Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Capsules   Kraken Kratom Red Vein Kratom   North Spore Bulk Substrate   Bridgetown Botanicals CBD Concentrates   Original Sensible Seeds Bulk Cannabis Seeds   PhytoExtractum Kratom Powder for Sale   Mushroom-Hut Liquid Cultures

Jump to first unread post Pages: 1 | 2 | 3 | Next >  [ show all ]
Offlinemanlord
Stranger
 User Gallery

Registered: 12/18/20
Posts: 153
Last seen: 1 year, 4 months
1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? *DELETED*
    #27096582 - 12/18/20 08:06 PM (3 years, 1 month ago)

Post deleted by manlord

Reason for deletion: makes me look like huge noob


Edited by manlord (12/18/20 10:40 PM)


Extras: Filter Print Post Top
Offlinebw86
Doesn't play well with others


Registered: 11/12/06
Posts: 5,941
Loc: 7b
Last seen: 14 hours, 42 minutes
Trusted Cultivator
Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: manlord]
    #27096622 - 12/18/20 08:31 PM (3 years, 1 month ago)

Unfortunately there is no cubensis mycelium in any of those plates you have to look up better pictures.


Extras: Filter Print Post Top
InvisibleChRnZN
Din of Doom
 Unread Journal User Gallery

Registered: 12/21/08
Posts: 6,265
Loc: ADK
Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: bw86]
    #27096624 - 12/18/20 08:33 PM (3 years, 1 month ago)

:whathesaid: Mycelium should be thready


Extras: Filter Print Post Top
Offlinemanlord
Stranger
 User Gallery

Registered: 12/18/20
Posts: 153
Last seen: 1 year, 4 months
Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: ChRnZN]
    #27096638 - 12/18/20 08:42 PM (3 years, 1 month ago)

What about the PE transfers? I mean you can def see thready growth on the wedges I transferred in the center, but its not leading to better growth. Do you think I got messed up syringes, or what would lead to that watery looking myc? Is every plate a contam, possibly every syringe I got was contam? I mean Idk if everything on my plates is contam, I can clearly see the growth after I add spores, and none of my other plates I have just sitting out are contaminating.
The PE showed some ropeyness, I know its hard to tell from the pics, but I transferred the more ropey parts but it hasn't led to more rhizo growth so far.  The B+ I'm lost on, how could multiple syringes produce the exact same looking contam, if thats what it is, on like 10 different plates? Is my agar recipe too watery, idk what could be causing this. Any ideas on casusation?


Extras: Filter Print Post Top
Offlinemanlord
Stranger
 User Gallery

Registered: 12/18/20
Posts: 153
Last seen: 1 year, 4 months
Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: ChRnZN]
    #27096652 - 12/18/20 08:50 PM (3 years, 1 month ago)

Yeah I feel you! I realize this is unhealthy looking myc, hence the post. I'm looking for ANY possible reason this could be happening to like 20 plates, no signs of contam in blank plates left out at same location.
Could both of the 2 B+ syringes I used be carrying a similar contam thats infecting each plate? Idk sounds unlikely but could be the case. I'm more thinking that this is just reallyy unhealthy myc or something. Should I try a spore print and assume these MS syringes are trash? I'm just wondering what went wrong here


Extras: Filter Print Post Top
InvisibleChRnZN
Din of Doom
 Unread Journal User Gallery

Registered: 12/21/08
Posts: 6,265
Loc: ADK
Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: manlord]
    #27096669 - 12/18/20 09:00 PM (3 years, 1 month ago)

The problem could be bacterial contamination in the water from the syringes. I like spore prints myself. Freezing them before usage decreases contamination. Make sure your environment is clean. Lots of mycelium killers are floating around us all the time. Concerning your jars' placement - a shelf by the sun at 65 F would be best.


Extras: Filter Print Post Top
Offlinemanlord
Stranger
 User Gallery

Registered: 12/18/20
Posts: 153
Last seen: 1 year, 4 months
Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: ChRnZN]
    #27096691 - 12/18/20 09:12 PM (3 years, 1 month ago)

Just bought a spore print from a sponsor, yeah I'm hoping it's just the syringes. I have a room I use as the lab and i spray air sanitizer alot and run the flowhood regularly in there so I doubt I have a bad airborne contam proble. Like I said none of the plates I have sitting out have shown any growth at.
And 65degrees? I've heard like 75-80 for incubating myc am I running too hot? I've got them at around 79 with this lava rocks electric thing and I could save money and turn that off and theyd never be below 68


Extras: Filter Print Post Top
OfflinePBJ710
Strangler

Folding@home Statistics
Registered: 07/05/19
Posts: 2,623
Last seen: 1 month, 5 days
Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: manlord]
    #27096819 - 12/18/20 10:41 PM (3 years, 1 month ago)

Are you boiling the water before adding your LME/Agar powder?  The agar itself looks really soft in the first couple pics.  I would ditch the yeast at least temporarily to see how it effects the agar.  Not sure what a 'lava rocks electric thing' is, but you don't need any heaters as long as you are comfortable in the room.  If you have to use a heater, make sure that it's indirect heating or it can fry your mycelium.


Extras: Filter Print Post Top
Offlinemanlord
Stranger
 User Gallery

Registered: 12/18/20
Posts: 153
Last seen: 1 year, 4 months
Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: PBJ710]
    #27096840 - 12/18/20 10:52 PM (3 years, 1 month ago)

No, I haven't boiled the water before adding the agar/malt ex. Does that help? I'll try that and ditch the yeast maybe it's the agar, that would make sense why every plate is off and why the spores seem so watery/runny. I'm thinking it's got to be either the agar itself or the spore syringes, or both.
And the lava rocks thing is just a really small electric heater that lightly heats up a little pan of lava rocks which generates a small amount of heat. The jars are a far enough away where its not like direct heat, just ups the temp a few degrees in the room. But if not necessary, I'll save the money and shut it off to save $.


Extras: Filter Print Post Top
OfflinePBJ710
Strangler

Folding@home Statistics
Registered: 07/05/19
Posts: 2,623
Last seen: 1 month, 5 days
Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: manlord]
    #27097125 - 12/19/20 06:31 AM (3 years, 1 month ago)

Not 100% sure that it's 'the answer', but boiling always seemed to help incorporate all the ingredients much more consistently (especially LME which tends to clump).  Using 10g/500ml of agar should create some pretty firm plates, but where those transfers were made from looks more like 5-6g/500ml (maybe it's just the pics?).  What was the agar source - could  there possibly something else in it?


Extras: Filter Print Post Top
Offlinejcm4620
Stranger
 User Gallery

Registered: 05/26/19
Posts: 6,700
Last seen: 2 years, 1 day
Trusted Cultivator
Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: PBJ710]
    #27097136 - 12/19/20 06:43 AM (3 years, 1 month ago)

spore prints are much much better. thats all bacteria better luck next time man


--------------------
PANAEOLUS FRUITING MADE SIMPLE



Extras: Filter Print Post Top
Offlinemanlord
Stranger
 User Gallery

Registered: 12/18/20
Posts: 153
Last seen: 1 year, 4 months
Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: jcm4620]
    #27097891 - 12/19/20 02:47 PM (3 years, 1 month ago)

Yeah I hope this spore print works better, I'm assuming the grain jars are dead as well if not even 1 of the spores made it on agar. And I'm doing everything under a flowhood, and have plates sitting out with my regular agar that show no bacterial growth. I don't think my method is THAT bad to be getting contams in every plate I do a transfer to/from, when all my "test plates" show no contams. (I'd have to be messing up PRETTY hard right in front the flowhood/flame)
  Hoping it's the spore syringe and not my agar (the pics do look pretty light, its darker/firmer irl) but I'm going to try to put these damn syringes to some NEW, boiled agar minus the yeast and CONFIRM that it's the syringes.
I'm never using syringes for agar, or proly anything else ever again, noob mistake.
  Thanks guys, I'll close this as soon as I confirm that my agar recipe itself wasn't causing problems i.e. causing the bacterial growth only to survive from the syringes. If anyone sees any other possible reason for this though, let me know


Extras: Filter Print Post Top
OfflinePBJ710
Strangler

Folding@home Statistics
Registered: 07/05/19
Posts: 2,623
Last seen: 1 month, 5 days
Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: manlord]
    #27097961 - 12/19/20 03:18 PM (3 years, 1 month ago)

It's very possible that the syringe(s) are dirty.  If the next round of plates doesn't look any better, I would try a round of PFTek jars to try to get some clone material to put on agar - wouldn't hurt to do it now if you have the materials handy.  PFTek seems somewhat resistant to propagating bacterial issues.


Extras: Filter Print Post Top
Offlinemanlord
Stranger
 User Gallery

Registered: 12/18/20
Posts: 153
Last seen: 1 year, 4 months
Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: PBJ710]
    #27097983 - 12/19/20 03:31 PM (3 years, 1 month ago)

Yes I've heard BRF cakes are more bacteria resistant and therefore better for syringes. Now, I've also heard that you can bulk spawn BRF cakes to a monotub. Is this correct and, if so, how many PF cakes do you think it would take to spawn a monotub?
Im thinking of doing 1 PF tek run and then also do enough cakes to attempt to spawn 1 to a monotub. That way I'll have some experience with working with monotubs/get my settings dialed in before I try to run this spore print, which I'm realizing could take a couple weeks to get to me being that its almost Christmas/New Years and who knows USPS is proly dying rn so, I probably have a couple weeks (unfortunately) before I can even BEGIN to put spore print to agar. Ugh, I wish I'd known not to go with syringes, it just seemed the most noob friendly to me at first.


Extras: Filter Print Post Top
OfflineChardRich
chardzard
I'm a teapot User Gallery


Registered: 04/16/19
Posts: 1,167
Loc: Upper Left USA
Last seen: 1 year, 24 days
Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: manlord]
    #27097989 - 12/19/20 03:34 PM (3 years, 1 month ago)

Quote:

manlord said:
Just bought a spore print from a sponsor, yeah I'm hoping it's just the syringes. I have a room I use as the lab and i spray air sanitizer alot and run the flowhood regularly in there so I doubt I have a bad airborne contam proble. Like I said none of the plates I have sitting out have shown any growth at.
And 65degrees? I've heard like 75-80 for incubating myc am I running too hot? I've got them at around 79 with this lava rocks electric thing and I could save money and turn that off and theyd never be below 68




Yeah don't ramp up the temps like that. 10mg LME, 10MG Agar, nothing else. PC for 45 min, let settle overnight, then inoculate or store in the fridge. 65 will be a little slower than say 72, but it will be fine. Get an inoculation loop to streak the spore print on.


--------------------



Extras: Filter Print Post Top
Offlinemanlord
Stranger
 User Gallery

Registered: 12/18/20
Posts: 153
Last seen: 1 year, 4 months
Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: ChardRich]
    #27098099 - 12/19/20 04:59 PM (3 years, 1 month ago)

Ok so I'm definitely about to shut this heater thing off. I'd say the room never drops below 69, I've just been fearful of temps dropping too low, (67 maybe?) and it stunting myc growth. It's normally between 71-75 in the room without the additional heating. It seems the consensus is that room temp is fine.
I'm running the agar test today, going to make new agar using this recipe and see if the syringe is the issue, or my agar.
Also, would you recommend streaking an MS drop or simply dropping in the middle of the plate? I have one ready for when the spore print arrives


Extras: Filter Print Post Top
InvisibleChRnZN
Din of Doom
 Unread Journal User Gallery

Registered: 12/21/08
Posts: 6,265
Loc: ADK
Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: manlord] * 1
    #27098285 - 12/19/20 06:49 PM (3 years, 1 month ago)

Streaking in a zig zag pattern yields good results.

Like so (Courtesy of Enlightenment)


Extras: Filter Print Post Top
OfflinePBJ710
Strangler

Folding@home Statistics
Registered: 07/05/19
Posts: 2,623
Last seen: 1 month, 5 days
Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: manlord]
    #27098441 - 12/19/20 09:15 PM (3 years, 1 month ago)

Quote:

manlord said:
Yes I've heard BRF cakes are more bacteria resistant and therefore better for syringes. Now, I've also heard that you can bulk spawn BRF cakes to a monotub. Is this correct and, if so, how many PF cakes do you think it would take to spawn a monotub?
Im thinking of doing 1 PF tek run and then also do enough cakes to attempt to spawn 1 to a monotub. That way I'll have some experience with working with monotubs/get my settings dialed in before I try to run this spore print, which I'm realizing could take a couple weeks to get to me being that its almost Christmas/New Years and who knows USPS is proly dying rn so, I probably have a couple weeks (unfortunately) before I can even BEGIN to put spore print to agar. Ugh, I wish I'd known not to go with syringes, it just seemed the most noob friendly to me at first.




It's not that all syringes are dirty, it's just that many are to some degree.  Some vendors do a better job of producing clean syringes than others.

You can go to bulk with BRF, but getting 4 cakes to successfully colonize will be tougher than getting 1 to with a sketchy syringe.  If you can get four .5pt jars to colonize without contam, you could use that in a shoebox with 2qts of coco, but you only need a single fruit to get some clone material to put on agar.  A normal monotub would need at least 12-16 HEALTHY .5pt jars, so that's probably not a wise pursuit at this point.


Extras: Filter Print Post Top
Offlinemanlord
Stranger
 User Gallery

Registered: 12/18/20
Posts: 153
Last seen: 1 year, 4 months
Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: PBJ710]
    #27098484 - 12/19/20 10:10 PM (3 years, 1 month ago)

Ok, thanks for the input. Yeah thats a lot of BRF jars I doubt thats likely. But the shoebox method could be done and then like you said I could just clone to agar. I'll give it a shot while I wait for my spore print.
I have some grain jars going that have just started showing either really early myc or its cobweb mold or some other white contam. I'm gonna post a picture of it tomorrow when it's hopefully a little more pronounced and would you give me your opinion on whether its myc or a white mold? About to finish my agar now, less water no yeast etc., just to make sure thats not whats causing the issue.


Extras: Filter Print Post Top
Offlinemanlord
Stranger
 User Gallery

Registered: 12/18/20
Posts: 153
Last seen: 1 year, 4 months
Re: 1st Time working w/ Agar, Weak, Watery looking Myc from MS syringes? [Re: ChRnZN]
    #27098488 - 12/19/20 10:12 PM (3 years, 1 month ago)

Ok correct me if I'm wrong, but after streaking in a zigzag pattern, I'd want to watch for specific colonies to develop and then select strongest myc from multiple colonies? Cuz if I let it develop too much wouldn't the colonies like run into each other and maybe be harder to select healthy myc from the outside edges? Roughly how long after putting spores (MS or print) to agar do you typically take your first transfer? I know it varies, but. I've heard 1-2 days after you start seeing growth?


Edited by manlord (12/19/20 10:17 PM)


Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 | 3 | Next >  [ show all ]

Shop: Left Coast Kratom Buy Kratom Capsules   Kraken Kratom Red Vein Kratom   North Spore Bulk Substrate   Bridgetown Botanicals CBD Concentrates   Original Sensible Seeds Bulk Cannabis Seeds   PhytoExtractum Kratom Powder for Sale   Mushroom-Hut Liquid Cultures


Similar ThreadsPosterViewsRepliesLast post
* Agar Question TerribleInstict 1,381 4 11/10/01 11:40 PM
by anima
* Agar....Re-using question Erbaliza 1,272 8 12/02/02 06:26 AM
by rhizo
* 1st Liquid Culture, arrrgh! Seril 14,346 10 02/28/06 08:35 PM
by Limelight_Liver
* Agar and growing that white stuff! pj541 2,517 5 10/15/01 04:06 PM
by pj541
* 1st cake hit by green, & caseing sterility.... hyper_dermic 1,273 5 10/24/02 08:06 AM
by hyper_dermic
* Re: Dose Hip's bulk tek work? Anonymous 591 2 04/13/00 07:47 PM
by Hippie3
* 1st time casing strategy........ bruisedBlue 1,453 8 10/06/02 06:42 PM
by SixTango
* the point of agar is? upupup 875 3 11/23/01 04:23 PM
by kingkc

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
756 topic views. 12 members, 90 guests and 30 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.05 seconds spending 0.008 seconds on 13 queries.