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OfflineEijin88
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Registered: 10/09/20
Posts: 70
Last seen: 2 years, 7 months
Contamination
    #27084992 - 12/12/20 03:11 AM (3 years, 1 month ago)

Hi everyone I think I have contamination here, I'm only looking for someone to confirm that for me please. Is there any chance I can get something from that?

https://ibb.co/s2K7PQW
https://ibb.co/YbJx8q4
https://ibb.co/tJFvTjq
https://ibb.co/Nx7KYyN
https://ibb.co/Gt9VPv3


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OfflineEijin88
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Registered: 10/09/20
Posts: 70
Last seen: 2 years, 7 months
Re: Contamination [Re: Eijin88]
    #27085129 - 12/12/20 06:50 AM (3 years, 1 month ago)

Please someone advi me what is happening with my substrate.


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OfflineCrackatoa
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I'm a teapot User Gallery


Registered: 03/31/19
Posts: 5,399
Loc: Over by your Mama's house Flag
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Trusted Cultivator
Re: Contamination [Re: Eijin88]
    #27085209 - 12/12/20 08:09 AM (3 years, 1 month ago)

It's not looking good buddy


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Crack's Tek's and Shit



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OfflineEijin88
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Registered: 10/09/20
Posts: 70
Last seen: 2 years, 7 months
Re: Contamination [Re: Crackatoa]
    #27085216 - 12/12/20 08:15 AM (3 years, 1 month ago)

Quote:

Crackatoa said:
It's not looking good buddy





Nothing like a constructive advice.


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OfflineCrackatoa
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I'm a teapot User Gallery


Registered: 03/31/19
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Re: Contamination [Re: Eijin88]
    #27085246 - 12/12/20 08:41 AM (3 years, 1 month ago)

I've never done any of those kits, I'm not sure what you can really do but throw away and try again. I would suggest getting a print and start reading up on agar. It's the only way of knowing what you got and what you are working with.


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Crack's Tek's and Shit



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OfflineEijin88
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Registered: 10/09/20
Posts: 70
Last seen: 2 years, 7 months
Re: Contamination [Re: Crackatoa]
    #27086557 - 12/13/20 12:30 AM (3 years, 1 month ago)

But what is happening? If you never did any of those how do you know that it doesn't look good and it's normal for these. Maybe just rye grain smashing against the walls. This is what I was hoping to learn here but people don't want to give any advice here...


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InvisibleLoadedFish
Psilocybin Enthusiast


Registered: 07/12/20
Posts: 107
Re: Contamination [Re: Eijin88]
    #27086591 - 12/13/20 01:37 AM (3 years, 1 month ago)

Looks bacterial as shit, and I’m confused as to why you’re complaining about not getting advice when the dude you’re replying to just gave you solid pointers.


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Offlinebunkster
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Registered: 11/21/20
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Last seen: 2 years, 10 months
Re: Contamination [Re: Eijin88]
    #27086616 - 12/13/20 02:17 AM (3 years, 1 month ago)

Quote:

Eijin88 said:
But what is happening? If you never did any of those how do you know that it doesn't look good and it's normal for these. Maybe just rye grain smashing against the walls. This is what I was hoping to learn here but people don't want to give any advice here...



Hey bud, I'm new here too. You've got contamination. That mycelium should be all white. The yellow/brownish stuff in-between is bacteria. It's most visible in your third and last photos.
Throw that tub away. Don't open it. Don't keep trying to grow it.
If you want advice from here, keep reading. Study some more tek and start over.


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OfflineEijin88
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Registered: 10/09/20
Posts: 70
Last seen: 2 years, 7 months
Re: Contamination [Re: LoadedFish]
    #27086631 - 12/13/20 02:30 AM (3 years, 1 month ago)

I appreciate that he said something. But I'm completely new and this site looks like pros are here, yet no one really bothered to post what is happening, only that it does not look good. Is it metabolites or what kind of bacteria. With so many people here all I know that something is not right. Sorry maybe I'm a bit stressed recently... I appreciate advices though


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OfflineShroomhunter40
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Registered: 12/12/20
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Re: Contamination [Re: Eijin88]
    #27086639 - 12/13/20 02:41 AM (3 years, 1 month ago)

Hey guys just off topic , but I have a question or two. I just joined shrommery yesterday what a cool place. Anyhow got my libs at end of season just at the end of last month. Got a couple of great micro d  trips from the fresh libs was excellent. First time then wanting to store  the rest for winter use. Tried a couple of trips since with the stored  frozen from fresh mushrooms but not having the same effect , I got alot  of the same benefits and after effects as before but wasn't the  same. I have read potency diminishes when storing the way I did  as I didn't know this beforehand know now !  I only done same small dose as when fresh picked. My question is should I just up the dose now of my stored  frozen libs to get a decent trip like before?  ANY feed back much appreciated


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OfflineCrackatoa
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Registered: 03/31/19
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Re: Contamination [Re: Shroomhunter40] * 1
    #27086878 - 12/13/20 08:15 AM (3 years, 1 month ago)

People will just tell you it looks bad, wrong, off, whatever they say. Knowing which kind of bacteria or mold isn't going to help. That why we usually just go with a blanket statement of contaminated. I'm going to skip over the maybe that's just what they look like cause I haven't done one of those kits. Sadly 99% of those kits do look like that. Learn agar and you can grow anything you want.


--------------------

Crack's Tek's and Shit



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OfflinePearl
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Registered: 05/28/20
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Last seen: 1 year, 3 months
Re: Contamination [Re: Crackatoa]
    #27087020 - 12/13/20 09:34 AM (3 years, 1 month ago)

Bacterial and way too wet (as said, what kind of bacteria doesn't matter).

As you got a kit and didn't prepare it yourself, it really doesn't matter what went wrong, just that it did. Really the only things I'd imagine you had control over was the amount of spore solution injected or the temperature it was kept at. If you didn't go overboard there with either, then there isn't much else you could have changed.

As it's not doing any harm, might as well let it do it's thing but I wouldn't expect much. Or plant it outside and hope for the best... I stress do not expect much, if anything. I wouldn't go to bulk with this as it'd likely just be a waste of coir/time/effort.

*This is all assuming (as you've given no background info) this was a kit of prepped grain that you inoculated with a spore syringe. If that's the case, then it only started at a crap shoot as spores to grain is risky.


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OfflineEijin88
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Registered: 10/09/20
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Last seen: 2 years, 7 months
Re: Contamination [Re: Pearl]
    #27087040 - 12/13/20 09:48 AM (3 years, 1 month ago)

Outstanding. This is what I loved to hear. I don't want to try to outsmart here as I'm a complete novice,but some info I have found while doing my research about bacteria is that the mycelium may over take the bacteria sometimes and beat it. It's colonised in big percentage now and I'm not smelling anything either. But maybe I'm trying to be too optimistic.

Another info I have seen is that bulk mix like coco coir and vermiculite are more resistant to contamination and putting it in there could do good. Again this is only what I have briefly read and have no idea how much truth is there.

Yes I have purchased a ready substrate, injected b+ and kept it... in unstable temperature. I rent a room in a house share and sometimes the heating been going off during the day,don't know if that what caused the problem here.


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Offlinejcm4620
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Registered: 05/26/19
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Re: Contamination [Re: Eijin88]
    #27087056 - 12/13/20 09:56 AM (3 years, 1 month ago)

kits are a big no no in this hobby.


--------------------
PANAEOLUS FRUITING MADE SIMPLE



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Offlinebunkster
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Registered: 11/21/20
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Last seen: 2 years, 10 months
Re: Contamination [Re: Eijin88]
    #27087281 - 12/13/20 01:31 PM (3 years, 1 month ago)

Quote:

Eijin88 said:
Yes I have purchased a ready substrate, injected b+ and kept it... in unstable temperature.



I would focus on these things if I were you. Look into making and sanitizing your own substrate. Also, make a small incubation tub to keep things warm. If you're relying on the ambient temperature of the room to work with you, things are going to go bad. For my PF tek jars, I made an incubation box like this:

I took the aquarium heater incubation box plan from here, put it in a larger tub, and insulated everything with towels.
Don't get discouraged. Start over with new knowledge and confidence.
Stop sniffing that tub and throw it away.


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OfflineEijin88
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Registered: 10/09/20
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Last seen: 2 years, 7 months
Re: Contamination [Re: jcm4620]
    #27087338 - 12/13/20 02:00 PM (3 years, 1 month ago)

Why?


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OfflineCrackatoa
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Registered: 03/31/19
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Re: Contamination [Re: Eijin88]
    #27087403 - 12/13/20 02:42 PM (3 years, 1 month ago)

Look into making your spawn, substrate is just coir sometimes a little verm with cubes. If you stick with pf tek, there's no need for an incubation box. If you're comfortable so are they. Mid 70s and your good. The warmer your jars are the more prone to things happening. Bacteria and mold like a higher temp. I would suggest reading up on agar and PCing your own grain. You'll need a box but you'll need a SAB. Ensure things are clean before proceeding and you'll have tubs of mushrooms, more than you'll know what to do with.


--------------------

Crack's Tek's and Shit



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OfflineEijin88
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Registered: 10/09/20
Posts: 70
Last seen: 2 years, 7 months
Re: Contamination [Re: Crackatoa]
    #27091521 - 12/16/20 03:18 AM (3 years, 1 month ago)

Is there any reliable tutorial here how to start everything from the scratch? I do have pressure cooker. But I do not know anything how to start.


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OfflineSchemenhaft
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Re: Contamination [Re: Eijin88] * 1
    #27091530 - 12/16/20 03:43 AM (3 years, 1 month ago)

I really recommend this list with TEKs from bodhisatta

Had experience years ago but restarted with these TEKs and a mix of pastywhytes TEKs and maybe also some other TEKs but for pure beginners I'll recommend just 2go with the TEKs of one grower like here bodhisatta, because this is a complete way to success if you take a look at his results and experience.

Later after u did ur first tries, mistakes and with reaching a solid level of knowledge after reading more and more here you can start to change ways, mix TEKs from different people like you prefer, maybe wanna go for personal research and this stuff.


You should read like all TEKs from bodhisatta, for being sure you get a solid knowledge in all important points but if you are a bit impatient for restarting, your basic/important sequence is Spores/syringe to agar, cleaning up Mycel (Agar TEK)  -> Make Spawn TEK -> Clean Agar Mycelium to Grainspawn TEK -> Grain to Monotub TEK

You'll need to read some Side-TEKs from bodhisatta to do these TEKs, like how to build a SAB - Don't skip any steps, if something is unclear, feel free to ask, people here are very helpful and friendly, just make sure u read the TEK and u tried to understand.

Think thats all for now - I really recommend to read most of bodhisattas TEKs at first to make sure u have understand the whole way you go now.


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