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Invisiblecoversall
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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: verum subsequentis]
    #27063908 - 11/30/20 02:46 AM (3 years, 3 months ago)

Lol yeah, and the stuff that did land on the agar grew out ok. Clean enough for me at least.


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..:: E V E R Y  ::..

..:: New? Start here. ::..
..:: How I Panaeolus. From Agar to Tea ::..

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OfflineLuminous7


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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Josex]
    #27063914 - 11/30/20 02:50 AM (3 years, 3 months ago)

Great thread. Thank you to the poster.

Is it 100% mandatory to test LC's before use? Or if its looking good just go for it and knock jars?

Lately i think theres some bacteria hiding in my LC's because ill get 1 flush of small weird fruits then it contams with not trich nessecarily, but a dark squishy patch in the sub, followed by white molds. Also the surface of sub isnt wanting to fill out 100% before it starts knotting and pinning.  I used to push upwards of 7 flushes, i think my plates are fucked and I need to restart from spore. :frown:

But im glad i found this thread and Mads write up on Hidden Contams. So I can start correcting all this. :peace:

https://www.shroomery.org/forums/showflat.php/Number/22020260


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Invisiblecoversall
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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Luminous7]
    #27063918 - 11/30/20 02:52 AM (3 years, 3 months ago)

Quote:

Luminous7 said:


Is it 100% mandatory to test LC's before use? Or if its looking good just go for it and knock jars?






Only if you want to be 100% sure that it's clean. Otherwise just pour that shit and cross your fingers.


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..:: E V E R Y  ::..

..:: New? Start here. ::..
..:: How I Panaeolus. From Agar to Tea ::..

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InvisibleJosex
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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: coversall]
    #27063919 - 11/30/20 02:53 AM (3 years, 3 months ago)

I test them on grain jars.

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OfflineLuminous7


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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Josex]
    #27063923 - 11/30/20 02:58 AM (3 years, 3 months ago)

Quote:

Josex said:
I test them on grain jars.



I do as well sometimes. But i worry about comprimising the LC by testing it.


I do just mason jars with syringe filter disks,  and pour my LC to knock grain.


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OfflineBrownBear
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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Josex]
    #27063930 - 11/30/20 03:10 AM (3 years, 3 months ago)

Quote:

Josex said:
I test them on grain jars.




:whathesaid:

I test it on potential master grain jars. That way, if it fails there isn't much of a loss.

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OfflineLuminous7


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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: BrownBear]
    #27063933 - 11/30/20 03:14 AM (3 years, 3 months ago)

Quote:

Iambrownbear said:
Quote:

Josex said:
I test them on grain jars.




:whathesaid:

I test it on potential master grain jars. That way, if it fails there isn't much of a loss.



well if it fails on a potential GM , then you may have ruined your spawn ratio if you planned your tubs out in relation to your jars.

In the 24qt presto u can fit 10 jars as well as a little wide mouth half pint in the top center. SOmetimes i sterilize a little one of those and test LC on that.


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OfflineBrownBear
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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Luminous7]
    #27063937 - 11/30/20 03:19 AM (3 years, 3 months ago)

Quote:

Luminous7 said:
Quote:

Iambrownbear said:
Quote:

Josex said:
I test them on grain jars.




:whathesaid:

I test it on potential master grain jars. That way, if it fails there isn't much of a loss.



well if it fails on a potential GM , then you may have ruined your spawn ratio if you planned your tubs out in relation to your jars.

In the 24qt presto u can fit 10 jars as well as a little wide mouth half pint in the top center. SOmetimes i sterilize a little one of those and test LC on that.




I use 1/4 pint jars of rice for masters and cook them 24+ plus at a time. I use one 1/4 pint to inoculate 3 qt grain bags. So to put it simply, I make more masters than what I need. The ones that don't make the cut get mixed into a shoe box grow or something similar.

Edited by BrownBear (11/30/20 03:46 AM)

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OfflineLuminous7


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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: BrownBear]
    #27063951 - 11/30/20 03:55 AM (3 years, 3 months ago)

Quote:

Iambrownbear said:
Quote:

Luminous7 said:
Quote:

Iambrownbear said:
Quote:

Josex said:
I test them on grain jars.




:whathesaid:

I test it on potential master grain jars. That way, if it fails there isn't much of a loss.



well if it fails on a potential GM , then you may have ruined your spawn ratio if you planned your tubs out in relation to your jars.

In the 24qt presto u can fit 10 jars as well as a little wide mouth half pint in the top center. SOmetimes i sterilize a little one of those and test LC on that.




I use 1/4 pint jars of rice for masters and cook them 24+ plus at a time. I use one 1/4 pint to inoculate 3 qt grain bags. So to put it simply, I make more masters than what I need. The ones that don't make the cut get mixed into a shoe box grow or something similar.



Interesting , never thought to do something like that. Cool idea!

do you use LC ?

Lately iv been making mass amounts of LC, like a few litres at a time, lets say 4000ml. Ill use quart mason jars and do 500ml per jar.

Im starting to wonder if i should just be doing g2g instead of mass amounts of liquid culture.  LC seems like more potential for error than G2G. I havnt done much G2G work.


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OfflineBrownBear
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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Luminous7]
    #27063957 - 11/30/20 04:19 AM (3 years, 3 months ago)

Yes. I use lc. I was growing lc in quart jars but have recently switched to the pint jars. I grow a few lc's of each culture and use the best one out of each culture to inoculate master jars. I use 2.5 cc of lc per 1/4 pint of rice. I can inoculate a little over 5 dozen jars with one pint jar of lc.

I like both lc and g2g. I do not like lc for inoculating large amounts of grain but it is quite useful for small amounts. I like g2g for large amounts of grain because the recovery time is a lot faster, ime, with g2g than it is for lc.

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OfflineMH5109
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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: BrownBear]
    #27063999 - 11/30/20 05:28 AM (3 years, 3 months ago)

My peu and ape cultures are looking clear, here in the next few days they will be going to test jars
[/tex]


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Edited by MH5109 (11/30/20 05:28 AM)

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Offlinenmd_myco
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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: MH5109]
    #27066556 - 12/01/20 03:04 PM (3 years, 3 months ago)



This is an expansion of my first agar-QCd LC.

I used 12cc of the original 8 days ago.

I would appreciate any thoughts or opinions. I am making agar today and will start QC plates tomorrow.

Broth is 1% LME/2% Light Corn Syrup.

I also injected a test run with the source LC that already passed agar QC.


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OfflineBrownBear
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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: nmd_myco]
    #27066611 - 12/01/20 03:33 PM (3 years, 3 months ago)

That lc looks real nice. How many cc's did you inject into each bag?

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Offlinenmd_myco
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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: BrownBear]
    #27067844 - 12/02/20 09:26 AM (3 years, 3 months ago)

some of them are in 2 lb bags and some in 3lb bags. They got 6 and 9cc respectively. I was cautioned by a vendor that the biggest issue users ran into was squirting a whole spore syringe in a

This is part of a set of experiments involving sterilized grain from a pool of 7 providers.  I am running the four lowest cost providers against each other first.

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Invisiblemushhead
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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: nmd_myco]
    #27067877 - 12/02/20 09:41 AM (3 years, 3 months ago)

B+ LC tested 100% clean, the agar dish is saturated with thick white myc.
Yesterday I expanded the culture into 12 rye jars, two 5LB bags of hpoo, and 7 BRF jars.
All have recovered over night and are moving fast.

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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: mushhead]
    #27070174 - 12/03/20 02:32 PM (3 years, 3 months ago)

here's the source and QC plate for that last one i posted. its a bit late to see how clean the QC plate is.


source plate


QC plate

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OfflineSold Out Online
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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: D3_Myc]
    #27070530 - 12/03/20 06:39 PM (3 years, 3 months ago)

Quote:

D3monic said:

Did a test on agar which looks really good







What method did you use to get it to agar to test?


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Threads that have been useful to me:
Clean Spawn checklist for new growers

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Invisiblemushhead
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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: Sold Out Online]
    #27070544 - 12/03/20 06:48 PM (3 years, 3 months ago)

I just make myself a 10cc syringe of LC and test it, if its good I use it.

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Invisiblecoversall
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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: mushhead]
    #27071226 - 12/04/20 05:32 AM (3 years, 3 months ago)

I've got three pan cyan LCs that I made on the 10th of November. They have obviously been ready to pour for while. There was a hold up getting my hpoo, which I am going to finally go and dig up the muck heap on Monday.

Will these LCs be ok to pour? Or should I just start from scratch??


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..:: E V E R Y  ::..

..:: New? Start here. ::..
..:: How I Panaeolus. From Agar to Tea ::..

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InvisibleMateja
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Re: LC Training Camp: Interpreting Visual Cues To Predict The Quality Of A Broth [Re: coversall] * 1
    #27071671 - 12/04/20 11:40 AM (3 years, 3 months ago)

Colonized LC's can store for a long time in room temp and in the fridge, as long as they've not been compromised. (I don't know this personally but I've seen several growers post about  having good luck with inoculating with LC's stored at room temp for months and months)


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