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InvisibleTheDoobskerS
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Registered: 05/22/20
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Does this plate succession look normal?
    #27075395 - 12/06/20 02:24 PM (3 years, 1 month ago)

Hoping I can get some advice and/or confirmation that I'm doing this right...


I had this BM T2 plate that I put to a few oats jars and made transfers from



And here are the transfer plates, which I expected to look better. To me, none of them are even on par with the donor plate which confuses me.




All of these T3 blue plates are 20g agar/14g LME per 1000ml
All of the non-colored plates are 20g agar/16g LME per 1000ml.
I lowered the LME thinking maybe I'd get some good rhizo growth.


And here are my tamp T2-->T3 xfers and some rye jars I put T2 plates to. I suppose it's too soon to tell, but to me the growth looks weaker than the T2 plates did at this size





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InvisibleTheDoobskerS
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Re: Does this plate succession look normal? [Re: TheDoobsker]
    #27075920 - 12/06/20 06:57 PM (3 years, 1 month ago)

Bump


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Offlinebw86
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Registered: 11/12/06
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Re: Does this plate succession look normal? [Re: TheDoobsker]
    #27075929 - 12/06/20 07:00 PM (3 years, 1 month ago)

Don't bump your own thread within a 24 hour period.
Everything looks fine.


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OfflineA.k.aM
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Re: Does this plate succession look normal? [Re: bw86] * 1
    #27075996 - 12/06/20 07:26 PM (3 years, 1 month ago)

Try doing it without the coloring.


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LAGM2020


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OfflineJustweed
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Registered: 04/01/20
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Re: Does this plate succession look normal? [Re: A.k.a]
    #27076109 - 12/06/20 08:30 PM (3 years, 1 month ago)

They look a bit wispy but that could be from conditions. Try to keep steady humidity and temperature in the environment. The original plates weren't exactly super ropey. I'm not an agar expert, but I think if you keep doing transfers you might be able to isolate a more rhizo section, but if I were you I'd grow some out on grain, spawn it, and start again either from spores or from a clone.


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Keep 'em high and tight guys....


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InvisibleTheDoobskerS
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Re: Does this plate succession look normal? [Re: Justweed]
    #27076129 - 12/06/20 08:41 PM (3 years, 1 month ago)

Quote:

bw86 said:
Don't bump your own thread within a 24 hour period.




Got it :thumbup:
My bad.

Quote:

A.k.a said:
Try doing it without the coloring.



Poured a bunch of plates yesterday with 14g LME and left out the coloring. Although, I have compared plates with/without this food coloring in the past and noticed no difference.

Quote:

Justweed said:
They look a bit wispy but that could be from conditions. Try to keep steady humidity and temperature in the environment. The original plates weren't exactly super ropey. I'm not an agar expert, but I think if you keep doing transfers you might be able to isolate a more rhizo section, but if I were you I'd grow some out on grain, spawn it, and start again either from spores or from a clone.



I'd imagine humidity stays fairly consistent in my house. Temp definitely fluctuates a fair amount. I suppose I could try to stabilize that better.

Maybe I should just keep the succession going. I’ve trashed a lot of cultures simply because they weren’t getting better with each transfer. I think the highest I’ve been up to is T7, and honestly that culture was trash from the beginning.
Maybe I just need to eat more shrooms and be more patient.
Appreciate the feedback guys :thumbup:


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