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ReverendMyc

Registered: 03/29/19
Posts: 1,495
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Re: Official P. Subtropicales/Semperviva Thread [Re: Melgo]
#27808545 - 06/06/22 03:39 PM (1 year, 7 months ago) |
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Well, poop.
 Day 26 since spawn, 16 since casing.
One day, my little pretty. One day!
-------------------- LAGM 2.024Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any moreHow to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice. Don't Panic   
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SirPsycho
Purple Belt in Google-Fu



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Re: Official P. Subtropicales/Semperviva Thread [Re: ReverendMyc]
#27808560 - 06/06/22 03:59 PM (1 year, 7 months ago) |
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Quote:
ReverendMyc said: Well, poop.
 Day 26 since spawn, 16 since casing.
One day, my little pretty. One day!
Ah, the Reddit special.
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Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖
    "Mist your balls and fan your asshole" - Pandaskis, 2023
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ReverendMyc

Registered: 03/29/19
Posts: 1,495
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Re: Official P. Subtropicales/Semperviva Thread [Re: SirPsycho] 2
#27808568 - 06/06/22 04:15 PM (1 year, 7 months ago) |
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Quote:
SirPsycho said: Ah, the Reddit special.
RIGHT?!?!
So you are saying it looks good and let it ride. ya?
-------------------- LAGM 2.024Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any moreHow to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice. Don't Panic   
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SirPsycho
Purple Belt in Google-Fu



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Re: Official P. Subtropicales/Semperviva Thread [Re: ReverendMyc] 3
#27808569 - 06/06/22 04:17 PM (1 year, 7 months ago) |
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Maybe spray some peroxide just to be safe
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Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖
    "Mist your balls and fan your asshole" - Pandaskis, 2023
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jomanda1990
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Re: Official P. Subtropicales/Semperviva Thread [Re: SirPsycho] 1
#27808579 - 06/06/22 04:37 PM (1 year, 7 months ago) |
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Don't forget trapping the contaminated area under a glass jar
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Melgo
Semperviva Fanatic



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Re: Official P. Subtropicales/Semperviva Thread [Re: jomanda1990]
#27812858 - 06/10/22 02:58 AM (1 year, 7 months ago) |
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So my shoebox which knotted 6 days after spawning :

Turned into this after casing :

This massive Trich spot appeared in less than 24 hours 
I suspect the contamination came from the casing. Not from the spawn or the CVG substrate.
My question is: how can I test this hypothesis? Any pointers you experimented guys could give me?
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Hindsight
Mad Scientist


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Re: Official P. Subtropicales/Semperviva Thread [Re: Melgo] 1
#27812964 - 06/10/22 06:11 AM (1 year, 7 months ago) |
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Quote:
Melgo said: So my shoebox which knotted 6 days after spawning :

Turned into this after casing :

This massive Trich spot appeared in less than 24 hours 
I suspect the contamination came from the casing. Not from the spawn or the CVG substrate.
My question is: how can I test this hypothesis? Any pointers you experimented guys could give me?
Have you ever had casing develop trich, just sitting around unused? Like if you pasteurize a bag of casing, have you ever just kept the bag closed or open on the shelf for days or weeks without getting around to using it? I have and never once has trich developed.
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A.k.a
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Re: Official P. Subtropicales/Semperviva Thread [Re: Hindsight] 1
#27812995 - 06/10/22 06:44 AM (1 year, 7 months ago) |
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Did it turn green?
It is really big but it doesn’t look like normal trich to me in that picture.
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LAGM2020     
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Melgo
Semperviva Fanatic



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Re: Official P. Subtropicales/Semperviva Thread [Re: Hindsight]
#27813002 - 06/10/22 06:52 AM (1 year, 7 months ago) |
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Quote:
Hindsight said:
Quote:
Melgo said: So my shoebox which knotted 6 days after spawning :

Turned into this after casing :

This massive Trich spot appeared in less than 24 hours 
I suspect the contamination came from the casing. Not from the spawn or the CVG substrate.
My question is: how can I test this hypothesis? Any pointers you experimented guys could give me?
Have you ever had casing develop trich, just sitting around unused? Like if you pasteurize a bag of casing, have you ever just kept the bag closed or open on the shelf for days or weeks without getting around to using it? I have and never once has trich developed.
Hey Hindsight, thanks a lot for chiming in.
No, I've never had any casing develop trich after sitting around. Max a casing sat around in a bucket for was probably a week tho' - I tend to use it quickly after pasteurizing it.
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Melgo
Semperviva Fanatic



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Re: Official P. Subtropicales/Semperviva Thread [Re: A.k.a]
#27813024 - 06/10/22 07:24 AM (1 year, 7 months ago) |
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Quote:
A.k.a said: Did it turn green?
It is really big but it doesn’t look like normal trich to me in that picture.
Yes it's turning green :/
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Hindsight
Mad Scientist


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Re: Official P. Subtropicales/Semperviva Thread [Re: Melgo] 4
#27813130 - 06/10/22 08:51 AM (1 year, 7 months ago) |
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Quote:
Melgo said: No, I've never had any casing develop trich after sitting around. Max a casing sat around in a bucket for was probably a week tho' - I tend to use it quickly after pasteurizing it.
This is why I usually don't blame substrates or casings.
The common belief around here seems to be that once your spawn is fully colonized by mycelium, and if it is a good strong culture, it will be able to defend against any trich introduced to it from the casing or sub. That's part of the reason everyone spawns to bulk in open air and doesn't use sterile procedures for it. Your tub is effectively in open air from this point forward so trich spores will be getting in there and trich spores are everywhere.
The other concern is if you have any uncolonized or partially colonized grains in your spawn (uncolonized because some grains are bacterial or simply because you didn't let the jar get to a true 100% colonization). Those uncolonized gains are open-season for trich to feed on.
Would love someone to correct me if any of the above is inaccurate but from everything I have experienced myself and read, this seems to be the current consensus. There may be a point to saying that if a substrate or casing material contains a particularly high load of trich spores, then this might factor into your grain's ability to defend against it. Pasteurizing your sub/casing should eliminate all the trich anyway so short of a failed pasteurization attempt, this shouldn't be an issue.
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MysticMycologist
Dirt Sherpa



Registered: 10/14/21
Posts: 1,755
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Re: Official P. Subtropicales/Semperviva Thread [Re: Hindsight] 4
#27813179 - 06/10/22 09:46 AM (1 year, 7 months ago) |
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My experience supports this. I recently used bucket tek CV that had been sitting in a bin for over a month in 4 shoeboxes. One shoebox developed trich, but it was the one with sketchy spawn that I suspected was slightly bacterial. The other three ran as normal.
I cult in a damp basement that likely has a huge spore load. I only ever have mold problems on grows that I use less than anexic spawn in. If my grain spawn looks even slightly bacterial, I almost always get trich or another mold before or just after the first flush. Tubs using clean spawn seem to always persist through multiple flushes before succumbing. I swear, since I have learned how to identify and make anexic spawn, it seems like I can get away with so much.
-------------------- Two eyes to look, One eye to see. Prying open my third eye 
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SirPsycho
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Re: Official P. Subtropicales/Semperviva Thread [Re: MysticMycologist] 1
#27813194 - 06/10/22 09:57 AM (1 year, 7 months ago) |
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As I understand it, Trichoderma sp can act as mycoparasites and can also germinate on just coir (albeit slowly).
I haven't run into it indoors but I did get it in outdoor grows with unpasteurized potting soil. Now I can't say what it used to germinate. My spawn was clean (I know everyone that gets contam says this) and fully colonized so I really really doubt it came from that.
Two of the three cases I encountered, the trich was clearly eating the myc. In one of the two cases, the trich straight up ate my pan cyan fruit. I think it was starting to eat tampanensis fruit once as well but it tossed it.
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Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖
    "Mist your balls and fan your asshole" - Pandaskis, 2023
Edited by SirPsycho (06/10/22 09:57 AM)
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Nichrome
I'm a torso!


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Re: Official P. Subtropicales/Semperviva Thread [Re: SirPsycho] 13
#27813311 - 06/10/22 11:54 AM (1 year, 7 months ago) |
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Trich and other molds, critters, bacteria, chemicals, etc can very well be "living" in some form or another in sub or casing material. I have seen molds grow on just coir that was prepped hot and sealed, more than one time. Trich in general won't fruit on coir alone or most casing materials. It takes advantage of the nutrient made available by giving it access to grain or other nutrients (spawn). Trich is really an opportunivor and will eat what it needs to to survive. In nature trich rarely forms isolated fruiting masses and seems to mostly just do that when it is isolated and needs to escape back into the outside/forest where it's complete homeostatic biome exists. It is just trying to escape and it will shank whatever motherfucker it needs to to get out and be free.
There are options of how it happened. I mentioned chemicals earlier on because a lot of the time chemicals can weaken your colony in one way or another which allows the trich to flourish, some things even that may be commonly used in a lot of myco gardens like isopropyl or certain terpenes which can allow greater permeability of cell walls and such. It may have germinated from spores donated by the air to the surface of your colony which has defensive mechanisms which prevent it from really taking hold and it sits there as a baby doing not much. Adding a layer of moist substrate gives those little babies the chance to grab onto some water and it gives them a direction to grow (upward) that does not have all the checks and balances that a fully formed colony maintains so it throws out an emergency isolated fruiting mass and kicks out as much spore as possible.
I honestly love molds a lot. I grow a good number of them on purpose. There are likely thousands of varieties of trichoderma and trichoderma generally adapts to things very quickly as it has been a worldwide event in it's self for millions of years and every ecosystem is dependent on it in one way or another. I think that P. cubensis primarily and other Psilocybes, pans, gyms etc are showing us this wonderful mold on purpose in hopes that some of us will have the humble recognition of it's important roles and the advantages we might have as a world should we take those lessons and pieces of life and apply them with loving conscience. Some of us who are looking at these molds regularly, and maybe cursing their very existence as the bane of many efforts, have all the skills necessary to work with these molds in the most fantastic ways. We may also have the vision of what they are for and where to use them. Is it mind control or is it love?
A lot of things here that are called trichoderma are also not trichoderma but aspergillus or other green spored molds.
Mold is magic and why it is there is not cut and dry. Mold doesn't do "dry".
-------------------- “Better to be deprived of food for three days, than tea for one.”
Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson
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SirPsycho
Purple Belt in Google-Fu



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Re: Official P. Subtropicales/Semperviva Thread [Re: Nichrome]
#27813357 - 06/10/22 12:37 PM (1 year, 7 months ago) |
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That was a great read, Nichrome.
I'm right there with you on the love for molds, I've started culturing my contams(and got yelled at by a TC, IIRC it was mushboy) and putting them into slants for when I have the equipment and resources to do something with them. No trich yet but I got Penicillium roqueforti off some blue cheese, a Rhizophus and what might be a Geotrichum. Haven't done the same with bacteria but I've been considering it.
Also, my go to way to describe how aggressive trich can be is "it'll fall off in your crib, eat to myc, slap yo momma on her booty, kick your dog, fuck your bitch"
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Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖
    "Mist your balls and fan your asshole" - Pandaskis, 2023
Edited by SirPsycho (06/10/22 12:38 PM)
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Melgo
Semperviva Fanatic



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Re: Official P. Subtropicales/Semperviva Thread [Re: SirPsycho]
#27813373 - 06/10/22 12:54 PM (1 year, 7 months ago) |
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Thanks for your inputs guys. It gave me few things to think about in order to tighten my processes.
Edited by Melgo (06/10/22 12:54 PM)
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Hindsight
Mad Scientist


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Re: Official P. Subtropicales/Semperviva Thread [Re: Melgo]
#27813394 - 06/10/22 01:11 PM (1 year, 7 months ago) |
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Great post Nichrome!
I am curious for any practical advice you have on reducing the risk of trich or other contaminates aside from the usual: Clean spawn, strong culture, full colonization, pasteurized subs and casings? I did see your note about chemicals as well.
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shruglife
¯\_(ツ)_/¯
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Re: Official P. Subtropicales/Semperviva Thread [Re: Doctor Mario]
#27814611 - 06/11/22 12:45 PM (1 year, 7 months ago) |
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What kinds of temp ranges have folks been successful at? I noticed Dr Mario noted 77F in a previous post. I’m hoping somewhere around 70 might work.
I’m finally getting a couple pins. They seem to be aborting though. They look thin and scraggly compared to photos I’ve seen here. I’ll be patient maybe the next ones peeking up will be better.
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SirPsycho
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Re: Official P. Subtropicales/Semperviva Thread [Re: shruglife] 1
#27814635 - 06/11/22 01:14 PM (1 year, 7 months ago) |
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I got some cakes fruiting buried in a flower pot outdoors where temps have ranged from below 50 to over 90.
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Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖
    "Mist your balls and fan your asshole" - Pandaskis, 2023
Edited by SirPsycho (06/11/22 01:14 PM)
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SirPsycho
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Re: Official P. Subtropicales/Semperviva Thread [Re: SirPsycho] 9
#27814748 - 06/11/22 03:04 PM (1 year, 7 months ago) |
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Here's those outdoor viva I was talking about.
They are so freaking fraking short compared to indoors
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Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖
    "Mist your balls and fan your asshole" - Pandaskis, 2023
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