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The Fresh Prints
Smell ya later



Registered: 05/19/12
Posts: 1,377
Loc: Bel-Air
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: Ganodermalucidum] 2
#28069590 - 11/25/22 11:02 PM (1 year, 2 months ago) |
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I meant more water for cubes. I run my semps pretty dry. I have side-by-sides going and the wet bags are looking like shit . I'll post some results when they're finishing up
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CJD


Registered: 08/10/22
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Quote:
The Fresh Prints said:
https://www.shroomery.org/forums/showflat.php/Number/27971351
This pretty much sums up how I run bags for semps. Same for cubes but I use more water and a thicker top layer
Thanks for that. I need to do better at keeping up with what I'm wanting to try. I definitely read this further up this thread I believe. Thanks for sharing it again with me
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Ganodermalucidum
Pollinator of the Universe


Registered: 11/19/17
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: The Fresh Prints] 1
#28070014 - 11/26/22 11:27 AM (1 year, 2 months ago) |
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Got it Fresh Prints! That makes more sense. My slightly drier subs have performed much better than the wet ones as well. We all happily await pictures of your next efforts...even your ugly child wet bottom bois π€£.
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Moopers
Pan noob



Registered: 05/08/20
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Re: Official P. Subtropicalis/Semperviva Thread [Re: Moopers]
#28070107 - 11/26/22 12:38 PM (1 year, 2 months ago) |
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Quote:
Moopers said: My 2020 print from Alan R. hasn't germinated 18 days later, but no surprise there.
Below is a Xico (Veracruz, MΓ©xico) wild print germ plate, PDA + antibiotic. The myc cleaves closely to the surface of the agar, and isn't very organized. I was unsure that this is the myc I was looking for, but it took 7-8 days to germinate, is slow growing, and hasn't changed color or done anything else weird in 15 days. Either the myc is a slow contam or this is a very clean print. These pics are from day 11:


Transfers are looking more organized and fluffier, but still not as fluffy as other pics of the species on agar I've seen here. Transfers are to PDA agar, no antibiotic, which may be why the growth is more organized.

Thoughts? These look OK/like the real deal? Anyone else started a wild print of subtrop?
Waiting on germination from Melgo's print on PDA no AB, only been 3 days. Will be interesting to compare!
Update: growth on the germ plates of Melgo's print looks the same. Must be these pre-poured agar plates I bought from a different vendor.
I'd like to start pouring my own but don't have a flow hood. I saw SirPsycho recommended some autoclavable petri dishes. I might pick those up. Anyone have a tek for those, or would any no-pour tek be easily adapted to them?
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Rusty2096
rah rah raw in Lady gaga



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Re: Official P. Subtropicalis/Semperviva Thread [Re: nosf3r4tu] 1
#28070123 - 11/26/22 12:47 PM (1 year, 2 months ago) |
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-------------------- Currently looking for nothing. You guys who sent me stuff are straight up awesome!. We don't own things - things own us. Semi-solid liquid culture (SSLC)
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A.k.a
Stranger



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Re: Official P. Subtropicalis/Semperviva Thread [Re: Rusty2096] 1
#28070196 - 11/26/22 01:30 PM (1 year, 2 months ago) |
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Dude pouring plates is the easiest part of agar. If you can work transfers and jars in a SAB you can very easily pour plates.
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LAGM2020     
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0t0lerance


Registered: 05/01/21
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Re: Official P. Subtropicalis/Semperviva Thread [Re: Rusty2096] 1
#28070197 - 11/26/22 01:30 PM (1 year, 2 months ago) |
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Shrimps
Traveler



Registered: 10/13/22
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Re: Official P. Subtropicalis/Semperviva Thread [Re: 0t0lerance]
#28070200 - 11/26/22 01:31 PM (1 year, 2 months ago) |
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I've tried no pour. I just feel more comfortable pouring, got also less condensation that way ^^ Just go with what you feel more comfortable ;-) Both are great methods!
-------------------- I am not crazy, I prefer the term mentally hillarious. Agar - The way to go! Clean spawn checklist Proper Surface Moisture Recognizing and dealing with contamination π
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SirPsycho
Purple Belt in Google-Fu



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Re: Official P. Subtropicalis/Semperviva Thread [Re: Shrimps] 5
#28070417 - 11/26/22 04:10 PM (1 year, 2 months ago) |
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Hopefully this 2nd flush makes it to maturity. All my first flushes aborted late into growth, way past this point so fingers crossed these don't do the same
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Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
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    "Mist your balls and fan your asshole" - Pandaskis, 2023
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Moopers
Pan noob



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Re: Official P. Subtropicalis/Semperviva Thread [Re: SirPsycho]
#28070669 - 11/26/22 07:30 PM (1 year, 2 months ago) |
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Thanks, all. I'm going to try the no-pour petris, and if that doesn't work out, I'll try pouring in my SAB. Most advice I've seen on agar is "don't bother pouring without a flow hood cuz you'll just get contam", so I've stayed away from it.
I still have like 15 of these PDA plates to go through first, tho.
Now to pick a method (or two) for growing Subtrops. I have bags and can easily do them like The Fresh Prints, also have some 6qt shoeboxes, 30L unmodified monos, and one 30L modded with two sets of 1.25" holes. I have a JCM, but it'll be occupied with pans for a bit.
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Ganodermalucidum
Pollinator of the Universe


Registered: 11/19/17
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Re: Official P. Subtropicalis/Semperviva Thread [Re: Ganodermalucidum] 4
#28070702 - 11/26/22 08:06 PM (1 year, 2 months ago) |
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Here's my tub that had a wetter substrate than my others. Way squatter fruits and much slower to mature.
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0t0lerance


Registered: 05/01/21
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Quote:
Ganodermalucidum said: Here's my tub that had a wetter substrate than my others. Way squatter fruits and much slower to mature. 
This makes me wonder.. maybe the time it takes to maturity, could it be a factor in the strength of the final fruits? I really wonder what made 1-2gr a normal trip for one and 0.5 a fasten-your-seatbelts for another. Nevertheless nice tub ganoderma, very nice!
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Ganodermalucidum
Pollinator of the Universe


Registered: 11/19/17
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Re: Official P. Subtropicalis/Semperviva Thread [Re: 0t0lerance]
#28071014 - 11/27/22 01:24 AM (1 year, 2 months ago) |
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Thank you kindly. It certainly does seem that way. I've taken these once before (1.75 grams) and they certainly were much stronger than cubes, but not overwhelming like some people have described on much lower doses. Those were spawned very shortly after colonization. These however were left in the jars for 7 months to the point where they were pinning in the jars. Will report back on any difference in strength. Not going to lie though, I'm a little scared ππ°.
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goatboy666
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Does anyone know how long semp spores are roughly viable for? I had a print a few years back. I germed them no issue but things got hectic and i couldn't grow them out....
Around 8 months later, i gave them another try. Numerous trys. Absolutely no life in them.
Is this a common theme with these spores?
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Adas
Lonely Dreamer



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Re: Official P. Subtropicalis/Semperviva Thread [Re: goatboy666]
#28071086 - 11/27/22 05:17 AM (1 year, 2 months ago) |
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It's a common theme with any spores, pretty much. The mycelium stores WAY better. Especially Subtrop myc can survive a lot.
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0t0lerance


Registered: 05/01/21
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Quote:
Ganodermalucidum said: These however were left in the jars for 7 months to the point where they were pinning in the jars. Will report back on any difference in strength. Not going to lie though, I'm a little scared ππ°.
Yes, although I heard some say, despite higher doses, not to be intimidated by this specie due to it's friendly character (beta-carbolines?), so if it turns out way stronger than anticipated, I would keep that in the back of my mind. Safe journeys.
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0t0lerance


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Re: Official P. Subtropicalis/Semperviva Thread [Re: Adas] 1
#28071138 - 11/27/22 06:44 AM (1 year, 2 months ago) |
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Quote:
Adas said: It's a common theme with any spores, pretty much. The mycelium stores WAY better. Especially Subtrop myc can survive a lot.
Hm, yeah, last time I made some LC's so I used a few syringes on grain to see if they turned out clean.. either my grain was on the dry side.. or did something wrong with the LC.. myc looks weak; took way longer to show after an initial shake and after 2nd shake also an extended period to recover apart form that not all kernels seem to be colonized.. or just covered very lightly.. very thin no thick growth at all. Even injected more LC.. gave it a 3rd shake in the hope to finish the jars. These are from 9-nov.. this is 1 day after the 3rd shake:
Edited by 0t0lerance (11/27/22 06:45 AM)
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Shrimps
Traveler



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Re: Official P. Subtropicalis/Semperviva Thread [Re: 0t0lerance] 1
#28071143 - 11/27/22 06:50 AM (1 year, 2 months ago) |
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It looks for me badly contaminated Better wait what others have to say about it.
-------------------- I am not crazy, I prefer the term mentally hillarious. Agar - The way to go! Clean spawn checklist Proper Surface Moisture Recognizing and dealing with contamination π
π΄ π° πΌ π² π» πΈ π½ πΆ π
π
π° πΏ
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0t0lerance


Registered: 05/01/21
Posts: 962
Loc: The Netherlands
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Re: Official P. Subtropicalis/Semperviva Thread [Re: Shrimps]
#28071209 - 11/27/22 07:39 AM (1 year, 2 months ago) |
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Quote:
Shrimps said: It looks for me badly contaminated Better wait what others have to say about it.
The photo doesn't help here, but it's not contaminated, I can say that with 100% certainty.. this is just because I shook it yesterday.
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Melgo
Semperviva Fanatic



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Re: Official P. Subtropicalis/Semperviva Thread [Re: 0t0lerance] 14
#28073055 - 11/28/22 01:48 PM (1 year, 1 month ago) |
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These photos are dedicated to Hindsight 
We've been exchanging PMs and he gave me great advises. They've been put to practice and voilΓ ... my best Semperviva grow ever:



(The fruits are actually darker than the photos show).
This is a dub-tub shoebox. It's a Semp clone. 100% coir, cased with cactus soil at 100% colonization. Coir & soil where squeezed as hard as possible before being used. Pins appeared 14 days after spawning. These fruits are closed to 3 weeks old, not dropping spores yet.
Here are the three main points Hindsight gave me that changed everything for my Semp grows (where before I'd get 90% failure rate - by the Green Monster):
- Pasteurizing stuff for 2 hours (instead of one).
- Spawning in a different room than the one I was using before.
- Covering all my grains with a thin layer when spawning (even if a casing is applied afterward).
I've got 5 more various containers like this (shoeboxes, pasty EZD, buckets), in a month I'll be drowning in Semperviva 
Thanks a lot Hindsight 
What's super interesting too is that this clone in my automated FC where lighting and FAE are optimal grows nice and straight. Whereas here, the shoebox is on top of a wardrobe, and far for the window, I get spaghetti-like fruits. This time caps are developing nicely because I manually fan these fruits twice a day.
Here is a photo of this clone grown in the automated FC:

To conclude, with my previous way of growing mushrooms I'd get 10% success with Semp, 80% success with Mex. A, and 95% success with Natalensis. Which to me says a lot about how Natalensis is such an incredibly hardy mushroom! And thank God for that, because it helped me keep my sanity while trying to debug my Semp (many) failures.
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