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OfflineMelgo
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: Adas] * 1
    #28010096 - 10/21/22 11:23 PM (1 year, 3 months ago)

Quote:

Adas said:
Melgo and Fresh killing it again! :rockon:




Thank you for the kind word Adas, but I feel more like I'm limping along with sometimes some lucky grows to show. Because I can tell you Mate, I still have a fuckton of contamination :lol: But I keep working at it, and one day...

The Fresh Prints IS killing it! So impressive, so inspirational :yesnod:

Totally agree with The Fresh Prints on the coir prep, I've always prepped it on the dry side. Great log btw :thumbup:


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Invisiblekirkeng
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: Melgo] * 13
    #28010407 - 10/22/22 09:06 AM (1 year, 3 months ago)

One week since pins first appeared, pictured is the best tub so far. Very happy with the pin set on this one, will definitely get some good clone donors. Other two tubs are more lack luster, one is still kicking good growth though much less coverage and more sporadic, the third as I had thought before definitely seems to be stalled out. I will continue waiting and perhaps clear it and try again. Treating them the same as I do for tamps and save for that one tub they seem to be responding well. Decided to restrict air flow on the less dense tub to compare development, if it ends up being worse I won’t be as disappointed in that loss, not fucking with the good ones though lest they get mad at me.
Also a question for ya’ll, how well are cultures keeping for people? They’re giving me the impression they’re resilient and would keep very well like tampanensis but any input appreciated as I start planning ahead and integrating into the rotation.


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OfflineMelgo
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: kirkeng] * 1
    #28010542 - 10/22/22 11:20 AM (1 year, 3 months ago)

What an amazing pinset! :rockon:


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InvisibleThe Fresh PrintsS
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: Melgo]
    #28010552 - 10/22/22 11:29 AM (1 year, 3 months ago)

Quote:

Melgo said:
What an amazing pinset! :rockon:



:whathesaid:


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Offline0t0lerance
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: The Fresh Prints] * 7
    #28010594 - 10/22/22 12:02 PM (1 year, 3 months ago)

Storing some subtropicalis LC's in the fridge..


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OfflineRotnpins
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: nosf3r4tu] * 9
    #28010627 - 10/22/22 12:18 PM (1 year, 3 months ago)

First Semperviva pins coming in. Spawned 9/25


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OfflineCJD
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: Rotnpins] * 1
    #28011201 - 10/22/22 07:09 PM (1 year, 3 months ago)

Quote:

Rotnpins said:
First Semperviva pins coming in. Spawned 9/25




:rockman:
Excited to see this play out. I feel like I've been waiting months cheering this grow on :lol:


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OfflineRotnpins
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: CJD] * 1
    #28011250 - 10/22/22 07:42 PM (1 year, 3 months ago)

Quote:

CJD said:
Quote:

Rotnpins said:
First Semperviva pins coming in. Spawned 9/25




:rockman:
Excited to see this play out. I feel like I've been waiting months cheering this grow on :lol:




Thanks.. hopefully it doesn't disappoint.

I've already got a couple clusters of side pins going... not sure if I can do anything to help the surface conditions tho.. I think there was a post just a couple days ago saying that they abort really easily or something if you mist once it's pinning.. :shrug:


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OfflineCJD
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: Rotnpins]
    #28011345 - 10/22/22 08:41 PM (1 year, 3 months ago)

Just T1-ed from my 2 germ plates.  The germ plates looked great. I struggled getting the transfer off the scalpel in the condiment cups so hopefully I didn't add any contams in the process. Need to figure out a better process for placing the transfer more easily.



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OfflineRusty2096
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: CJD] * 1
    #28011350 - 10/22/22 08:44 PM (1 year, 3 months ago)

I don't use condiment cups but I can see the problem.

Make your cuts and then use tweezers maybe?


--------------------
Currently looking for nothing. You guys who sent me stuff are straight up awesome!. :mushroom2:

We don't own things - things own us.

Semi-solid liquid culture (SSLC)


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OfflineCJD
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: Rusty2096]
    #28011362 - 10/22/22 08:52 PM (1 year, 3 months ago)

Quote:

Rusty2096 said:
I don't use condiment cups but I can see the problem.

Make your cuts and then use tweezers maybe?




Yeah I started with petris & they're fantastic but I burned through 80 in no time flat.  I really hate wasting that much plastic so I opted for no pours in the pp bead containers & wasn't a fan.  So now I'm testing out the condiment cups & seeing the issues with them as well.  I may just have to swallow my "earth friendly" ego & make a concession with petris 500 at a time.


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Invisiblekirkeng
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: Rotnpins] * 2
    #28011363 - 10/22/22 08:52 PM (1 year, 3 months ago)




I think there was a post just a couple days ago saying that they abort really easily or something if you mist once it's pinning.. :shrug:



Can’t speak to the post you read earlier and I’m still fresh into these guys but I will say the tub I posted above has been getting misted very heavily twice a day since pins and no aborts present, take it with a grain of salt though, I dunno if I’m lucky or not with no other grows under my belt.


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OfflineRotnpins
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: kirkeng]
    #28011589 - 10/22/22 10:17 PM (1 year, 3 months ago)

Quote:

kirkeng said:



I think there was a post just a couple days ago saying that they abort really easily or something if you mist once it's pinning.. :shrug:



Can’t speak to the post you read earlier and I’m still fresh into these guys but I will say the tub I posted above has been getting misted very heavily twice a day since pins and no aborts present, take it with a grain of salt though, I dunno if I’m lucky or not with no other grows under my belt.</font></font>




That's good news :super: maybe 1 or 2 other people can confirm this? If so, I'll start misting in the morning.. it could definitely use it if the substrate is already shrinking in enough for some side pins.


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OfflineCJD
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: Melgo] * 1
    #28011593 - 10/22/22 10:22 PM (1 year, 3 months ago)

I thought I remembered someone saying they misted to keep the caps from curling but wasn't sure about during pining.

Here's the link to the post below

Quote:

Melgo said:
Quote:

Konbri said:
Melgo, what a nice canopy in the jar!
What's your casing mix, and what's the drill, case->spray some water now and then, or just wait?
Thanks




Thanks! It's a commercial cactus soil mix. Nothing fancy apart from the fact that there is no HPOO in this one. I could take a photo of the bag if you want the composition.

Drill is: Case. Lightly mist once a day. When pins appear, mist once a day but a bit more. When mushrooms are nicely developed mist heavy-ish twice a day so that caps don't curl up and spores drop quickly and heavily.

Edit: this what I do for printing Semp.




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OfflineRotnpins
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: smalltalk_canceled]
    #28011599 - 10/22/22 10:30 PM (1 year, 3 months ago)

I found the post I was referring to.. I just skimmed it the first time, so my memory wasn't the best. It wasn't talking about aborts, rather contam.. so idk. I'm still open to the idea of misting, or even a quick bottom watering, but I was wondering if anyone had thoughts about this?  So far this is the only post I've found that suggests it might be better to wait.

Quote:

smalltalk_canceled said:
letting them dry out more rather than keeping conditions up like cubes, have induced fruiting in around 3 small subs. Excited to see development. fan of the drying out more than cubes theory

coir was brow-dry but white primordia was forming - the question is what to do after? im afraid to mist, thinking it will contam. ive seen my sluggish subtrops contam a lot at the primordia stage when you mist them 
maybe thats a exact point to avoid - misting at the primorida stage.





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InvisibleThe Fresh PrintsS
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: Rotnpins] * 1
    #28011745 - 10/23/22 12:03 AM (1 year, 3 months ago)

Quote:

Rotnpins said:


Thanks.. hopefully it doesn't disappoint.

I've already got a couple clusters of side pins going... not sure if I can do anything to help the surface conditions tho.. I think there was a post just a couple days ago saying that they abort really easily or something if you mist once it's pinning.. :shrug:





For what it's worth, I always see knots on the sides of my bags before I see them on the surface, but the side pins never seem to develop :shrug:



If this is your first run with semps, I'd suggest keeping it as simple as possible. Misting shouldnt hurt anything, but I'd just let it ride as is, personally :thumbup:

Looking good btw :awesomenod:


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Offline0t0lerance
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: CJD] * 1
    #28011749 - 10/23/22 12:07 AM (1 year, 3 months ago)

Quote:

CJD said:
Quote:

Rusty2096 said:
I don't use condiment cups but I can see the problem.

Make your cuts and then use tweezers maybe?




Yeah I started with petris & they're fantastic but I burned through 80 in no time flat.  I really hate wasting that much plastic so I opted for no pours in the pp bead containers & wasn't a fan.  So now I'm testing out the condiment cups & seeing the issues with them as well.  I may just have to swallow my "earth friendly" ego & make a concession with petris 500 at a time.




Checkout https://www.shroomery.org/forums/showflat.php/Number/26374264
Aliens holy agar containers, unless that is what you mean with 'bead containers'. I have just 18 of these containers and love them.. just whip up some LMEA agar (I like to save any liquid from boiling potatoes/grain, store in the fridge and add that too). Pc for 20min.. get m out hot and put an oven slide on top and pour out the hot water from the pc.. after cooling down ready yo use. I really love them, the fresher the better, I use them as soon as they cool, straight out of the pc never noticed a contamination problem, never, even if there is still loads condensation on the sides and lid.. some warped because they weren't loose enough in the PC when I started with them, but they are still good to use, even warped. But yeah I do this small scale.. 1 or 2 species at a time.. these things are too expensive for loads of agar work. They are not 100% transparent.. to be honest it never bothered me.

Fiddling with agar happens.. I tried making every thin agar once, horrible, flimsy, had to use the knife as a spatula. But yeah, after some fiddling you'll get better at it, if your sterile technique is ok, you don't have to worry if it takes a few seconds longer as long as you don't make hasty movements that could agitate mold spores..


Edited by 0t0lerance (10/23/22 12:14 AM)


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OfflineRotnpins
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: The Fresh Prints]
    #28011766 - 10/23/22 12:16 AM (1 year, 3 months ago)

Quote:

The Fresh Prints said:
Quote:

Rotnpins said:


Thanks.. hopefully it doesn't disappoint.

I've already got a couple clusters of side pins going... not sure if I can do anything to help the surface conditions tho.. I think there was a post just a couple days ago saying that they abort really easily or something if you mist once it's pinning.. :shrug:





For what it's worth, I always see knots on the sides of my bags before I see them on the surface, but the side pins never seem to develop :shrug:



If this is your first run with semps, I'd suggest keeping it as simple as possible. Misting shouldnt hurt anything, but I'd just let it ride as is, personally :thumbup:

Looking good btw :awesomenod:




Thanks for the reply :super: I'll give it a mist in the morning.. and I'll snap a picture of the side pins.. I noticed like 2 clusters and a few random pins.. they're still small, but they're past the knotting stage

Yup, this is my first semp grow. Definitely keeping it simple.. pretty much running it exactly like a standard cube grow. Only things I did differently than a standard shoebox grow is went a little bit on the wet side of field capacity, and I've got slightly higher FAE (I'm currently using modded shoeboxes), but this is the same way I've been doing cubes lately.


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OfflineA.k.aM
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: Rotnpins] * 2
    #28011975 - 10/23/22 05:39 AM (1 year, 3 months ago)

The condiment cups are good for final transfers and then knocking up jars, but aside from that they were too small to really work a culture. When you pick up a wedge it’s easiest to just barely get it on the scalpel so it comes off easily in the cup. I just usually cut into the agar and drag the blade away and the wedge slides off the blade.



So I finally tried some of these. Like 3-4 months ago I ground a bunch up into capsules for a camping trip that never happened. They jsut sat in a bag on my shelf this entire time so I’m not sure if they were full strength. I took about .6 and it was decent. Very hard to compare to cubes. I had some of that psychedelic body buzz energy going on but no OEV and my mind was totally clear. However if I closed my eyes within maybe 5 seconds I’d be immersed in neon light visuals.

I had eaten quite a bit an hour before and it only lasted maybe 2-3 hours. Next time I think I’ll take twice as much and it should hit the spot. I still need to try the ones that have been stored in a jar though to see if the caps lost potency.


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LAGM2020


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OfflineCJD
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: 0t0lerance]
    #28012002 - 10/23/22 06:34 AM (1 year, 3 months ago)

The alien was the one I tried but a lot of the containers deformed & they are really hard to see through.  I just worked on 14 plates last night so currently I'd need more than the 20 no pours I bought. There were some better containers I finally found so we'll see how they perform Alien style. I'll give it another shot.


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