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Offlineleuckart
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King Oyster, Shiitake and Enoki & Agar
    #27086753 - 12/13/20 05:45 AM (3 years, 2 months ago)

Dear Shroomery
Recently I tried cloning some grocery store mushroom mix onto brf agar plates (2% brf, 2% agar).
So far only shiitake did some decent job, producing nice mycelium at T+4 days after inoculation. Here's poor quality pic of my culture at day 6:

King oyster at T+2d grown a nice cob of mycelium around it but than it stopped. It ain't developing onto the agar at list visibly.
Is there a chance the agar is to hard? Would 1% agar do better?
My friend uses 1% or 0.5% agar in their microbiology lab, she was very surprised to hear we're doing 2% for cubes.

The culture of Enoki seems to be completely biologically inactive. No mycelium, no change, hell, even no contams, they look exactly like the day I put them. What could go wrong there?


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Offlinedeadmandave
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Re: King Oyster, Shiitake and Enoki & Agar [Re: leuckart]
    #27087101 - 12/13/20 10:41 AM (3 years, 2 months ago)

2% is correct. 20 grams per 1000g of water. take more attempts at cloning, usually 5-10 to insure a good clone.

btw those are the cutest agar jars i ever seen.


Edited by deadmandave (12/13/20 10:42 AM)


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Offlineleuckart
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Re: King Oyster, Shiitake and Enoki & Agar [Re: deadmandave]
    #27087668 - 12/13/20 05:49 PM (3 years, 2 months ago)

Thank you very much!
Update: seems that Enoki is growing something but very very slowly.


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