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InvisibleShroombitz
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Registered: 11/19/20
Posts: 176
Loc: Europe
Please help with agar
    #27081957 - 12/10/20 11:00 AM (3 years, 1 month ago)

Hey guys, please help:

Two days ago I have made a transfer because my plate with a clone tissue seemed to be plaqued by trich, it's trich right?

The receiver now seems to be infected again, or am I paranoid?
Should I do another transfer? Should I wait a bit longer? Or should I try to cut away the sketchy stuff at 4-5? 

Mother plate before transfer:


And receiver two days after transfer:


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Ask not what your shrooms can do for you, ask what you can do for your shrooms.


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Invisiblebigfootscreepyuncl
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Registered: 11/15/20
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Re: Please help with agar [Re: Shroombitz]
    #27081990 - 12/10/20 11:26 AM (3 years, 1 month ago)

Looks contaminated to me. You could try taking a transfer from the leading edge, maybe around 10 and 2 o'clock, or so and see if it cleans itself up but my money is on contamination. Leave the 4-5 o'clock area alone. But, if it were my plate I would toss it. Cheers, mate!


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InvisibleShroombitz
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Re: Please help with agar [Re: bigfootscreepyuncl]
    #27082065 - 12/10/20 12:09 PM (3 years, 1 month ago)

Quote:

bigfootscreepyuncl said:
Looks contaminated to me. You could try taking a transfer from the leading edge, maybe around 10 and 2 o'clock, or so and see if it cleans itself up but my money is on contamination. Leave the 4-5 o'clock area alone. But, if it were my plate I would toss it. Cheers, mate!




Cheers,

I made another transfer with the tiny tip of the mycelium at 10'o and tossed the oter - So tiny I am not even sure if I got something on the scalpel :grin:

Well if not I guess I can still use the receiver plate ^^


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Ask not what your shrooms can do for you, ask what you can do for your shrooms.


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OfflineCrackatoa
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Re: Please help with agar [Re: Shroombitz]
    #27082098 - 12/10/20 12:22 PM (3 years, 1 month ago)

I've found with trich in a plate. You better make that transfer before it goes green. Once trich goes green it's released it's spores. I would give it another transfer or two and grab an edge as soon as you see an edge, if you want. You can always get a second flush clone.


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InvisibleShroombitz
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Registered: 11/19/20
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Re: Please help with agar [Re: Crackatoa]
    #27082113 - 12/10/20 12:27 PM (3 years, 1 month ago)

Quote:

Crackatoa said:
I've found with trich in a plate. You better make that transfer before it goes green. Once trich goes green it's released it's spores. I would give it another transfer or two and grab an edge as soon as you see an edge, if you want. You can always get a second flush clone.




Yeah, it looked healthy a few hours back, that trich shit really made some pace


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Ask not what your shrooms can do for you, ask what you can do for your shrooms.


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InvisibleShroombitz
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Re: Please help with agar [Re: Shroombitz]
    #27087066 - 12/13/20 10:01 AM (3 years, 1 month ago)

Two new dishes:

No.1: Spore print to agar by steril swab 8 days ago - It's turning black - Good chances to try a transfer or just toss it?


No.2: Multispore syringe to agar 9 days ago, the solution floated over to the rim - Should I risk a transfer or just let it keep on growing like that?


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Ask not what your shrooms can do for you, ask what you can do for your shrooms.


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InvisibleLemgrub
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Registered: 04/02/18
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Re: Please help with agar [Re: Shroombitz]
    #27087110 - 12/13/20 10:50 AM (3 years, 1 month ago)

Looks like a contaminant mold in both of those, but fresh mycelia from spores can look off. Try back lighting the plates so the leading edge is more apparent.


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