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Offlinemaindex
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Registered: 09/18/20
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1st grow, 1st agar, 1st transfer, 1st post. Contamination?
    #27095826 - 12/18/20 01:11 PM (3 years, 1 month ago)

Hi everybody
Long time reader first time poster here.

Please take a look at my plates. I guess I fucked up during transfer.
(I did spore->agar from my own GT print and first plates looked good and clean to me)
I'm afraid some of them are contaminated now. 
Can someone verify this for me?






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OfflineSpaceDawg
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Re: 1st grow, 1st agar, 1st transfer, 1st post. Contamination? [Re: maindex]
    #27095852 - 12/18/20 01:36 PM (3 years, 1 month ago)

some of them do look like they have some bacteria. first, third, and fourth plate still look clean.


--------------------
I must not fear.
Fear is the mind-killer.
Fear is the little-death that brings total obliteration.
I will face my fear.
I will permit it to pass over me and through me.
And when it has gone past I will turn the inner eye to see its path.
Where the fear has gone there will be nothing.
Only I will remain.


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Offlinemaindex
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Re: 1st grow, 1st agar, 1st transfer, 1st post. Contamination? [Re: SpaceDawg]
    #27095879 - 12/18/20 01:53 PM (3 years, 1 month ago)

well thank you. I was looking at this fuzzy part on 4th plate between 10 and 12 o'clock but I guess it just different type mycelium.
I really like leggy ones on the last though


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OfflineSpaceDawg
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Re: 1st grow, 1st agar, 1st transfer, 1st post. Contamination? [Re: maindex]
    #27095916 - 12/18/20 02:20 PM (3 years, 1 month ago)

yeah the fuzzy mycelium is still fine. the rhizomorphic stuff is just more desirable imo.

all your plates look pretty good anyway. there's no mold or anything really nasty just a bit of bacteria.

you could transfer that section from the last plate if you like.


--------------------
I must not fear.
Fear is the mind-killer.
Fear is the little-death that brings total obliteration.
I will face my fear.
I will permit it to pass over me and through me.
And when it has gone past I will turn the inner eye to see its path.
Where the fear has gone there will be nothing.
Only I will remain.


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Offlinemaindex
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Registered: 09/18/20
Posts: 3
Last seen: 2 years, 8 months
Re: 1st grow, 1st agar, 1st transfer, 1st post. Contamination? [Re: maindex]
    #27095972 - 12/18/20 02:51 PM (3 years, 1 month ago)

well that's a relief    :dancing: 
i was kinda planning go from this to LI->grain but maybe one extra transfer wouldn't hurt.


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OfflineSpaceDawg
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Last seen: 2 months, 13 days
Re: 1st grow, 1st agar, 1st transfer, 1st post. Contamination? [Re: maindex]
    #27095980 - 12/18/20 02:59 PM (3 years, 1 month ago)

if you wanted to use plates 2 and 5 id transfer again.

just better to make sure the culture is 100% clean before making LI with it ya know?

all other ones would be good to use for LI imo.

best of luck.


--------------------
I must not fear.
Fear is the mind-killer.
Fear is the little-death that brings total obliteration.
I will face my fear.
I will permit it to pass over me and through me.
And when it has gone past I will turn the inner eye to see its path.
Where the fear has gone there will be nothing.
Only I will remain.


Extras: Filter Print Post Top
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