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OfflinePeterPumpkinEater
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why flame sterilize if you can buy disposable sterile needles?
    #27091459 - 12/16/20 12:50 AM (3 years, 2 months ago)

syringe needles cost so little, why flame sterilize if instead you could buy like 100 needles in their little sterile container and unwrap each for every scrape or transfer as an innocuation lope. Flame sterilization is totally passe in my opinion. (awaiting flames)


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OnlineLogicaL ChaosM
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Re: why flame sterilize if you can buy disposable sterile needles? [Re: PeterPumpkinEater] * 1
    #27091460 - 12/16/20 12:52 AM (3 years, 2 months ago)

Well, thats really wasteful, even if they dont cost very much. If its possible to reduce waste in Cultivation, it should be used.


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Offlinepureshrooming
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Re: why flame sterilize if you can buy disposable sterile needles? [Re: LogicaL Chaos] * 1
    #27091464 - 12/16/20 12:59 AM (3 years, 2 months ago)

Not only wasteful but tedious. Unwrap a new needle every single time you do anything or reach your arm a foot away and hold whatever you're using to it for 10s or less?


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Re: why flame sterilize if you can buy disposable sterile needles? [Re: PeterPumpkinEater] * 1
    #27091477 - 12/16/20 01:16 AM (3 years, 2 months ago)

Flame sterilization is an effective method, tried and true and used millions of times with great success.

That being said, I have done literally thousands of incouculations without flame sterilization, but I use some decent sterile protocols.

Flame sterilization is an easy way to make sure you know there is no contamination in the needle. Is it mandatory for success? No. Can it help your chances? Yes.


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OfflineFRUITS
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Re: why flame sterilize if you can buy disposable sterile needles? [Re: george castanza] * 1
    #27091482 - 12/16/20 01:23 AM (3 years, 2 months ago)

I flame for the total peace of mind, plus I typically use a needle a few times before it gets kind of dull. Just trying to reduce my plastic waste as much as possible.


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Offlinefltdriver82
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Re: why flame sterilize if you can buy disposable sterile needles? [Re: FRUITS]
    #27091486 - 12/16/20 01:33 AM (3 years, 2 months ago)

I flamed my first ever batch (circa 2007ish) but since then I just pc the needle, I will wipe with alcohol in between nocs if I have to set syringe down or touch something not sterile...

To me the idea of an open flame around things I have just wiped with alcohol isn't ideal, not to mention that the flame creates an updraft and my main clean goal is a still air room.


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OfflineCatsLoveHouseMusic
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Re: why flame sterilize if you can buy disposable sterile needles? [Re: fltdriver82]
    #27091494 - 12/16/20 01:50 AM (3 years, 2 months ago)

I started growing the beginning of this year so have a handle on things but still trying to learn, does it HAVE to get red hot? Is the light orange glow not enough?


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OfflinePeterPumpkinEater
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Re: why flame sterilize if you can buy disposable sterile needles? [Re: pureshrooming]
    #27091498 - 12/16/20 02:11 AM (3 years, 2 months ago)

Quote:

pureshrooming said:
Not only wasteful but tedious. Unwrap a new needle every single time you do anything or reach your arm a foot away and hold whatever you're using to it for 10s or less?




Unwrap? Surely you jest, sir. For the wrapper is a pithy to remove.

See that's just it, CastLoveHouseMusic. You wouldn't need to flame anything, if you just used needles... I say removing the wrapper in a snap is easier than the dexterity required for flame sterilization, anyways. Just saying...

I will not be flame sterilizing at all. Long live disposable presterilized needles come in wrappers.

And no the needles can be right there in your glove box / SAB right next to your ... two hands are enough... right next to your petri dishes...
why would you have to reach for them?

Anyways, flame sterilization and unwrapping both require some dexterity.

My point is just flame sterilization isn't necessary if the needles are de facto and de jure, sold as sterile equipment - or they wouldn't be used in hospitals...

my peace of mind is in knowing this, rather than having to strike a lighter every time I wan't to do a transfer. Another plastic wrapper, another day.

Its not like you take your own wicker basket to McDonalds or Tesco's or Walmart coz you wanna save the earth... even Norwegian scientists say its not possible, there's already too much shit - But I think of course the Moon and Mars, etc. will be very clean spaces...


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Edited by PeterPumpkinEater (12/16/20 02:19 AM)


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InvisiblebodhisattaMDiscordReddit
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Re: why flame sterilize if you can buy disposable sterile needles? [Re: PeterPumpkinEater]
    #27091571 - 12/16/20 04:58 AM (3 years, 2 months ago)

Even if you unwrap a new clean needle you should flame it. Your gloved hands can introduce contaminants when you open the package or take the cap off the needle if it has one. We're injecting sterile media not mammals with immune systems.

Also you're not doing it right. You don't strike a lighter every time you need to flame. You should not be using a lighter to flame anyway. Use a torch and leave it on during your work.


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Invisibleseldom seen
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Re: why flame sterilize if you can buy disposable sterile needles? [Re: bodhisatta]
    #27091642 - 12/16/20 06:51 AM (3 years, 2 months ago)

I'm too ocd not to flame even if it's already 'sterile'.


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Offlinepureshrooming
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Re: why flame sterilize if you can buy disposable sterile needles? [Re: PeterPumpkinEater]
    #27092106 - 12/16/20 12:34 PM (3 years, 2 months ago)

Quote:

PeterPumpkinEater said:

Unwrap? Surely you jest, sir. For the wrapper is a pithy to remove.

See that's just it, CastLoveHouseMusic. You wouldn't need to flame anything, if you just used needles... I say removing the wrapper in a snap is easier than the dexterity required for flame sterilization, anyways. Just saying...

I will not be flame sterilizing at all. Long live disposable presterilized needles come in wrappers.

And no the needles can be right there in your glove box / SAB right next to your ... two hands are enough... right next to your petri dishes...
why would you have to reach for them?

Anyways, flame sterilization and unwrapping both require some dexterity.

My point is just flame sterilization isn't necessary if the needles are de facto and de jure, sold as sterile equipment - or they wouldn't be used in hospitals...

my peace of mind is in knowing this, rather than having to strike a lighter every time I wan't to do a transfer. Another plastic wrapper, another day.

Its not like you take your own wicker basket to McDonalds or Tesco's or Walmart coz you wanna save the earth... even Norwegian scientists say its not possible, there's already too much shit - But I think of course the Moon and Mars, etc. will be very clean spaces...





Dexterity required for flame sterilization?! Dude you reach over to your burner and hold the scalpel to a flame :crazy:

If you are using a lighter then I can see why you would post this. Get a burner and your world will change. If you are relying solely on needles you are also missing out on a full range of tools; scalpel, tweezers, scissors, and of course any needles you need. Bonus - if someone happens to see in your trashcan they aren't going to think you are shooting up.


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OfflineOvoidhunter
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Re: why flame sterilize if you can buy disposable sterile needles? [Re: pureshrooming] * 1
    #27092112 - 12/16/20 12:38 PM (3 years, 2 months ago)

I've been using the same scapel blade the last six months. :shrug:


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OfflinePeterPumpkinEater
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Re: why flame sterilize if you can buy disposable sterile needles? [Re: pureshrooming]
    #27093747 - 12/17/20 09:12 AM (3 years, 1 month ago)

Quote:

pureshrooming said:
Quote:

PeterPumpkinEater said:

Unwrap? Surely you jest, sir. For the wrapper is a pithy to remove.

See that's just it, CastLoveHouseMusic. You wouldn't need to flame anything, if you just used needles... I say removing the wrapper in a snap is easier than the dexterity required for flame sterilization, anyways. Just saying...

I will not be flame sterilizing at all. Long live disposable presterilized needles come in wrappers.

And no the needles can be right there in your glove box / SAB right next to your ... two hands are enough... right next to your petri dishes...
why would you have to reach for them?

Anyways, flame sterilization and unwrapping both require some dexterity.

My point is just flame sterilization isn't necessary if the needles are de facto and de jure, sold as sterile equipment - or they wouldn't be used in hospitals...

my peace of mind is in knowing this, rather than having to strike a lighter every time I wan't to do a transfer. Another plastic wrapper, another day.

Its not like you take your own wicker basket to McDonalds or Tesco's or Walmart coz you wanna save the earth... even Norwegian scientists say its not possible, there's already too much shit - But I think of course the Moon and Mars, etc. will be very clean spaces...





Dexterity required for flame sterilization?! Dude you reach over to your burner and hold the scalpel to a flame :crazy:

If you are using a lighter then I can see why you would post this. Get a burner and your world will change. If you are relying solely on needles you are also missing out on a full range of tools; scalpel, tweezers, scissors, and of course any needles you need. Bonus - if someone happens to see in your trashcan they aren't going to think you are shooting up.




I use a concoction of methylated spirits and filtered cinnamon to quite literally sterilize my SAB. I have a link on a research article on the totally deadly efficacy on fungi of cinnamon extracted with methanol. I don't want to risk striking anything in there. That is just for resterilizing but in general I just wipe down with bleach water, and also my cloth at the bottom is also moist with bleach water. But, as a precautionary measure, I use a deadly concoction of methanol-extracted cinnamon. I have the article here somewhere, fungi and bacteria can't stand the stuff... no flame for me. However I am getting slightly worried as the smell is highly annoying as I know it can melt your eyeballs or smth...


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Offlinepureshrooming
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Re: why flame sterilize if you can buy disposable sterile needles? [Re: PeterPumpkinEater]
    #27093864 - 12/17/20 10:40 AM (3 years, 1 month ago)

Hows that process working out for you so far?


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OfflineCatsLoveHouseMusic
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Re: why flame sterilize if you can buy disposable sterile needles? [Re: PeterPumpkinEater]
    #27093956 - 12/17/20 11:27 AM (3 years, 1 month ago)

I’ve been using diluted kitten urine magnetized with copper, there some sort of symbiotic relationship that causes bacteria to flee. It’s a delicate procedure but if you follow the tek perfectly it will work.


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OfflinePeterPumpkinEater
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Re: why flame sterilize if you can buy disposable sterile needles? [Re: pureshrooming]
    #27094077 - 12/17/20 12:36 PM (3 years, 1 month ago)

Quote:

pureshrooming said:
Hows that process working out for you so far?




By the tone of mood in your comment it would suggest you already know...
why would using single-use pre-sterilized needles be in any way suggestive of being problematic compared to a little flame thrower?

And as for second professor's little joke, here is the article about cinnamon extracted using methanol...

Kitten Urine magnetised with copper you say?
Perhaps you can link me to the research article that was peer reviewed like this one regarding a comparison between methanol, ethanol, and water extracts of cinnammomum zeylanicum?

https://drive.google.com/file/d/1A-klf3S9sanF0NcaOF5m-yHIe-Hh4fK3/view?usp=sharing

I hope you take the time to actually read the article. I can assure you my concoction does not let things live... :smile:

And, I have a slew of other articles relating to the antifungal properties of herbs and spices, if you filter through some of my other posts, you shall find the links, I shan't bother you with any of this information, obviously its just "hogwash" and not a nasty deadly antifungal concoction to you or the organisms who attempt to find the potential for life in my SAB :tongue:


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Edited by PeterPumpkinEater (12/17/20 12:38 PM)


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Re: why flame sterilize if you can buy disposable sterile needles? [Re: bodhisatta]
    #27094086 - 12/17/20 12:43 PM (3 years, 1 month ago)

Quote:

bodhisatta said:
Even if you unwrap a new clean needle you should flame it. Your gloved hands can introduce contaminants when you open the package or take the cap off the needle if it has one. We're injecting sterile media not mammals with immune systems.

Also you're not doing it right. You don't strike a lighter every time you need to flame. You should not be using a lighter to flame anyway. Use a torch and leave it on during your work.




:whathesaid:

Anytime you open anything you are creating a particle dispersion, like a sneeze but not as severe. Particles are generated by everything that you do, this is why you need to be cognizant of every movement or your sterile procedure will be dog shit even though you're convinced that you're gtg. It will create an "It is impossible for a man to learn what he thinks he already knows" type scenario.

This will lead to many threads asking what's up with your grow.


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Re: why flame sterilize if you can buy disposable sterile needles? [Re: Stipe-n Cap]
    #27094111 - 12/17/20 12:55 PM (3 years, 1 month ago)

Why would you use some unnecessary chemical that has an annoying smell and can "melt your eyeballs" rather than doing the validated SAB procedure. If it's not any easier than the proven method then it's not worth deviating.


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Re: why flame sterilize if you can buy disposable sterile needles? [Re: Lemgrub] * 1
    #27094131 - 12/17/20 01:03 PM (3 years, 1 month ago)



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Offlinepureshrooming
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Re: why flame sterilize if you can buy disposable sterile needles? [Re: PeterPumpkinEater]
    #27094251 - 12/17/20 02:04 PM (3 years, 1 month ago)

Quote:

PeterPumpkinEater said:
By the tone of mood in your comment it would suggest you already know...
why would using single-use pre-sterilized needles be in any way suggestive of being problematic compared to a little flame thrower?

And as for second professor's little joke, here is the article about cinnamon extracted using methanol...

Kitten Urine magnetised with copper you say?
Perhaps you can link me to the research article that was peer reviewed like this one regarding a comparison between methanol, ethanol, and water extracts of cinnammomum zeylanicum?

https://drive.google.com/file/d/1A-klf3S9sanF0NcaOF5m-yHIe-Hh4fK3/view?usp=sharing

I hope you take the time to actually read the article. I can assure you my concoction does not let things live... :smile:

And, I have a slew of other articles relating to the antifungal properties of herbs and spices, if you filter through some of my other posts, you shall find the links, I shan't bother you with any of this information, obviously its just "hogwash" and not a nasty deadly antifungal concoction to you or the organisms who attempt to find the potential for life in my SAB :tongue:




I feel like you are trying to reinvent the wheel but you don't even know what a circle is yet. Not trying to be a smartass or anything (well maybe a little).

I'm all about innovating and trying new things but that's best served by people who have a solid, practicing understanding of the basics and why they are done that way. Experience is what allows you to make those decisions on how you can deviate. That is why I ask how this process is working out for you. If you posted pictures of plates you made using your process and they were perfectly clean, that would certainly change reactions.

I never even said the needle wouldn't work, just several reasons why it wasn't better. I still would flame sterilize a fresh needle before touching it to a sterile plate tho.


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