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Offlinetroy_white42
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Greetings seasoned micologists! * 1
    #27071652 - 12/04/20 11:30 AM (3 years, 1 month ago)

Hello! First id like to start about how stoked I am to finally do this. Been an avid mushroom hunter since a kid starting with morals and peckerheads.  Recently decided to branch off my foraging and study species from a book.  Took a trip to the smokies and found a lot of new stuff including a couple lbs of wild lion mane!  First time eating and iding it! YUM!

Been doing a lot of research about cultivation and decided to convert a spare room into a grow room with individual mini greenhouse shelves and a fog pvc system for air exchange and humidity.  Ordered a bunch of base material to start practicing my agar dishes and LC.  Ordered about 10 different strains of syringe oysters, hen, chicken, and lions mane to get started.  Id like to build up a library of clones and spores to ensure I've got plenty of back ups as I'm sure ill have plenty of oppsies learning practically.  Fully intend of sharing pictures of the process and grows to get and share ideas.

Anyone got some spore prints they'd be willing to share that would be fun to grow and add to by base collection?  I've been reading the forum for a few weeks now and decided why the hell not?  Time to jump in!


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OfflineSHROOMSISAY01M
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Re: Greetings seasoned micologists! [Re: troy_white42]
    #27071793 - 12/04/20 12:43 PM (3 years, 1 month ago)

You need to exhaust air out and not just draw air in. I am not sure what you mean by a fog PVC system for air exchange and humidity. Any way you want to build a passive exhaust system especially since it sounds like you will be growing close to where you live. A passive system will draw air in as it exhausts air and spores out...



The flapper will not work unless you have a sealed tent or room. If it is not sealed it will just draw air in where ever it can get air and the flapper will not open.

https://www.amazon.com/Connector-Plastic-Straight-Heating-Ventilation/dp/B0822TDWNF/ref=sr_1_91?dchild=1&keywords=air+duct+damper&qid=1607111100&sr=8-91


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Offlinetroy_white42
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Re: Greetings seasoned micologists! [Re: SHROOMSISAY01]
    #27071913 - 12/04/20 01:32 PM (3 years, 1 month ago)

Essentially two 3 tier racks with each row split into different separate tanks to minimalize cross contamination of specimen with 3mm tarp.  A 2" pvc pipe runs through each tank with holes drilled in each tank for air/humidity feeding from a Tupperware water box with a disc fogger and fan to push psotive pressure. 

Still working on the idea of venting as its set up in my spare bedroom.  Can't really do negative pressure since I'm using my central air to control temp. I can open the window and vent the room manually or I've flirted with installing filters on each tank to push out the positive airflow from the pvc into the room and filter the spores.  I'm still framing the woodwork atm got about 2 weeks for my supplies to start growing comes in.  Totally educated trial by fire guessing here lol.  Input welcome


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OfflineSHROOMSISAY01M
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Re: Greetings seasoned micologists! [Re: troy_white42]
    #27071987 - 12/04/20 02:16 PM (3 years, 1 month ago)

Get a piece of plywood cut a hole in it for exhaust and stick it in a window You can use rigid foam insulation to insulate if you need to...

https://www.homedepot.com/s/rmax%2520rigid%2520foam%2520insulation%2520board?NCNI-5

without exhausting the co2 there won't be many mushrooms you can grow especially oysters and LM.


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OfflineSHROOMSISAY01M
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Re: Greetings seasoned micologists! [Re: SHROOMSISAY01]
    #27071998 - 12/04/20 02:21 PM (3 years, 1 month ago)

I think you are overthinking this as long as the growing parameters are the same you can grow different species in the same tent.


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OfflineSHROOMSISAY01M
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Re: Greetings seasoned micologists! [Re: SHROOMSISAY01]
    #27072013 - 12/04/20 02:28 PM (3 years, 1 month ago)

I am assuming you are going to put a mini greenhouse in the room.


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Offlinetroy_white42
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Re: Greetings seasoned micologists! [Re: SHROOMSISAY01]
    #27073034 - 12/05/20 07:21 AM (3 years, 1 month ago)

Thats my super power. Overthinking lol.  So I think I'll have to go with the vent for the window but I'm going to test some negative pressure with .02 micron filters.  Yes essentially indoor greenhouse. Current framing phase.  Stoked! Trying to figure out pic uploads from the phone but ill post em when I get it figured out.


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OfflineSHROOMSISAY01M
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Re: Greetings seasoned micologists! [Re: troy_white42]
    #27073291 - 12/05/20 10:39 AM (3 years, 1 month ago)

If you want to test with a filter that is your choice but you will soon find out it is a waste of money. Filters stop up quickly and must be replaced frequently. It will get very expensive with little benifit.


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OfflineSHROOMSISAY01M
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Re: Greetings seasoned micologists! [Re: SHROOMSISAY01] * 1
    #27073308 - 12/05/20 10:46 AM (3 years, 1 month ago)

If you use a negative pressure there is really no need for a filter the exhaust vent will take a lot of the spores out of the room. I would still use a mask rated for spores while in the room/tent. I know that is the last thing you want to hear right now. Someone telling you to wear a mask. At least I am not affiliated with the government.


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Offlinetroy_white42
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Re: Greetings seasoned micologists! [Re: SHROOMSISAY01]
    #27075104 - 12/06/20 11:28 AM (3 years, 1 month ago)

I've done the plywood window trick before for construction so I'll look into it after I get my initial setup a test run.  I've got a few initial concerns floating in my head bit I need to finish building and test the power of the disc fogger and fan to see how much it will push through a 4 tiered greenhouse shelf (5'x8'x2').  The airflow will be coming from a big Tupperware box filled with water and a disc from hoh.  A circular fan will push the air/fog from the box via dryer duct into a 2"pvc into the greenhouse.  I'm wondering if I should start the pvc on top or bottom (will the fog/humidity rise or fall ).  Will that be enough FAE set on a humidity controller?  Should I install a secondary exhaust port for + pressure and a fan on one side with a - pressure fan porting to the window exhaust?  Will that be too much FAE and/or kill my humidity if I set it on an independent timer?  My lighting is from a western facing bedroom window and I have a few LED 6500k strips if I need more.

Other thoughts:
Do you inoculate the substrate bag independent with a LC syringe or do you inoculate the wbf/GS jars first and dump them into the substrate after colonization?

What can I use for substrate bag gas exchange ports?  I ordered some cut to fit filter sheets but I don't think they're 99.9% like the one I ordered for my lamar hood build.  Links!

Do you need different agar recipes for different species LC grows?


Edited by troy_white42 (12/06/20 11:44 AM)


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OfflineSHROOMSISAY01M
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Re: Greetings seasoned micologists! [Re: troy_white42]
    #27076106 - 12/06/20 08:28 PM (3 years, 1 month ago)

Humidity will rise. Where do the clouds form? In the sky because humidity rises.

I do not know what size fan you have to push the humidity but realize you will lose a lot of the force of the fan going through a flexible duct with a wire in it. The air hits the wire every few inches and slows down. Also, realize you are putting humidity through whatever duct you decide to use and water will build up in the duct if it is not slanted toward where the humidity is coming from. If you slant it. It will run back into your bucket. Unless it is a duct that has the wire in it. If it has a wire it will be traped. Best to use 4" PVC for your ducting.


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Offlinetroy_white42
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Re: Greetings seasoned micologists! [Re: SHROOMSISAY01]
    #27076121 - 12/06/20 08:36 PM (3 years, 1 month ago)

Interesting concepts I've been flirting but trying to be cheap.  I've already dumped $700+ which single bad for my grow size from scratch.  I'm pretty sure you're right about the fan size for the + pressure which is pushing the fogger.  I'm only curious if the- pressure for the out fan will compensate its loss.  I'm gonna test a couple dry runs soon. 

I thought about the clouds but also the temp fluxs in natural environments.  Hot days cold nights.  Fog generally stays low then rises with heat.  My grow is in a 70°- perm environment.  Either way I can start up top with a shelf with + pressure and suck it out though the outtake for FAE with - pressure ensuring they all get a taste of the humidity  or vice versa.  From the bottom the splash back will be in the original water box but up top will prob be the greenhouse.  Disnt think of that.  Migbt upgrade my + fan depending on test runs.


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OfflineSHROOMSISAY01M
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Re: Greetings seasoned micologists! [Re: troy_white42]
    #27076248 - 12/06/20 10:09 PM (3 years, 1 month ago)

I don't know if I have told you but you really need a circulation fan inside your room just moving the air around but not blowing on the mushrooms and if you are using a humidity controller place the probe on the intake of the fan. I use a painter's booth filter so the spores don't damage the prob when I change the filter I wash out the old one and use it again the next time.


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Offlinetroy_white42
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Re: Greetings seasoned micologists! [Re: SHROOMSISAY01]
    #27076633 - 12/07/20 06:45 AM (3 years, 1 month ago)

I think the +/- in out fans will suffice exchange with my set up.  Didnt think of the probe filter thanks.  Here's my set up thusfar after framing.  Still a lot of work to do.  Air will blow in the top on the red line and get sucked out at the bottom following each enclosed level with a vent hole at each levels opposing end to ensure equal humidity spread.  I'll post more as I get progress. Side note: I'm starting to doubt my single disc fogger will produce enough for the cubic foot of this enclosure.  I'm hoping since its air tight it will man up to the jon just have to run longer.  May need to upgrade to a 4 disc but ill test run.  Also the + pressure fan may need a boost but I can prob swap out my lamar hoods floor blower and just pull it when I need it for the lamar work.  So much fun problem solving and my cultures haven't even arrived. 🤣

https://ibb.co/tM8szV8


Edited by troy_white42 (12/07/20 08:16 AM)


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OfflineSHROOMSISAY01M
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Re: Greetings seasoned micologists! [Re: troy_white42]
    #27077258 - 12/07/20 02:31 PM (3 years, 1 month ago)

My point is that for a small amount more you will have a perfect system rather than drilling holes in the pipe and having humidity come out in different places you buy a 3 or 4-inch bilge fan  and where ever your humidity is coming in just blow the air around. You will notice if you are growing oysters especially that you will get long stipes without a circulation fan. Co2 builds up around the mushrooms quickly without a circulation fan. A water-resistant bilge fan is cheap.

4 inch...

https://www.amazon.com/Attwood-1749-4-Blower-Resistant-4-Inch/dp/B003EX02DA/ref=sr_1_23_sspa?dchild=1&keywords=bilge+fan&qid=1607375944&sr=8-23-spons&psc=1&spLa=ZW5jcnlwdGVkUXVhbGlmaWVyPUEyRDFTU1FDWVlWRkI3JmVuY3J5cHRlZElkPUEwMDY3MTUxMlM1M1U2V1hTMDBMWCZlbmNyeXB0ZWRBZElkPUEwNDk2MTMyMzlVU1pJUUZOSVlIUyZ3aWRnZXROYW1lPXNwX210ZiZhY3Rpb249Y2xpY2tSZWRpcmVjdCZkb05vdExvZ0NsaWNrPXRydWU=

3 inch...

https://www.amazon.com/attwood-Blower-Resist-White-3-Inch/dp/B001O0DE9E/ref=sr_1_5?dchild=1&keywords=3%22+bilge+fan&qid=1607376080&sr=8-5

I personally would use the 4 " and a https://www.amazon.com/attwood-Blower-Resist-White-3-Inch/dp/B001O0DE9E/ref=sr_1_5?dchild=1&keywords=3%22+bilge+fan&qid=1607376080&sr=8-5

I personally would use the 4 in with a 12 volt Speed Controller to control the speed of the fan. But you would need at least a 5 amp speed controller.

Anyway, I will not beat a dead horse. If you don't want to use a circulation fan it is your choice but you will eventually find out you need one.


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OfflinePossum-Pie
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Re: Greetings seasoned micologists! [Re: SHROOMSISAY01]
    #27078170 - 12/08/20 04:23 AM (3 years, 1 month ago)

Hi, I'm relatively new to the forum and a healthcare provider. I've seen folks laugh off spore inhalation and folks overly paranoid about it. Human fungal infections are almost all topical-athlete's foot, yeast infections, toenail infections, etc.  Annoyances. HOWEVER severely immunocompromised people can get internal fungal infections which are very dangerous.  I'd say casual contact with inhaled spores in a person with an intact immune system is perfectly safe. Hours/day of close contact such as a commercial grow room needs a filter mask. Sounds like your hobby room should be safe to enter without a mask however beware that being inside a residence infants, elderly, people with HIV, or other immunocompromised people probably should not go in.

This information is meant to be instructional only. Any questions about your individual situation should be directed to your personal physician.


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OfflineSHROOMSISAY01M
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Re: Greetings seasoned micologists! [Re: Possum-Pie]
    #27078356 - 12/08/20 08:15 AM (3 years, 1 month ago)

From what I hear when you breathe in a spore it is always there. That spore will never leave your lungs. If that is true an accumulative effect would be bad for anyone. I also don't think you realize how bad the spore load can be. If I have a spore drop my whole room and everything in it will be covered with white dust. Be careful everyone your health is nothing to play with. I would rather put on a mask and not need it than to have needed it and not wear it!! There was just a post a few days ago that talked about flu-like symptoms from spore inhalation.


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Re: Greetings seasoned micologists! [Re: SHROOMSISAY01]
    #27078378 - 12/08/20 08:28 AM (3 years, 1 month ago)

Inhalation of any particulate such as smoke, dust, pollen, etc. can cause a reaction if a high enough concentration and a long enough period of time. Most mushroom spore reactions are actually not from an "infection", but from an autoimmune mediated allergic reaction called Hypersensitivity Pneumonitis Induced by Spores.  As I mentioned previously, casual inhalation is no more dangerous than occasionally sitting around a campfire compared to someone smoking filter-less cigarettes for 40 years.  It's a matter of how much and how long. There are many documented cases of Hypersensitivity Pneumonitis Induced by Shiitake mushrooms. If it makes you feel better to mask up for a home grow room, it certainly won't hurt.


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OfflineSHROOMSISAY01M
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Re: Greetings seasoned micologists! [Re: Possum-Pie]
    #27078883 - 12/08/20 02:49 PM (3 years, 1 month ago)

Good info. Thanks for your input. We appreciate it!!


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Offlinetaku
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Re: Greetings seasoned micologists! [Re: SHROOMSISAY01]
    #27079395 - 12/08/20 07:54 PM (3 years, 1 month ago)

In terms of the circulation fan, would it be enough to have a fan blowing in fresh air with the fog in tandem with a humidity controller and an exhaust fan set to run 6 times an hour? Does that circulate the air enough? Or have a small fan inside running at all times?


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OfflineSHROOMSISAY01M
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Re: Greetings seasoned micologists! [Re: taku]
    #27079580 - 12/08/20 09:50 PM (3 years, 1 month ago)

Yea you want a fan inside running all the time but not blowing on the mushrooms. That little bit of fan blowing the humidity in is not enough.


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Re: Greetings seasoned micologists! [Re: SHROOMSISAY01]
    #27087904 - 12/13/20 08:30 PM (3 years, 1 month ago)

Amazon and an entire weekend later I've got some major first timer status going.  Hoping all goes well but I'm sure I'll run into some hurdles.  So about 30 bags sealed, mixed, filtered, and gaskets.  All PC for an hour and knocked up with about 2cc of LC from a vendor.  Kncoked up 2 half pints each of wbs and homemade LC mix with 1cc of the same syringe that I also PC for backup.  13 species mostly oyster variants and LM/BH.

Got about 1cc left in each syringe that I'm saving to knock up some baby food jars for agar.  Gonna PC them with ports and filters.    Keeping it all in my spare room at about 70-75°.  Gonna work on my fruiting chamber while they do there thing but I'm excited. What am I forgetting?

https://ibb.co/GF5kqyn

https://ibb.co/4KF7rsC

Oh, I've got a 99.9 12×12 filter that I'm going to build a lamar hood too.  Just wanted to kncok the bags up.  Timing issue with amazon.. I will do everything lamar next go around.  Hoping the contam isn't too bad firing the needles.


Edited by troy_white42 (12/13/20 08:33 PM)


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Offlinetroy_white42
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Re: Greetings seasoned micologists! [Re: troy_white42]
    #27090968 - 12/15/20 06:16 PM (3 years, 1 month ago)

So day 3 after injections to 30 5lb bags (5c hwfp/1.5c Rice bran/1c wbs/500ml water), 20 grain half pints (wbs), 20 half pints LC (5ml water/5grams LME/1tsp karo), 15 baby food jars agar (500ml water/5g LME/1tsp karo/agar).

Seeing whisp starters on everything from splitting a 10CC vendor syringe up 1 each with 13 species. Bh and LM none yet. Spread it thin to clone.  Forgot to take pics before I shook. Doh.  BO and FO seem to be the fastest sprouted with BO out front by margin.  LCs seem to be growing in size and clear clumped colonies minis the degrees that o had problems with PCing.

Think I figured out the degree problem.  Karo was thickening on the bottom of the pan.  I poured it cold.  Double coffee filtered the new LC mix for the gallon jars that I'm going to put the agar growths into once they finish out and I get my lamar built.  Excited to see baby progress

Decided to polish off some Jameson and build the box.it ain't pretty but it's air tight.  Got a floor fan and filter to spec the cubic foot and 99.9%.  Waiting on the silicon to dry to give er a test run.

https://ibb.co/zh7GSTy




Edited by troy_white42 (12/15/20 08:15 PM)


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Re: Greetings seasoned micologists! [Re: troy_white42]
    #27091146 - 12/15/20 08:34 PM (3 years, 1 month ago)

you should nickname that lammy "The Hammerfist" - definitely has a jameson-build vibe to it :grin:

best of luck with your colonizing!


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Re: Greetings seasoned micologists! [Re: troy_white42]
    #27100497 - 12/21/20 08:38 AM (3 years, 1 month ago)

So my original bags have about a golf ball size growth that I've shook up.  My agar have bloomed nicely and look clean except the chicken.  It seems so be the slowest in par with the lion mane.  Is that normal?  Also, my black moral agar looks clean but the underlayer is green/black.  I'm wondering if that's just normal it's super aggressive.  Can't find any images of the mycelium to compare.

I've cloned the agar onto slants and made fresh LC jars from it.  Waitibg to see how those turn out.  Lost a shitake and maitakke jar to contam nut not bad 2/30 jars.  Set them in a separate closet to study whT happens.  They got all cloudy. 

Knocked up another 30 bags and installed LEDs and shelves into my colonizing closet so I've got about 70 bags in there atm. The grain spawn jars are all looking nice and fuzzy.  Lm and BH super slow like everything else.

So I've noticed the the bags self heating and learned about thermogenesis.  Where the bags touch they conduct heat exchange and get in the upper 90s.  Playing with temperature control but wondering if I should not let the bags touch.  75-85 soil temp where they aren't touching depending on how high up in the closet they are. I've got my lower temp species in the bottom where it's cooler and and higher temp like Florida oyster and gulf oyster up top.

Also have a lot of air in the bags than when I starte.  Guess this is from the filter patch and co2 build up.  Worried it might start pints or is that fine since it's prob all co2?  Better pics to come.  Just been slammed with initial build and cloning.

https://ibb.co/mG5wkwm
https://ibb.co/bdNPd8H


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OfflineSHROOMSISAY01M
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Re: Greetings seasoned micologists! [Re: troy_white42]
    #27101136 - 12/21/20 03:42 PM (3 years, 1 month ago)

LM is very thin look again closely you are probably missing it. It does not look like any other mycelium until it has set for a long time then it starts to thicken up.


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Re: Greetings seasoned micologists! [Re: SHROOMSISAY01]
    #27110688 - 12/27/20 01:14 PM (3 years, 1 month ago)

So massive production underway.  Decided to start converting a second closet to colonize bags since the first is full.  My initial bags are starting to colonize.  Some slower than others but the pinks, Florida's and elms are taking off.  The shitake, mistake, chickens, and manes are slow but showing growth. Does the last bag look too dry?  I was thinking about injecting like 100cc of my clean LC broth to rehydrate?

https://ibb.co/GVnwJ31
https://ibb.co/VBsL0S8
https://ibb.co/7YKFY3S
https://ibb.co/0h0Chxv


Converted a half closet for my agar, and LC mixes.  Lost about 3 jars from my original batches but learned a lot of sterile tek since and ordered some 32oz jars to xfer the agar to lc since my first batch was just LC to LC.  Also learned how to get the damn sediment out of my LC.  It was either the LME or karo after PCing.  Double coffee filtered it after and recoooked for a clean LC. Most of the manes seem to not want to spread across the agar and just bubble up.  I'm not sure if it's forcing fruiting or just mycelium?

https://ibb.co/NSv40BX
https://ibb.co/tCfMDgZ
https://ibb.co/s3p43zK
https://ibb.co/JRtYpcS
https://ibb.co/RYwH2Bd

The wbs jars are all doing well and some looking like fully colonized.  I don't think I trust my skill to G2G my bags yet so I'll stick to LC injections with sealed bags.  Not quite understanding how to keep it sterile with just folding a tyvek sleeve.  Might practice when I catch up.  This jar of BH looks like it's fruiting or is that just mycelium?

https://ibb.co/JrgKkYN

Working on my fruiting chamber om my cloning and PCing breaks.  Its coming along.  Almost ready to test run the humidity fogger tub and see what I can regulate with FAE fans.  More to come.

https://ibb.co/j6XMmpF


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Re: Greetings seasoned micologists! [Re: troy_white42]
    #27124565 - 01/03/21 07:17 PM (3 years, 24 days ago)

So learned a lot about proper bag seals.  I was mixing my hwfp/bran in the bags and then sealing. That left micro holes from stretching the plastic when sealing.  Noticing contam in a lot of bags.  Sucks since I made about 100 that way.  Oh well!  Made another 50 with proper seals.  Also, note to self wbs germinates.. use it before you lose it.


Learning agar. It's a pain but it's legit for identifying if your batches are bad and isolating.  Need a bigger hood.  Now that I've got my jars built up I've graduated to 32oz jars with drilled injection ports and filters not just jb weld glued filters and gasket maker.  Blew a few on first PC and learned to seal both sides of the lids.

My first grain jars finished and I tested them on substrate bags.  Totally exploded.  Worth the time.  Got 5lb bags of wbs cooking atm to knock up for g2g xfer on the next go.  Wondering how to get around the xfer from pressure cooker to.flow hood for contam since it'll only have a tyvec sleeve.


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Re: Greetings seasoned micologists! [Re: troy_white42]
    #27124692 - 01/03/21 08:26 PM (3 years, 24 days ago)

No Tyvek sleeve is needed just fold the bags properly they will be sealed so no need to worry about moving them from the PC to the hood...

&ab_channel=ChuckTaylor


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Re: Greetings seasoned micologists! [Re: troy_white42]
    #27125210 - 01/04/21 04:17 AM (3 years, 24 days ago)

Quote:

troy_white42 said:
So learned a lot about proper bag seals.  I was mixing my hwfp/bran in the bags and then sealing. That left micro holes from stretching the plastic when sealing.  Noticing contam in a lot of bags.  Sucks since I made about 100 that way.  Oh well!  Made another 50 with proper seals.  Also, note to self wbs germinates.. use it before you lose it.


Learning agar. It's a pain but it's legit for identifying if your batches are bad and isolating.  Need a bigger hood.  Now that I've got my jars built up I've graduated to 32oz jars with drilled injection ports and filters not just jb weld glued filters and gasket maker.  Blew a few on first PC and learned to seal both sides of the lids.

My first grain jars finished and I tested them on substrate bags.  Totally exploded.  Worth the time.  Got 5lb bags of wbs cooking atm to knock up for g2g xfer on the next go.  Wondering how to get around the xfer from pressure cooker to.flow hood for contam since it'll only have a tyvec sleeve.




I've been following your progress with your new hobby on this thread. I have to admire you jumping in with both feet right off the bat. I too am relatively new to mycology, but I have started in a timid manner, 3 bags of each species, writing everything I do and the dates in a journal in case I need to find out where I failed. 
A few observations: YES If you have that many bags in a relatively small space  you definitely need to separate them for airflow as exothermic reactions will make them hot.  My 3 bags in a large room give negligible heat but the number you have will get hot. I read about sealing your bags and it seems you are doing it too soon. I mix, fold down the tops (Never used Tyvek they don't stick) and stack them unsealed into the PC. After they cool overnight with the lid still on, I take them out one at a time, inoculate them, heat seal the tops and shelve them. YES, I too have had the heat sealer melt small holes in the Unicorn bags once, but I'm unlatching the heat sealer a few seconds before the light goes off and have stopped having holes.  Never yet had contamination. As for the "Air" in the bags, it is a mixture of room air, with added CO2 as the mycelium respirates. I made a huge mistake the first batch of Shiitake I did by over-filling the bags. CO2 couldn't transpire fast enough with little head-space in bags and it died.
I'll be watching your progress!! I have my first harvest this week of Maitake which has been a slow process but has actually worked!  Now I am getting ready to re-try Shiitake after every bag failed to fruit the first attempt I made.


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Re: Greetings seasoned micologists! [Re: Possum-Pie]
    #27126031 - 01/04/21 12:07 PM (3 years, 24 days ago)

The fold and dash from the kitchen pc to the lab bedroom makes me nervous lol.  I guess I'll give it a go when these new 5lb wbs colonize for g2g xfers.  Injectioning 10 of them today with different species.  Plan on using those to knock up future bags rather than LC injections.  Only gonna use LC when I'm out of grain and need bags.

Ya, I figured the bigger production would give me a bigger test groups to learn from.  I've definitely learned a lot in this month of growing so thanks!  I'm hoping to start fruiting a few BO and PO next week fingers crossed. Will get a chance to test my fruiting chamber in action which btw I switched from a 1 disc to a 6 disc fogger.  It should be here this week.  Good luck possum! More pics to come


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Re: Greetings seasoned micologists! [Re: troy_white42]
    #27127356 - 01/05/21 01:54 AM (3 years, 23 days ago)

wait I am confused you are fruiting pink oyster and blue oyster in the same room? Pink is warm weather and blue is cold weather.


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Re: Greetings seasoned micologists! [Re: SHROOMSISAY01]
    #27127570 - 01/05/21 05:58 AM (3 years, 23 days ago)

I've got 13 different species colonizing.  Going to test run all of them.  Closing some vents off and stuff for different temp zones.


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Re: Greetings seasoned micologists! [Re: troy_white42]
    #27128648 - 01/05/21 04:16 PM (3 years, 23 days ago)

Ok, I understand. I just grow what is in season that way no controlling temps.


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Re: Greetings seasoned micologists! [Re: SHROOMSISAY01]
    #27129831 - 01/06/21 07:31 AM (3 years, 22 days ago)

Alrighty, got my new 32oz jars in and cooked up 24 jars of LC using 5g LME 1tsp karo per 500ml water. The next batch I'm swapping corn dextrose for karo after some research.  2 jars per species knocked up with a master agar that I grew from the original syringe.  They're looking good after just a couple days.  Here's the LM

https://ibb.co/kc5VMmd

Starting my slurry of agar plates now to test each jar for contams.  Dropping a drip of LC to 3 agar plates for each jar so that's 36 plates.  Fml.  Side note: the condensation on the lids really piss me off and I'm trying to find work around to remove it without having to wait for the agar to cool to almost gel state prior to pour.

https://ibb.co/2YRJWxW

Pulled a test bag of black morel that's colonized.  I zip tied below the filter cut some slits and throwing it in the fridge for a couple weeks to see what happens.  I heard the nay sayers but that just made me want to try more. 

https://ibb.co/pPM6QTy

Also cooked up 13 5lb bags of WBS and knocked em up with LC to start my G2G for next round of bags.  Liked the growth rate explosions and I figure it can't hurt to get a round of grain going while these bags are doing there thing.  Need to finish the agar, monitor them, and verify clean LC jars then I should have 180+ syringe pulls per species!  I'll probably grow the agar in my smaller half pints jars after to fruit and verify species.  Considering scrapping the self contained shelf green houses and just converting the room into a greenhouse tent.  Wondering if the 6 disc fogger will work for a 15×15 room.


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Re: Greetings seasoned micologists! [Re: troy_white42]
    #27134740 - 01/08/21 08:48 AM (3 years, 20 days ago)

Scraped my 10g tupperware box and found a cheap plastic plastic 55g drum from a local car wash.  Mounted the inline fan cut a water hole and installed a door/seal on it to put in the water and fit the 6 disc fogger inside.  JB quick welded a pvc joint onto the lid so I can run my pvc out.  Works pretty well on an initial test run!  I'm stoked to get it piped to the tent and see what it can do.

  All my new jars are starting to colonize well but I'm pretty sure my agar had contam on my chicken LS cuz they went all murky.  Set them aside to assess a few days.  Gonna throw my original bags in the fruiting chamber in the next couple days even though they had poor seals and possible contams just to see what they do.

https://ibb.co/CKj7phM


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Re: Greetings seasoned micologists! [Re: troy_white42]
    #27137528 - 01/09/21 11:55 AM (3 years, 19 days ago)

Scrapping the shelf tent idea and using 2x4x8s the frame 10x8x9 tent in the room.  Its just less work and more sq ft for a fruiting chamber.  The shelve is just a pain in the ass to open and close.  Using 3mm 25x10 plastic sheeting to make the tent walls and going to gorilla glue a velcro door to access.  Converted a 55g drum ($25 from a carwash) into a humidifier with a 6 disc fogger and an inline fan with an ink bird to control RH.

https://ibb.co/C7LTgBb

Finished the window exhaust out with a flapper and inline fan to push - pressure out the window with spores.  Going to install a + pressure inline with flapper on the other side both set to run on a cycle timer every 15m for FAE.  Going to let my room ceiling fan do the work inside the tent for pushing around air and humidity.  Not really concerned if it gets fudged in time from spores and/or humidity.  The window with shades tilted inverted will do the work for light.  Going to install 2x4x8 shelves with probably pvc or 1x1x8 racks to hold the bags.

https://ibb.co/553XKdJ

Since the shelf tent is already built I got it hooked up and running on 2 tiers at 97% humidity.  I threw in a dozen of my most promising bags from my first trial run making bags.  Some look decent.  Some not so decent but I figure why not.  Very least cam get a few fruits and do some LT injects into my 32oz jars later so I know I got a 100% clean LC next go around.  My current LC jars i feel confident but I am still figuring out agar so it's hard to be sure. 

Oh and neat idea.  I got a bunch of whiskey jars sitting around so I tyvek zipped the tops and loaded them with HWFP and bran.  Cooked em.up and put gaskets on them for a LC injection.  Figured it would be a fun gift or just a cool grow on the counter.

https://ibb.co/wSQYKQp


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Re: Greetings seasoned micologists! [Re: troy_white42]
    #27137553 - 01/09/21 12:11 PM (3 years, 19 days ago)

How are you gonna clean your humidifier? its pulling in room air so spores and dust etc, will be sitting in water for a while. it gets weird. not to mention bugs flying in and dying in there.


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Re: Greetings seasoned micologists! [Re: deadmandave]
    #27137587 - 01/09/21 12:34 PM (3 years, 19 days ago)

You, sir, are a do-er


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Re: Greetings seasoned micologists! [Re: taku]
    #27137683 - 01/09/21 01:29 PM (3 years, 19 days ago)

Haha thanks take.  I like Projects.  The humidifier will be outside the tent piping in air from outside.  I was thinking peroxide mix in the water and dragging it outside on occasion and hosing it out.


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Re: Greetings seasoned micologists! [Re: troy_white42]
    #27138133 - 01/09/21 05:43 PM (3 years, 19 days ago)

Antibiotics research and testing.  I saved my couple bad LC jars to test and play so no loss if I mess them up.  I'm curious about Antibiotics and if we can clean up LC oppsies.  What can you use thats available over the counter?  Anyone tested this?  Found a few posts but nothing with results.

https://www.chewy.com/fish-aid-antibiotics-amoxicillin/dp/185202?utm_source=google-product&utm_medium=cpc&utm_campaign=hg&utm_content=Fish%20Aid%20Antibiotics&utm_term=&gclid=CjwKCAiAxeX_BRASEiwAc1QdkQshQMvVlJKHCyY1muB-O9gwKnhSQDahULkovu5-kWCOZzpGYMlCNRoCUnEQAvD_BwE


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Re: Greetings seasoned micologists! [Re: troy_white42]
    #27138180 - 01/09/21 06:18 PM (3 years, 19 days ago)

From a medical perspective, you really need to know the genus of bacteria. Gram Negative only are killed by certain antibiotics while gram positive respond to penicillin family like amoxicillin or Augmentin. I don't really think antibiotic treatment would work.  Anaerobic bacteria may respond to hydrogen peroxide spray, but I'm thinking that once contaminated, it's best to isolate and discard without allowing it to spread to unaffected mycelium.  It's not even certain that the contaminate is a bacteria...it may be another fungus. fungicides such as miconazole may kill the desired fungi along with the contaminant. In theory, you certainly could culture and sensitize the contaminant to find the appropriate antibiotic, but it's too much trouble and too expensive without the proper microscope, agar plates, and knowledge base.  The Amox. you linked to would really only be effective against Staph. aureus and a few other common human pathogens. When a doctor prescribes an antibiotic, it usually is guess work depending on where the infection is...Staph and Strep being the most common.


Edited by Possum-Pie (01/09/21 06:26 PM)


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Re: Greetings seasoned micologists! [Re: Possum-Pie]
    #27138202 - 01/09/21 06:28 PM (3 years, 19 days ago)

Ya, I get the hassle factor to really do the science of isolate the bacteria and grow it to treat it.  Not worth the time or money when you could just cook up another batch and start over.  I figure what the hell tho for a $12 bottle I could do some research on jars I don't trust and try to agar them after tonsee if it did anything. Was just curious if anyone's tried it.


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Re: Greetings seasoned micologists! [Re: troy_white42]
    #27138256 - 01/09/21 06:55 PM (3 years, 19 days ago)

Better off doing grain and then just G2G from there. LC is too unreliable but you will learn that if you keep going down this path.


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Re: Greetings seasoned micologists! [Re: SHROOMSISAY01]
    #27138482 - 01/09/21 08:52 PM (3 years, 18 days ago)

LT to LC seems almost fail proof.  Then LC to grain.  What you use to inocnyour grainM


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Re: Greetings seasoned micologists! [Re: troy_white42]
    #27138615 - 01/09/21 09:52 PM (3 years, 18 days ago)

You can not see contamination in LC. So you won't know the LC is bad until you use it. Grain on the other hand you will most likely see the contamination and smell it.


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Re: Greetings seasoned micologists! [Re: SHROOMSISAY01]
    #27142882 - 01/11/21 11:18 PM (3 years, 16 days ago)

First pins on some pink and bear head.

https://ibb.co/jWVwDNS

https://ibb.co/8r8MZM6


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Re: Greetings seasoned micologists! [Re: troy_white42]
    #27143015 - 01/12/21 12:58 AM (3 years, 16 days ago)

:cheers:


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Re: Greetings seasoned micologists! [Re: teknix]
    #27143382 - 01/12/21 09:03 AM (3 years, 16 days ago)

Wow, I love how you really went all in on this set up. I started around the same time but went with a small 3x3 tent and single disc fogger. Nice to see goals to work toward.

I'm still a complete noob and haven't been burned by LC yet so I'm with you on how great I found it to be but it seems anyone that has done this for longer moves away from it. To me using clean agar to make LC almost seems too easy for expansion. I'm still new to agar and the condensation is driving me crazy as well.

Quick question on why you are using WBS instead of whole oats or rye for spawn? I found oats to be really cheap and super easy for making spawn.


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Re: Greetings seasoned micologists! [Re: ShutEye]
    #27143492 - 01/12/21 10:15 AM (3 years, 16 days ago)

So I started small and it was easier to manage but I am looking to large scale long term and start farmers marketing and selling to local gourmet restaurants.  I've got the space so I figured might as well.  I started small with a single fogger and small tent so I could learn from my mistakes before investing larger scale. I really had a vague idea of what I needed to invest in until I did a trial run and made the mistakes.  Not to say I've perfected my skills at all but I think I've got the base science down enough to upgrade and start tweaking a large tent.

I'm going to give g2g a shot next batch since I made a bag of wbs for each species.  I'm not super confident in it only because my flow hood is tiny and dropping a grand into a real one isn't really on the budget atm.  I can definitely see the potential for g2g but I might stick to LC bag injects till then.  I'm hoping to take a LT sample from these first grows and needle inject it into LC for a 100% for sure clean LC grow for the next generation.

I use wbs because I can get a 40lb sack of it for $10 and I can only find horse Oats for about a $1/lbs and/or order rye berries for more.  I did some pint jars of wbs and didn't really see an issue colonizing.  I might order some rye berries or test the horse Oats just to see if it's a growth rate thing later on but again it's a budget thing.  I'm mostly going to just use wbs for pint jars to knock up with my LC along with a few agar plates to check quality control.


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Re: Greetings seasoned micologists! [Re: troy_white42]
    #27143938 - 01/12/21 01:57 PM (3 years, 16 days ago)

Your mushrooms will tell you if it is enough. If you start getting long stems it is time for a circulation fan. For the most part, you need a circulation fan it will keep stagnating air from building up around the mushrooms.


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Re: Greetings seasoned micologists! [Re: SHROOMSISAY01]
    #27145318 - 01/13/21 08:21 AM (3 years, 15 days ago)

Note to self: don't fall asleep with the PC on.  Woke up to my bags melting.  Should be a fun job cleaning the pot later 🙄.  Got some free time later today. Gonna try and finish my tent construction so I can get these fruiting bags off the counter and properly hydrated.  Bout tired of hand misting them.  Fruiting nice though!  Dropped some test drips from my new 32oz LC jars onto agar since they look pretty colonized for a test run.  They look nice in the jars lwts see how they do on the agar.  So I learned to label my agar preinjection.  Trying to figure out how to parafilm them still..  when you move the wedge the liquid from the syringe moves all over the plate making it grow all over instead of from your control point.  🤬.  Learned you can autoclave syringes which is super nice since with a lot of species you go thru a shit ton testing agar and injecting bags/grain.

  Fish food safeguard didn't seem to do much for clearing up my junk jar.  Ick guard seemed to clear up one a bit.  Testing the mycelium on agar now.  Got some fish mox coming and ill test that on another junk jar and agar it soon.  Tryibg to solve my budget problem in order to g2g the wbs I got going and guess I'm going to try the box fan/filter approach as my laminar isn't that big to help give me more space.  Ik it's not true laminar and thr filter won't be 99.9 but it's better than nothing for now.

  Ordered a second PC so I can roll 8 bags an hour instead of the tedious 4.  Def need a third in time and eventually upgrade to. Double barrel steamer but that's a hefty project and will take some time.  Future thoughts.  Also think I'm going to cross breed all these test agar plates since I'll have a shit ton and see if I can get any mates.  Cuz why not?


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Re: Greetings seasoned micologists! [Re: troy_white42]
    #27145463 - 01/13/21 09:36 AM (3 years, 15 days ago)

What type of PC are you using? I fell asleep once cooking agar, luckily caught it before harming the autoclave itself.. just a way overcooked jar of agar.. at this point i set an alarm for each step on the autoclave, for instance as soon as i turn it on i set a 25 minute timer so i remember to come check if its steaming. i have forgotten about it too many times. :lol:


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Re: Greetings seasoned micologists! [Re: deadmandave]
    #27145504 - 01/13/21 09:54 AM (3 years, 15 days ago)

Presto 23 quart.  I usually set an alarm too but I worked late and figured I'd get one batch done before bed and forgot.  The bottle of wine probably didn't help 😆.  The pot doesn't look too bad but I'll prob have to take it outside and heat it up to get all the plastic off that melted.    Worse part is the house smells like rubber for a few days lol.


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Re: Greetings seasoned micologists! [Re: troy_white42]
    #27145977 - 01/13/21 01:36 PM (3 years, 15 days ago)

Make yourself a hotplate with PID controller and timer. Press a button and you can sleep all you want. I have mine set up to sound a very loud alarm when it reaches 140F so if I am doing agar I know when to pour it. Just get a broken stove from craigslist and you will have all the parts you need except the timer and PID. Your PC will use less water also if set up correctly I can run mine for 4 hours with no problem...




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Re: Greetings seasoned micologists! [Re: SHROOMSISAY01]
    #27146052 - 01/13/21 02:26 PM (3 years, 15 days ago)

I'll probably build one off your tek when I get my tent situated and first mass bags colonizing.  Just a matter of free time atm lmao


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Re: Greetings seasoned micologists! [Re: troy_white42]
    #27146154 - 01/13/21 03:48 PM (3 years, 15 days ago)

I don't think I made a tek on that one but I probably should. It really makes working with your PC so easy no more adjusting or having to keep an eye on it.


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Re: Greetings seasoned micologists! [Re: SHROOMSISAY01]
    #27148372 - 01/14/21 06:36 PM (3 years, 14 days ago)

Finally got a few days free from working OT and made some major progress on my tent.  Just got to build a door which is being hinged on and the air seal with gorilla.glued velcro.  Still got to build the shelves but I got the space shelf Frame almost up too.

https://ibb.co/JsDnFDh
https://ibb.co/z5xyfV1

Got my modified drum fogger lined in with two pvc entries.  The flapper is built in up top to draw in FAE from the suction of the - pressure.  Last pic is the - pressure exhaust fan porting put the window.

https://ibb.co/smYcZYR
https://ibb.co/N35NtvN
https://ibb.co/v1ZGtWD

My first aggressive species FO and PO pins are doing well especially since I've been stuck hand misting them.  Pain in the ass.  Ready to get atmosphere in my tent.  So I'm looking at this FO and it's looking like BO so either I got sent a wrong syringe or I was just shit faced when I injected it and/or labeled it.  Who knows..  I'll have to figure it out.

https://ibb.co/18r14Tc
https://ibb.co/FxcLtG8
https://ibb.co/4mQzcJV

Agar test plates from my LC jars are starting to grow.  Prob a.couple more days before I can tell.  More to come.


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Re: Greetings seasoned micologists! [Re: troy_white42]
    #27151034 - 01/16/21 07:55 AM (3 years, 12 days ago)

Atmosphere!  Running at 92% humidity pretty easy yay.  Mightbtest bump it up but I gotta figure out my drainage problem from the pipes drip.  Got my first couple shelves up and fruiting nicely!  Smooth sailing from here!

https://ibb.co/LQ7VbJD
https://ibb.co/2gQmD3K
https://ibb.co/y0xBWmT
https://ibb.co/Gc7Jx8F
https://ibb.co/GkzBKc3
https://ibb.co/QpBkqMm

How do you know they're done growing and ready to be harvested?


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Re: Greetings seasoned micologists! [Re: troy_white42]
    #27152310 - 01/16/21 10:02 PM (3 years, 11 days ago)

Quote:

troy_white42 said:
Smooth sailing from here!




Famous last words...


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Re: Greetings seasoned micologists! [Re: taku]
    #27154123 - 01/17/21 07:38 PM (3 years, 10 days ago)

Made another slew of agar plates today.  Testibg each of my LC jars before they get the quality control for a 👌.  I've made about 90 plates and getting better.  Learned to not just suck up LC but get a concentrated pull from the mycelium otherwise you take a chance of just shooting sugar water on your plate and nothing grows. 

Also kept my dirty jar plates that I dumped and remade to learn how different bacteria/yeast grows and what it looks like from begging to end.  The pink yeast is really pretty tho lol.  It's only a mistake if you don't learn from it.

https://ibb.co/DDqjSc9


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Re: Greetings seasoned micologists! [Re: troy_white42]
    #27154925 - 01/18/21 08:28 AM (3 years, 10 days ago)

First couple of harvests from my first batch of sloppy bags.  PO, FO, and EO.  Bags were about 4lbs.

https://ibb.co/wzLX3y8
https://ibb.co/Z6JbR8X
https://ibb.co/RPY98qK
https://ibb.co/bWhHfkh
https://ibb.co/kh64FXM


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Re: Greetings seasoned micologists! [Re: troy_white42] * 2
    #27155579 - 01/18/21 02:14 PM (3 years, 10 days ago)

Please learn how to post pics properly it makes everything much easier.


1. Click on (PICS)...



2. Click on (UPLOAD)...



3. Click on (CHOOSE FILES)...



4. Once you click on choose files select where the picture you want to put on shroomery is located...



5. Choose your file you want to upload from the right side of where the red box is in the above pic.

6. After you choose your picture it will go into this slot. You can choose another pic by following the same process and clicking image 2 choose file...



7. Click upload...



8. Click on the text under the pic it will turn blue and say copied...



9. Past the copied text where you want your picture to show up on shroomery.

DONE

.
.
.
.
.
.


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Re: Greetings seasoned micologists! [Re: SHROOMSISAY01]
    #27156258 - 01/18/21 08:28 PM (3 years, 9 days ago)

So I did a thing.  I got tired of wasting time  mixing bags in a 5g bucket. 

Made in China so the instructions were shit and I had like 5 extra screws but I works.  Problem is the seal gasket sucks and dumped water on the floor.  I have to mix and pour so it sucks up the water as it spins.  Can mix a 40pb bag with 24 cups of wheat bran and 96 oz of water in one go while I do other shit.



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Re: Greetings seasoned micologists! [Re: troy_white42]
    #27156330 - 01/18/21 09:28 PM (3 years, 9 days ago)

watching you do all this fucking makes my day troy. rawr!


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Re: Greetings seasoned micologists! [Re: troy_white42]
    #27156351 - 01/18/21 09:41 PM (3 years, 9 days ago)

Quote:

troy_white42 said:
So I did a thing.  I got tired of wasting time  mixing bags in a 5g bucket. 

Made in China so the instructions were shit and I had like 5 extra screws but I works.  Problem is the seal gasket sucks and dumped water on the floor.  I have to mix and pour so it sucks up the water as it spins.  Can mix a 40pb bag with 24 cups of wheat bran and 96 oz of water in one go while I do other shit.







why mix twice if you mix when you add water you still have to mix when youspawn. I put the proper amount of my sub mix in and then I just use a zj-lcd-m flow meter to put the water in. I then sterilize and let cool when I spawn I just mix a little and I am done. Mix the dry sub mix in the bag so there is very little mixing when you spawn. The heat makes the pellets mix  and break up easy.


Edited by SHROOMSISAY01 (01/19/21 01:24 AM)


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Re: Greetings seasoned micologists! [Re: SHROOMSISAY01]
    #27156463 - 01/18/21 11:00 PM (3 years, 9 days ago)

96oz of water...you must have made a typo


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Re: Greetings seasoned micologists! [Re: Quadman]
    #27156956 - 01/19/21 07:53 AM (3 years, 9 days ago)

96 cups my bad.  I tried mixing the wood in the bags on my first batches and found inconsistencies in the mix.  I also overfill my bags to the brim just below the filter so there's not much room to mix especially after heat sealing all the air out.  Just gonna break up the mycelium at 50% and push it around a bit.  I usually create a slide on the side of the bag by pinching it and inject the LC down the side so it reaches the bottom.  I also had a problem trying to mix substrate in the bags where it contorted the plastic a bit and gave my hear sealer trouble making a seal.  Pin hole air spots on the seal mase a few bags go contam.  Either way saving myself a lot of time hand measuring a bag at a time until I decide to build a loading station.  Kind of doing things in increments because it's super overwhelming at this scale haha.  But I'm chipping away at progress and I'm growing pretty big.

Got 150 new 32 oz jars coming that I need to drill lids.  So that puts me at 174 jars.  I started using a turkey injector 1oz syringe with an 18g to inject bags.  So that's about 30 bags per jar.  Will have a nice stock pile to pull from with less downtime waiting on my jars to colonize.  Been trying to figure out how I can do g2g and just can't seem to get past throwing a grand at a real flow hood.  Future tense it's going down but I need to recoup some $$ at this point if the investment.  Think I'm going to try and push some agar plates and syringes on Amazon, ebay, and etsy with my site I'm building.  www.mushlov3.com


Edited by troy_white42 (01/19/21 08:07 AM)


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Re: Greetings seasoned micologists! [Re: troy_white42]
    #27157028 - 01/19/21 08:47 AM (3 years, 9 days ago)

You dont push air out at sealing after inoculation. You should add air if your at a flow hood. This will allow for mixing and air for quicker colonization.


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Re: Greetings seasoned micologists! [Re: Quadman]
    #27157050 - 01/19/21 09:04 AM (3 years, 9 days ago)

Ya, I push air out prior to seal and pressure cook to make room in the PC.  My tiny lil flowhood is really only big enough to handle jar and agar work.  I need to invest into a double 24x24 eventually so I can work with open bags without worry.  Growing pains


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Re: Greetings seasoned micologists! [Re: troy_white42] * 1
    #27166328 - 01/23/21 10:08 PM (3 years, 4 days ago)

Went for a hike today and salvaged 2.5gallons of fresh looks like brown oyster.



Along with some turkey tail and witch butter.


Edited by troy_white42 (01/23/21 10:10 PM)


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Re: Greetings seasoned micologists! [Re: troy_white42]
    #27191274 - 02/07/21 09:22 AM (2 years, 11 months ago)

All right so been a while I've been tinkering and learning.  Heres some.current problems:

LC jars I've learned to gasket seal both sides of the nipples because I'm paranoid and want to make sure no contams.  I can't figure out if my contams coming from my noob laminar hood or from in thr jar.  I get a little trich or green mold on about 1/5 plates that I have to clean.  I've got baby food jars sealed with hwfp that I'm baby fruiting to test.  Most look clean so far colonizing.

My tents finished and up and running.  My 6 disc fogger blew out the top 3 discs.  I'm assuming overheat from running too much.  I built a monotub and put a single disc fogger inside the tent to compensate and run 10 on 2 off.  HOH sent me a replacement6 disc free as long as I cut the cord on the old one.  I can resolder the wire and probablyrepair the motherboardwhen I have time so win.  Just need a new power supply.

Lions mane are growing big but start to brown before they teeth.  I'm thinking lack of humidity.  Hopefully the new fogger set up will compensat.

Chicken, shitake, and maitake.  Bags are turning orange and brown as they should but I don't see any pins.  The chickenis getting lobes but they seem to take forever.

Isolated and cloned some TT and wild oysters on agar then to LC.  Also did a few test runs with LT to LC which I like.  It seems much cleaner and grows pretty quick.  I'll prob swap all my jars to LT LC future tense.


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Re: Greetings seasoned micologists! [Re: troy_white42]
    #27191432 - 02/07/21 10:48 AM (2 years, 11 months ago)

I have a power supply for a 9 disk mister that I would guess would work. You pay to ship and it is yours. PM me so we can make sure it will work for you. Both my 9 disk fogger from HOH crapped out after about 3 months so I would just do something that will last or you will always have problems. High-pressure mist is the way to go I got the kit you need.


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Re: Greetings seasoned micologists! [Re: SHROOMSISAY01]
    #27194968 - 02/09/21 07:54 AM (2 years, 11 months ago)



So all my initial bags are on their last flushes.  They did pretty well considering some were only half colonized and a few had contam.  I wanted to run them to gauge and learn.  Also helped me read my tent and adjust fae and humidity for the next slew of bags.  I've got a dozen shitake and maitakke colonizing atm and gonna make a slew of oyster bags to go in as well.

Mostly been organizing and building.  Had to make shelves for my desk as it was getting cluttered with all the LC jars.  I've got at least 2 jars per species.  They go here first then when colonized and tested on a pint jar where they fruit into the closet to colonize completely then into a garage fridge for storage.  I've also got a mini fridge next to my desk where I'm storing 10+ colonized plates of each species and various slants/backups.

Been playing with a Graham condesor making hydrosol for when I get reishi and stuff going.  Going to do some double extraction and see what can be done with distilling.  Worse case I can still make essential oil.  Picked up some superworms.  Converted an old aquarium into a habitat that will house the birthed larvae beetles to reproduce more superworms.  Colonizing some cordyceps that I'm going to let parasite the larvae to fruit.  So I'm wondering.. do the insect actually need to eat the mycelium to get infected or can you inject a larvae or dead host.

Also figured I'd start some variations of peppers to throw in the grow tent.  I've got plenty of room and figured they'd help with co2.  Trying to find some LED strips that aren't super expensive to help with the light for them.  Working on a fountain pump to run micromisters set on a sumer switch for auto water.


Edited by troy_white42 (02/09/21 11:46 AM)


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Re: Greetings seasoned micologists! [Re: troy_white42]
    #27295880 - 05/05/21 05:35 PM (2 years, 8 months ago)

Alright, fibially got this down to a science for oysters and lion mane.



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Re: Greetings seasoned micologists! [Re: troy_white42]
    #27296068 - 05/05/21 07:14 PM (2 years, 8 months ago)

nice man! the teeth in that first pic look a bit long, consider picking earlier. love how you just do the things, all the things!


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Re: Greetings seasoned micologists! [Re: taku]
    #27296744 - 05/06/21 08:40 AM (2 years, 8 months ago)

Ya man thanks!  I usually pick earlier but I've got too many so I just let er rip. Lol.  My tents fully automated now and the only slow part is bagging.  I'm to the point I need to invest in a real flowhood but ugh the money.  Hows your buckets going?


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Re: Greetings seasoned micologists! [Re: troy_white42]
    #27297237 - 05/06/21 03:49 PM (2 years, 8 months ago)

Ha I give you a month before you cave. Look how much you've done so far! At least it's a purchase you'll never regret.

Buckets are out and blocks are in. I'm just figuring them out and ramping up production. Trying out some new strains and starting to sell my mushrooms which is really exciting. Still loving the myco ride, and finding a rhythm to the work. That's a big part of it I'm finding.


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Re: Greetings seasoned micologists! [Re: taku]
    #27297434 - 05/06/21 06:24 PM (2 years, 8 months ago)

I've been selling for $1/oz but it's hard to find a steady market.  Just a few people here and there and a few once a month regulars.  I've mostly been doing buy/sell groups on local Facebooks and neighbors.  Been considering a farmers market but idk if I feel like sitting out there all day. 

The fresh 4-5 day is a bitch.  Like trying to sell them before they don't look as appealing is the hard part.  I've got lbs and lbs in my fridge and I've been incorporating them into all kinds of new recipes.

Interestingly enough I've started to puree the oysters and they make a great meat substitute.  I'm not vegan so I use like half meat half oysters.  IE: made some enchiladas half meat half puree, some jambalaya half 1/3 puree chicken sausage.  Got tired of Sautee and deep fry.  Gotta learn how to start drying them next I guess.

Also got about 3 gallons of Lion mane double extraction I made.  Been selling to my hippie friends $20 for a 2oz dropper bottle.  Not much bite on the Facebook groups.  Debating amazon because the monthly fee.  Whats your plans?


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Re: Greetings seasoned micologists! [Re: troy_white42]
    #27297655 - 05/06/21 08:31 PM (2 years, 8 months ago)

I went direct to my local grocers selling to them at $8/lb and they retail $10/lb. 20% margin makes them happy and I can offload all my mushrooms. I also network just when out and about. Have a health food store wanting dried lion's mane. So right now I'm mostly planning on grocers and niche suppliers. Will approach restaurants when i get more lbs/week.

I'm finding a big part of it is marketing how to use mushrooms. People are just used to white button mushrooms where I live and are thrown by anything different.

Farmers markets are also a goal, we have a few big ones out near me. And then it helps that my family runs a u-pick Berry farm/orchard that brings in city folk all summer that I plan to sell to. 

I would say dry your excess mushrooms. I just use a regular cheap dehydrator to get them cracker dry. Can buy bags to package easy on Amazon. Look for Kraft bags. Dry them before too old so they don't get bitter. Had good advice about golden oysters - super bitter after about 3-4 days.

It's also good to teach your grocers when supplying as they may have no clue either. I gave them a general timeframe for how long before they should remove them and they gave me.pointers for marketing my mushrooms with easy recipes and what amounts their customers would want - I do 4oz containers. Small batches for simple sides and extra flavor in dishes.


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Re: Greetings seasoned micologists! [Re: troy_white42]
    #27301063 - 05/09/21 03:55 AM (2 years, 8 months ago)

Quote:

Quadman said:
You dont push air out at sealing after inoculation. You should add air if your at a flow hood. This will allow for mixing and air for quicker colonization.




Oh snap what's up Quad! :wave: good to see you mate!! I agree with this statement wholly.
Quote:

troy_white42 said:
I've been selling for $1/oz but it's hard to find a steady market.  Just a few people here and there and a few once a month regulars.  I've mostly been doing buy/sell groups on local Facebooks and neighbors.  Been considering a farmers market but idk if I feel like sitting out there all day. 

The fresh 4-5 day is a bitch.  Like trying to sell them before they don't look as appealing is the hard part.  I've got lbs and lbs in my fridge and I've been incorporating them into all kinds of new recipes.

Interestingly enough I've started to puree the oysters and they make a great meat substitute.  I'm not vegan so I use like half meat half oysters.  IE: made some enchiladas half meat half puree, some jambalaya half 1/3 puree chicken sausage.  Got tired of Sautee and deep fry.  Gotta learn how to start drying them next I guess.

Also got about 3 gallons of Lion mane double extraction I made.  Been selling to my hippie friends $20 for a 2oz dropper bottle.  Not much bite on the Facebook groups.  Debating amazon because the monthly fee.  Whats your plans?



Trying to find a market can be a hastle- but when you go to restaurants to try to sell them- don't forget about the line cooks. When I had my farm I didn't have any restaurants buying from me- but my top customers were line cooks from several restaurants. The store itself may not want to branch out and try your product but their staff may be all about it.


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