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OfflineGwindor
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How many agar transfers prior to grain spawn?
    #27095259 - 12/18/20 04:17 AM (3 years, 1 month ago)

How many agar transfers is it better to make before going to grain spawn stage?
As I understood the main point of this agar transfers from petri to petri dishes - to isolate monospore culture. And monospore culture will give more prolific and unified yields with fruits covering all the surface of substrate and fruits will be equal heights.
How many transfers do we need to make to be sure that it’s monospore in petri dish now and not the multispore?


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Offlinejcm4620
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Re: How many agar transfers prior to grain spawn? [Re: Gwindor] * 3
    #27095278 - 12/18/20 04:50 AM (3 years, 1 month ago)

u got some terms messed  in there but i try to get on grains as fast as absolutely possible. il streak a plate then il transfer the germination points i want to new plates to let them grow out about the size of a nickle or a qtr. then il take a cpl transfers from there to new plates and let them grow out to about the  size of a qtr and as long as those look good and r clean they r goin to grain. im after clones anyway so i want my initial gene pool to choose from as wide as possible. just cuz a plate looks pretty and round dont mean jack shit. and if u ask me too many ppl spend way too much time on agar in the begining when they should be goin to grain. the problem with spending too much time on agar is that you could be transfering away from something you really want without even knowing it. now as far as the fruits all growing even and at the same pace. ur gnna have more of that with a cloned culture vs a ms culture. just cuz ur taking a bunch of transfers it doesent change the fact that its still a ms culture. the term monoculture is used and thrown around too loosely at times and gets mis used


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PANAEOLUS FRUITING MADE SIMPLE



Edited by jcm4620 (12/18/20 04:52 AM)


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OfflineGwindor
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Re: How many agar transfers prior to grain spawn? [Re: jcm4620]
    #27095284 - 12/18/20 04:54 AM (3 years, 1 month ago)

Thanks. I've watched Roger video about agar isolation transfers. And he told in video that usually it's ok to perform 4-5 transfers to get some kind of "great" culture before proceed to grain.
I'm not hurrying at all actually.


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Re: How many agar transfers prior to grain spawn? [Re: jcm4620]
    #27095289 - 12/18/20 04:59 AM (3 years, 1 month ago)

:whathesaid:


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Offlinejcm4620
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Re: How many agar transfers prior to grain spawn?qq [Re: Shroomysamba] * 2
    #27095343 - 12/18/20 06:10 AM (3 years, 1 month ago)

a great culture lol its not possible to know what anything is though till u fruit it out. just cuz its round pretty and perfectly symetrical dont mean squat. iv had plenty of cultures that looked stellar turn out to be useless trash and ive had en equal amount of shitty or average lookin cultures kick absolute ass. its a crap shoot and there is no way of knowing  other than fruiting. but like i said i prefer to be on grains by t2 or t3 is all. now it dont mean that its gnna hurt anything by goin to t4 or t5 especially if ur fruiting out every one but i just prefer not doin all that extra agar work if i dont have to is all


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OfflineGwindor
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Re: How many agar transfers prior to grain spawn?qq [Re: jcm4620]
    #27095400 - 12/18/20 07:45 AM (3 years, 1 month ago)

Quote:

jcm4620 said:
i prefer to be on grains by t2 or t3 is all. now it dont mean that its gnna hurt anything by goin to t4 or t5 especially if ur fruiting out every one but i just prefer not doin all that extra agar work if i dont have to is all



Thank you.
Another question. Do I need to put just 1 piece of colonized agar to grain jar? Or several pieces from the same petri dish? I mean, if there'll be several pieces maybe there'll be kind of "multispore" in grain spawn? And we're trying to get monospore.
How do you do this? You put 1 agar wedge or several?


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Offlinejcm4620
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Re: How many agar transfers prior to grain spawn?qq [Re: Gwindor] * 2
    #27095500 - 12/18/20 09:04 AM (3 years, 1 month ago)

first off dont take this as an insult. but i really think u need to go back and learn the correct terms. as ur just gnna spread confusion to others who dont know better that may see this  and  also you will confuse yourself in the process. u need to learn about how fungi live thru their life cycle and how theyreproduce. also learn about what a monoculture and a multispore inoculation is. u can have 1 wedge or 5 it dont matter they r both a multispore inoculation. while i think i know what ur saying ur just saying it wrong is all. learning the correct terms will only help u understand everything much better.


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Offlinejcm4620
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Re: How many agar transfers prior to grain spawn?qq [Re: jcm4620] * 2
    #27095515 - 12/18/20 09:16 AM (3 years, 1 month ago)



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OfflineEugene Gesuale
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Re: How many agar transfers prior to grain spawn? [Re: jcm4620] * 1
    #27095612 - 12/18/20 10:32 AM (3 years, 1 month ago)

Quote:

jcm4620 said:
u got some terms messed  in there but i try to get on grains as fast as absolutely possible. il streak a plate then il transfer the germination points i want to new plates to let them grow out about the size of a nickle or a qtr. then il take a cpl transfers from there to new plates and let them grow out to about the  size of a qtr and as long as those look good and r clean they r goin to grain. im after clones anyway so i want my initial gene pool to choose from as wide as possible. just cuz a plate looks pretty and round dont mean jack shit. and if u ask me too many ppl spend way too much time on agar in the begining when they should be goin to grain. the problem with spending too much time on agar is that you could be transfering away from something you really want without even knowing it. now as far as the fruits all growing even and at the same pace. ur gnna have more of that with a cloned culture vs a ms culture. just cuz ur taking a bunch of transfers it doesent change the fact that its still a ms culture. the term monoculture is used and thrown around too loosely at times and gets mis used



One way I though of to ensure you don’t transfer away desirable traits, and this is just a theory, would be to take T1 T2 T3 T4 T5 T6 T7 and T8 of a successful cross or clone you like and drop them all to the same grain. Wouldn’t they compete? Leaving only the best phenos to fruit? It feels like reverse engineering.


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Offlinejcm4620
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Re: How many agar transfers prior to grain spawn? [Re: Eugene Gesuale] * 1
    #27095645 - 12/18/20 10:55 AM (3 years, 1 month ago)

when i get a clone fir instance my jambo culture what il do is take a clone that i feel has promise and i will take as many transfers as i can possibly take from that master plate. all of which will be fruited out on their own. but i always have a master plate of each one i fruit so if its a good one i can go back to it and once i find my absolute best one(s) out of that il then take as many transfers as i can from those and repeat the process. i do that until i get it down to the best possible fruiter that i have then thats the one that gets slanted and saved. sometimes ul get lucky and find that killer off the bat but more often than not u gtta work at it. and the best way to do that is thru many many small sepperate controlled grows


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InvisibleVinci
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Re: How many agar transfers prior to grain spawn? [Re: Eugene Gesuale]
    #27095646 - 12/18/20 10:56 AM (3 years, 1 month ago)

Quote:

Adrenochrome said:
Quote:

jcm4620 said:
u got some terms messed  in there but i try to get on grains as fast as absolutely possible. il streak a plate then il transfer the germination points i want to new plates to let them grow out about the size of a nickle or a qtr. then il take a cpl transfers from there to new plates and let them grow out to about the  size of a qtr and as long as those look good and r clean they r goin to grain. im after clones anyway so i want my initial gene pool to choose from as wide as possible. just cuz a plate looks pretty and round dont mean jack shit. and if u ask me too many ppl spend way too much time on agar in the begining when they should be goin to grain. the problem with spending too much time on agar is that you could be transfering away from something you really want without even knowing it. now as far as the fruits all growing even and at the same pace. ur gnna have more of that with a cloned culture vs a ms culture. just cuz ur taking a bunch of transfers it doesent change the fact that its still a ms culture. the term monoculture is used and thrown around too loosely at times and gets mis used



One way I though of to ensure you don’t transfer away desirable traits, and this is just a theory, would be to take T1 T2 T3 T4 T5 T6 T7 and T8 of a successful cross or clone you like and drop them all to the same grain. Wouldn’t they compete? Leaving only the best phenos to fruit? It feels like reverse engineering.




I usually do the clone hunt you both describe although a homie of mine in the tribe swears by just transferring 3-4 times until there's no weak sections in the cultures, make LC, test them all. Expand the best LC and use that. This cuts out a fruiting cycle so your cultures will behave younger as less senescence has occurred. It's also a faster path to having slave and master LCs of your end culture if you go this route and use half the T3/T4 plate to noc a master LC and the other half to grain for the test. Homie's cultures are damn impressive.


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Offlinejcm4620
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Re: How many agar transfers prior to grain spawn? [Re: Vinci] * 1
    #27095658 - 12/18/20 11:01 AM (3 years, 1 month ago)

i just dont like to judge a potential keeper or a trasher by looks alone as i said ive had shit that i thought looked sorry as hell and ended up bein insanely awsum so ots hard for me to not fruit them all just cuz that lil voice in my head always says well what ifπŸ˜‚πŸ˜‚


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Offlinejcm4620
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Re: How many agar transfers prior to grain spawn? [Re: jcm4620]
    #27095662 - 12/18/20 11:05 AM (3 years, 1 month ago)

i actually used to use plastic solo cups cut down for fruiting out test cultures so im talkn about each one in the same substrate at the same time in the same environment and in the same size container. that way i can keep everything but the culture the same. doin it that way aint that bad and u can find ir good ones pretty fast


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OfflineEugene Gesuale
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Re: How many agar transfers prior to grain spawn? [Re: jcm4620]
    #27095674 - 12/18/20 11:10 AM (3 years, 1 month ago)

I like this


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InvisibleVinci
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Re: How many agar transfers prior to grain spawn? [Re: Eugene Gesuale]
    #27095720 - 12/18/20 11:41 AM (3 years, 1 month ago)

I like the mini sub idea, why'd you stop jcm?


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Offlinejcm4620
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Re: How many agar transfers prior to grain spawn? [Re: Vinci]
    #27095735 - 12/18/20 11:48 AM (3 years, 1 month ago)

il still do it when im looking for keepers but i havent dove into a new project lately yet. i did give it a go with some rdu back in july and august but had issues with retarded genetics and then i ended up shutting down to clean and then got caught up in home repair work. but i got new lil containers to use now instead of the cups. i just never posted any of that shit as its just tedious n small and there is too much of it. but i do have another project in mind for my next one with jakes nec d

which il be doing after this run of my jambos r done


Edited by jcm4620 (12/18/20 11:49 AM)


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OfflineEugene Gesuale
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Re: How many agar transfers prior to grain spawn? [Re: jcm4620]
    #27095773 - 12/18/20 12:15 PM (3 years, 1 month ago)

Has anybody used any gene editing tools equipment to play around with the DNA


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Offlinejcm4620
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Re: How many agar transfers prior to grain spawn? [Re: Eugene Gesuale]
    #27095779 - 12/18/20 12:20 PM (3 years, 1 month ago)

im not aware of anything other than pcr testing. not sure🀷🏼


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Re: How many agar transfers prior to grain spawn? [Re: jcm4620]
    #27096316 - 12/18/20 06:00 PM (3 years, 1 month ago)

Got any links to a similar tek for these cup grows if not I’d love to see it if you ever get around to it or have time to explain more please pm me  :thanx:


Edited by Shroomysamba (12/18/20 06:02 PM)


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Re: How many agar transfers prior to grain spawn? [Re: Shroomysamba] * 1
    #27096334 - 12/18/20 06:07 PM (3 years, 1 month ago)

once I'm confident it's clean I shoot for 3 or 4 more transfers


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I'm tired do me a favor


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Offlinejcm4620
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Re: How many agar transfers prior to grain spawn? [Re: cronicr]
    #27096733 - 12/18/20 09:39 PM (3 years, 1 month ago)

FUCKIN CRON 🀘🀘🀘 what up man long time no c


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OfflinecronicrFacebook
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Re: How many agar transfers prior to grain spawn? [Re: jcm4620]
    #27096759 - 12/18/20 10:00 PM (3 years, 1 month ago)

hey fam I'm doing alright bruthahows life treating ya


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Offlinejcm4620
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Re: How many agar transfers prior to grain spawn? [Re: cronicr]
    #27096762 - 12/18/20 10:03 PM (3 years, 1 month ago)

oh u know just livin in a covid prison state lol πŸ˜‚


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OfflinecronicrFacebook
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Re: How many agar transfers prior to grain spawn? [Re: jcm4620]
    #27096770 - 12/18/20 10:12 PM (3 years, 1 month ago)

haha no doubt I never did shit anyway so not much change for me:lol:


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OfflineEugene Gesuale
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Re: How many agar transfers prior to grain spawn? [Re: cronicr]
    #27096857 - 12/18/20 11:07 PM (3 years, 1 month ago)

JCM, can you share with me the way you did your solo cup grows? Did you case or sub ratio? Did you set another solo inside it to help surface maintain rh? Any holes for fae?


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Re: How many agar transfers prior to grain spawn? [Re: Eugene Gesuale]
    #27096924 - 12/19/20 12:25 AM (3 years, 1 month ago)

Quote:

Adrenochrome said:
JCM, can you share with me the way you did your solo cup grows? Did you case or sub ratio? Did you set another solo inside it to help surface maintain rh? Any holes for fae?




Would like to know as well :stoned:


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Offlineel gordo
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Re: How many agar transfers prior to grain spawn? [Re: Shroomysamba]
    #27097024 - 12/19/20 03:24 AM (3 years, 1 month ago)

somebody knows why u can do t5, t6, t7, t8 plates with no problems and in grain in 3 transfers the mycelium is suposedly tired????? have no sense to me that is related with aging instead of accumulation/lack of something caused by the nutrient itself...


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Offlinejcm4620
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Re: How many agar transfers prior to grain spawn? [Re: el gordo]
    #27097049 - 12/19/20 04:20 AM (3 years, 1 month ago)

i just made up my normal grain and sub ratio of 1.4 the last tjme i did them but now im at 1.5 but either or is fine. ul need to figure out how much total it will take to fill how many of them to a 1.875(1-7/8ths) depth cuz i cant remember at the moment tho. then id use baby food sized or even a lil bigger jars to use for the grain and then just pc as normal. then as far as the sub goes once i had the amount figured out i just make it as usual at one time. then each grain jar is knocked up via a small agar wedge and the rest is normal. same casing at same .125(1/8th inch) depth. the cups are just cut down to where they are about the 2.0 inch depth or just a cunt hair over. then i would spawn them to the cups and just layer sub grain sub grain ect. ect. ect. also dont forget to label correctly. each jar each cup and a ziplock sandwich bag for each one. after each one is spawned they are placed inside the bag that has a few needle holes in it for gas exchange. allow to colonize as normal but they will be fast as hell. then id case and place them into my fcs as i do my trays.

thats basicly it in a nutshell 🀷🏼


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Edited by jcm4620 (12/19/20 04:24 AM)


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OfflineEugene Gesuale
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Re: How many agar transfers prior to grain spawn? [Re: jcm4620]
    #27097072 - 12/19/20 05:02 AM (3 years, 1 month ago)

Thanks man!:prettyflyforawhiteguy:


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