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OfflineTonyMushrana
is that a Trich again?
I'm a teapot User Gallery


Registered: 03/12/19
Posts: 323
Loc: Mountainly Place
Last seen: 1 year, 10 months
Re: Throw your jars away! [Re: fahtster]
    #27114249 - 12/29/20 01:19 PM (3 years, 30 days ago)

:takingnotes:


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Offlinedingdonger22
Stranger
Registered: 12/14/20
Posts: 35
Last seen: 2 years, 9 months
Re: Throw your jars away! [Re: TonyMushrana]
    #27137532 - 01/09/21 11:57 AM (3 years, 19 days ago)

Tried loading the AA sterilizer full up with bags and seem to be getting burst grains on one side of every bag. All the rest of the grain in the bag looks fine :confused:



Is it because they were touching the side of the sterilizer??? Everything I've read says thats not really an issue. I did these 9 bags (4lb oat in each) at 16-18 PSI for 3.5 hrs. Can these still be used or should I toss them and try again??


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OfflineSockadin
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Registered: 01/03/10
Posts: 7,244
Last seen: 2 months, 20 days
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Re: Throw your jars away! [Re: dingdonger22]
    #27137548 - 01/09/21 12:09 PM (3 years, 19 days ago)

They look like the grains are to wet. You can use them and it should recover as long as it remains clean. Burst grains just mean sticky mess, not contamination.


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Offlinedingdonger22
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Registered: 12/14/20
Posts: 35
Last seen: 2 years, 9 months
Re: Throw your jars away! [Re: Sockadin]
    #27137561 - 01/09/21 12:17 PM (3 years, 19 days ago)

Phew. I had thought the grain was headed for the trashcan. Why would they be burst only on one side though? Thats the confusing part for me. These things were dry to very dry when they went into the bags. Minus the grains on one side of each bag, they look fine.


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Invisiblemind.at.large
Myconerd


Registered: 12/13/16
Posts: 1,218
Loc: Floating in liquid gardens
Re: Throw your jars away! [Re: dingdonger22]
    #27137717 - 01/09/21 01:50 PM (3 years, 19 days ago)

If I have burst grains, it’s usually on the side that was touching the walls of the sterilizer, but some burst grains aren’t a problem


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Mind's Easy Bag 2 Bag Grain Transfers


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Invisiblemicelio
Song of Silence
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Registered: 04/22/14
Posts: 1,547
Re: Throw your jars away! [Re: TedsDead]
    #27147538 - 01/14/21 11:23 AM (3 years, 14 days ago)

Quote:

TedsDead said:
I agree with verum.  Ive had myc die in an lc when just stirred too much with stir bars




I tried fast speed on two jars last week for two hours. what a mistake.

The culture flatten out and laid dormant on the bottom of the jar.
They are just now coming back to life.

I have about 30 jars going now and I do allot of hand swirling.


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OfflineHaywire
Wetspot Wizard
Male


Registered: 12/29/13
Posts: 1,611
Last seen: 4 days, 18 hours
Re: Throw your jars away! [Re: mind.at.large]
    #27149033 - 01/15/21 03:35 AM (3 years, 13 days ago)

Quote:

mind.at.large said:
If I have burst grains, it’s usually on the side that was touching the walls of the sterilizer, but some burst grains aren’t a problem




I like to add some gypsum to the grains before sterilizing.
after the cycle, I give them a good shake in the jar and it tends to cover the open grains.


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Ciao mamma, guarda come mi diverto


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Offlinejunk_f00d
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Re: Throw your jars away! [Re: filthyknees]
    #27153657 - 01/17/21 02:42 PM (3 years, 11 days ago)

Quote:

filthyknees said:





How did you get it out so cleanly? What's your agar recipe?

*edit: oops nevermind, I see now you just cut a circle around it. at first glance I thought that empty plate next to the bag was all that was left after your transfer.  it looked like you got the whole thing in there, like you just turned your plae upside down and it fell right in. I've tried doing it like that before but haven't had luck.


Edited by junk_f00d (01/17/21 02:44 PM)


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Invisiblemind.at.large
Myconerd


Registered: 12/13/16
Posts: 1,218
Loc: Floating in liquid gardens
Re: Throw your jars away! [Re: Haywire]
    #27154021 - 01/17/21 06:39 PM (3 years, 11 days ago)

Quote:

Haywire said:
Quote:

mind.at.large said:
If I have burst grains, it’s usually on the side that was touching the walls of the sterilizer, but some burst grains aren’t a problem




I like to add some gypsum to the grains before sterilizing.
after the cycle, I give them a good shake in the jar and it tends to cover the open grains.



That's a good thought but to be honest, I've used gypsum for both grains and substrate and have never noticed any difference or benefit. Except gypsum is a pain in the ass to mix in a substrate cause it clumps like crazy


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Invisiblemind.at.large
Myconerd


Registered: 12/13/16
Posts: 1,218
Loc: Floating in liquid gardens
Re: Throw your jars away! [Re: junk_f00d]
    #27154024 - 01/17/21 06:41 PM (3 years, 11 days ago)

Quote:

junk_f00d said:
Quote:

filthyknees said:





How did you get it out so cleanly? What's your agar recipe?

*edit: oops nevermind, I see now you just cut a circle around it. at first glance I thought that empty plate next to the bag was all that was left after your transfer.  it looked like you got the whole thing in there, like you just turned your plae upside down and it fell right in. I've tried doing it like that before but haven't had luck.



I do mine with the whole agar puck. I do the typical MEA with 20 g/l of malt and agar. I can usually just pick the whole thing up with a scalpel and it comes out of the dish pretty easily


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Mind's Easy Bag 2 Bag Grain Transfers


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InvisibleTedsDead
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Registered: 01/03/17
Posts: 4,998
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Re: Throw your jars away! [Re: mind.at.large]
    #27154269 - 01/17/21 08:59 PM (3 years, 10 days ago)

If you over cook your grains gypsum can help kee0 them from clumping.  If mixing it in a substrate its best to mix it dry to avoid clumping.  No real benefit in a cube substrate tho.  In casings its used as a buffer


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weed gets you through times of no money better than money gets you through times of no weed...  -the fabulous furry freak bros
If you can buy it, you can burn it!



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OfflineListen
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Registered: 10/02/22
Posts: 14
Last seen: 1 year, 2 months
Re: Throw your jars away! [Re: mind.at.large]
    #28001751 - 10/16/22 12:39 PM (1 year, 3 months ago)

"thought about trying it with soft agar"

what about liquid culture?  Wouldn't that give you the quick colonization you want?

Or is the need to constantly test your LC  on agar too much of a bottle neck in the process ?


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Invisiblemind.at.large
Myconerd


Registered: 12/13/16
Posts: 1,218
Loc: Floating in liquid gardens
Re: Throw your jars away! [Re: Listen]
    #28002095 - 10/16/22 05:05 PM (1 year, 3 months ago)

Quote:

Listen said:
"thought about trying it with soft agar"

what about liquid culture?  Wouldn't that give you the quick colonization you want?

Or is the need to constantly test your LC  on agar too much of a bottle neck in the process ?



I mean yeah LC is great and would totally colonize quicker than this method. But there’s more than one way to skin a cat. This is just one of those ways.

I inoculate my bags many ways, LC, LI, master jars, wedges, bag to bag, etc. What I choose is usually dependent on what’s available. To be honest, I don’t really use this method that much anymore. If I wanted to use a plate to noc bags, I would probably blend it up first as LI. But either way, this method can and does work


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Mind's Easy Bag 2 Bag Grain Transfers


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Offlinesmalltalk_canceled
Babnik
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Registered: 07/13/20
Posts: 2,862
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Re: Throw your jars away! [Re: TedsDead]
    #28002149 - 10/16/22 05:51 PM (1 year, 3 months ago)

Quote:

TedsDead said:
If you over cook your grains gypsum can help kee0 them from clumping.  If mixing it in a substrate its best to mix it dry to avoid clumping.  No real benefit in a cube substrate tho.  In casings its used as a buffer




wtf


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Invisiblemind.at.large
Myconerd


Registered: 12/13/16
Posts: 1,218
Loc: Floating in liquid gardens
Re: Throw your jars away! [Re: smalltalk_canceled]
    #28002355 - 10/16/22 08:14 PM (1 year, 3 months ago)

Quote:

smalltalk_canceled said:
Quote:

TedsDead said:
If you over cook your grains gypsum can help kee0 them from clumping.  If mixing it in a substrate its best to mix it dry to avoid clumping.  No real benefit in a cube substrate tho.  In casings its used as a buffer




wtf



Huh?


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Mind's Easy Bag 2 Bag Grain Transfers


Endless Sub Tek


...the doll's trying to kill me and the toaster's been laughing at me...


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OfflineSyntex
Some Bloke
I'm a teapot


Registered: 10/02/22
Posts: 508
Loc: 🎑
Last seen: 4 months, 9 days
Re: Throw your jars away! [Re: mind.at.large]
    #28002373 - 10/16/22 08:33 PM (1 year, 3 months ago)

Quote:

mind.at.large said:
Quote:

smalltalk_canceled said:
Quote:

TedsDead said:
If you over cook your grains gypsum can help kee0 them from clumping.  If mixing it in a substrate its best to mix it dry to avoid clumping.  No real benefit in a cube substrate tho.  In casings its used as a buffer




wtf



Huh?



Wut?


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