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OfflineGoldenDude
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Grain spawn bottom not colonizing
    #27078223 - 12/08/20 05:49 AM (3 years, 2 months ago)

Hello,

I have a grain spawn, which populated very well above bottom inch of the bag.
So far i have injected spores to the grain, waited for it to populate 70%+.
Then I mixed the bag.
Left it to fully populate.
After a while - 7days or so after mixing, my bags look like that:


Is something wrong? Should I just wait?
Should I do anything to help populate the bottom?

Thanks dear friends :smile:


Edited by GoldenDude (12/08/20 05:49 AM)


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Offlineanatomality
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Re: Grain spawn bottom not colonizing *DELETED* [Re: GoldenDude]
    #27078229 - 12/08/20 05:55 AM (3 years, 2 months ago)

Post deleted by anatomality

Reason for deletion: better answers below


--------------------
“The strength of a person's spirit would then be measured by how much 'truth' he could tolerate, or more precisely, to what extent he needs to have it diluted, disguised, sweetened, muted, falsified.”


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OfflineGoldenDude
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Re: Grain spawn bottom not colonizing [Re: anatomality]
    #27078230 - 12/08/20 05:58 AM (3 years, 2 months ago)

Do you think, perhaps i could just use the bottom , colonized grain, and toss out the bottom? Instead of throwing out the whole bag?


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Offlineanatomality
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Re: Grain spawn bottom not colonizing *DELETED* [Re: GoldenDude]
    #27078347 - 12/08/20 08:07 AM (3 years, 2 months ago)

Post deleted by anatomality

Reason for deletion: Better answers below


--------------------
“The strength of a person's spirit would then be measured by how much 'truth' he could tolerate, or more precisely, to what extent he needs to have it diluted, disguised, sweetened, muted, falsified.”


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OfflineGreens21
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Re: Grain spawn bottom not colonizing [Re: anatomality] * 2
    #27079032 - 12/08/20 04:30 PM (3 years, 2 months ago)

This belongs in Mush Cult, but none of that mycelium is usable. See how it’s thick and creamy, like melted ice cream? That’s a bacterial infection. Mycelium should be fuzzy



If a bag doesn’t recover (fully colonize) quickly (within 2-3 days) after shaking/mixing, it’s a sure sign of contamination (or too-dry grains, which obviously is not the problem here). Fermented grains will colonize just fine; the mycelium has created a “wall” to try to keep further contaminants out of the mycelial structure, which is why it’s no longer colonizing.

Did you purchase these grow bags pre-sterilized? You’ll have much better success rates making your own and doing it properly.

Better luck on your next attempt


--------------------
I've been trying to justify you

In the end I will just defy you







Edited by Greens21 (12/08/20 04:44 PM)


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OfflineGoldenDude
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Re: Grain spawn bottom not colonizing [Re: Greens21]
    #27079879 - 12/09/20 03:57 AM (3 years, 2 months ago)

Quote:

Greens21 said:
This belongs in Mush Cult, but none of that mycelium is usable. See how it’s thick and creamy, like melted ice cream? That’s a bacterial infection. Mycelium should be fuzzy



If a bag doesn’t recover (fully colonize) quickly (within 2-3 days) after shaking/mixing, it’s a sure sign of contamination (or too-dry grains, which obviously is not the problem here). Fermented grains will colonize just fine; the mycelium has created a “wall” to try to keep further contaminants out of the mycelial structure, which is why it’s no longer colonizing.

Did you purchase these grow bags pre-sterilized? You’ll have much better success rates making your own and doing it properly.

Better luck on your next attempt




I have bought these bags pre-sterilized.

Also, I have already shaken these bags once, do you think its ok to shake them again? For the 2nd time?

Can you please refer me to tek you prefer for preparing the grain spawn?


Edited by GoldenDude (12/09/20 03:58 AM)


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InvisiblemushboyMDiscord
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Re: Grain spawn bottom not colonizing (moved) [Re: GoldenDude]
    #27080021 - 12/09/20 07:36 AM (3 years, 2 months ago)

This thread was moved from Psychedelic Medicine, Research & Microdosing .

Reason:
:awedance:


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OfflineThe Mycologist
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Re: Grain spawn bottom not colonizing [Re: GoldenDude] * 1
    #27080045 - 12/09/20 08:06 AM (3 years, 2 months ago)

That spawn isnt healthy. I would just case the bags and top fruit.

Spawning may end the chances of getting any fruits.


--------------------
"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
:acidfire::tmckenna:


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OfflineFailboat
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Re: Grain spawn bottom not colonizing [Re: The Mycologist]
    #27080134 - 12/09/20 09:23 AM (3 years, 2 months ago)

:whathesaid:


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OfflineGoldenDude
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Re: Grain spawn bottom not colonizing [Re: The Mycologist]
    #27080244 - 12/09/20 10:40 AM (3 years, 2 months ago)

Quote:

The Mycologist said:
That spawn isnt healthy. I would just case the bags and top fruit.

Spawning may end the chances of getting any fruits.




Can you please re-iterate your sentences?

I am not sure what do you mean.

Can you please explain what you mean by:
1. I would just case the bags and top fruit.
2. Spawning may end the chances of getting any fruits.

Thank you very much :smile:


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OfflineGoldenDude
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Re: Grain spawn bottom not colonizing [Re: The Mycologist]
    #27080252 - 12/09/20 10:42 AM (3 years, 2 months ago)

Quote:

The Mycologist said:
That spawn isnt healthy. I would just case the bags and top fruit.

Spawning may end the chances of getting any fruits.




Do you think mixing the spawn again, for the 2nd time, could help the colonization and rescue my grain spawn?


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OfflineFailboat
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Re: Grain spawn bottom not colonizing [Re: GoldenDude] * 1
    #27080254 - 12/09/20 10:43 AM (3 years, 2 months ago)

He's saying to just open the bag from the top and apply a casing. Then just let it fruit from the top. I would put the open cased bag inside a tub.

DO NOT MIX THAT BAG AGAIN.


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OfflineThe Mycologist
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Re: Grain spawn bottom not colonizing [Re: GoldenDude]
    #27080291 - 12/09/20 11:04 AM (3 years, 2 months ago)

Quote:

Quirkmeister92 said:
He's saying to just open the bag from the top and apply a casing. Then just let it fruit from the top. I would put the open cased bag inside a tub.

DO NOT MIX THAT BAG AGAIN.




:whathesaid:

Case means put something on top of the grain. Traditionally casing has a certain ph to encourage fruiting, but with psilocybe cubensis, a faux casing of coir works just fine.

Spawning is the act of opening up the grains and mixing them with coir

I advise you keep waiting to see if more colonizes, and when you give up on it reaching 100% colonization, just put a layer of coir on top of the grains and cross your fingers.

It may contam but doing this is a better chance then breaking it up into coir.


--------------------
"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
:acidfire::tmckenna:


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OfflineMessiah of Savants
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Re: Grain spawn bottom not colonizing [Re: The Mycologist]
    #27080351 - 12/09/20 11:41 AM (3 years, 2 months ago)

Do yourself a favor and save yourself some time. Throw that bag away.

There are times you can try spawning bacterial jars and get fruits, but I don't think you are going to get anything from that bag.


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OfflineThe Mycologist
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Re: Grain spawn bottom not colonizing [Re: Messiah of Savants] * 1
    #27080362 - 12/09/20 11:44 AM (3 years, 2 months ago)

:whathesaid: Definitely has truth in it too


--------------------
"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
:acidfire::tmckenna:


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InvisibleJosex
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Re: Grain spawn bottom not colonizing [Re: The Mycologist]
    #27080383 - 12/09/20 11:58 AM (3 years, 2 months ago)

Yep that's toast.


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OfflineFailboat
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Re: Grain spawn bottom not colonizing [Re: Josex]
    #27081299 - 12/09/20 10:09 PM (3 years, 2 months ago)

I would only keep it if you were absolutely desperate to get spores/tissue samples to continue working.


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OfflineGoldenDude
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Re: Grain spawn bottom not colonizing [Re: Failboat]
    #27081905 - 12/10/20 10:13 AM (3 years, 2 months ago)

So i kept these bags for past 6 weeks or so. I have bought them, as i dont have any sterilizer.

I put a lot of effort and money into these bags.

What would happen if i would mix it with bulk substrate?
Why I should not mix the bag for the 2nd time?

Why did they turn bad?
How can I know next set of grains will not end up the same?

I kept them in a dark wardrobe with temps between 19.5'C and 22'C - sometimes temp grew to 28 when the colonization was most rapid.

Lots of questions, I know, although I think these are valid questions? This way I will learn.

Thank you and peace :smile:


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OfflineGreens21
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Re: Grain spawn bottom not colonizing [Re: GoldenDude]
    #27081943 - 12/10/20 10:47 AM (3 years, 2 months ago)

Either the bags themselves were contaminated when you got them, or the syringe was contaminated (though syringes are pretty much always contaminated, which is why most people here recommend growing from agar instead). Your bags look super wet, is there that much water in them when they arrive? Or does it develop that much moisture during colonization?

You’re free to try to get those bags to do something, but they most likely won’t, whatever you do with them. So, either way, you should get your next grow started rather than waiting to see what these do. You asked what would happen if you spawned these to bulk, and the answer is probably nothing. The mycelium is too busy fighting off whatever bacteria is in there to try to spend energy growing and colonizing, so you’ll just end up wasting time and substrate.

If you absolutely have to use a spore syringe to inoculate the next set of bags, keep them in complete blackness for pretty much all of colonization. Wrap them in foil (making sure to leave the gas exchange port uncovered) and stick them in a box or a black/dark storage tub, and don’t check on them, at all, for at least 10 days. While mycelium benefits from light exposure, it doesn’t need the light, and contaminants do. By taking away a source of energy for contaminants you’ll allow your mycelium a chance to get a strong head start/foothold over any contaminants that may exist. Almost every grow I’ve done has been inoculating grain from syringe and I’ve lost zero jars to contamination in 13 years, though I also make my own grain jars.


--------------------
I've been trying to justify you

In the end I will just defy you







Edited by Greens21 (12/10/20 10:54 AM)


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OfflineThe Mycologist
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Re: Grain spawn bottom not colonizing [Re: Greens21]
    #27082145 - 12/10/20 12:45 PM (3 years, 2 months ago)

Mushrooms benefit from light at all times.

We dont try to beat contams we try to not let them in to start with.


--------------------
"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
:acidfire::tmckenna:


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