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Offlinenogoodnamesleft
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Registered: 09/19/20
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Agar gel strength effect on culture morphology
    #27081158 - 12/09/20 08:23 PM (3 years, 1 month ago)

Good evening Shroomery

I am currently using Technical Grade Agar from

https://phytotechlab.com/agar-technical-grade.html

It has a gel strength of >900g/cm

I noticed the Living Jin Agar powder that d3monic uses has a gel strength of 700g/cm2

I have a very hard time getting rhizomorphic cultures.

I am curious if the harder gel strength at the same concentration can effect overall culture morphology

Thank you


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OfflineCatsLoveHouseMusic
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Re: Agar gel strength effect on culture morphology [Re: nogoodnamesleft] * 1
    #27081225 - 12/09/20 09:02 PM (3 years, 1 month ago)

I’m also interested so bump. I forgot but I’ve read about this before but I don’t remember. I’ve always done 8G agar 8gram LME to 400-420ml


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Offlinenogoodnamesleft
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Re: Agar gel strength effect on culture morphology [Re: nogoodnamesleft]
    #27113697 - 12/29/20 08:09 AM (3 years, 30 days ago)

To update -

I have been making the dishes too weak both with the agar and nutrients. I increased the agar and nutrients from 1% to 2% and included nutritional yeast @ 1 gram

I also prepared PDYA plates

I transferred growth from plates I was struggling with but had clean mycelium as well as a culture that I got from a friend to plates on these new recipes.

I'll be back in a week with photos


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