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InvisibleReaperZero
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1st hybrid attempt, please help! (AA+ / Azurescens)
    #27078295 - 12/08/20 07:20 AM (3 years, 3 months ago)

I took my first stab at crossing 2 strains. AA+ & Azurescens. I'm not sure if there's a 3rd new culture growing or what, & if so is my time limited to take samples? Any help would be greatly appreciated.

These first few photos are from yesterday but i didnt take any with the lid off (they're blurry/awful), bear with me!





These were taken today. I didnt get a chance to look at the cultures for several hours, when i looked at it this morning the cultures were even closer. The circled part is the part in question but it looks like the culture its emerging from?









No agents were used for this experiment, in future trials i plan on trying niacin (first), then possibly considering snake venom if I ever get my hands on semilanceata spores.

Lastly, THIS is the embarassing bit but.... I foolishly didn't bother marking which was which, is anyone able to tell by the mycelium? Thanks in advance folks!


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-Zero-

Edited by ReaperZero (12/08/20 07:32 AM)

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InvisiblebodhisattaMDiscordReddit
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Re: 1st hybrid attempt, please help! (AA+ / Azurescens) [Re: ReaperZero]
    #27078938 - 12/08/20 03:25 PM (3 years, 3 months ago)

AA+ is a cubensis variety.

Ps. Azurescens is a completely different species.

This has no chance of working. You need two species more closely related and even then you're honestly probably in no position to cross species.

You can cross varieties of the same species. They're not hybrids though sometimes they're called that


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InvisibleReaperZero
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Re: 1st hybrid attempt, please help! (AA+ / Azurescens) [Re: bodhisatta]
    #27079662 - 12/08/20 10:46 PM (3 years, 3 months ago)

Ya, i know this attempt is about as farfetched as it gets (like trying to cross a banana with a dog). I also have a plate with B+ / Panaeolus Tropicalis & another with Azurescens / Cyanescens (none of those have a zone of inhibition formed yet though). Planning on trying other combinations once i make more agar , including some cube/cube attempts which is probably where i should've STARTED, but it's the least 'interesting' to me. My 'hope' with the cube/azure madness is simple. I'm mostly wanting to pass on albino/leucistic traits to azurescens (if this is even possible im sure theres a far more logical approach?). These experiments are honestly just 'practice' though, the main species i  wanna cross is semilanceata (elusive son of a gun with vendors), (with a warmer growing species maybe). For future trials i plan on using bigger containers for more growing room, MARKING the strains & 'planning' on total failure & just using the plates as agar dishes for whatever 2 species are on it. I'm going to try MEA, cornmeal agar, & both with nicotinic acid added. I'm definitely trying stuff far beyond my comprehension / understanding levels but that isn't going to stop me. For now it's just 'madness' but if the day comes where i have the proper ingredients (semilanceata, venom perhaps, & whichever species i wanna try crossing it with) I might try to enlist in some help. I set very high goals for myself in this hobby, the main 2 being crossing any 2 notable species (not a varietal hybrid) & the other being successful indoor semilanceata growing. Without ridiculous challenges / mad science it just wouldn't be as fun to me.


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-Zero-

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Offlinejomanda1990
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Re: 1st hybrid attempt, please help! (AA+ / Azurescens) [Re: ReaperZero]
    #27080176 - 12/09/20 09:56 AM (3 years, 3 months ago)

For the hybrid to ever occur it should be possible for both monokaryon of the different species to couple. This is not possible between different species because of the proteins that have to be interlocked and the chemical receptors on the cell membrane that have to be activated in what's likely to be a complex chemical signaling cascade. Otherwise, there won't be genetic exchange between both species and thus no hybrid.
Sorry but this seems too unfeasible IMO. As Bod suggests, you should at least try with species that are overly genetically similar to begin with.
On the other hand, if you want to cross stuff, crossing cubensis varieties WILL give you actual results.

Anyhow, good luck


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