Home | Community | Message Board

Original Seeds Store
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Unfolding Nature Unfolding Nature: Being in the Implicate Order   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Liquid Cultures   Original Sensible Seeds Bulk Cannabis Seeds   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Bridgetown Botanicals CBD Concentrates   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies

Jump to first unread post Pages: 1
Offlinealex66
Stranger
Registered: 11/14/20
Posts: 6
Last seen: 3 years, 1 month
Questions about weird mycelium on agar growth (with photos)
    #27090801 - 12/15/20 04:09 PM (3 years, 1 month ago)

Hello,
I have been growing some mycelium on some agar plates, I'd like to hear your opinion about some "strange" mycelium formations, to figure out if there are contaminations or something to worry about.

 

Photo 1 and 2: in light blue you can see what I believe is the mycelium. Circled in orange there is something I can't figure out if it is just some more mycelium or some kind of mold. I would say it is mycelium, but to me it appears more "fluffy" than the rest, and somewhat more regular in its shape.



Photo 3: Circled in blue you can see some "protuberances" of the mycelium, is it normal to have them? 

 

Photo 4 and 5: I noticed a faint yellow ring on the mycelium on most my plates. I tried to take a photo of it, but it didn't show up. I fiddled with the setting of the camera and i managed to have the ring show up with a realistic color. The problem is that other parts of the photo are underexposed or have got weird colors. Circled in green you can see the yellow ring I am trying to identify. Circled in red there is a spot that appears grey/black, in reality it is just as white as the other part of the mycelium, but a drop of water and a small depression on the agar creates that effect. Please don't consider it. In fuchsia you can see a small spot of bacteria.

All the work with the plates have been done in a SAB.

Thank you


Extras: Filter Print Post Top
Invisiblesandman420
Saint PP
Male


Registered: 06/17/04
Posts: 5,384
Trusted Cultivator
Re: Questions about weird mycelium on agar growth (with photos) [Re: alex66]
    #27090831 - 12/15/20 04:28 PM (3 years, 1 month ago)

none of that is mycelium from a cubensis mushrooms.

Unless that is some edible or exotic it is not what you are looking for and there is 0 healthy mycelium to transfer and you should throw these away. And probably the spore source they came from most likely too.

It's white mold


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed


Extras: Filter Print Post Top
InvisibleMelliferous
🌵🍄🌵🍄🌵
 Unread Journal


Registered: 10/01/20
Posts: 1,053
Re: Questions about weird mycelium on agar growth (with photos) [Re: sandman420]
    #27090834 - 12/15/20 04:29 PM (3 years, 1 month ago)

Quote:

sandman420 said:
none of that is mycelium from a cubensis mushrooms.

Unless that is some edible or exotic it is not what you are looking for and there is 0 healthy mycelium to transfer and you should throw these away. And probably the spore source they came from most likely too.

It's white mold



:whathesaid:


--------------------


"No, I don't worry. I tell you, I am a man who believed that I died 20 years ago, and I live like a man who is dead already. I have no fear, whatsoever, of anybody or anything."


Extras: Filter Print Post Top
OfflineAbabyphoenix
You
I'm a teapot User Gallery


Registered: 03/21/19
Posts: 1,015
Loc: Human Body
Last seen: 24 days, 23 hours
Re: Questions about weird mycelium on agar growth (with photos) [Re: Melliferous]
    #27090839 - 12/15/20 04:33 PM (3 years, 1 month ago)

:whathesaid:

no good. sorry main


--------------------


:sporedrop::aum:Pho's MushiAventures::aum::sporedrop:
:heartpump:My '21 GLOG:heartpump:
:peace: LAGM 2022 :peace:



Extras: Filter Print Post Top
Offlinealex66
Stranger
Registered: 11/14/20
Posts: 6
Last seen: 3 years, 1 month
Re: Questions about weird mycelium on agar growth (with photos) [Re: alex66]
    #27090878 - 12/15/20 04:57 PM (3 years, 1 month ago)

Cool. I have been happily growing mold for 20 days then. It was supposed to be Cubensis hawaiiana.

How have you identified that it is white mold? So i don't repeat the mistake in future. I have browsed various threads about contamination and how it should look like, but mold 1, me 0, for now i guess.


Extras: Filter Print Post Top
Offlineel gordo
not the youtube one
Male User Gallery


Registered: 09/05/20
Posts: 108
Last seen: 1 month, 19 days
Re: Questions about weird mycelium on agar growth (with photos) [Re: alex66]
    #27090895 - 12/15/20 05:07 PM (3 years, 1 month ago)

it look like cubensis mycelium to me on the last pictures,  on the edges its growing inside the agar thats why it looks so whispy, this yellowing and strange growth could be because of the bacteria  and water pooling that can be seen, what agar reciepe u used? looks strange on some pics


Edited by el gordo (12/15/20 05:13 PM)


Extras: Filter Print Post Top
InvisibleLemgrub
 User Gallery


Registered: 04/02/18
Posts: 589
Re: Questions about weird mycelium on agar growth (with photos) [Re: el gordo]
    #27091131 - 12/15/20 08:23 PM (3 years, 1 month ago)

If the leading edge is more transparent than the rest of the colony that's a tell to me often. This mold mycelia is also not as 'organized', cubes grow in straighter, defined lines and is more uniform to my eye.


--------------------
We getting that pirate bay, alien shishkebab cordycep money


Extras: Filter Print Post Top
Offlinealex66
Stranger
Registered: 11/14/20
Posts: 6
Last seen: 3 years, 1 month
Re: Questions about weird mycelium on agar growth (with photos) [Re: el gordo]
    #27091527 - 12/16/20 03:30 AM (3 years, 1 month ago)

Quote:

el gordo said:
it look like cubensis mycelium to me on the last pictures,  on the edges its growing inside the agar thats why it looks so whispy, this yellowing and strange growth could be because of the bacteria  and water pooling that can be seen, what agar reciepe u used? looks strange on some pics




The agar recipe is


400 ml water
7.8 g of agar
10 g malt extract (the creamy brown substance)
10 g corn flour
6 drops of blue food colorant


I have got more plates, i will post a photos of them, because the growth looks diffent than the ones i have posted already. Maybe we can identify it better


Extras: Filter Print Post Top
Offlineel gordo
not the youtube one
Male User Gallery


Registered: 09/05/20
Posts: 108
Last seen: 1 month, 19 days
Re: Questions about weird mycelium on agar growth (with photos) [Re: alex66]
    #27091542 - 12/16/20 04:18 AM (3 years, 1 month ago)

i think the reciepe has too much nutrients, maybe thats why its acting weird, try to transfer pieces as far as posible from the bacterial/slimy spots to

400 ml water
8 grams light malt extract
8 grams agar

it should grow better there with this simpler reciepe


Edited by el gordo (12/16/20 04:21 AM)


Extras: Filter Print Post Top
Offlinealex66
Stranger
Registered: 11/14/20
Posts: 6
Last seen: 3 years, 1 month
Re: Questions about weird mycelium on agar growth (with photos) [Re: el gordo]
    #27092062 - 12/16/20 12:03 PM (3 years, 1 month ago)

Quote:

el gordo said:
i think the reciepe has too much nutrients, maybe thats why its acting weird, try to transfer pieces as far as posible from the bacterial/slimy spots to

400 ml water
8 grams light malt extract
8 grams agar

it should grow better there with this simpler reciepe




I will try with this recipe next time, thank you.

Those are the photos of the other two plates, that looks somewhat different. Is it cubensis or some more mold that abused of my grower love? :grin:

   


Extras: Filter Print Post Top
Invisiblesandman420
Saint PP
Male


Registered: 06/17/04
Posts: 5,384
Trusted Cultivator
Re: Questions about weird mycelium on agar growth (with photos) [Re: alex66]
    #27092069 - 12/16/20 12:10 PM (3 years, 1 month ago)

The 10g of corn flour on top of the extra malty MEA recipe could possibly explain why it looks so crazy.

Normal is 500g water, 10g agar, 10g malt

and you did less water and less agar (no prob on the agar %) PLUS additional flour stuff which was unnecesary.

What is the inoculum, a tissue clone?

Id transfer to a good recipe like gordo gave you and then see if it hopefully looks better.

good luck


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed


Edited by sandman420 (12/16/20 12:21 PM)


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Unfolding Nature Unfolding Nature: Being in the Implicate Order   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Liquid Cultures   Original Sensible Seeds Bulk Cannabis Seeds   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Bridgetown Botanicals CBD Concentrates   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies


Similar ThreadsPosterViewsRepliesLast post
* WBS jar stalling after agar inoculation (photo) Josborne 866 14 05/04/21 02:29 PM
by Stipe-n Cap
* question about mycelium..... sancho 863 13 12/08/02 06:06 PM
by MuShARoom
* Agar growth ID
( 1 2 all )
drbobgoatse 615 22 05/06/21 03:26 PM
by drbobgoatse
* weird mycelium ? *DELETED* nishikkiknee 659 2 06/18/01 08:07 PM
by Frothy
* question about mycelium growth staindtrip 1,274 4 01/01/06 02:13 PM
by shroomer68
* how long for agar growth? Anonymous 2,224 2 12/07/02 09:53 AM
by rhizo
* Re: Multispore Mycelium Agar Culturing TEK sutec 2,430 1 12/03/99 10:55 AM
by Anonymous
* Questions about using Agar! shroomeritus 863 2 05/22/01 11:56 PM
by sylo

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,400 topic views. 19 members, 172 guests and 50 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.022 seconds spending 0.007 seconds on 12 queries.