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Invisiblesavan73
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THE BEST OPTION is LC to SPAWN BAG *DELETED*
    #27091589 - 12/16/20 05:28 AM (3 years, 1 month ago)

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Offlinejomanda1990
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Re: THE BEST OPTION is LC to SPAWN BAG [Re: savan73]
    #27091618 - 12/16/20 06:16 AM (3 years, 1 month ago)

How are you going to make clean LC without agar? It's not optional; if you want LC, you need the absolute cleanest culture to make it.


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Offlinebw86
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Re: THE BEST OPTION is LC to SPAWN BAG [Re: jomanda1990]
    #27091621 - 12/16/20 06:18 AM (3 years, 1 month ago)

IMO that's the worst option.
To each their own. "everybody fucks funny to somebody"

Agar to grain. grain to grain. --- unlimited mushrooms.


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Invisiblesavan73
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Re: THE BEST OPTION is LC to SPAWN BAG *DELETED* [Re: jomanda1990]
    #27091627 - 12/16/20 06:31 AM (3 years, 1 month ago)

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OfflineFunnyFungiName
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Re: THE BEST OPTION is LC to SPAWN BAG [Re: savan73]
    #27091782 - 12/16/20 08:50 AM (3 years, 1 month ago)

Start with a clean agar culture and try one of these.

https://www.shroomery.org/13884/Liquid-Inoculant-LI


Edited by FunnyFungiName (12/16/20 08:51 AM)


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Offlinejomanda1990
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Re: THE BEST OPTION is LC to SPAWN BAG [Re: savan73] * 2
    #27091922 - 12/16/20 10:32 AM (3 years, 1 month ago)

Quote:

savan73 said:
Quote:

jomanda1990 said:
How are you going to make clean LC without agar?




With spore syringe. I did spore syringe to grain once and it worked well. I mean why not? It could be work on liquid, also less contam risk.




Spore syringes are much more likely to have contaminants than any other inoculation technique you could use.
They are made from spores obtained from mushrooms, which grow in non-sterile conditions (they need fresh air, which is teeming with bacteria and mold spores).
You can try but nutritious LC is the IDEAL meduim for bacteria to grow and ruin your project whitout you even noticing before you spawn to bulk.
Please consider going through the stickied guides which explain why syringe to grain (or LC for that matter) is a bad idea.

Agar is easy AF and relatively cheap if you can get any sort of plastic PP5 container that can be pressure cooked (so that you don't have to pour agar if you don't like to or can't)


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Edited by jomanda1990 (12/16/20 10:34 AM)


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OfflineLtLurker
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Re: THE BEST OPTION is LC to SPAWN BAG [Re: jomanda1990]
    #27092045 - 12/16/20 11:52 AM (3 years, 1 month ago)

:whathesaid:

To over simplify, you have 2 starting routes. Either shoot spores into brf cakes; or put spores to agar, clean up, then go to grains, lc, li, etc.

Whether you like it or not, SAB for sterile work is a requirement.


Edited by LtLurker (12/16/20 11:55 AM)


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