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alex66
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Questions about weird mycelium on agar growth (with photos)
#27090801 - 12/15/20 04:09 PM (3 years, 1 month ago) |
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Hello, I have been growing some mycelium on some agar plates, I'd like to hear your opinion about some "strange" mycelium formations, to figure out if there are contaminations or something to worry about.

Photo 1 and 2: in light blue you can see what I believe is the mycelium. Circled in orange there is something I can't figure out if it is just some more mycelium or some kind of mold. I would say it is mycelium, but to me it appears more "fluffy" than the rest, and somewhat more regular in its shape.

Photo 3: Circled in blue you can see some "protuberances" of the mycelium, is it normal to have them?

Photo 4 and 5: I noticed a faint yellow ring on the mycelium on most my plates. I tried to take a photo of it, but it didn't show up. I fiddled with the setting of the camera and i managed to have the ring show up with a realistic color. The problem is that other parts of the photo are underexposed or have got weird colors. Circled in green you can see the yellow ring I am trying to identify. Circled in red there is a spot that appears grey/black, in reality it is just as white as the other part of the mycelium, but a drop of water and a small depression on the agar creates that effect. Please don't consider it. In fuchsia you can see a small spot of bacteria.
All the work with the plates have been done in a SAB.
Thank you
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sandman420
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Re: Questions about weird mycelium on agar growth (with photos) [Re: alex66]
#27090831 - 12/15/20 04:28 PM (3 years, 1 month ago) |
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none of that is mycelium from a cubensis mushrooms.
Unless that is some edible or exotic it is not what you are looking for and there is 0 healthy mycelium to transfer and you should throw these away. And probably the spore source they came from most likely too.
It's white mold
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Melliferous
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Re: Questions about weird mycelium on agar growth (with photos) [Re: sandman420]
#27090834 - 12/15/20 04:29 PM (3 years, 1 month ago) |
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Quote:
sandman420 said: none of that is mycelium from a cubensis mushrooms.
Unless that is some edible or exotic it is not what you are looking for and there is 0 healthy mycelium to transfer and you should throw these away. And probably the spore source they came from most likely too.
It's white mold
--------------------
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Ababyphoenix
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Re: Questions about weird mycelium on agar growth (with photos) [Re: Melliferous]
#27090839 - 12/15/20 04:33 PM (3 years, 1 month ago) |
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no good. sorry main
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alex66
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Re: Questions about weird mycelium on agar growth (with photos) [Re: alex66]
#27090878 - 12/15/20 04:57 PM (3 years, 1 month ago) |
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Cool. I have been happily growing mold for 20 days then. It was supposed to be Cubensis hawaiiana.
How have you identified that it is white mold? So i don't repeat the mistake in future. I have browsed various threads about contamination and how it should look like, but mold 1, me 0, for now i guess.
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el gordo
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Re: Questions about weird mycelium on agar growth (with photos) [Re: alex66]
#27090895 - 12/15/20 05:07 PM (3 years, 1 month ago) |
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it look like cubensis mycelium to me on the last pictures, on the edges its growing inside the agar thats why it looks so whispy, this yellowing and strange growth could be because of the bacteria and water pooling that can be seen, what agar reciepe u used? looks strange on some pics
Edited by el gordo (12/15/20 05:13 PM)
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Lemgrub



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Re: Questions about weird mycelium on agar growth (with photos) [Re: el gordo]
#27091131 - 12/15/20 08:23 PM (3 years, 1 month ago) |
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If the leading edge is more transparent than the rest of the colony that's a tell to me often. This mold mycelia is also not as 'organized', cubes grow in straighter, defined lines and is more uniform to my eye.
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alex66
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Re: Questions about weird mycelium on agar growth (with photos) [Re: el gordo]
#27091527 - 12/16/20 03:30 AM (3 years, 1 month ago) |
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Quote:
el gordo said: it look like cubensis mycelium to me on the last pictures, on the edges its growing inside the agar thats why it looks so whispy, this yellowing and strange growth could be because of the bacteria and water pooling that can be seen, what agar reciepe u used? looks strange on some pics
The agar recipe is
400 ml water 7.8 g of agar 10 g malt extract (the creamy brown substance) 10 g corn flour 6 drops of blue food colorant
I have got more plates, i will post a photos of them, because the growth looks diffent than the ones i have posted already. Maybe we can identify it better
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el gordo
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Re: Questions about weird mycelium on agar growth (with photos) [Re: alex66]
#27091542 - 12/16/20 04:18 AM (3 years, 1 month ago) |
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i think the reciepe has too much nutrients, maybe thats why its acting weird, try to transfer pieces as far as posible from the bacterial/slimy spots to
400 ml water 8 grams light malt extract 8 grams agar
it should grow better there with this simpler reciepe
Edited by el gordo (12/16/20 04:21 AM)
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alex66
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Re: Questions about weird mycelium on agar growth (with photos) [Re: el gordo]
#27092062 - 12/16/20 12:03 PM (3 years, 1 month ago) |
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Quote:
el gordo said: i think the reciepe has too much nutrients, maybe thats why its acting weird, try to transfer pieces as far as posible from the bacterial/slimy spots to
400 ml water 8 grams light malt extract 8 grams agar
it should grow better there with this simpler reciepe
I will try with this recipe next time, thank you.
Those are the photos of the other two plates, that looks somewhat different. Is it cubensis or some more mold that abused of my grower love? 
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sandman420
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Re: Questions about weird mycelium on agar growth (with photos) [Re: alex66]
#27092069 - 12/16/20 12:10 PM (3 years, 1 month ago) |
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The 10g of corn flour on top of the extra malty MEA recipe could possibly explain why it looks so crazy.
Normal is 500g water, 10g agar, 10g malt
and you did less water and less agar (no prob on the agar %) PLUS additional flour stuff which was unnecesary.
What is the inoculum, a tissue clone?
Id transfer to a good recipe like gordo gave you and then see if it hopefully looks better.
good luck
Edited by sandman420 (12/16/20 12:21 PM)
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