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OfflineNat Voorhees
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Partially colonized WBS question
    #27078047 - 12/08/20 12:39 AM (3 years, 1 month ago)

I have three WBS quart jars colonizing at the moment, which are supposed to be my spawn for an upcoming grow. Two of them started colonizing right away at about the same rate, the other one took a little longer to get started and continued to colonize slower. I shook all three at the same time because I was going out of town for a few days and didn't want the two faster jars to colonize too much before the shake. The two jars were about 30-40% colonized when I shook them, the slower one was probably less than 10% (I was hoping some of the middle was colonized where I couldn't see). When I came back a few days later, all three seemed to be colonizing healthily, but the one was still not nearly as far along as the others. I gave the slower one a second shake today, seeing if I might be able to get it to speed up and catch up to the others (now almost fully colonized).

So my question is, should I spawn the partially colonized one with the others when they hit 100%, or wait until the last one finishes and lose some of the vigor of the myc in the other two? I realize neither are ideal, just wondering if anyone can lend insight into which course of action would be preferable over the other and why.

Worst case scenario, I'll just scale down this upcoming grow and have a master leftover for some G2G transfers or something.


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My SAB


Edited by Nat Voorhees (12/10/20 09:02 AM)


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Offlineverum subsequentis
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Re: Partially colonized WBS question [Re: Nat Voorhees] * 1
    #27078107 - 12/08/20 02:22 AM (3 years, 1 month ago)

DO not spawn anything uncolonized. Available nutrition (mold food) will be eaten and consumed by the most active and aggressive life form around. Imperfect fungi will take hold, reproduce and completely fuck off everything you've got going.

Either spawn jars that are ready in smaller totes (shoeboxes) or wait for the last one. You aren't going to lose vigor over a couple of days.

PS. posting pics really helps people know what's going on. As it stands I have no idea how far behind or ahead one jar is from another.


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OfflineNat Voorhees
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Re: Partially colonized WBS question [Re: verum subsequentis]
    #27078167 - 12/08/20 04:14 AM (3 years, 1 month ago)

Thank you! I only thought to take a pic after shaking the one, which just looks totally uncolonized now because of the shake. I'll try to remember to document things better in the future.

I'm gonna let them do their thing, if the one takes too long to finish up I will do a smaller shoe box or something, thanks again for the advice! :thumbup:


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Offlineverum subsequentis
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Re: Partially colonized WBS question [Re: Nat Voorhees]
    #27079630 - 12/08/20 10:25 PM (3 years, 1 month ago)

:goodday:


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OfflineNat Voorhees
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Re: Partially colonized WBS question [Re: verum subsequentis]
    #27079895 - 12/09/20 04:36 AM (3 years, 1 month ago)

Here's an update now that the myc has recovered a bit, with one of the other jars for comparison. Think I'm gonna try to just let this last one finish colonizing



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OfflineNat Voorhees
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Re: Partially colonized WBS question [Re: Nat Voorhees]
    #27081808 - 12/10/20 09:01 AM (3 years, 1 month ago)

Yesterday


and just now


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Offlineverum subsequentis
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Re: Partially colonized WBS question [Re: Nat Voorhees]
    #27082047 - 12/10/20 12:01 PM (3 years, 1 month ago)

So you're going to wait and use them all at once?


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OfflineProfessor X
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Re: Partially colonized WBS question [Re: verum subsequentis]
    #27082130 - 12/10/20 12:37 PM (3 years, 1 month ago)

Your 3rd jar may be a bit bacterial. I would spawn the 2 together and it by itself or just run 3 shoeboxes which is the safest route. If that one is bacterial it will cause the rest to teeter ouy prematurely. Sometimes I will spawn jars like that and usually get 1 or 2 flushes before the green comes. Doesn't happen very often anymore though. Good luck with your grow


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My no pour Petri TEK - https://www.shroomery.org/forums/showflat.php/Number/27252059/page/1


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Offlineverum subsequentis
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Re: Partially colonized WBS question [Re: Professor X]
    #27082496 - 12/10/20 03:57 PM (3 years, 1 month ago)

It may be but jars can look that way if you shake late. Unless you're talking about something else. What exactly makes you think it's bacterial?


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OfflineNat Voorhees
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Re: Partially colonized WBS question [Re: verum subsequentis]
    #27084208 - 12/11/20 03:56 PM (3 years, 1 month ago)

Yes I waited and used them all at once, spawned early this morning. The last jar looked like the others by then. I gave them all a good sniff before adding them to my sub and nothing funky, so fingers crossed. I'll post pics soon, but I'll probably start a new thread and just do a whole grow log. Thanks for all the input!


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Offlineverum subsequentis
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Re: Partially colonized WBS question [Re: Nat Voorhees]
    #27084830 - 12/11/20 11:15 PM (3 years, 1 month ago)

I didn't see anything that made me think of bacteria. Looked like it was just catching up from a late shake.

Why not just carry on with this post and do the grow log here?


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OfflineEgohst
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Re: Partially colonized WBS question [Re: verum subsequentis]
    #27084924 - 12/12/20 01:13 AM (3 years, 1 month ago)

When you shook the jar a second time before letting everything reknit you can slow it down. Once I shake I do not touch again most of the time. This reduced contam for me. Some take more time than others. If I’m desperate for that other jar I have just brushed off the uncolonized parts, if that is not possible then DONT SPAWN. Well at least when I spawned uncolonized I got contam. If the jar looked like the others though then it was just a slow starter. Glad to hear the last jar caught up. Don’t introduce to fruiting too early now!


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OfflineNat Voorhees
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Re: Partially colonized WBS question [Re: Egohst]
    #27084951 - 12/12/20 01:59 AM (3 years, 1 month ago)

Just thought posting the whole grow on here might be getting a little off the original topic, but hey if y'all are still here and don't mind why not. I spawned those jars (about 2qt total) to 1 brick of coir with 2qt verm and 1 1/2 cups gypsum. Here's my little 46qt mono


I cut circles out of these polyester filter sheets and put them in those vents


That way I can easily pop the whole vents out when I mist so I don't get the filters all wet, and I figured it's neat and clean and easy to replace, and to make adjustments by adding or taking away layers. I did 1 layer in the top vents and 2 in the bottom for now.

For this grow, I just put it directly into fruiting conditions. I'll definitely try letting some tubs colonize first with future grows, but my tub has one of those weathertight lids and I didn't wanna worry about fussing with dialing in the right amount of GE for colonization. BOD and many others seem to have good luck going directly to fruting, so I'm hoping I'll be okay.


I realize I'm probably experimenting a little too much for my lack of experience, but alas. I'll be back with updates soon


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My SAB


Edited by Nat Voorhees (12/12/20 02:57 PM)


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OfflineNat Voorhees
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Re: Partially colonized WBS question [Re: Nat Voorhees]
    #27085908 - 12/12/20 02:54 PM (3 years, 1 month ago)

Oh, and this was from a MS syringe. Or part of one anyway.

And I realize that spawn ratio is pretty low, that was from 5 dry cups of WBS which I expected to expand a lot more (I pretty much followed Frank's WBS Prep, which says it should expand 3-4x, mine only expanded like 1 1/2x). I'm sure I did something wrong, still not sure what but it seems to have still worked okay. I am a little worried about having used that little spawn though


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Offlineverum subsequentis
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Re: Partially colonized WBS question [Re: Nat Voorhees]
    #27086527 - 12/12/20 11:43 PM (3 years, 1 month ago)

The spawn ratio will be fine if the spawn was clean. You just won't pull as much product out as you would have with more spawn and it'll take a little longer to colonize.

You don't have to dial in conditions if you're just sealing her up to colonized. Anything will work as long as it's not airtight or letting in so much air that it dries out.

Yes, you are experimenting a bit much for a beginner. Just be sure that you aren't drying out too much and watch and learn.


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OfflineNat Voorhees
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Re: Partially colonized WBS question [Re: verum subsequentis]
    #27089721 - 12/15/20 12:06 AM (3 years, 1 month ago)

I really appreciate all the advice and feedback! I've been checking it daily and misted it a couple times to make sure it's not drying out too much. Here's where it's at now


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Offlineverum subsequentis
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Re: Partially colonized WBS question [Re: Nat Voorhees]
    #27089772 - 12/15/20 01:44 AM (3 years, 1 month ago)

Looking good.
Dude, your tub is crazy as fuck. Is that a little light on the side?


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OfflineNat Voorhees
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Re: Partially colonized WBS question [Re: verum subsequentis]
    #27089879 - 12/15/20 05:26 AM (3 years, 1 month ago)

Thank you! and no, just a cheap petco terrarium thermometer. There was a hygrometer too but I was told I probably wouldn't get very accurate readings from it so I gave it away, I wanna be able to eyeball good moisture/humidity anyway


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Edited by Nat Voorhees (07/19/23 11:11 AM)


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Offlinetiptrippy
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Re: Partially colonized WBS question [Re: Nat Voorhees]
    #27090514 - 12/15/20 01:15 PM (3 years, 1 month ago)

Lol you put actual vents in your tub? Are they adjustable or full throttle 100% of the time? Keep an eye on your sub to make sure it doesn't dry out from all that FAE


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OfflineNat Voorhees
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Re: Partially colonized WBS question [Re: tiptrippy]
    #27090619 - 12/15/20 02:17 PM (3 years, 1 month ago)

The vents have polyester discs I cut from these filter sheets in them! Might add more layers as I dial it in


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