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coAsTal
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Ambrose

Registered: 01/16/20
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Re: Official P. Subtropicalis/Semperviva Thread [Re: coAsTal]
#27199984 - 02/11/21 06:32 PM (2 years, 11 months ago) |
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Nice, both from the same fruit?
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mycorry
The Empirical



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Re: Official P. Subtropicalis/Semperviva Thread [Re: coAsTal] 1
#27199995 - 02/11/21 06:44 PM (2 years, 11 months ago) |
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Quote:
coAsTal said:
 

 Oh man, I haven't seen a clean cloning in a while!
Looks good!
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coAsTal
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Re: Official P. Subtropicalis/Semperviva Thread [Re: Ambrose]
#27200062 - 02/11/21 07:05 PM (2 years, 11 months ago) |
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Quote:
Ambrose said:

Nice, both from the same fruit?
Yes, the philosophy is "Two is One, One is None"
Taken from this big girl-- this is unfortunately the best pic I got before cutting. Had the meatiest stipe and tied for nicest cap size and pheno I like

Thank you mycorry-- it's my first clone-- hoping for beginners luck
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nosf3r4tu


Registered: 03/26/19
Posts: 775
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Re: Official P. Subtropicalis/Semperviva Thread [Re: Ambrose]
#27200719 - 02/12/21 12:53 AM (2 years, 11 months ago) |
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Quote:
Ambrose said: Double post but I followed the advice about dunking(I forget who recommended it):

Seems to have worked well. I just poured cold water on top and then dumped it off without recasing, this flush is looking much better than the first which only yielded a few lone fruit. So whoever gave that advice, thank you!
Also one thing that sucks about having these in fruiting for so long is the possibility of fungus gnats, first time I’ve seen these fuckers in awhile but they’re all up in the casing, ugh.
For me changing the casing did it. They wouldn't even touch it. I took it off scraped the myc a bit and put some verm on top. Now I have pins. So happy.
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Tweeq
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Re: Official P. Subtropicalis/Semperviva Thread [Re: nosf3r4tu]
#27200752 - 02/12/21 01:49 AM (2 years, 11 months ago) |
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Do you guys wait with casing untill 100 pct colonized? I have three trays of these colonizing rn @ room temp. Peeked into ibe last night and it looks like it can go for a bit longer before case and fruit. They are now six days from spawn.
I'll update with pics
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nosf3r4tu


Registered: 03/26/19
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Re: Official P. Subtropicalis/Semperviva Thread [Re: Tweeq]
#27200777 - 02/12/21 02:12 AM (2 years, 11 months ago) |
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I think you can give them a few more days. Colonization speed seem to be similar to cubes. But the fruiting... It's been a week since I saw knots forming and they're just small pins now.
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Tweeq
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Re: Official P. Subtropicalis/Semperviva Thread [Re: nosf3r4tu]
#27200868 - 02/12/21 04:38 AM (2 years, 11 months ago) |
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Think I'll wait untill the surface looks all white and then case & fruit.
Sub is experimental (for me at least): For my three trays I used one 650 gram coco coir brick, about 1 liter of verm, a few handfuls of garden compost, a handful of straw, a handful of sand and some garden lime, pasteurized and divided over three separate 6 liter trays, not completely full. One myco liter of spawn per tray. Probably comes down to 20 pct spawn. Maybe a bit much but we'll see.

Will be cased with pasteurized coir verm sand
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mycorry
The Empirical



Registered: 06/01/18
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Re: Official P. Subtropicalis/Semperviva Thread [Re: Tweeq]
#27201262 - 02/12/21 10:26 AM (2 years, 11 months ago) |
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Quote:
Tweeq said: Do you guys wait with casing untill 100 pct colonized? I have three trays of these colonizing rn @ room temp. Peeked into ibe last night and it looks like it can go for a bit longer before case and fruit. They are now six days from spawn.
I'll update with pics
With subtrops, my main failures have come from casing before colonization has finished. I don't know why but that's the pattern I have seen. However, this phenomena could just be caused by cultural practices so don't think that "Casing early=bad" as set in stone.
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Tweeq
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Re: Official P. Subtropicalis/Semperviva Thread [Re: mycorry]
#27201299 - 02/12/21 10:50 AM (2 years, 11 months ago) |
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Quote:
mycorry said:
Quote:
Tweeq said: Do you guys wait with casing untill 100 pct colonized? I have three trays of these colonizing rn @ room temp. Peeked into ibe last night and it looks like it can go for a bit longer before case and fruit. They are now six days from spawn.
I'll update with pics
With subtrops, my main failures have come from casing before colonization has finished. I don't know why but that's the pattern I have seen. However, this phenomena could just be caused by cultural practices so don't think that "Casing early=bad" as set in stone.
Still I will take this as 'why would I not wait a few days longer' You are growing these with success. I'm just trying. Be great if something cloneable comes of it.
I'll stick with my initial plan to wait untill I see nothing but colonized substrate, before I case. Thanks @mycorry for the response.
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Crackatoa
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Re: Official P. Subtropicalis/Semperviva Thread [Re: Tweeq] 1
#27201388 - 02/12/21 11:31 AM (2 years, 11 months ago) |
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I'm gonna have to put the viva to agar.
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Nichrome
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Re: Official P. Subtropicalis/Semperviva Thread [Re: Crackatoa]
#27201669 - 02/12/21 01:22 PM (2 years, 11 months ago) |
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Got a bunch of nice different sectors running away from each other. You guys looking for the fluffy stuff or the aggressive running type stuff with these?
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mycorry
The Empirical



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Re: Official P. Subtropicalis/Semperviva Thread [Re: Nichrome] 1
#27201699 - 02/12/21 01:33 PM (2 years, 11 months ago) |
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Quote:
Nichrome said: Got a bunch of nice different sectors running away from each other. You guys looking for the fluffy stuff or the aggressive running type stuff with these?
Good question. 
There hasn't really been a consensus on what the most ideal myc looks like, even with regards to myc growth speed. I would suggest taking the two or three most unique looking/performing myc and fruit them separately to find out. Then, come show us what you figured out.
This may be a fairly common species for an exotic, but there is still a lot of demystifying to do.
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mycorry
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Re: Official P. Subtropicalis/Semperviva Thread [Re: coAsTal] 1
#27201724 - 02/12/21 01:47 PM (2 years, 11 months ago) |
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Quote:
coAsTal said:
Thank you mycorry-- it's my first clone-- hoping for beginners luck
Good luck! 
Keep in mind that there isn't yet a consensus on what is the best way to clone subtrops, or how best to select them. That is for all of us to figure out together.
So far I have had decent luck with: Dish pins First fruiting clusters odd looking but vigerous fruit Anything that grew well in my specific environment Something that killed and ate a mold before fruiting(this is always a fun one)
I usually clone from inside the bottom of the stipe, near the casing. This is due to an old quote from RR about the bottom of the stipe being where most of the growth is happening(for cubes). I do this to try to capture the momentum, so to speak. Does it make a difference? I don't know yet. I have tried cap cloning caerulescens and zaps with decent results, but not a subtrop.
Basically what I am trying to say to everyone is: Write down all the details about what you do(with photos if possible) and post your results here to add to the knowledge we are building. We need more info.
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Tweeq
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Re: Official P. Subtropicalis/Semperviva Thread [Re: mycorry]
#27207266 - 02/15/21 11:31 AM (2 years, 11 months ago) |
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Well I cased all three trays today (top layered is more accurate)

After casing, in fc:
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mycorry
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Re: Official P. Subtropicalis/Semperviva Thread [Re: Tweeq]
#27207324 - 02/15/21 12:17 PM (2 years, 11 months ago) |
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Quote:
Tweeq said: Well I cased all three trays today (top layered is more accurate)

After casing, in fc: 
I just cased 6 tubs the same day as you with several different clone cultures. I'm doing Pasty EZ dial mono style with peat/verm casing. The myc has been colonizing the substrate for three weeks and it looked like it had finished five or so days ago. It's my first time using this tub style and my first time using grain in a while. I'm hoping to keep these alive long enough to potentially side by side.
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Tweeq
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Re: Official P. Subtropicalis/Semperviva Thread [Re: mycorry]
#27207346 - 02/15/21 12:29 PM (2 years, 11 months ago) |
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I'm seeing a lot of different spawn ratios with this species. From 1:10 (on the other website) all the way up to 1:2. I went with 1:5.
Seems all over the place. Any thoughts on this, anyone?
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nosf3r4tu


Registered: 03/26/19
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Re: Official P. Subtropicalis/Semperviva Thread [Re: Tweeq]
#27207623 - 02/15/21 02:43 PM (2 years, 11 months ago) |
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My grow (400ml container) is 1 part grains, 1 part straw, 1 part manure. Inoculated with Ms agar wedge (inoculation plate) . I had 3 but 2 got contam and threw them out eventually. The biggest pin is about 1" and I have a few clusters in there. Can't wait to clone and start something bigger.
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cruisinalltheway
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Re: Official P. Subtropicalis/Semperviva Thread [Re: nosf3r4tu]
#27208262 - 02/15/21 08:32 PM (2 years, 11 months ago) |
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anyone have success getting prints ? any tips on getting them to drop?
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Ambrose

Registered: 01/16/20
Posts: 518
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Re: Official P. Subtropicalis/Semperviva Thread [Re: cruisinalltheway] 1
#27212666 - 02/18/21 09:29 AM (2 years, 11 months ago) |
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Dunked tray that's been kept in a pasty ez dial mono
Edited by Ambrose (02/18/21 09:45 AM)
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