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Offlinehazyhorse
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Re: Official P. Subtropicales/Semperviva Thread [Re: SirPsycho]
    #27630439 - 01/24/22 01:54 AM (2 years, 4 days ago)

just posting to come back to this thread later! i got a print awhile ago off the marketplace & am super excited to try this variety. i was skimming over the posts & i'm glad to see so much success w it. i believe i have the hoog variety. it took forever to pin on agar but i'm glad i kept the plate. will be making t2 plates tonight


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Offlinehazyhorse
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Re: Official P. Subtropicales/Semperviva Thread [Re: SirPsycho]
    #27630451 - 01/24/22 02:08 AM (2 years, 4 days ago)

edit: accidental double post lol


--------------------
you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿


Edited by hazyhorse (01/31/22 11:26 PM)


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OfflineMelgo
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Re: Official P. Subtropicales/Semperviva Thread [Re: hazyhorse]
    #27630535 - 01/24/22 05:21 AM (2 years, 4 days ago)

Do you guys ever get rhizomorphic mycelium on your agar plates with this species?
Because after many transfers, my mycelium always looks wispy. So I'm wondering :confused2:


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InvisibleWall.E
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Re: Official P. Subtropicales/Semperviva Thread [Re: Melgo] * 3
    #27630541 - 01/24/22 05:35 AM (2 years, 4 days ago)

Rhizomorphs aren’t a tool in every fungi’s toolbox. Some species won’t throw rhizomorphs out ever. Never seen rhizo growth on pans, tamps or semps. Doesn’t mean they can’t go rhizo, but I’m assuming they probably prefer not to


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OfflineMelgo
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Re: Official P. Subtropicales/Semperviva Thread [Re: Wall.E]
    #27630548 - 01/24/22 05:47 AM (2 years, 4 days ago)

Quote:

Wall.E said:
Rhizomorphs aren’t a tool in every fungi’s toolbox. Some species won’t throw rhizomorphs out ever. Never seen rhizo growth on pans, tamps or semps. Doesn’t mean they can’t go rhizo, but I’m assuming they probably prefer not to



Thanks a lot for the reply Dude!
That's reassuring for me, as I'm new to this species.


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Offlinerockyfungus
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Re: Official P. Subtropicales/Semperviva Thread [Re: Melgo]
    #27630621 - 01/24/22 07:21 AM (2 years, 4 days ago)

Quote:

Melgo said:
Do you guys ever get rhizomorphic mycelium on your agar plates with this species?
Because after many transfers, my mycelium always looks wispy. So I'm wondering :confused2:



Is wispy the right word for semp myc? It seems rather tomentose for me. Pans are wispy to me.

My 3 bags smelled off and weren't moving after 2 months in fruiting chamber. Mixed 'em all in a cloth bag with some manure. Going to throw it in the garage and neglect it. It's gonna freeze.

Next bags about ready. I still want to neglect a bag for invitro fruits.
Tray or shoebox? Treat it like pans or cubes?


Edited by rockyfungus (01/24/22 10:05 AM)


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OfflineMelgo
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Re: Official P. Subtropicales/Semperviva Thread [Re: rockyfungus] * 1
    #27631033 - 01/24/22 01:50 PM (2 years, 4 days ago)

Quote:

rockyfungus said:
Is wispy the right word for semp myc? It seems rather tomentose for me. Pans are wispy to me.





I haven't grown Pans yet, so I can't compare. But yes, tomentose might be a better word.

Natalensis (and Cubensis) mycelium is so different from one agar plate to another. As for Semp: they all look the same to me. Hence my previous question.

I don't even get the growth rings I do with Tampanensis & Mexicana.

I'm choosing which agar plate to put on rye based on colonization speed, and that's about it. So far, I haven't had any agar pins after leaving T1&2 plates alone for ~2 months.

We'll see what the shoebox and the Pasty EZD will do in a few days - but I feel like I'm kinda blind testing mycelium with this species :lol:


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OfflineA.k.aM
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Re: Official P. Subtropicales/Semperviva Thread [Re: Melgo] * 8
    #27635136 - 01/27/22 12:35 PM (2 years, 1 day ago)

Should be a (1g)trip report in a day or two.



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Edited by A.k.a (01/27/22 12:35 PM)


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OfflineMelgo
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Re: Official P. Subtropicales/Semperviva Thread [Re: A.k.a]
    #27635199 - 01/27/22 01:24 PM (2 years, 1 day ago)

Quote:

A.k.a said:
Should be a (1g)trip report in a day or two.






Sweet! Looking forward to it :dancer:
Have a good one!


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Offlinesmalltalk_canceled
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Re: Official P. Subtropicales/Semperviva Thread [Re: Melgo]
    #27635233 - 01/27/22 01:57 PM (2 years, 1 day ago)

Some of them are just teen-agers!!:shocking:


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Re: Official P. Subtropicales/Semperviva Thread [Re: smalltalk_canceled] * 1
    #27635370 - 01/27/22 04:00 PM (2 years, 1 day ago)

Buddy gave me some Convexa LC, so put a bit on some plates to ensure it was clean. I got too excited though and just put the rest to grain at the same time. YOLO



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OfflineAdas
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Re: Official P. Subtropicales/Semperviva Thread [Re: E_Dubbya] * 5
    #27635866 - 01/28/22 03:36 AM (2 years, 18 hours ago)

My spores from Ambrose germed in less than 2 days! Absolute insanity. I'm finally in the game, guys! :rockon::rockon:


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OfflineHindsight
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Re: Official P. Subtropicales/Semperviva Thread [Re: Adas] * 2
    #27636007 - 01/28/22 07:51 AM (2 years, 14 hours ago)

I'm jealous Adas. My hail mary attempt of loading the spore print into a syringe, letting it soak, then knocking up BRF cakes has not worked so far either. I should have a print in my mailbox from Rocky that I'm picking up this weekend so hopefully I'll be about a week behind you. Good luck with your grow!


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Offlinerockyfungus
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Re: Official P. Subtropicales/Semperviva Thread [Re: Hindsight] * 1
    #27636025 - 01/28/22 08:21 AM (2 years, 13 hours ago)

Hopefully you can get that print to fruit. I may go back for the third time...


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Re: Official P. Subtropicales/Semperviva Thread [Re: rockyfungus]
    #27636041 - 01/28/22 08:43 AM (2 years, 13 hours ago)

I am beginning to suspect that the spores of Semp and Tamp varieties are more susceptible to aging than other spore prints.

I got good germination from all of the prints that Ambrose generously shared with me last year, but I can't get any of them to germ a year later. They were kept in a drawer right next to my own tamp prints and PE swabs that I had no trouble germing in the same sessions. The tamp prints that did work are the next generation from the prints from Ambrose, so fresher. About 4 months old versus 14 months.

I still have some living myc in jars and a tub from the first Semp germination, so I am going to try to take a bit back to agar and see if I can get started again from there.


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Edited by ReverendMyc (01/28/22 08:44 AM)


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OfflineHindsight
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Re: Official P. Subtropicales/Semperviva Thread [Re: rockyfungus]
    #27636057 - 01/28/22 09:01 AM (2 years, 13 hours ago)

Quote:

rockyfungus said:
Hopefully you can get that print to fruit.




I can't promise that, but I can promise at least some trich.


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OfflineAdas
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Re: Official P. Subtropicales/Semperviva Thread [Re: Hindsight] * 5
    #27636077 - 01/28/22 09:31 AM (2 years, 12 hours ago)

Some species definitely have a short shelf-life. But you can freeze prints and hopefully prolong their lifespan, and potentially reduce germination time. See my thread about freezing spores. I think these Subtrop spores could have frozen during transit (very likely), so maybe that's why they germinated so fast, similar to my Tamp experiment?

EDIT: Please guys, feel free to perform your own experiments with freezing spores and contribute to my thread so we can have the info in one place. As far as I know, I'm the first one to have described this phenomenon so let's do some science! :leocheers:


Edited by Adas (01/28/22 09:34 AM)


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OfflineAdas
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Re: Official P. Subtropicales/Semperviva Thread [Re: Adas] * 3
    #27640393 - 02/01/22 05:05 AM (1 year, 11 months ago)

Sub germ mycelium going NUTS! Ambrose will be proud :lol:



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OfflineHindsight
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Re: Official P. Subtropicales/Semperviva Thread [Re: Adas] * 1
    #27640456 - 02/01/22 06:53 AM (1 year, 11 months ago)

You finally got a print that germinates!


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Offlinerockyfungus
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Re: Official P. Subtropicales/Semperviva Thread [Re: Adas] * 1
    #27640490 - 02/01/22 07:19 AM (1 year, 11 months ago)

That's the stuff! I'm really digging how voracious it is.
I did a bag of coir and hay, demolished. Bag of manure/hay/coir/verm, demolished.

This time around I'm leaving one bag to do it's thing. Other bag made 1 shoebox, 3 trays, and a bag.
Was a bit lazy and just mixed CVG/spawn in a bag and called it good. Rest of trays/boxes were mixed with CVG, cased with CVG, or left alone to be cased with 50:50.


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