount of heat. The jars are a far enough away where its not like direct heat, just ups the temp a few degrees in the room. But if not necessary, I'll save the money and shut it off to save $.
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PBJ710
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Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: manlord]
#27097125 - 12/19/20 06:31 AM (3 years, 1 month ago) |
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Not 100% sure that it's 'the answer', but boiling always seemed to help incorporate all the ingredients much more consistently (especially LME which tends to clump). Using 10g/500ml of agar should create some pretty firm plates, but where those transfers were made from looks more like 5-6g/500ml (maybe it's just the pics?). What was the agar source - could there possibly something else in it?
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jcm4620
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Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: PBJ710]
#27097136 - 12/19/20 06:43 AM (3 years, 1 month ago) |
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spore prints are much much better. thats all bacteria better luck next time man
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manlord
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Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: jcm4620]
#27097891 - 12/19/20 02:47 PM (3 years, 1 month ago) |
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Yeah I hope this spore print works better, I'm assuming the grain jars are dead as well if not even 1 of the spores made it on agar. And I'm doing everything under a flowhood, and have plates sitting out with my regular agar that show no bacterial growth. I don't think my method is THAT bad to be getting contams in every plate I do a transfer to/from, when all my "test plates" show no contams. (I'd have to be messing up PRETTY hard right in front the flowhood/flame) Hoping it's the spore syringe and not my agar (the pics do look pretty light, its darker/firmer irl) but I'm going to try to put these damn syringes to some NEW, boiled agar minus the yeast and CONFIRM that it's the syringes. I'm never using syringes for agar, or proly anything else ever again, noob mistake. Thanks guys, I'll close this as soon as I confirm that my agar recipe itself wasn't causing problems i.e. causing the bacterial growth only to survive from the syringes. If anyone sees any other possible reason for this though, let me know
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PBJ710
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Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: manlord]
#27097961 - 12/19/20 03:18 PM (3 years, 1 month ago) |
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It's very possible that the syringe(s) are dirty. If the next round of plates doesn't look any better, I would try a round of PFTek jars to try to get some clone material to put on agar - wouldn't hurt to do it now if you have the materials handy. PFTek seems somewhat resistant to propagating bacterial issues.
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manlord
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Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: PBJ710]
#27097983 - 12/19/20 03:31 PM (3 years, 1 month ago) |
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Yes I've heard BRF cakes are more bacteria resistant and therefore better for syringes. Now, I've also heard that you can bulk spawn BRF cakes to a monotub. Is this correct and, if so, how many PF cakes do you think it would take to spawn a monotub? Im thinking of doing 1 PF tek run and then also do enough cakes to attempt to spawn 1 to a monotub. That way I'll have some experience with working with monotubs/get my settings dialed in before I try to run this spore print, which I'm realizing could take a couple weeks to get to me being that its almost Christmas/New Years and who knows USPS is proly dying rn so, I probably have a couple weeks (unfortunately) before I can even BEGIN to put spore print to agar. Ugh, I wish I'd known not to go with syringes, it just seemed the most noob friendly to me at first.
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ChardRich
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Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: manlord]
#27097989 - 12/19/20 03:34 PM (3 years, 1 month ago) |
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Quote:
manlord said: Just bought a spore print from a sponsor, yeah I'm hoping it's just the syringes. I have a room I use as the lab and i spray air sanitizer alot and run the flowhood regularly in there so I doubt I have a bad airborne contam proble. Like I said none of the plates I have sitting out have shown any growth at. And 65degrees? I've heard like 75-80 for incubating myc am I running too hot? I've got them at around 79 with this lava rocks electric thing and I could save money and turn that off and theyd never be below 68
Yeah don't ramp up the temps like that. 10mg LME, 10MG Agar, nothing else. PC for 45 min, let settle overnight, then inoculate or store in the fridge. 65 will be a little slower than say 72, but it will be fine. Get an inoculation loop to streak the spore print on.
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manlord
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Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: ChardRich]
#27098099 - 12/19/20 04:59 PM (3 years, 1 month ago) |
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Ok so I'm definitely about to shut this heater thing off. I'd say the room never drops below 69, I've just been fearful of temps dropping too low, (67 maybe?) and it stunting myc growth. It's normally between 71-75 in the room without the additional heating. It seems the consensus is that room temp is fine. I'm running the agar test today, going to make new agar using this recipe and see if the syringe is the issue, or my agar. Also, would you recommend streaking an MS drop or simply dropping in the middle of the plate? I have one ready for when the spore print arrives
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ChRnZN
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Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: manlord] 1
#27098285 - 12/19/20 06:49 PM (3 years, 1 month ago) |
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Streaking in a zig zag pattern yields good results.
Like so (Courtesy of Enlightenment)
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PBJ710
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Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: manlord]
#27098441 - 12/19/20 09:15 PM (3 years, 1 month ago) |
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Quote:
manlord said: Yes I've heard BRF cakes are more bacteria resistant and therefore better for syringes. Now, I've also heard that you can bulk spawn BRF cakes to a monotub. Is this correct and, if so, how many PF cakes do you think it would take to spawn a monotub? Im thinking of doing 1 PF tek run and then also do enough cakes to attempt to spawn 1 to a monotub. That way I'll have some experience with working with monotubs/get my settings dialed in before I try to run this spore print, which I'm realizing could take a couple weeks to get to me being that its almost Christmas/New Years and who knows USPS is proly dying rn so, I probably have a couple weeks (unfortunately) before I can even BEGIN to put spore print to agar. Ugh, I wish I'd known not to go with syringes, it just seemed the most noob friendly to me at first.
It's not that all syringes are dirty, it's just that many are to some degree. Some vendors do a better job of producing clean syringes than others.
You can go to bulk with BRF, but getting 4 cakes to successfully colonize will be tougher than getting 1 to with a sketchy syringe. If you can get four .5pt jars to colonize without contam, you could use that in a shoebox with 2qts of coco, but you only need a single fruit to get some clone material to put on agar. A normal monotub would need at least 12-16 HEALTHY .5pt jars, so that's probably not a wise pursuit at this point.
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manlord
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Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: PBJ710]
#27098484 - 12/19/20 10:10 PM (3 years, 1 month ago) |
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Ok, thanks for the input. Yeah thats a lot of BRF jars I doubt thats likely. But the shoebox method could be done and then like you said I could just clone to agar. I'll give it a shot while I wait for my spore print. I have some grain jars going that have just started showing either really early myc or its cobweb mold or some other white contam. I'm gonna post a picture of it tomorrow when it's hopefully a little more pronounced and would you give me your opinion on whether its myc or a white mold? About to finish my agar now, less water no yeast etc., just to make sure thats not whats causing the issue.
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manlord
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Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: ChRnZN]
#27098488 - 12/19/20 10:12 PM (3 years, 1 month ago) |
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Ok correct me if I'm wrong, but after streaking in a zigzag pattern, I'd want to watch for specific colonies to develop and then select strongest myc from multiple colonies? Cuz if I let it develop too much wouldn't the colonies like run into each other and maybe be harder to select healthy myc from the outside edges? Roughly how long after putting spores (MS or print) to agar do you typically take your first transfer? I know it varies, but. I've heard 1-2 days after you start seeing growth?
Edited by manlord (12/19/20 10:17 PM)
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PBJ710
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Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: manlord]
#27098509 - 12/19/20 10:39 PM (3 years, 1 month ago) |
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Quote:
manlord said: Ok correct me if I'm wrong, but after streaking in a zigzag pattern, I'd want to watch for specific colonies to develop and then select strongest myc from multiple colonies? Cuz if I let it develop too much wouldn't the colonies like run into each other and maybe be harder to select healthy myc from the outside edges? Roughly how long after putting spores (MS or print) to agar do you typically take your first transfer? I know it varies, but. I've heard 1-2 days after you start seeing growth?
I try to get a transfer from a spore plate when the colony is about .75" and not overlapping other colonies - take a tiny sample from the leading edge. It can take weeks to show any progress sometimes, but after growth starts showing 1-2 days is a good estimate.
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ChardRich
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Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: PBJ710]
#27099374 - 12/20/20 01:45 PM (3 years, 1 month ago) |
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Quote:
PBJ710 said:
Quote:
manlord said: Ok correct me if I'm wrong, but after streaking in a zigzag pattern, I'd want to watch for specific colonies to develop and then select strongest myc from multiple colonies? Cuz if I let it develop too much wouldn't the colonies like run into each other and maybe be harder to select healthy myc from the outside edges? Roughly how long after putting spores (MS or print) to agar do you typically take your first transfer? I know it varies, but. I've heard 1-2 days after you start seeing growth?
I try to get a transfer from a spore plate when the colony is about .75" and not overlapping other colonies - take a tiny sample from the leading edge. It can take weeks to show any progress sometimes, but after growth starts showing 1-2 days is a good estimate.
Lmao what
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manlord
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Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: PBJ710]
#27101563 - 12/21/20 07:43 PM (3 years, 1 month ago) |
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Hey could you look at these plates and tell me what you think this is? I switched up my agar recipe and getting the same result from my B+ MS syringe as all the other agar plates I've tried with this one. Watery, milky looking growth that starts after just 1-2days but doesn't look like anything I'd want to transfer.. Is this just contaminated myc? Or is the growth itself 100% contam? any suggestions guys, these plates I put MS spores on about 36hrs ago. The PE plates I did at the same time show now growth yet, and EVERY B+ plate I've made has started growing SO fast, in this exact same way. Wondering what is up here:
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PBJ710
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Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: manlord]
#27101798 - 12/21/20 10:24 PM (3 years, 1 month ago) |
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Milky looking growth on plates is usually bacteria especially if it shows up that quick - if there is any healthy growth, you should be able to transfer away from the bacteria, but it may take a few days or more to show up. Would it be possible to get some better pics of it laying flat on a light colored surface?
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manlord
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Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: PBJ710]
#27101821 - 12/21/20 10:54 PM (3 years, 1 month ago) |
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I just removed the lids off the petris in front of the flowhood for a couple seconds and took 2 pictures, hopefully these ones are better. It could just be a bacteria ridden syringe, every single plate I've made (20+) have grown just like this, milky, and very quickly. I'd reallyyyy appreciate it if you'd watch this plate over the next 1-3 days with me just so I can show you the growth and if there is ANYTHING healthy let me know and I'll transfer it asap. Just wanna make sure I'm not crazy and that this syringe is in fact the problem.
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PBJ710
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Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: manlord]
#27101934 - 12/22/20 02:25 AM (3 years, 1 month ago) |
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If they are growing that much in 36 hours, it's probably not mycelium.
What agar recipe did you use?
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manlord
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Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: PBJ710]
#27102662 - 12/22/20 03:16 PM (3 years, 1 month ago) |
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10g agar, 10g Light malt extract 400ml water Normally I use 500ml water, as well as 1g nutritional yeast. But I remade the agar with less water and no yeast to make sure it's the syringe and not my agar. Every single B+ plate has grown exactly as these 2.
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tiptrippy
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Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: manlord]
#27102698 - 12/22/20 03:46 PM (3 years, 1 month ago) |
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Unfortunately that does not look like myc either. I suggest knocking up 1 BRF half pint jar with your syringes each. Let them get to 100% colonization and wait for invitro pins. Birth them in front of your flowhood and transfer the pins to fresh agar plates.
If your sterile tek and agar recipes are done properly you should at least be getting some sort of myc growth. What do your syringes look like? Can you see any black spores in them?
Is your flowhood built properly? Have you done the lighter test? Just shooting out ideas here.
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smalltalk_canceled
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Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: manlord]
#27102756 - 12/22/20 04:33 PM (3 years, 1 month ago) |
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Make more agar, it's great practice and getting clean spawn on agar is core in the hobby. Many of my first agar plates looked horrible like this, today I'm hustling out the shrooms like magic. You'll get there, hang in there and keep making transfers.
No fingers in the juice, boii
-------------------- Willpower is the one true virtue
  
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manlord
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Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: smalltalk_canceled]
#27102811 - 12/22/20 05:18 PM (3 years, 1 month ago) |
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What possible mistakes could I be making in my agar that could cause odd growth like that? Only thing I can think that's wrong with my agar or my sterile tek etc is that my agar has some condensation on the top few lids. The bottom half or so are great, but the top 1-3 out of 10 has a bit of condensation, which ends up dripping down on the agar. However, I've got test plates out in the area my other plates are in and I havent seen any odd growth on them, no contams nothing. I wrap all plates in parafilm before they leave the flowhood area. And I only open my pressure cooker directly in front of the flowhood, agar stuffed with poly and tinfoil on top. Anything with my tek you would suggest changing? I've poured so many plates and wasted them from this syringe cuz every single one looks like this. At this point its only got like 1cc left in it and its not nearly as thick with spores as my PE syringe, but I can still see some in there. Maybe its just bacterial, I will know once this PE starts to germinate, hopefully it's healthy and then I know my agar is ok etc. Ill post some pics of these PE plates
Edited by manlord (12/22/20 05:25 PM)
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manlord
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Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: tiptrippy]
#27102815 - 12/22/20 05:23 PM (3 years, 1 month ago) |
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The flowhood is a Bonzai flowhood from an online vendor and comes with a warranty etc. so I hope it works well, haven't tried the lighter test whats that? It def prevents a lighter from lighting within a couple ft of it when it's on. I have it in a separate room, and non of my "test plates" have shown signs of contam. Only once I put this MS syringe to them, I also retried with PE and they didn't start showing growth so suspiciously quickly like the b+ have been (every time on like 20+ plates). So I have more hope for the PE, its been 48hrs and I can just barely see some growth on the PE plates, im gonna post pics soon so you guys can hopefully take a look for me. If I can isolate anything healthy, I will, but would rather ask you guys first. I have some grain jars showing healthy looking growth rn and have a spore print on the way, if I haveee to I'll do BRF cakes, almost outta syringe now
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smalltalk_canceled
Babnik


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Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: manlord]
#27102831 - 12/22/20 05:35 PM (3 years, 1 month ago) |
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You are working from syringes. They are always garbage imo Just a step towards clean agar If you have a flowhood and a clue, it points towards the syringes.
Are all germination from the syringe like this
-------------------- Willpower is the one true virtue
  
Edited by smalltalk_canceled (12/22/20 05:35 PM)
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manlord
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Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: smalltalk_canceled]
#27102845 - 12/22/20 05:44 PM (3 years, 1 month ago) |
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Yeah, from this syringe, every plate germinates exactly like these. All milky and crazy and they take off way too fast. I should have kept my other syringes until I got a good agar plate to transfer from but I didn't really think about the possibility that the entire syringe is contamed af. Hopefully the PE will give me something to start on agar thats healthy, cuz all the other syringes I put to grain already, a few of which are looking like healthy myc growth so I could always g2g if they make it
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smalltalk_canceled
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Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: manlord] 1
#27102867 - 12/22/20 05:57 PM (3 years, 1 month ago) |
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One trick you could try is to use the last drops of the syringe on straight pced 2,5h grains and try to isolate from the first myc growth, back to agar. Work from that
Since this looks like bacteria, it may help. There's a long explanation why but I'd rather get more high
-------------------- Willpower is the one true virtue
  
Edited by smalltalk_canceled (12/22/20 05:59 PM)
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PBJ710
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Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: smalltalk_canceled]
#27102940 - 12/22/20 06:46 PM (3 years, 1 month ago) |
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I second tiptrippy's recommendation of BRF cakes for less than ideal syringes - BRF is somewhat resistant to bacterial growth and can be a reliable way of getting clone material.
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manlord
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Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: smalltalk_canceled]
#27103432 - 12/23/20 02:39 AM (3 years, 1 month ago) |
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How long do I have to let grain colonize before selecting a piece to put to agar? I see myc growth on my jars now, but it's not really mature myc yet. There are grains that are all white and colonized though, should I maybe wait until I do the 1/3rd shake? Or grab it asap cuz it's healthy?
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tiptrippy
The Mechanic



Registered: 09/09/20
Posts: 1,131
Loc: United States
Last seen: 1 year, 6 months
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Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: manlord]
#27103440 - 12/23/20 02:57 AM (3 years, 1 month ago) |
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Quote:
manlord said: How long do I have to let grain colonize before selecting a piece to put to agar? I see myc growth on my jars now, but it's not really mature myc yet. There are grains that are all white and colonized though, should I maybe wait until I do the 1/3rd shake? Or grab it asap cuz it's healthy?
You could grab a single grain as soon as it germinated and colonized 1 grain. But if you open a grain jar you take the chance of contaminating the whole jar.
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HappinessStan
Fungivore



Registered: 10/10/12
Posts: 1,617
Loc: Worcester, UK
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Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: manlord]
#27103461 - 12/23/20 03:17 AM (3 years, 1 month ago) |
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I had the same issues as you with an MS syringe. I saw someone here say to try brf pucks and that worked for me, spores germinated in a week and I just pulled some mycelium off in my SAB and put that to agar plates to clean up. My little pucks are now fully colonised and I'm waiting for pins to clone and hopefully a cap to make a print for future agar use. MS syringes are always dirty and the bacteria will germinate befote the mycelium; go to brf in sterilised jars, it works.
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jcm4620
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Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: HappinessStan]
#27103467 - 12/23/20 03:24 AM (3 years, 1 month ago) |
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i think that syringe is so fucked even goin to grains then to agar may not work. im sure someone here can send him a cube print?? id do it but im out and aint grown a cube in a year n a hlf. but what i will do is if someone wants to send him a cube print il send you a pan print
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tiptrippy
The Mechanic



Registered: 09/09/20
Posts: 1,131
Loc: United States
Last seen: 1 year, 6 months
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Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: jcm4620]
#27103809 - 12/23/20 09:39 AM (3 years, 1 month ago) |
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OP shoot me a PM I'll send you some goodies.
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manlord
Stranger


Registered: 12/18/20
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Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: tiptrippy]
#27104642 - 12/23/20 05:55 PM (3 years, 1 month ago) |
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Thank you so much for the help guys. I'm gonna do a buckshot approach here, and I'll go ahead and risk 1 jar (consider it contamed) and put 1 piece of the healthiest looking myc to agar and see. I'll ALSO make some BRF cakes, I think all I need is some actual BRF, and put the very last of this B+ syringe to it and see what happens. THANK you Trippy, JCM and Stan for the help, much appreciated guys. I'll close this out shortly and repost any other questions I have regarding my BRF cakes, grain jars, or anything agar. You guys have been a huge help for a noob, MUCH appreciated!
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tiptrippy
The Mechanic



Registered: 09/09/20
Posts: 1,131
Loc: United States
Last seen: 1 year, 6 months
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Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: manlord]
#27106764 - 12/25/20 12:34 AM (3 years, 1 month ago) |
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Goodiebag is on the way. Those should give you a huge jump start. Keep us posted with your grows and good luck!!
Mush love 🍄💚💚
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jcm4620
Stranger


Registered: 05/26/19
Posts: 6,700
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Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: tiptrippy]
#27106767 - 12/25/20 12:39 AM (3 years, 1 month ago) |
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trippy shoot me an address and il send ya a pan print😃 thanks for helpn out a fellow member 👍👍
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tiptrippy
The Mechanic



Registered: 09/09/20
Posts: 1,131
Loc: United States
Last seen: 1 year, 6 months
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Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: jcm4620]
#27106820 - 12/25/20 02:27 AM (3 years, 1 month ago) |
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Quote:
jcm4620 said: trippy shoot me an address and il send ya a pan print😃 thanks for helpn out a fellow member 👍👍
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