|
SHROOMSISAY01
Mr. Shrooms


Registered: 01/22/17
Posts: 3,849
Loc: Virginia, USA
Last seen: 1 hour, 27 minutes
|
Re: Greetings seasoned micologists! [Re: taku]
#27079580 - 12/08/20 09:50 PM (3 years, 1 month ago) |
|
|
Yea you want a fan inside running all the time but not blowing on the mushrooms. That little bit of fan blowing the humidity in is not enough.
|
troy_white42
Stranger

Registered: 12/04/20
Posts: 150
Last seen: 1 year, 8 months
|
|
Amazon and an entire weekend later I've got some major first timer status going. Hoping all goes well but I'm sure I'll run into some hurdles. So about 30 bags sealed, mixed, filtered, and gaskets. All PC for an hour and knocked up with about 2cc of LC from a vendor. Kncoked up 2 half pints each of wbs and homemade LC mix with 1cc of the same syringe that I also PC for backup. 13 species mostly oyster variants and LM/BH.
Got about 1cc left in each syringe that I'm saving to knock up some baby food jars for agar. Gonna PC them with ports and filters. Keeping it all in my spare room at about 70-75°. Gonna work on my fruiting chamber while they do there thing but I'm excited. What am I forgetting?
https://ibb.co/GF5kqyn
https://ibb.co/4KF7rsC
Oh, I've got a 99.9 12×12 filter that I'm going to build a lamar hood too. Just wanted to kncok the bags up. Timing issue with amazon.. I will do everything lamar next go around. Hoping the contam isn't too bad firing the needles.
Edited by troy_white42 (12/13/20 08:33 PM)
|
troy_white42
Stranger

Registered: 12/04/20
Posts: 150
Last seen: 1 year, 8 months
|
Re: Greetings seasoned micologists! [Re: troy_white42]
#27090968 - 12/15/20 06:16 PM (3 years, 1 month ago) |
|
|
So day 3 after injections to 30 5lb bags (5c hwfp/1.5c Rice bran/1c wbs/500ml water), 20 grain half pints (wbs), 20 half pints LC (5ml water/5grams LME/1tsp karo), 15 baby food jars agar (500ml water/5g LME/1tsp karo/agar).
Seeing whisp starters on everything from splitting a 10CC vendor syringe up 1 each with 13 species. Bh and LM none yet. Spread it thin to clone. Forgot to take pics before I shook. Doh. BO and FO seem to be the fastest sprouted with BO out front by margin. LCs seem to be growing in size and clear clumped colonies minis the degrees that o had problems with PCing.
Think I figured out the degree problem. Karo was thickening on the bottom of the pan. I poured it cold. Double coffee filtered the new LC mix for the gallon jars that I'm going to put the agar growths into once they finish out and I get my lamar built. Excited to see baby progress
Decided to polish off some Jameson and build the box.it ain't pretty but it's air tight. Got a floor fan and filter to spec the cubic foot and 99.9%. Waiting on the silicon to dry to give er a test run.
https://ibb.co/zh7GSTy
Edited by troy_white42 (12/15/20 08:15 PM)
|
taku
° shapeshifter ∆



Registered: 12/05/20
Posts: 732
Loc: Canada
Last seen: 10 minutes, 24 seconds
|
Re: Greetings seasoned micologists! [Re: troy_white42]
#27091146 - 12/15/20 08:34 PM (3 years, 1 month ago) |
|
|
you should nickname that lammy "The Hammerfist" - definitely has a jameson-build vibe to it 
best of luck with your colonizing!
--------------------
|
troy_white42
Stranger

Registered: 12/04/20
Posts: 150
Last seen: 1 year, 8 months
|
Re: Greetings seasoned micologists! [Re: troy_white42]
#27100497 - 12/21/20 08:38 AM (3 years, 1 month ago) |
|
|
So my original bags have about a golf ball size growth that I've shook up. My agar have bloomed nicely and look clean except the chicken. It seems so be the slowest in par with the lion mane. Is that normal? Also, my black moral agar looks clean but the underlayer is green/black. I'm wondering if that's just normal it's super aggressive. Can't find any images of the mycelium to compare.
I've cloned the agar onto slants and made fresh LC jars from it. Waitibg to see how those turn out. Lost a shitake and maitakke jar to contam nut not bad 2/30 jars. Set them in a separate closet to study whT happens. They got all cloudy.
Knocked up another 30 bags and installed LEDs and shelves into my colonizing closet so I've got about 70 bags in there atm. The grain spawn jars are all looking nice and fuzzy. Lm and BH super slow like everything else.
So I've noticed the the bags self heating and learned about thermogenesis. Where the bags touch they conduct heat exchange and get in the upper 90s. Playing with temperature control but wondering if I should not let the bags touch. 75-85 soil temp where they aren't touching depending on how high up in the closet they are. I've got my lower temp species in the bottom where it's cooler and and higher temp like Florida oyster and gulf oyster up top.
Also have a lot of air in the bags than when I starte. Guess this is from the filter patch and co2 build up. Worried it might start pints or is that fine since it's prob all co2? Better pics to come. Just been slammed with initial build and cloning.
https://ibb.co/mG5wkwm https://ibb.co/bdNPd8H
|
SHROOMSISAY01
Mr. Shrooms


Registered: 01/22/17
Posts: 3,849
Loc: Virginia, USA
Last seen: 1 hour, 27 minutes
|
Re: Greetings seasoned micologists! [Re: troy_white42]
#27101136 - 12/21/20 03:42 PM (3 years, 1 month ago) |
|
|
LM is very thin look again closely you are probably missing it. It does not look like any other mycelium until it has set for a long time then it starts to thicken up.
|
troy_white42
Stranger

Registered: 12/04/20
Posts: 150
Last seen: 1 year, 8 months
|
|
So massive production underway. Decided to start converting a second closet to colonize bags since the first is full. My initial bags are starting to colonize. Some slower than others but the pinks, Florida's and elms are taking off. The shitake, mistake, chickens, and manes are slow but showing growth. Does the last bag look too dry? I was thinking about injecting like 100cc of my clean LC broth to rehydrate? https://ibb.co/GVnwJ31 https://ibb.co/VBsL0S8 https://ibb.co/7YKFY3S https://ibb.co/0h0Chxv
Converted a half closet for my agar, and LC mixes. Lost about 3 jars from my original batches but learned a lot of sterile tek since and ordered some 32oz jars to xfer the agar to lc since my first batch was just LC to LC. Also learned how to get the damn sediment out of my LC. It was either the LME or karo after PCing. Double coffee filtered it after and recoooked for a clean LC. Most of the manes seem to not want to spread across the agar and just bubble up. I'm not sure if it's forcing fruiting or just mycelium?
https://ibb.co/NSv40BX https://ibb.co/tCfMDgZ https://ibb.co/s3p43zK https://ibb.co/JRtYpcS https://ibb.co/RYwH2Bd
The wbs jars are all doing well and some looking like fully colonized. I don't think I trust my skill to G2G my bags yet so I'll stick to LC injections with sealed bags. Not quite understanding how to keep it sterile with just folding a tyvek sleeve. Might practice when I catch up. This jar of BH looks like it's fruiting or is that just mycelium?
https://ibb.co/JrgKkYN
Working on my fruiting chamber om my cloning and PCing breaks. Its coming along. Almost ready to test run the humidity fogger tub and see what I can regulate with FAE fans. More to come.
https://ibb.co/j6XMmpF
|
troy_white42
Stranger

Registered: 12/04/20
Posts: 150
Last seen: 1 year, 8 months
|
Re: Greetings seasoned micologists! [Re: troy_white42]
#27124565 - 01/03/21 07:17 PM (3 years, 25 days ago) |
|
|
So learned a lot about proper bag seals. I was mixing my hwfp/bran in the bags and then sealing. That left micro holes from stretching the plastic when sealing. Noticing contam in a lot of bags. Sucks since I made about 100 that way. Oh well! Made another 50 with proper seals. Also, note to self wbs germinates.. use it before you lose it.
Learning agar. It's a pain but it's legit for identifying if your batches are bad and isolating. Need a bigger hood. Now that I've got my jars built up I've graduated to 32oz jars with drilled injection ports and filters not just jb weld glued filters and gasket maker. Blew a few on first PC and learned to seal both sides of the lids.
My first grain jars finished and I tested them on substrate bags. Totally exploded. Worth the time. Got 5lb bags of wbs cooking atm to knock up for g2g xfer on the next go. Wondering how to get around the xfer from pressure cooker to.flow hood for contam since it'll only have a tyvec sleeve.
|
SHROOMSISAY01
Mr. Shrooms


Registered: 01/22/17
Posts: 3,849
Loc: Virginia, USA
Last seen: 1 hour, 27 minutes
|
Re: Greetings seasoned micologists! [Re: troy_white42]
#27124692 - 01/03/21 08:26 PM (3 years, 25 days ago) |
|
|
No Tyvek sleeve is needed just fold the bags properly they will be sealed so no need to worry about moving them from the PC to the hood...
&ab_channel=ChuckTaylor
|
Possum-Pie
Stranger


Registered: 12/03/20
Posts: 57
Last seen: 2 years, 9 months
|
Re: Greetings seasoned micologists! [Re: troy_white42]
#27125210 - 01/04/21 04:17 AM (3 years, 24 days ago) |
|
|
Quote:
troy_white42 said: So learned a lot about proper bag seals. I was mixing my hwfp/bran in the bags and then sealing. That left micro holes from stretching the plastic when sealing. Noticing contam in a lot of bags. Sucks since I made about 100 that way. Oh well! Made another 50 with proper seals. Also, note to self wbs germinates.. use it before you lose it.
Learning agar. It's a pain but it's legit for identifying if your batches are bad and isolating. Need a bigger hood. Now that I've got my jars built up I've graduated to 32oz jars with drilled injection ports and filters not just jb weld glued filters and gasket maker. Blew a few on first PC and learned to seal both sides of the lids.
My first grain jars finished and I tested them on substrate bags. Totally exploded. Worth the time. Got 5lb bags of wbs cooking atm to knock up for g2g xfer on the next go. Wondering how to get around the xfer from pressure cooker to.flow hood for contam since it'll only have a tyvec sleeve.
I've been following your progress with your new hobby on this thread. I have to admire you jumping in with both feet right off the bat. I too am relatively new to mycology, but I have started in a timid manner, 3 bags of each species, writing everything I do and the dates in a journal in case I need to find out where I failed. A few observations: YES If you have that many bags in a relatively small space you definitely need to separate them for airflow as exothermic reactions will make them hot. My 3 bags in a large room give negligible heat but the number you have will get hot. I read about sealing your bags and it seems you are doing it too soon. I mix, fold down the tops (Never used Tyvek they don't stick) and stack them unsealed into the PC. After they cool overnight with the lid still on, I take them out one at a time, inoculate them, heat seal the tops and shelve them. YES, I too have had the heat sealer melt small holes in the Unicorn bags once, but I'm unlatching the heat sealer a few seconds before the light goes off and have stopped having holes. Never yet had contamination. As for the "Air" in the bags, it is a mixture of room air, with added CO2 as the mycelium respirates. I made a huge mistake the first batch of Shiitake I did by over-filling the bags. CO2 couldn't transpire fast enough with little head-space in bags and it died. I'll be watching your progress!! I have my first harvest this week of Maitake which has been a slow process but has actually worked! Now I am getting ready to re-try Shiitake after every bag failed to fruit the first attempt I made.
|
troy_white42
Stranger

Registered: 12/04/20
Posts: 150
Last seen: 1 year, 8 months
|
Re: Greetings seasoned micologists! [Re: Possum-Pie]
#27126031 - 01/04/21 12:07 PM (3 years, 24 days ago) |
|
|
The fold and dash from the kitchen pc to the lab bedroom makes me nervous lol. I guess I'll give it a go when these new 5lb wbs colonize for g2g xfers. Injectioning 10 of them today with different species. Plan on using those to knock up future bags rather than LC injections. Only gonna use LC when I'm out of grain and need bags.
Ya, I figured the bigger production would give me a bigger test groups to learn from. I've definitely learned a lot in this month of growing so thanks! I'm hoping to start fruiting a few BO and PO next week fingers crossed. Will get a chance to test my fruiting chamber in action which btw I switched from a 1 disc to a 6 disc fogger. It should be here this week. Good luck possum! More pics to come
|
SHROOMSISAY01
Mr. Shrooms


Registered: 01/22/17
Posts: 3,849
Loc: Virginia, USA
Last seen: 1 hour, 27 minutes
|
Re: Greetings seasoned micologists! [Re: troy_white42]
#27127356 - 01/05/21 01:54 AM (3 years, 23 days ago) |
|
|
wait I am confused you are fruiting pink oyster and blue oyster in the same room? Pink is warm weather and blue is cold weather.
|
troy_white42
Stranger

Registered: 12/04/20
Posts: 150
Last seen: 1 year, 8 months
|
|
I've got 13 different species colonizing. Going to test run all of them. Closing some vents off and stuff for different temp zones.
|
SHROOMSISAY01
Mr. Shrooms


Registered: 01/22/17
Posts: 3,849
Loc: Virginia, USA
Last seen: 1 hour, 27 minutes
|
Re: Greetings seasoned micologists! [Re: troy_white42]
#27128648 - 01/05/21 04:16 PM (3 years, 23 days ago) |
|
|
Ok, I understand. I just grow what is in season that way no controlling temps.
|
troy_white42
Stranger

Registered: 12/04/20
Posts: 150
Last seen: 1 year, 8 months
|
|
Alrighty, got my new 32oz jars in and cooked up 24 jars of LC using 5g LME 1tsp karo per 500ml water. The next batch I'm swapping corn dextrose for karo after some research. 2 jars per species knocked up with a master agar that I grew from the original syringe. They're looking good after just a couple days. Here's the LM
https://ibb.co/kc5VMmd
Starting my slurry of agar plates now to test each jar for contams. Dropping a drip of LC to 3 agar plates for each jar so that's 36 plates. Fml. Side note: the condensation on the lids really piss me off and I'm trying to find work around to remove it without having to wait for the agar to cool to almost gel state prior to pour.
https://ibb.co/2YRJWxW
Pulled a test bag of black morel that's colonized. I zip tied below the filter cut some slits and throwing it in the fridge for a couple weeks to see what happens. I heard the nay sayers but that just made me want to try more.
https://ibb.co/pPM6QTy
Also cooked up 13 5lb bags of WBS and knocked em up with LC to start my G2G for next round of bags. Liked the growth rate explosions and I figure it can't hurt to get a round of grain going while these bags are doing there thing. Need to finish the agar, monitor them, and verify clean LC jars then I should have 180+ syringe pulls per species! I'll probably grow the agar in my smaller half pints jars after to fruit and verify species. Considering scrapping the self contained shelf green houses and just converting the room into a greenhouse tent. Wondering if the 6 disc fogger will work for a 15×15 room.
|
troy_white42
Stranger

Registered: 12/04/20
Posts: 150
Last seen: 1 year, 8 months
|
Re: Greetings seasoned micologists! [Re: troy_white42]
#27134740 - 01/08/21 08:48 AM (3 years, 20 days ago) |
|
|
Scraped my 10g tupperware box and found a cheap plastic plastic 55g drum from a local car wash. Mounted the inline fan cut a water hole and installed a door/seal on it to put in the water and fit the 6 disc fogger inside. JB quick welded a pvc joint onto the lid so I can run my pvc out. Works pretty well on an initial test run! I'm stoked to get it piped to the tent and see what it can do.
All my new jars are starting to colonize well but I'm pretty sure my agar had contam on my chicken LS cuz they went all murky. Set them aside to assess a few days. Gonna throw my original bags in the fruiting chamber in the next couple days even though they had poor seals and possible contams just to see what they do.
https://ibb.co/CKj7phM
|
troy_white42
Stranger

Registered: 12/04/20
Posts: 150
Last seen: 1 year, 8 months
|
Re: Greetings seasoned micologists! [Re: troy_white42]
#27137528 - 01/09/21 11:55 AM (3 years, 19 days ago) |
|
|
Scrapping the shelf tent idea and using 2x4x8s the frame 10x8x9 tent in the room. Its just less work and more sq ft for a fruiting chamber. The shelve is just a pain in the ass to open and close. Using 3mm 25x10 plastic sheeting to make the tent walls and going to gorilla glue a velcro door to access. Converted a 55g drum ($25 from a carwash) into a humidifier with a 6 disc fogger and an inline fan with an ink bird to control RH.
https://ibb.co/C7LTgBb
Finished the window exhaust out with a flapper and inline fan to push - pressure out the window with spores. Going to install a + pressure inline with flapper on the other side both set to run on a cycle timer every 15m for FAE. Going to let my room ceiling fan do the work inside the tent for pushing around air and humidity. Not really concerned if it gets fudged in time from spores and/or humidity. The window with shades tilted inverted will do the work for light. Going to install 2x4x8 shelves with probably pvc or 1x1x8 racks to hold the bags.
https://ibb.co/553XKdJ
Since the shelf tent is already built I got it hooked up and running on 2 tiers at 97% humidity. I threw in a dozen of my most promising bags from my first trial run making bags. Some look decent. Some not so decent but I figure why not. Very least cam get a few fruits and do some LT injects into my 32oz jars later so I know I got a 100% clean LC next go around. My current LC jars i feel confident but I am still figuring out agar so it's hard to be sure.
Oh and neat idea. I got a bunch of whiskey jars sitting around so I tyvek zipped the tops and loaded them with HWFP and bran. Cooked em.up and put gaskets on them for a LC injection. Figured it would be a fun gift or just a cool grow on the counter.
https://ibb.co/wSQYKQp
|
deadmandave
Slime


Registered: 02/16/10
Posts: 3,355
Loc:
Last seen: 11 minutes, 11 seconds
|
Re: Greetings seasoned micologists! [Re: troy_white42]
#27137553 - 01/09/21 12:11 PM (3 years, 19 days ago) |
|
|
How are you gonna clean your humidifier? its pulling in room air so spores and dust etc, will be sitting in water for a while. it gets weird. not to mention bugs flying in and dying in there.
|
taku
° shapeshifter ∆



Registered: 12/05/20
Posts: 732
Loc: Canada
Last seen: 10 minutes, 24 seconds
|
Re: Greetings seasoned micologists! [Re: deadmandave]
#27137587 - 01/09/21 12:34 PM (3 years, 19 days ago) |
|
|
You, sir, are a do-er
--------------------
|
troy_white42
Stranger

Registered: 12/04/20
Posts: 150
Last seen: 1 year, 8 months
|
Re: Greetings seasoned micologists! [Re: taku]
#27137683 - 01/09/21 01:29 PM (3 years, 19 days ago) |
|
|
Haha thanks take. I like Projects. The humidifier will be outside the tent piping in air from outside. I was thinking peroxide mix in the water and dragging it outside on occasion and hosing it out.
|
|