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Offline6Curi0us.M0nkey9
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Agar Plate Condensation Dilemma
    #27070347 - 12/03/20 04:51 PM (3 years, 1 month ago)

So attached is a photo of an agar plate I inoculated with a MS syringe. It is doing well and I am planning on getting a bunch more plates poured after I’m done studying for finals so I can transfer it. Though, it is growing up the sides of the plate and I am afraid of it “bursting”(?) through the parafilm. I do not know how common of a phenomenon that is or if it is even in the least bit likely though I have seen pictures of it happening. I definitely want to avoid this. I put it in the fridge to stall the growth in the mean time, though an immense amount of condensation began to form. So from this situation I have roused 2 questions:

1) Is it possible for this mycelium to grow through the parafilm? If so, how likely is it to occur?

2) Is the condensation going to harm the mycelium? It seems as if it would be way to wet for it at that point.


Attached is a pic of said dish.


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“And those who were seen dancing were thought to be insane by those who could not hear the music”
                -Friedrich Nietzsche


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InvisibleJosex
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Re: Agar Plate Condensation Dilemma [Re: 6Curi0us.M0nkey9]
    #27070373 - 12/03/20 05:00 PM (3 years, 1 month ago)

Condensation is not gonna harm anything but I can't see any condensation in the pic anyway.
Myc isn't gonna bust through the parafilm. You're overthinking this.


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OfflineMySillyUmRuns
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Re: Agar Plate Condensation Dilemma [Re: 6Curi0us.M0nkey9]
    #27070386 - 12/03/20 05:07 PM (3 years, 1 month ago)

I wouldn't worry about the condensation nor will the mycelium bust through the parafilm. If you want to slow it down a bit to buy some time, just place it in a ziplock in the refrigerator until you're ready to transfer it to more plates


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Offline6Curi0us.M0nkey9
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Re: Agar Plate Condensation Dilemma [Re: Josex]
    #27070400 - 12/03/20 05:18 PM (3 years, 1 month ago)

Thanks, I let it sit at room temp for awhile so I could take a good pic so no condensation in the pic. Sorry I'm out here noobin hardcore with the silly questions


--------------------
“And those who were seen dancing were thought to be insane by those who could not hear the music”
                -Friedrich Nietzsche


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Offline6Curi0us.M0nkey9
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I'm a teapot


Registered: 05/03/20
Posts: 51
Loc: Nirvana
Last seen: 2 years, 6 months
Re: Agar Plate Condensation Dilemma [Re: MySillyUmRuns]
    #27070402 - 12/03/20 05:19 PM (3 years, 1 month ago)

Thank you, ziploc bag is just a contam barrier or does it serve a different purpose?


--------------------
“And those who were seen dancing were thought to be insane by those who could not hear the music”
                -Friedrich Nietzsche


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