Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Bulk Substrate   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Buy Bali Kratom Powder   Bridgetown Botanicals CBD Concentrates   Kraken Kratom Red Vein Kratom

Jump to first unread post Pages: 1 | 2 | 3 | Next >  [ show all ]
InvisiblefahtsterM
Now With 33%More Faht
Male User Gallery


Registered: 06/17/06
Posts: 9,284
Trusted Cultivator
OG Cultivator
Making spore syringes from swabs (I’m using an oven bag**optional) * 8
    #26993868 - 10/20/20 12:45 AM (3 years, 3 months ago)

**I’ll be updating the OP as things progress

INTRODUCTION


So I’ve done this a couple times successfully now (A 3rd time (PE) is in the qt colonizing stage and the 4th time (Texas orange cock) was done 2 days ago and awaiting germination).. I use an oven bag instead of a flow hood or SAB because you can pc an oven bag and I’m pretty anal about my sterile technique, but the process would be the same for a SAB or flowhood.  **the pics of the oven bag construction and procedure will be at the end of this post

The swabs I use are the presterilized kind.. I think I paid $17 for a 100 of them



PROCEDURE AND EXECUTION


One thing I’ve found is that you want your swabs to sit and dry out (in the little package they come in—after you take the spores) for at least a week.. I believe this helps cut back on bacteria because it dries the bacteria out, killing it (non-Endospore producing bacteria anyway).. any swabs that I used right after swabbing didn’t fare as well as ones that sat.

The 1st time I did it, I made a melmak syringe from 1 swab by taking a jelly jar of water (Used about 20mls of water—just enough for the fiber tip to be submerged.. worked ok but I wished I had used more like 30mls) and mashing the swab tip against the jar wall/bottom while submerged.  With the syringe, I made 6, 1/2 pint pf cakes... I was expecting to lose most of the jars due to always hearing that if you make syringes from swabs, they’ll contaminate.  I figured at least one would make it long enough that I could take a leading edge if myc from the colonizing cake (birth early) and clone it. 

4 of the 6 pf cakes germinated and grew out enough that I took a small piece of leading edge myc from a pf cake (birthed it early and not fully colonized) and cloned it to jelly jars of grain for a GLC.  Here’s what those look like.. (I used my Oven bag cloning method)



This was right at the end of my growing season so I only grew out one tub and it reverted (or whatever weirdness melmak was doing around that time—there were a few members that reported non-melmak phenos from known melmak swabs) so I didn’t get that melmak pheno I was looking for but it did tell me that swabs can be made into spore syringes successfully which is a win in my book.  Here’s how that tub turned out (did non-melmak things)



The second time I did it (10/04/20) I made a PE/AA+ swab (swabbed both PE and AA+ to the same swab—I had a mature tub of both varieties and went from one cap to the other.  This is in hopes of creating a cross between the two varieties) into 1 syringe.  This time, when I made the syringe I used two swabs instead of 1... that way I could rub the 2 swabs tips together while under water, further incorporating the varieties’ spores to get a cross and so I didn’t have to mash the tip against the jar wall.

The other thing that I did differently (what I think is the most important part to pull this off) the 2nd time and on, was to do a prewash on the swabs before putting the tips into the water I was going to use for the syringe (I may or may not have gotten the idea from reading Josex’s poke method—can’t remember if I saw his thread before or after I attempted it lol.. either way, josex is a genius and I probably got the idea from him)... so in the oven bag (or SAB or flow hood—which ever you use) I had two jelly jars of sterilized water instead of just the one...

The first one I just dipped the swab tips into and lightly rubbed the tip together to get off any contamination that might have landed on the swab and then went to the other jelly jar that had 30mls of sterilized water and vigorously rubbed the tips together to try and knock the spores off into the water... the second jar is the jar that I pulled from to make my syringes.  (for the 3rd time, I put a little piece of rubber that has tiny nubs on it on the bottom of the second jar of water so that I could rake the swab tips across it to knock the spores off into the water easier.. it worked well)



So far this has proven to be very effective.. with the syringes, I made 4 pf cakes and inoculated a jelly jar of grain so that I could make a GLC to knock up a couple qts to do a test run and see if I get any crosses.  I didn’t expect too much but all four of cakes finished colonizing and they looked great while doing so.. they were super fast and looked really clean and healthy.  They colonized in 14-17 days



With the cakes, I birthed 3 of them into a makeshift FC and left one for experimenting with some invitro ideas



All the cakes fruited (what I believe to be crosses if PE and AA+ at every inoc point) (except the jar one.. still waiting to see what that does fruiting wise)

.  


The 3 cakes dried down to 16.1 grams

I actually fruited that last cake and it put out a monster pea+ fruit that I cloned

.


The GLC grains colonized great too and I used them to knock up 2 qts which also grew just fine and were made into a mini mono which fruited great (cased half of it to hedge my bets lol)

.


I actually put the grains I used for GLC to CV after I let them recolonize after stealing a lot of their myc.. they flushed out already.. dried down to 2.5gm.. the perfect tester dose for me.  Update: I tested out 2 grams of pea+ tea and they’re on par with PE.. maybe not as potent as straight PEs but definitely better than regular cubes.. if PE is a 10, these are a 8.6.. i’d put regular cubes at a 5-6

. . .


The 3rd time is still in colonizing stage, but I made a PE swab into SS and went straight to rye jelly jars.. I made three of them and only put about 1-2 drops of SS to each.  The idea with that being that it reduces the amount of competing sub strains of cubes but also, if there is a nasty floating around in the SS, you reduce the chance that it ends up in all 3 of the jelly jars by reducing the amount of SS each jelly jar gets.  All 3 jars fully colonized without issue... one was used to knock up 4 qts.. they all looked like this 2 days after a shake



Currently 5 days after shake (don’t mind the condensation.. it was pretty chilly in the room this morning—it goes away by afternoon)



Probably give them another day before I spawn them. 

So.. so far, I’m either having extremely good luck or making SS from a swab isn’t as risky as everyone thinks... especially if sterile technique is taken seriously when making the swab and making the SS.  If you think about it, it’s more sterile than making a traditional print (cap sitting on foil—Traditional spore prints are exposed to open air for hours).. the swab comes commercially sterilized and if you do it right, the swab is only exposed to open air for about 30 seconds or even less.. that coupled with the prewash, makes this a viable way to store spores and make them into SS, imo/e. Obviously, swab to agar is a safer way to go about getting myc rolling and I’m not here to sway anyone away from going that route, but as long as you’re smart about it with sterility and go into it thinking you might lose some (make multiple substrates and use as little SS as needed), swab to SS is definitely possible and don’t be afraid to give it a whirl... I’ve found it to be just as reliable as making a syringe from print, if not more so.

And I’m not saying that because my subs didn’t mold out, they’re 100% clean.. I’m positive there’s at least some amount of bacteria just like when making a traditional print into SS.. but for my uses, I’m only trying to get to a first flush so that I can clone.. I don’t need it to go past that, so having a slightly bacterial culture because I made swabs into SS, isn’t a problem for me.  Again, not trying to sway anyone from using agar.. it comes down to me not trusting SAB’s.. I had a horrible experience many years ago where I lost like 12 monos when I was doing g2g in a SAB.. it was right before they were ready to pin too.. it was the WORST.. that’s why I developed the oven bag methods, GLC and my lid designs and since then, it’s been smooth sailing.. I lose very few jars and even less substrates.

MY OVEN BAG CONSTRUCTION AND PROCEDURE.


Let’s get started.. first thing I do is vacuum seal the swab(s).. I ISO the outside of the swab package and the inside of the vacuum pouch and vacuum seal it up... Then I carefully bend the pouch to break the swab sticks about 3” above the swab.. this makes it easier to handle the swabs when I’m dipping them in the water.. better leverage




Then set it aside.  On to making the bag..



Now, a lot of you probably know I love oven bags.. fracking looooOoOoove them.. they’re so versatile.  They can be pc’d.. attachments can be added to them easily.. they’re cheap.. and they’re malleable so I can work with the items placed inside easily with my hands from outside.  I also developed a syringe barrel poly-filled air filter that I hook up to a fish tank bubbler so I can inflate the bag so it’s easier to work with the items inside the bag

The idea is a really simple one... all the items that I need to do either a cloning or spore print to syringe or swab to syringe are secured inside the bag and then pc’d.. once it’s cooled, the swab or print or clone material is transferred to the inside of the bag quickly and carefully (via H2o2 with clone material—and I’m actually working on a method where H2o2 is used to transfer the stem into the bag and once it’s inside the bag, it’s torn open and tissue is taken, so the H2o2 never touches the actual cloned tissue making it 100% clean) through a small opening that was left when the bag was constructed and the bag is sealed back up.. then the process of making the SS is done from outside the bag while the items are secured inside. 

I’m not delusional and don’t think everyone is going to run and make this thing but maybe it’ll give some ppl ideas they didn’t have before... this is long and tedious (trust me I’m using a phone lol) but really it takes like 5 mins to set up a bag once you have all the parts and everything but the bag and zip ties are reusable

This is actually a combination of My oven bag clone tek and the little known IDCAB :lol:  unlike the IDCAB, I’ve actually used this successfully, as I’ve said, 2 times and plan on using it many more.

ITEMS YOU'LL NEED


-  turkey sized oven bags



- Two 2”, 3/4” Dia, pc-able tubing stuffed tight with poly fil and an empty syringe barrel also stuffed tight with poly fil.



-  4” small zip ties



-  tape (I use blue painters tape)

-  empty syringe(s) with plunger (Sometimes I use 1 but you’ll get more SS with 2, obviously)

-  small metal scissors

-  two jelly jars with lids

-  small plate

-  tyvek

BAG ASSEMBLY

1.  Lay your bag out flat and cut just enough off of the sealed corners that the tube of poly will fit through


2.  Attach the tubes with 2 zip ties so that the zip tie heads are opposite each other (this is so if there’s a gap at the zip tie head, having two of them closes each other’s gap—all attachments to bags are done this way)

Do that on both sides


I do this so that steam can enter the bag easily when it’s sealed and the bag doesn’t explode in the pc

3. Cut a small hole and Take your syringe barrel stuffed with poly and attach it to the bag in the middle between the tubing vents... make sure you attach it so that that needle port is on the outside (this is where I plug the hosing from the fish tank bubbler to inflate the bag)


4.  Take some tyvek and make a little pouch that the 2 empty syringes and scissors go into.. this is just to keep those items from poking the bag and keeps them nice and together
.

5.  Take your 2 jelly jars (4oz) and fill them with 30ml of water each... in one of the jars, I use a piece of rubber with nubs on it in one of the jars so that I can rake the swab tips across it to dislodge the spores from the swab.. this is the jar that I’m going to pull the SS from.  The other jar is for the prewash.


6.  Screw the lids on loosely and put them on the plate with your tyvek pouch on top of the jars (or under one of them like in the pic) and place the plate into the oven bag and push it towards the back


7.  Now I seal most of the open bag end by folding the edge over about a 1/2 inch and tape it closed so that all the tape is touching and it’s flat.. I’m making it air tight.  But make sure you leave just enough open so that you can slide the vacuum sealed pouch of swab(s) into the bag


8.  Take that little section that is untapped and roll it up and fold it over (do that a couple times) and zip tie it somewhat loosely.. this just secured the open part so it doesn’t get exposed to air before we’re ready to use it after pc’ing.  Before you do that make sure you push as much air out of the bag as possible


9.  Now I just ball it up as small as I can and make a foul ball (big piece on the bottom that it sits in and a big piece over that) similar to this


10. Pc this for 45 mins (You can probably do less if you want but that’s what I do) if you’re just doing swabs or prints @ 15psi and 1.5 hours if you’re doing a clone to grain and need to sterilize the Jelly jar of grain in the bag.

Now the fun part..

SWAB TO SS (spore solution)


**some pictures might look different because they were taken during a different swab to SS session and since it was only me, I wasn’t able to get some pics of the whole process.. just gonna have to use your imagination at times

1.  Once the pc is cool, take your vacuum sealed swabs and Iso the whole thing.. then take a piece of paper towel and soak it with H2o2.. do the same with another piece and sandwich the vacuum pouch between them.. do this in the area where the transfer into the bag is going to take place.  The idea with this is that it will keep the outside of the pouch clean while you’re setting up the rest of the bag for the transfer


2.  Take the ball out of the Pc and unball it.. lay the bag out so the part you left opening for the transfer is right by your H2o2 pouch sandwich.

3.  Cut the zip tie closing off the transfer opening and unfold and unroll it, keeping the bag as flat as possible... were trying to keep out as much air from inside the bag as possible.

4. Very quickly, slide the pouch into the bag.. again, keeping the opening as flat as possible.

5. After the pouch is in the bag, reroll and fold the opening back up and zip tie it closed.  Here’s the bag after the pouch is inside and the zip tie is on


6. Once the pouch is sealed back up, you need to attach the fish tank bubbler hose to the syringe needle port and turn it on to inflate the bag.. takes about 10 minutes to get the bag to a manageable size
. . .

This is so the bag doesn’t collapse on your work. 

7.  Once the bag is inflated enough, unplug the bubbler.

I don’t have a lot of pics of the next processes because it was only me and I only have two hands lol, but maybe I’ll update later with pics if I have a helper

8.  Take the scissors out of the tyvek pouch (carefully... don’t want to cut the bag) and put your fingers through it from outside the bag.. takes a bit of practice because you need to have slack In the bag in between the scissor holes so when you open the scissors, the bag doesn’t stop you from doing so.


9.  Carefully cut the vacuum sealed pouch (cut the swab pouch at the same time) off around the swab sticks a little above where the break is.. so about right where I’ve drawn this line


10.  Once you’ve cut around the sticks, you can pull that short piece off that has the swab tips in it and the stick ends will be sticking (no pun intended) out.  Put the long pice aside, it’s no longer needed

11.  Now unscrew and remove the lids on your jelly jars.

12.  Pull the swabs out of the cut pouch and take one in each hand (if you have two.. and make sure you hold onto the sticks tightly.. don’t want them to poke through the bag) and dip them in the prewash jar a few times to remove any nasties that might have landed on the surface of the swab.. I rub the swabs together lightly if there’s two.. if only using one, touch the swab tip to the side of the jar wall a couple times

13.  Now take the swab tips to the second jar with the rubber pad in it and dip the swabs in the water and vigorously rub the tips together.. after that, rake the tips Across the surface of the rubber pad that has nubs on it... this helps dislodge the spores from the swab tip.. do this until the swab looks like most/all of the spores have been released into the water

14. Put the swabs aside, we’re done with them.  Take your syringe out of the tyvek pouch and carefully (don’t want the needle to poke the bag—best to not let it touch anything but the water/jar) suck up your SS from the second jar.. like the scissors, you need to take a lot of bag slack between the hand holding the syringe barrel and the hand pulling the plunger otherwise the bag is going to stop you from pulling the plunger all the way up. 

Once the syringe(s) are full, you’re done!  Rip the bag open and retrieve the syringe(s).  Make sure you cut off the reusable pieces on your bag (tubing, syringe barrel) before you throw the bag away.

You can also do traditional prints to SS with this contraption.. all the set up is the same (clean and vacuum seal a foil print) as with swabs, but instead of dipping swabs, you just use one jar of water and cut the foil print out of the vacuum pouch.. then open the print and scrape the spores into the jar of water, either with the syringe needle or with another metal tool/scraper and suck the SS up into the syringe.  Here’s me holding the open print.. you can see the little scraper I pc’d inside the bag (went into the tyvek pouch)


I successfully made AA+ SS from a print this way.. first flush:


Second flush


UPDATE 5/8/21:  I’ve used this a lot since I made this thread.. here’s all the grows that I went from swab —> spore solution..
PE/crs:


PE/RW


PE/pesa


PE/apeu


Even if I go straight to small grain jars and the grains didn’t look as healthy as I wanted, I just top fruit the jars and get clone material or a new swab


Hell.. I’ve even taken swabs that were both broken at the swab tips from shipping and directly injected sterile water into the swab pack, massaged around the water to dislodge spores and sucked the water back out (it has to be a really clean swab/swab pack—thanks, filthyknees) and got these grows from them (apeu):


I know that’s a lot and if you read the whole thing.. thanks :lol:  minus the pc time, it’s all really fast.. 10 minutes to set up the bag and get it ready for the pc and 15 minutes to do the actual transfer and spore collection and I feel much better about doing it than using a SAB, but that’s just me

Faht



Extras: Filter Print Post Top
InvisiblefahtsterM
Now With 33%More Faht
Male User Gallery

Registered: 06/17/06
Posts: 9,284
Trusted Cultivator
OG Cultivator
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: fahtster] * 1
    #27028655 - 11/08/20 01:47 PM (3 years, 3 months ago)

This thread was moved from the user's journal.

Reason:
Go easy on me.. I know it’s quite the contraption :lol:  but hey.. so far it’s working for me :wink:

Faht


Extras: Filter Print Post Top
InvisiblefahtsterM
Now With 33%More Faht
Male User Gallery


Registered: 06/17/06
Posts: 9,284
Trusted Cultivator
OG Cultivator
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: fahtster] * 1
    #27039018 - 11/14/20 03:32 PM (3 years, 3 months ago)

PEA+ F2 syringes made



I’ll be making 4 pf cakes in a couple days

Edit: One cool side effect from rubbing the two swabs together is that when I hold the syringes to the light and look at them, there’s a ton of tiny tiny swab tip particles floating around.. those particles undoubtedly have spores stuck to them.. so they act as a sort of built-in suspension mechanism and I don’t have to worry about free floating spore sticking to the inside of the syringe barrel.  Pretty neat

Faht


Edited by fahtster (11/14/20 03:45 PM)


Extras: Filter Print Post Top
OfflineLogicaL ChaosM
Ascension Energy & Alien UFOs
Male User Gallery


Folding@home Statistics
Registered: 05/12/07
Posts: 69,599
Loc: The Inexpressible... Flag
Last seen: 2 hours, 52 minutes
Trusted Cultivator
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: fahtster]
    #27039034 - 11/14/20 03:37 PM (3 years, 3 months ago)

Really cool Tek! Thanks for moving from your Journal where it belongs now :yesnod:

I have some cottonswabs that I need to use soon and this Tek is definitely helpful to the process :thumbup:


Extras: Filter Print Post Top
InvisiblefahtsterM
Now With 33%More Faht
Male User Gallery


Registered: 06/17/06
Posts: 9,284
Trusted Cultivator
OG Cultivator
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: LogicaL Chaos] * 1
    #27039406 - 11/14/20 07:27 PM (3 years, 3 months ago)

Thanks, pal!


Extras: Filter Print Post Top
OfflineLogicaL ChaosM
Ascension Energy & Alien UFOs
Male User Gallery


Folding@home Statistics
Registered: 05/12/07
Posts: 69,599
Loc: The Inexpressible... Flag
Last seen: 2 hours, 52 minutes
Trusted Cultivator
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: fahtster]
    #27039557 - 11/14/20 08:48 PM (3 years, 3 months ago)

:awesome:

I have three questions. First, I noticed there is blue painters tape around some of the lids. Is this to prevent rusting? Or perhaps to make opening the jars easier?

And your "pre-wash" technique. Have u tried that on spore prints to "wash" them using a swab?


Extras: Filter Print Post Top
InvisiblefahtsterM
Now With 33%More Faht
Male User Gallery


Registered: 06/17/06
Posts: 9,284
Trusted Cultivator
OG Cultivator
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: LogicaL Chaos]
    #27039619 - 11/14/20 09:55 PM (3 years, 3 months ago)

The lids are just the ones I use for making GLC.. https://www.shroomery.org/forums/showflat.php/Cat/0/Number/6886717/an/0/page/0. I switched to painters tape instead of duct tape.  I just had them on there so the jar had a lid in case it spilled.

I haven’t tried that with a print, but I don’t see why it wouldn’t help.. if I’m understanding you correctly lol had some tea earlier

Faht


Extras: Filter Print Post Top
InvisiblefahtsterM
Now With 33%More Faht
Male User Gallery


Registered: 06/17/06
Posts: 9,284
Trusted Cultivator
OG Cultivator
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: fahtster]
    #27050790 - 11/21/20 05:23 PM (3 years, 2 months ago)

I keep getting nice little side effects from going from swab to SS.. so I made the f2 syringes on the 14th and didn’t get around to making the cakes for them until a couple days ago.. because of all the tiny bits of swab material with spores attached that comes off the tips due to the rubbing and ends up in the syringe, the spores germinated while the syringe sat.. the “tiny” bits turned into large bits when I went to inoculate the cakes.. the swab material is cotton and I used tap water so this makes sense. I already have myc growing after two days at all inoc points



While this exciting, it also means that any contamination could have also fed off the cotton while it sat so I’m hoping the prewash did it’s job and that there wasn’t any mold spores mixed in with the mush spores that I grabbed with the swab. The prewash isn’t going to do much for those. So fingers crossed!

Faht


Extras: Filter Print Post Top
InvisiblefahtsterM
Now With 33%More Faht
Male User Gallery


Registered: 06/17/06
Posts: 9,284
Trusted Cultivator
OG Cultivator
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: fahtster]
    #27084923 - 12/12/20 01:10 AM (3 years, 2 months ago)

A little update.. I came up with a new plan to print some pea+ f1 fruits I have just starting to fruit.. gonna print cleanly to the bottom of a jar (I’ll post full procedure later on) and go straight to grain instead of doing cakes to help speed stabilization along.. so I scrapped 3 of the 4 F2 cakes because I needed the jars to do a PE/prfs cross attempt.  I kept the furthest along of the 4 cakes to fruit to get a f3 fruit and it’s just about fully colonized (I think there might be a few knots on the top actually)



I’ve also got a jelly jar of PE/pfrs double swabbed that I did exactly like the pea+ described above that is almost fully colonized and ready to be glc for a couple qts



I’ll keep the updates coming as this moves ahead

Faht


Extras: Filter Print Post Top
InvisibleJosex
#cheat_code
 User Gallery

Registered: 11/13/15
Posts: 8,995
Loc: Flag
Trusted Cultivator
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: fahtster]
    #27084925 - 12/12/20 01:21 AM (3 years, 2 months ago)

:camping:

Here to witness whatever cool madness comes out of this. That clean printing procedure piqued my curiosity, too. :popcorn:


Extras: Filter Print Post Top
OfflineSockadin
Male User Gallery


Registered: 01/03/10
Posts: 7,244
Last seen: 3 months, 8 days
Trusted Cultivator
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: Josex]
    #27084952 - 12/12/20 01:59 AM (3 years, 2 months ago)

That's awesome fahtster! I got to try this technique. Seems really helpful for PE varietys.

Also I did send out some PA+ prints to a few people, mine was a cross between PEU and AA+, but it lost its PEU pheno in the process. I'm working on a new version of it from F3 and will post when I have some results.


Those PEAA+ look really groovy.


Edited by Sockadin (12/12/20 02:00 AM)


Extras: Filter Print Post Top
InvisiblefahtsterM
Now With 33%More Faht
Male User Gallery


Registered: 06/17/06
Posts: 9,284
Trusted Cultivator
OG Cultivator
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: Josex]
    #27084954 - 12/12/20 02:04 AM (3 years, 2 months ago)

Quote:

Josex said:
:camping:

Here to witness whatever cool madness comes out of this. That clean printing procedure piqued my curiosity, too. :popcorn:




Pretty big fan if your PE thread from a couple months ago too, man.  I like the way you think.  I’m gonna do one of your pokes on the inner stem tissue of a fruit on my next PE bin coming up.. figure I can do it fast enough that I can get away with an open air.. figure I can flame the needle, then tear the fruit and have the lc jar real close that I can do the poke and inoculation within a few seconds.. I’m hoping to get enough flesh that going through the SHIP will be ok.. I figure fruit tissue will be denser than agar myc and itll make it through and I can get enough pressure on the plunger with the water to shoot it into the grain water.. I’m gonna make an attempt anyway lol

I forgot I posted a concept of the clean printing here.. thought I only did it an ‘topia.. easy copy paste lol

“Gonna line the inside of a jar with foil (Just the sides and top rim so that the bottom Is exposed)  then take a longish skinny screw and screw it into the stem butt.. then put the cap with screw inside the jar so that the screw head sits on the bottom and the cap rests against the foil.  Then after the spores drop, just flip the jar upside down and hold the foil lip and pull the jar off leaving the cap and foil behind

After thinking about it a bit, I might just zig zag a 1/2” flat (but sitting vertical so the spores can fall to the bottom of the jar) piece of foil on the bottom of the jar so the cap can sit on that instead of using a screw.. gonna play around with both before I attempt the real deal in the oven bag

.

I’m going to put a nut or something similar that’s magnetic and put one of my adjustable airflow lids with SHIP.. I’ll use a magnet to move the nut around from outside the jar to scrape the spores into the water



I’m going to pc the foiled jar with lid and the screw and nut inside an oven bag.. just slip the cap in after the bag cools from the Pc through a little slit in the bag.  Should work like gang busters and give me clean spores that I can go straight to grain with.”

I’ll update with pics of the process when I actually do it here in a few days... and I’m aware of how facking crazy all this oven bag shit is :lol: but I’ve made so damn many of these things over the years that I’m extremely good at constructing them and using them.. I actually updated my oven bag clone method to exclude h2o2.. now I just use sterile water from a syringe to fill the quarantine section and have 2 jelly jars of water I pc inside the bag that I use to rinse the clone material before I put it in the grain jar.. I did that for the first time about a week ago so the jury is still out but the growth looked good before I shook the jars, but I feel really good about the method... I really wanted to get rid of the H2o2 soaking of clone material and I think this going to work great and it was super easy.. the peace of mind alone is priceless.. nothing worse than worrying if your culture survived being soaked in a caustic liquid :lol:



Faht


Extras: Filter Print Post Top
InvisiblefahtsterM
Now With 33%More Faht
Male User Gallery


Registered: 06/17/06
Posts: 9,284
Trusted Cultivator
OG Cultivator
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: Sockadin]
    #27084961 - 12/12/20 02:13 AM (3 years, 2 months ago)

Quote:

Sockadin said:
That's awesome fahtster! I got to try this technique. Seems really helpful for PE varietys.

Also I did send out some PA+ prints to a few people, mine was a cross between PEU and AA+, but it lost its PEU pheno in the process. I'm working on a new version of it from F3 and will post when I have some results.


Those PEAA+ look really groovy.




Nice man!  I sent/sending a few out to tribe members to help stabilize it.. I trust em not to send it out too early and pick only the desirable traits.  They’re out there and I’ve got a nice stock pile of f1 genetics that eventually it’ll be available to all.  I’m putting this clone to grain qts tomorrow/today when I wake up via glc

. .

Culture looks nice and clean



For that one, I transferred the entire intact stem into the oven bag via h2o2 and split the stem and did the transfer while inside the bag.. that was what I thought up before the sterile water idea but it was still using H2o2 and didn’t like it even though the clone tissue never touched the H2o2

Faht


Edited by fahtster (12/12/20 02:21 AM)


Extras: Filter Print Post Top
OfflineSockadin
Male User Gallery


Registered: 01/03/10
Posts: 7,244
Last seen: 3 months, 8 days
Trusted Cultivator
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: fahtster]
    #27084964 - 12/12/20 02:21 AM (3 years, 2 months ago)

For fun here is the PA+ that was a cross between PEU and AA+.


Extras: Filter Print Post Top
InvisiblefahtsterM
Now With 33%More Faht
Male User Gallery


Registered: 06/17/06
Posts: 9,284
Trusted Cultivator
OG Cultivator
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: Sockadin]
    #27084969 - 12/12/20 02:29 AM (3 years, 2 months ago)

Yep.. definitely looks like aa+ and peu had a baby :lol:  did the pin times improve with the aa genes in there?  My peu took even longer than regular PE to pin... and then the flush sucked lol



Could’ve had a shitty culture and/or bacterial etc but I did not like peu at all.. but man, if this keeps working, I’m going to put PE genetics in anything I can get my hands on.. wonder what a PE/peu cross back would “fruit”

Faht


Extras: Filter Print Post Top
OfflineSockadin
Male User Gallery


Registered: 01/03/10
Posts: 7,244
Last seen: 3 months, 8 days
Trusted Cultivator
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: fahtster]
    #27084975 - 12/12/20 02:50 AM (3 years, 2 months ago)

Yeah it wasn't bad if I remember correctly. Was back in June and I haven't had a chance to work with them since.

PE/PEU would be interesting. 6week wait for pins lol.


I want to do a PEU/Z-strain cross this winter. I love how dense and heavy the stems are on the Z, and the Pheno of the PEU.

We should start a Penis Crossing thread and see what everyone comes up with. We can call it the sword fighting thread.


Extras: Filter Print Post Top
InvisiblefahtsterM
Now With 33%More Faht
Male User Gallery


Registered: 06/17/06
Posts: 9,284
Trusted Cultivator
OG Cultivator
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: Sockadin]
    #27084978 - 12/12/20 02:55 AM (3 years, 2 months ago)

:lol: I’m down


Extras: Filter Print Post Top
InvisiblefahtsterM
Now With 33%More Faht
Male User Gallery


Registered: 06/17/06
Posts: 9,284
Trusted Cultivator
OG Cultivator
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: fahtster] * 1
    #27096883 - 12/18/20 11:25 PM (3 years, 1 month ago)

Quote:

“Gonna line the inside of a jar with foil (Just the sides and top rim so that the bottom Is exposed)  then take a longish skinny screw and screw it into the stem butt.. then put the cap with screw inside the jar so that the screw head sits on the bottom and the cap rests against the foil.  Then after the spores drop, just flip the jar upside down and hold the foil lip and pull the jar off leaving the cap and foil behind

After thinking about it a bit, I might just zig zag a 1/2” flat (but sitting vertical so the spores can fall to the bottom of the jar) piece of foil on the bottom of the jar so the cap can sit on that instead of using a screw.. gonna play around with both before I attempt the real deal in the oven bag





Actually did this.. went with the screw screwed into the stem butt.. worked out nicely



Gonna dry it out for a few days and try going straight to grain

Faht


Extras: Filter Print Post Top
OfflineLogicaL ChaosM
Ascension Energy & Alien UFOs
Male User Gallery


Folding@home Statistics
Registered: 05/12/07
Posts: 69,599
Loc: The Inexpressible... Flag
Last seen: 2 hours, 52 minutes
Trusted Cultivator
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: fahtster]
    #27096899 - 12/18/20 11:39 PM (3 years, 1 month ago)

Thats awesome. Im guessing like a wood carpenter screw to elevate it off the glass?


Extras: Filter Print Post Top
InvisiblefahtsterM
Now With 33%More Faht
Male User Gallery


Registered: 06/17/06
Posts: 9,284
Trusted Cultivator
OG Cultivator
Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: LogicaL Chaos] * 1
    #27096902 - 12/18/20 11:44 PM (3 years, 1 month ago)

Yessir

Lined a jar with foil like so


Then put that with lid loosely on the jar on a plate in an oven bag with most of the opening taped up except for enough for the cap to fit through (these are pics from when I made swabs into SS but the set up is the same)
.

Put the screw and other tiny screw (that I put in the jar with the print so I can send magnetically move it around to scrape the spores into the water) in a foil pouch and put it in the bag with the plate and jar and pc’d the bag

Then when it was cool, slid the cap through the little opening and sealed the bag back up and then, from outside the bag with my hands, screwed the screw into the cap stem butt and put it in the jar and loosely put the lid on and waited for the spores to fall... then flipped the jar upside down and pulled the jar off leaving the foil and cap out.. then put the tiny screw in the jar and screwed the lid on.. then cut the bag open and removed the jar. 

Faht


Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 | 3 | Next >  [ show all ]

Shop: North Spore Bulk Substrate   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Buy Bali Kratom Powder   Bridgetown Botanicals CBD Concentrates   Kraken Kratom Red Vein Kratom


Similar ThreadsPosterViewsRepliesLast post
* Oven bag in Oven?!?!?!?!?! wikedanjel 1,982 1 07/08/02 04:33 AM
by mycofile
* Attention Newbies-Make Spore Prints With Foil! Catzeye 22,230 1 02/20/02 01:54 PM
by psilocybinjunkie
* Oven Bags M_R_Shrooms 781 3 06/27/01 01:51 PM
by Scourge
* OVEN BAGS! Sterile 2,572 8 05/15/01 12:11 PM
by Myconoclastic
* my oven bag tek yersodum 2,721 2 08/23/01 04:50 AM
by NDK
* Re: oven bags?????? AldousHuxley 1,208 12 06/06/00 07:06 AM
by Anonymous
* Exploding Oven Bags effwun 2,507 8 10/28/01 11:39 AM
by Anonymous
* Oven Bag Tek G-nome 2,578 4 06/04/01 05:14 PM
by lateshroom

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
4,811 topic views. 19 members, 136 guests and 35 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.037 seconds spending 0.009 seconds on 14 queries.