|
 Josex
Axenic Cheater
Registered: 11/13/15
Posts: 7,207
Loc: 
|
|
Non-fruiting genetics are extremely rare, to the point I doubt you'd ever encounter them in your life.
99,999999% of the times, tubs not fruiting are caused by contamination and/or bad conditions. So you shouldn't trip over that, neither should you trip over your plates because they're looking fantastic.
Color blue is good for spotting contams, as well as no food color at all, no sweat there.
Don't waste your time adding peroxide to your agar, it's going to turn into plain water when sterilized. We use agar to let any existing contamination show up, so we can transfer away from it, so this is not about using bandaids.
Work on your technique inoculating grain jars because it's plain as day you've been screwing up there. Inoculating with little agar wedges helps a lot preventing contams from technique. I would advise against dropping a whole plate per jar, as this is more likely to let in contams.
Those plates are ready for grain right now, no need to let them grow further if you don't want to.
|
 verytastycheese
Tripmaster
Registered: 11/11/14
Posts: 91
Last seen: 20 minutes, 17 seconds
|
 Re: What happened?  [Re: Josex]
#27092387 - 12/16/20 05:18 PM (1 month, 7 days ago) |
|
|
Josex thanks for the advice!
From what I was reading RR and others would put 1/3 of a plate in their grain jars. Is that excessive? Are you saying wedges like I would transfer or like pizza slices? Do you by chance have a link I could read up on to brush up on that technique? I finally have my LFH so that should help I figure 
|
Josex
Axenic Cheater
Registered: 11/13/15
Posts: 7,207
Loc:
|
|
Any size wedge would do. I like little triangular wedges, maybe like twice as big as the wedges you are currently using for agar to agar transfers.
Then, I barely crack open the lid of the grain jar and I use the (sterile) inner rim of the jar to get the wedge off the blade and immediately close the lid again.
It's all done very swiftly, this way you ensure you limit the time you expose the media (grains) to a minimum.
Another advantage is, you can inoculate a lot of jars with one single plate by using small wedges.
You can shake when the wedge grows out about an inch in diameter, then shake again at 30% and maybe a last shake later if needed.
|
Crackatoa
Stranger in a strange land
Registered: 04/01/19
Posts: 3,031
Loc: Over by your Mama's house 
Last seen: 11 minutes, 39 seconds
|
Re: What happened? [Re: Josex]
#27092725 - 12/16/20 08:33 PM (1 month, 7 days ago) |
|
|
 Josex for the perfect explanation.
|
verytastycheese
Tripmaster
Registered: 11/11/14
Posts: 91
Last seen: 20 minutes, 17 seconds
|
|
Update: I followed your method and knocked up 8 jars from 2 plates along with child plates. The jars are growing as expected and I'm waiting for a little over quarter size to shake.
The jar I dropped the whole agar plate in after transfers did in fact contam out after the first shake. I'll stick with dropping wedges in. Thanks for that 
The plate I took the transfers from looked badass after a couple days so:
|
Josex
Axenic Cheater
Registered: 11/13/15
Posts: 7,207
Loc:
|
|
That plate is absolutely boss man, you'll look back some day when you're a pro and realize it isn't easy to come across a culture looking that nice.
And to think some people were shitting on your plates in this very thread lol.
Please keep us updated on this grow, I for one would like to see it to the end.
|
verytastycheese
Tripmaster
Registered: 11/11/14
Posts: 91
Last seen: 20 minutes, 17 seconds
|
Re: What happened? [Re: Josex]
#27099981 - 12/20/20 11:04 PM (1 month, 2 days ago) |
|
|
Damn you're quick on the draw Josex. Replied within 3 mins! 
Thanks so much! Considering what you've said, how should I go about storing this culture? I have a mini fridge in the lab that I'm putting some extra plates in to stall them out but I'm not sure for how long.
I honestly expected more contams in this part of the process, so now I've got more plates than I know what to do with. Here are my next few.
This one grew a little oddly. I think it's just the way the myc crawled off the deep wedge corner, but could it maybe be a contam?
And the other 2 coming along nicely.
 
Does that sectoring look like different cultures or just the way it came together? I'd like to figure out how yall get those nice circular punches.
I think I'll make 4 master jars and 2 children from each. My plan was to go G2G 1->9 G1 and 9->81(max) G2 and make bins from those. Now if I end up with 16 master jars am I better off to make bins from G1 instead?
First masters starting off:
 
|
verytastycheese
Tripmaster
Registered: 11/11/14
Posts: 91
Last seen: 20 minutes, 17 seconds
|
|
|
LogicaL Chaos
Ascension Energy | UFOs | 2020
Registered: 05/12/07
Posts: 63,160
Loc: The Inexpressible...
Last seen: 21 hours, 5 minutes
|
|
Awesome pictures! 
|
 TheBoJim
Strangest
Registered: 07/23/20
Posts: 254
Last seen: 3 minutes, 35 seconds
|
|
Quote:
Josex said:
Any size wedge would do. I like little triangular wedges, maybe like twice as big as the wedges you are currently using for agar to agar transfers.
Then, I barely crack open the lid of the grain jar and I use the (sterile) inner rim of the jar to get the wedge off the blade and immediately close the lid again.
It's all done very swiftly, this way you ensure you limit the time you expose the media (grains) to a minimum.
Another advantage is, you can inoculate a lot of jars with one single plate by using small wedges.
You can shake when the wedge grows out about an inch in diameter, then shake again at 30% and maybe a last shake later if needed.
This is very helpful info. Everything you said in this thread in spot on.
Quote:
LogicaL Chaos said:
Awesome pictures!
This should be used as an example of how to take pictures if you want fast and accurate help from others. I shake my head at some of the photos posted here. Its like they never look at the picture before posting. Good job verytastycheese!
|
verytastycheese
Tripmaster
Registered: 11/11/14
Posts: 91
Last seen: 20 minutes, 17 seconds
|
Re: What happened? [Re: TheBoJim] 1
#27114135 - 12/29/20 02:20 PM (25 days, 7 hours ago) |
|
|
Quote:
TheBoJim said:
This should be used as an example of how to take pictures if you want fast and accurate help from others. I shake my head at some of the photos posted here. Its like they never look at the picture before posting. Good job verytastycheese!
Thanks mate! I got a pack of these from Amazon and attached to my shelving and a smart plug to time it all. They're pretty bright but also make for great staging lights. I can understand not everyone has that good of lighting though.
Here are my jars just a few days after their ~30% shake. I figure just a few more days until G2G time.
  
And here is the culture T5 on the new lower nutrient and thinner poured agar. You can clearly see the struggle and stretch for nutrients. It also grew much faster.
 
I now have 16 seemingly clean jars and a bunch of clean plates that I'm not sure what to do with but make more master jars? The closer I stick to the seed the better right? Masters take forever to cultivate so I might go to G1 but I'll be making a shoebox with one of the first jars in the meantime.
Time to make a liquid culture?
|
Professor X
School for the Gifted
Registered: 04/18/19
Posts: 1,005
Last seen: 5 hours, 32 minutes
|
|
Take a flashlight and shine it through the side of the plate then look at it from underneath, that will show you where the contaminants are hiding then transfer away from them.
--------------------
|
verytastycheese
Tripmaster
Registered: 11/11/14
Posts: 91
Last seen: 20 minutes, 17 seconds
|
|
Quote:
Professor X said:
Take a flashlight and shine it through the side of the plate then look at it from underneath, that will show you where the contaminants are hiding then transfer away from them.
Thanks for the advice I'll try that! I'm pretty sure I'm in the clear for now though
|
Josex
Axenic Cheater
Registered: 11/13/15
Posts: 7,207
Loc:
|
|
Shit looking good dude.
|
verytastycheese
Tripmaster
Registered: 11/11/14
Posts: 91
Last seen: 20 minutes, 17 seconds
|
Re: What happened? [Re: Josex] 1
#27129379 - 01/05/21 11:54 PM (17 days, 22 hours ago) |
|
|
Update:
Did my first G2G in front of the LFH. 3 days in they look to have hit 30%+ already so I shook them. Also made a shoebox the same day just to be sure it pins. It looks great so far just waiting on some actual fruits!
|
Shr00merN00ber
Stranger
Registered: 12/19/18
Posts: 232
Last seen: 6 days, 7 hours
|
|
Nice work dude, whats your sub? Coir verm?
|
Justweed
Bobby's short shorts
Registered: 04/01/20
Posts: 355
Last seen: 2 hours, 40 minutes
|
|
Looks great man! Good progress!
--------------------
  
Keep 'em high and tight guys....
|
verytastycheese
Tripmaster
Registered: 11/11/14
Posts: 91
Last seen: 20 minutes, 17 seconds
|
|
Quote:
Shr00merN00ber said:
Nice work dude, whats your sub? Coir verm?
Yep just CVG with a tiny bucket tek. Just 1:1 with one jar for the shoebox. I don't expect much I just want to see pins and no contams.
Edited by verytastycheese (01/06/21 04:45 PM)
|
verytastycheese
Tripmaster
Registered: 11/11/14
Posts: 91
Last seen: 20 minutes, 17 seconds
|
|
I'm on the edge of my seat!
 
|
Professor X
School for the Gifted
Registered: 04/18/19
Posts: 1,005
Last seen: 5 hours, 32 minutes
|
Re: What happened? [Re: Josex]
#27136629 - 01/09/21 01:43 AM (14 days, 20 hours ago) |
|
|
You can use tiny amounts per jar. I use agushy 2oz slime containers from Amazon as plates and do 14 quarts per plate.
--------------------
|
|
Threaded
Previous
Index
Next
Edit 
Reply 
