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OfflineMarbella
Registered: 12/16/19
Posts: 8
Last seen: 2 years, 11 months
How to leverage my Mono-culture
    #27081211 - 12/09/20 08:58 PM (3 years, 2 months ago)

Once I isolate and grow a mono-culture, how can I replicate and leverage all the hard work?

Can I

1. IF i transfer a tissue to a grain jar, will the mycelium in the jar be mono? I assume yes.
2. Can I then perform grain to grain transfers and replicate the mono-culture?
3. Can I transfer a tissue sample to a liquid inoculant and grow mono-liquid-culture?
4. is this when I store a sample on a slant to be the master?


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OfflineLeon ForestFacebookDiscord
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Registered: 02/11/13
Posts: 574
Last seen: 1 month, 20 days
Re: How to leverage my Mono-culture [Re: Marbella]
    #27090463 - 12/15/20 12:43 PM (3 years, 2 months ago)

Quote:

Marbella said:
Once I isolate and grow a mono-culture, how can I replicate and leverage all the hard work?

Can I

1. IF i transfer a tissue to a grain jar, will the mycelium in the jar be mono? I assume yes.
2. Can I then perform grain to grain transfers and replicate the mono-culture?
3. Can I transfer a tissue sample to a liquid inoculant and grow mono-liquid-culture?
4. is this when I store a sample on a slant to be the master?




1. yes
2. yes
3. i would transfer to agar then use a clean piece of the culture on agar to inoculate lc or use the plate to make LI
4. You can slant anything you want, if you like what your getting, yes.


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InvisibleVinci
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Registered: 08/26/19
Posts: 629
Re: How to leverage my Mono-culture [Re: Leon Forest]
    #27090570 - 12/15/20 01:43 PM (3 years, 2 months ago)

Make LC. Mono will remain mono. LC can be expanded to insane amounts and stores very well. I used to mess with slants till I realized LC keeps for very long and is much easier to use as you don't have to grow out any petris, g2g jars or LCs when you want to use it again.


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