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Shroombitz
Not entitled to a title


Registered: 11/19/20
Posts: 176
Loc: Europe
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Please help with agar
#27081957 - 12/10/20 11:00 AM (3 years, 1 month ago) |
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Hey guys, please help:
Two days ago I have made a transfer because my plate with a clone tissue seemed to be plaqued by trich, it's trich right?
The receiver now seems to be infected again, or am I paranoid? Should I do another transfer? Should I wait a bit longer? Or should I try to cut away the sketchy stuff at 4-5?
Mother plate before transfer:
And receiver two days after transfer:
-------------------- Ask not what your shrooms can do for you, ask what you can do for your shrooms.
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bigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
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Re: Please help with agar [Re: Shroombitz]
#27081990 - 12/10/20 11:26 AM (3 years, 1 month ago) |
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Looks contaminated to me. You could try taking a transfer from the leading edge, maybe around 10 and 2 o'clock, or so and see if it cleans itself up but my money is on contamination. Leave the 4-5 o'clock area alone. But, if it were my plate I would toss it. Cheers, mate!
--------------------
I 5318008 NOT a virgin!
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Shroombitz
Not entitled to a title


Registered: 11/19/20
Posts: 176
Loc: Europe
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Quote:
bigfootscreepyuncl said: Looks contaminated to me. You could try taking a transfer from the leading edge, maybe around 10 and 2 o'clock, or so and see if it cleans itself up but my money is on contamination. Leave the 4-5 o'clock area alone. But, if it were my plate I would toss it. Cheers, mate!
Cheers,
I made another transfer with the tiny tip of the mycelium at 10'o and tossed the oter - So tiny I am not even sure if I got something on the scalpel 
Well if not I guess I can still use the receiver plate ^^
-------------------- Ask not what your shrooms can do for you, ask what you can do for your shrooms.
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Crackatoa
Stranger in a strange land



Registered: 03/31/19
Posts: 5,399
Loc: Over by your Mama's house
Last seen: 1 hour, 35 minutes
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Re: Please help with agar [Re: Shroombitz]
#27082098 - 12/10/20 12:22 PM (3 years, 1 month ago) |
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I've found with trich in a plate. You better make that transfer before it goes green. Once trich goes green it's released it's spores. I would give it another transfer or two and grab an edge as soon as you see an edge, if you want. You can always get a second flush clone.
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Shroombitz
Not entitled to a title


Registered: 11/19/20
Posts: 176
Loc: Europe
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Re: Please help with agar [Re: Crackatoa]
#27082113 - 12/10/20 12:27 PM (3 years, 1 month ago) |
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Quote:
Crackatoa said: I've found with trich in a plate. You better make that transfer before it goes green. Once trich goes green it's released it's spores. I would give it another transfer or two and grab an edge as soon as you see an edge, if you want. You can always get a second flush clone.
Yeah, it looked healthy a few hours back, that trich shit really made some pace
-------------------- Ask not what your shrooms can do for you, ask what you can do for your shrooms.
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Shroombitz
Not entitled to a title


Registered: 11/19/20
Posts: 176
Loc: Europe
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Re: Please help with agar [Re: Shroombitz]
#27087066 - 12/13/20 10:01 AM (3 years, 1 month ago) |
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Two new dishes:
No.1: Spore print to agar by steril swab 8 days ago - It's turning black - Good chances to try a transfer or just toss it?

No.2: Multispore syringe to agar 9 days ago, the solution floated over to the rim - Should I risk a transfer or just let it keep on growing like that?
-------------------- Ask not what your shrooms can do for you, ask what you can do for your shrooms.
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Lemgrub



Registered: 04/02/18
Posts: 589
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Re: Please help with agar [Re: Shroombitz]
#27087110 - 12/13/20 10:50 AM (3 years, 1 month ago) |
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Looks like a contaminant mold in both of those, but fresh mycelia from spores can look off. Try back lighting the plates so the leading edge is more apparent.
-------------------- We getting that pirate bay, alien shishkebab cordycep money

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