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OfflineSockadin
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Re: What happened? [Re: verytastycheese]
    #27136816 - 01/09/21 06:32 AM (14 days, 15 hours ago)

Quote:

verytastycheese said:
I'm on the edge of my seat!





Looking good. Can I ask what the plastic bags over your jars are for?


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Offlineverytastycheese
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Re: What happened? [Re: Sockadin]
    #27137197 - 01/09/21 11:29 AM (14 days, 10 hours ago)

Quote:

Professor X said:
You can use tiny amounts per jar. I use agushy 2oz slime containers from Amazon as plates and do 14 quarts per plate.




Wow great! I was doing 8 per plate plus transfers to 2 new plates.
Ended up with 16 masters all looking amazing. Making 2 full size bins today and going to g2g the rest and make some more masters in the meantime. Thinking the least G2G cycles the better.

Quote:

Sockadin said:
Quote:

verytastycheese said:
I'm on the edge of my seat!





Looking good. Can I ask what the plastic bags over your jars are for?




I live in a very dry climate and can't seem to get the humidity of my room above 40% even with a humidifier using 4L of water per day. I usually find that the tops of the jars dry out before fully colonizing and any uncolonized grains are a contam risk. The bags act as humidity tents and seem to have solved the issue for this round at least :smile:


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Offlineverytastycheese
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Re: What happened? [Re: verytastycheese]
    #27146348 - 01/13/21 07:44 PM (10 days, 2 hours ago)

So 12 days in and I'm back to scratching my head as to whether these are going to pin.


Also here's what the previous genetics finally did on the petri dish. Is this a fruit?? I never once saw what looked like a pin.



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InvisibleJosex
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Re: What happened? [Re: verytastycheese]
    #27146368 - 01/13/21 07:54 PM (10 days, 2 hours ago)

12 days is not a lot yet, be patient it's looking good. I think you're going to start seeing knots very soon. Next time though, you can add a layer of substrate at spawning, it helps maintain good surface conditions later on.



It looks like a blob, some genetics are prone to blob but it's pretty rare to find one that does it on agar except for some PE varieties. I wouldn't chalk it up to contamination or anything like that becasue the plates you've been posting look very clean.

The fact that it blobbed on agar doesn't necessarily mean that it will do the same in a tub, though.


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Offlineverytastycheese
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Re: What happened? [Re: Josex]
    #27146432 - 01/13/21 08:39 PM (10 days, 1 hour ago)

Quote:

Josex said:
12 days is not a lot yet, be patient it's looking good. I think you're going to start seeing knots very soon. Next time though, you can add a layer of substrate at spawning, it helps maintain good surface conditions later on.




Okay sounds good. The waiting is the hardest part.
Quote:


It looks like a blob, some genetics are prone to blob but it's pretty rare to find one that does it on agar except for some PE varieties. I wouldn't chalk it up to contamination or anything like that becasue the plates you've been posting look very clean.

The fact that it blobbed on agar doesn't necessarily mean that it will do the same in a tub, though.




Interesting, alright. Lots of metabolites on the blob but I suppose that does happen over time even without contams? It's the old genetics, a sibling of what I'm playing with now so it shouldn't matter anyway.


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InvisibleJosex
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Re: What happened? [Re: verytastycheese]
    #27146465 - 01/13/21 09:00 PM (10 days, 1 hour ago)

yea tbh I've never seen a blob on agar producing metabolites but blobs can produce metabolites even without the presence of contamination. Do you happen to have an earlier pic of that plate, this is interesting.


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Offlineverytastycheese
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Re: What happened? [Re: Josex]
    #27146473 - 01/13/21 09:05 PM (10 days, 1 hour ago)

Yep that's this plate:



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InvisibleJosex
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Re: What happened? [Re: verytastycheese]
    #27146486 - 01/13/21 09:12 PM (10 days, 53 minutes ago)

That plate looks fine in my book. Been taking a look at the pics of your spawn and dem jars are looking pretty damn sexy too, you're on your way to a lot of shrooms if you ask me. :hehehe:


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Offlineverytastycheese
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Re: What happened? [Re: Josex]
    #27147556 - 01/14/21 01:32 PM (9 days, 8 hours ago)

These are the 2nd batch of jars. I didn't bother shaking them at 30% (except a few) because they were so far along by the time I looked. Do you think it's better for myc to not be broken up so many times in it's lifecycle?

Anyway they look good in the jars for 7 days after G2G :smile:


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OnlineCrackatoaS
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Re: What happened? [Re: verytastycheese]
    #27147635 - 01/14/21 02:14 PM (9 days, 7 hours ago)

If the mycelium is healthy it'll bounce back.


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InvisibleJosex
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Re: What happened? [Re: Crackatoa]
    #27148784 - 01/15/21 12:32 AM (8 days, 21 hours ago)

Yeah, shake em as many times as needed. If you have a lot of myc covering only one part of the jar, shake it. If the whole jar seems to be mostly colonized but there's a patch that isn't, shake it. If you're bored, shake it. If you are unsure about the health of your spawn, wait until 100% colonized and shake it, that's gonna tell you things by the way it recovers.

Talking of invitro blobs that produce metabolites without there being contamination, here's a pic of a Brazil culture that I took today from a BRF puck:


I know you have to take my word for it it isn't contaminated, so yeah it isn't lol it's just overgrown and wants to fruit but it isn't liking so much nutrition.


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InvisibleJosex
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Re: What happened? [Re: verytastycheese]
    #27148795 - 01/15/21 12:43 AM (8 days, 21 hours ago)




In fact I'd have shook the fuck out of those jar right then and there.

Your shit always looks good dude, dem shroom gods are smiling upon thee.
:pope:


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Offlineverytastycheese
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Re: What happened? [Re: Josex]
    #27151777 - 01/16/21 06:25 PM (7 days, 3 hours ago)

Quote:

Josex said:



In fact I'd have shook the fuck out of those jar right then and there.

Your shit always looks good dude, dem shroom gods are smiling upon thee.
:pope:




Well it's about time they smile! I spent a solid year going at this producing pounds and pounds of trich!
I'm not immediately ready to spawn those jars so I'm in no rush for them to finish up, but good to know you have no issues shaking at any time for any reason.

But look! Finally! A solitary pin. Better than I've seen in a while.

Now I just have to figure out how we bulk hydrate coir for steam pasteurization. Are pillowcases still the go-to?


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OnlineCrackatoaS
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Re: What happened? [Re: verytastycheese]
    #27152337 - 01/17/21 12:15 AM (6 days, 21 hours ago)

Yeah, I shake around 30% and around 70%. Sometimes I just shake once. Search for bucket tek, no pasteurization or pillowcases unless you're using poo.


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Offlineverytastycheese
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Re: What happened? [Re: Crackatoa]
    #27156427 - 01/19/21 12:32 AM (4 days, 21 hours ago)

A wild contam appears?




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OfflineRamJam
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Re: What happened? [Re: verytastycheese]
    #27156633 - 01/19/21 04:22 AM (4 days, 17 hours ago)

Bummerrrrrr


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Offlineverytastycheese
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Re: What happened? [Re: RamJam]
    #27159939 - 01/20/21 06:59 PM (3 days, 3 hours ago)

Still inconsequential, at least I know the genetics will pin. But back to the theory that if you get contams before first flush it must be in your spawn? No chance it was introduced by an imperfect spawning or pasteurization of substrate? What I'm learning here is I probably need to shake my jars more to potentially reveal contams?

Moreover, I should move to liquid. I'll be reading up on your tek Josex.

Here's the jars 3 days after I shook them. At what point would you call them fully colonized and ready to spawn?




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Offlinecalmtitty
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Re: What happened? [Re: verytastycheese]
    #27160072 - 01/20/21 08:04 PM (3 days, 2 hours ago)

If you are using coir/verm as your substrate I highly doubt the contamination came from there, no matter how you prepped it. I know that other subs like straw or manure need to be properly pasteurized.

I'm still new to growing, but I have been shaking all of my jars at 90% or even 100% just to make sure they are clean before I bother spawning them. I don't see how it could hurt and I'm not in a rush to do anything.

From what I understand, clean spawn will never contaminate your grow.


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OfflineProfessor X
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Re: What happened? [Re: calmtitty]
    #27160076 - 01/20/21 08:09 PM (3 days, 1 hour ago)

If it's coir verm you can use cold tap water to prep. Yes the mold is in your jars, could be in your culture.


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Offlineverytastycheese
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Re: What happened? [Re: Professor X]
    #27160290 - 01/20/21 10:20 PM (2 days, 23 hours ago)

Is the gypsum relevant? I actually omitted it in my latest monotub run so we'll see if that makes a difference.

If it's in the culture it's the most ninja shit anyone has seen. I'm confident I have clean plates. I'm fairly confident I currently have clean jars. It's possible that a contam got in dropping the wedge into that particular master jar. Either way that doesn't affect the rest of my jars. Hopefully it was the only one. I'll shake them more from now on!


Edited by verytastycheese (01/20/21 10:26 PM)


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