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Offlinethekickingbird
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Registered: 11/22/19
Posts: 26
Last seen: 2 years, 11 months
Help with syringe inoculation
    #27083598 - 12/11/20 08:30 AM (3 years, 1 month ago)

Hi!

First time using spore syringes to inoculate oat jars.
I have quite experience with agar but since here in Argentina we haven't got the right materials I tried syringe by first time. (We don't have LME here and the agar-agar has a lot of preservatives, tried several brands).


Can you tell me what do you think about this jar. Personally it seems contaminated but maybe it's the first stage of spore grow in this grain (Oat, 24hs soak with gypsum and light coffe, 90min@15psi). I never seen spore grown directly on grains, I always used agar to germinate the spores.

I followed strict sterile technique.





THANKS A LOT IN ADVANCE!


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OfflineSankhara
Trump's lost child
I'm a teapot User Gallery

Registered: 02/11/18
Posts: 546
Loc: Argentina Flag
Last seen: 9 days, 11 hours
Re: Help with syringe inoculation [Re: thekickingbird]
    #27083633 - 12/11/20 09:03 AM (3 years, 1 month ago)

De que me estas hablando!? Soy Argentino y uso agar hace un año y medio sin problemas, agar y harina de arroz integral ambos al 2%

Es imposible saber hasta que tengas colonizacion completa y pases a bulk. Quizá tengas suerte quizá tengas contaminacones.

Saludos vecino!


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Offlinethekickingbird
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Registered: 11/22/19
Posts: 26
Last seen: 2 years, 11 months
Re: Help with syringe inoculation [Re: Sankhara]
    #27084784 - 12/11/20 09:51 PM (3 years, 1 month ago)

Genio! Que marca de agar compras? (La única con la que tuve resultados medianamente buenos tiene la silueta de Argentina en los sobres).

Gracias!


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OfflineSankhara
Trump's lost child
I'm a teapot User Gallery

Registered: 02/11/18
Posts: 546
Loc: Argentina Flag
Last seen: 9 days, 11 hours
Re: Help with syringe inoculation [Re: thekickingbird]
    #27084986 - 12/12/20 03:02 AM (3 years, 1 month ago)

Cualquiera que vendan en la dietetica! Nunca vi ninguna con conservantes. Y la harina de arroz que uso es marca yin yan

Esa de la marca de Argentina funciona barbaro


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Edited by Sankhara (12/12/20 06:23 AM)


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OfflineSankhara
Trump's lost child
I'm a teapot User Gallery

Registered: 02/11/18
Posts: 546
Loc: Argentina Flag
Last seen: 9 days, 11 hours
Re: Help with syringe inoculation [Re: Sankhara]
    #27085005 - 12/12/20 03:58 AM (3 years, 1 month ago)

Si andas corto de guita te recomiendo el TEK para agar en potes de plastico de Josex

Yo uso potes de plastico pp marca coprobras, funcionan excelentes!


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Offlinethekickingbird
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Registered: 11/22/19
Posts: 26
Last seen: 2 years, 11 months
Re: Help with syringe inoculation [Re: Sankhara]
    #27087867 - 12/13/20 07:57 PM (3 years, 1 month ago)

Quote:

Sankhara said:
Si andas corto de guita te recomiendo el TEK para agar en potes de plastico de Josex

Yo uso potes de plastico pp marca coprobras, funcionan excelentes!







Muchísimas gracias!!!!!!!


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