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Rumblestrip

Registered: 04/21/19
Posts: 258
Loc: Canada
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: the_chosen_one] 2
#28609101 - 01/04/24 04:43 AM (24 days, 18 hours ago) |
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Quote:
the_chosen_one said: Probably this one..
https://www.biorxiv.org/content/10.1101/2023.11.01.564784v1.full.pdf[/quoteQuote:
the_chosen_one said: Probably this one..
https://www.biorxiv.org/content/10.1101/2023.11.01.564784v1.full.pdf
I haven't had a chance to re read your link TCO but i believe that is the article.
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Yuggoth
Mi-Go Cultivator



Registered: 03/04/23
Posts: 493
Loc: Lost Carcosa
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Rumblestrip] 4
#28609478 - 01/04/24 01:18 PM (24 days, 9 hours ago) |
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I finally started semp germ plates. Now the waiting ... Well, that gives me time to review the thread and figure out how to actually grow 'em compared to cubes.
-------------------- We have not succeeded in answering all our problems. The answers we have found only serve to raise a whole set of new questions. In some ways we feel we are as confused as ever, but we believe we are confused on a higher level and about more important things. -- Earl C. Kelley Things I really wish I knew when I started // Vacuum sealer discussion thread // Shroomery gif zoo
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the_chosen_one
On the Darkslide


Registered: 09/11/06
Posts: 2,885
Loc: 1984
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Rumblestrip] 1
#28609482 - 01/04/24 01:27 PM (24 days, 9 hours ago) |
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It's a hell of a read. I haven't made it through the whole thing.
Saw some Bell Cap germination yesterday. A little bac on the side too, but I think it'll be fine with a few transfers.
-------------------- "Luck favors the observant." - Workman
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ReverendMyc

Registered: 03/29/19
Posts: 1,496
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: the_chosen_one] 9
#28609485 - 01/04/24 01:31 PM (24 days, 9 hours ago) |
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Cross from LAGM. I got some bell caps germing, too.
 Thanks RockinRobot
-------------------- LAGM 2.024Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any moreHow to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice. Don't Panic   
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Baba Yaga
♥ coir grower

Registered: 09/13/20
Posts: 3,957
Loc: Hyperspace Chicken Coop
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: ReverendMyc] 13
#28609589 - 01/04/24 03:43 PM (24 days, 7 hours ago) |
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Telomere
Unravelling


Registered: 12/26/22
Posts: 172
Last seen: 3 hours, 23 minutes
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Baba Yaga] 6
#28609610 - 01/04/24 03:58 PM (24 days, 7 hours ago) |
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Snap!
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RockinRobot
Stranger


Registered: 12/08/22
Posts: 863
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Telomere] 9
#28609694 - 01/04/24 05:08 PM (24 days, 6 hours ago) |
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Glad to see all the prints are nice and viable. Good luck to everyone that got them.
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Gleyck
littlestitious



Registered: 08/17/22
Posts: 1,037
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: RockinRobot] 1
#28609956 - 01/04/24 09:39 PM (24 days, 1 hour ago) |
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Dear farmers, help me understand, too green on true casings here: I thought the purpose of a casing layer was to provide a above-surface layer of extra-humid microclimate to induce pinning, but this culture of mine is just colonizing it like coco-coir it was... which seems to undermine the original intention of using a true casing in the first place :
do all the variety of casings get eaten up, or just by some cultures, or just cactus soil - the one I'm presently trying?
hope all those LAGM rockin's bell caps translate the phenotype  I'll be lurking the marketplace like Quasimodo
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chris77
Archaic Revivalist



Registered: 01/17/22
Posts: 1,868
Last seen: 6 hours, 48 minutes
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Gleyck] 2
#28610084 - 01/05/24 02:27 AM (23 days, 20 hours ago) |
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Quote:
Gleyck said: Dear farmers, help me understand, too green on true casings here: I thought the purpose of a casing layer was to provide a above-surface layer of extra-humid microclimate to induce pinning, but this culture of mine is just colonizing it like coco-coir it was... which seems to undermine the original intention of using a true casing in the first place :
do all the variety of casings get eaten up, or just by some cultures, or just cactus soil - the one I'm presently trying?
hope all those LAGM rockin's bell caps translate the phenotype  I'll be lurking the marketplace like Quasimodo

Could be that you cased too early. I wait for full colonisation and then at least another week. i case when i see first knots.
-------------------- the observer is the observed j. krishnamurti
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Melgo
Semperviva Fanatic



Registered: 02/11/16
Posts: 692
Loc: Western Europe
Last seen: 8 minutes, 50 seconds
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Gleyck] 5
#28610116 - 01/05/24 04:10 AM (23 days, 19 hours ago) |
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Quote:
Gleyck said: Dear farmers, help me understand, too green on true casings here: I thought the purpose of a casing layer was to provide a above-surface layer of extra-humid microclimate to induce pinning, but this culture of mine is just colonizing it like coco-coir it was... which seems to undermine the original intention of using a true casing in the first place :
do all the variety of casings get eaten up, or just by some cultures, or just cactus soil - the one I'm presently trying?
hope all those LAGM rockin's bell caps translate the phenotype  I'll be lurking the marketplace like Quasimodo

Hey man, if it's the print you got from me, don't worry about it. It'll colonize the cactus soil to a good amount and then pin.
When I was talking with Hindsight, he'd concluded that casings with low PH would get colonized. When he had a casing with a PH of ~8 it wouldn't get colonized.
For the first time I've pasteurized cactus soil adjusted with lime last night. My cactus soil PH is ~6.2, hopefully I'll have raised this batch closer to 8 and will be able to see for myself is I get casing colonization with this same culture.
I ran this culture with and without casing and it produced way better pinset and fruits than without. But other members never needed to case and had stellar results too.
Here is a side by side of non-cased vs cased, same LC, same innoc date etc.:
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Melgo] 6
#28610131 - 01/05/24 05:08 AM (23 days, 18 hours ago) |
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Yeah my first tub ate the casing too

--------------------
LAGM2020     
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 2 days, 11 hours
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: A.k.a] 1
#28610328 - 01/05/24 10:38 AM (23 days, 12 hours ago) |
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It's safe to say it's a ph thing. I've always used pelletized lime and gypsum which is insanely inconsistent and I'd never recommend, but sometimes it gets colonized sometimes it doesnt
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Bigworm



Registered: 03/19/22
Posts: 1,873
Loc: Murica
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: tryptkaloids] 13
#28610384 - 01/05/24 11:22 AM (23 days, 11 hours ago) |
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Cross posting from this thread. Subtropicalis(semperviva) Harvested 5-25-23 Dried in dehydrator 24hrs @ 165f. Stored in glass jar with desiccant and O2 absorber. Well mixed when put in storage and picked 1 gram random sample to grind up for the test. I weighed out .075g of the homogenized mix for the test. Test read in direct sunlight. Results
 I'm going with just under 18mg not quite 18 but a fair bit darker then 15mg, so I'll say around 17mg x 2, since I cut the required amount of material in half, for a total of 34mg/g.
Edited by Bigworm (01/05/24 11:24 AM)
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Telomere
Unravelling


Registered: 12/26/22
Posts: 172
Last seen: 3 hours, 23 minutes
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Bigworm] 15
#28610461 - 01/05/24 12:24 PM (23 days, 10 hours ago) |
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Dinner is served
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chris77
Archaic Revivalist



Registered: 01/17/22
Posts: 1,868
Last seen: 6 hours, 48 minutes
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Bigworm] 4
#28610484 - 01/05/24 12:45 PM (23 days, 10 hours ago) |
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Quote:
Bigworm said: Cross posting from this thread. Subtropicalis(semperviva) Harvested 5-25-23 Dried in dehydrator 24hrs @ 165f. Stored in glass jar with desiccant and O2 absorber. Well mixed when put in storage and picked 1 gram random sample to grind up for the test. I weighed out .075g of the homogenized mix for the test. Test read in direct sunlight. Results
 I'm going with just under 18mg not quite 18 but a fair bit darker then 15mg, so I'll say around 17mg x 2, since I cut the required amount of material in half, for a total of 34mg/g.
Halleluja, fits my experience aswell. Gave 2g to my friends, they split it in 4 parts, properly made tea from a quarter, and strained it into two cups. She got really off on it and even he had the wooden floor move and the walls breathe… These mushrooms pack a punch for sure! 0.25 is a microdose with cubensis
-------------------- the observer is the observed j. krishnamurti
Edited by chris77 (01/05/24 01:56 PM)
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chris77
Archaic Revivalist



Registered: 01/17/22
Posts: 1,868
Last seen: 6 hours, 48 minutes
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: chris77] 11
#28611189 - 01/06/24 04:04 AM (22 days, 19 hours ago) |
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Not beautiful by any means, but hey, its the fourth flush from this topfruited quart jar. 45g fresh nevertheless, this seems a good way to have fresh semperviva around. Will give them another generous misting and a fresh bag.

-------------------- the observer is the observed j. krishnamurti
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Gleyck
littlestitious



Registered: 08/17/22
Posts: 1,037
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Melgo] 2
#28611787 - 01/06/24 01:59 PM (22 days, 9 hours ago) |
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Quote:
Melgo said: Hey man, if it's the print you got from me, don't worry about it. It'll colonize the cactus soil to a good amount and then pin.
When I was talking with Hindsight, he'd concluded that casings with low PH would get colonized. When he had a casing with a PH of ~8 it wouldn't get colonized.
For the first time I've pasteurized cactus soil adjusted with lime last night. My cactus soil PH is ~6.2, hopefully I'll have raised this batch closer to 8 and will be able to see for myself is I get casing colonization with this same culture.
I ran this culture with and without casing and it produced way better pinset and fruits than without. But other members never needed to case and had stellar results too.
Here is a side by side of non-cased vs cased, same LC, same innoc date etc.:

yes, buddy, it's your lineage, thank you again <3 So it's all about acidity...When you say "hopefully I'll have raised this batch closer to 8ph" I infer you didn't get a measuring device? Did you follow a standard ratio?
Guess I'll have to buy some limestone and take it further for PE culture too
thanks all
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Melgo
Semperviva Fanatic



Registered: 02/11/16
Posts: 692
Loc: Western Europe
Last seen: 8 minutes, 50 seconds
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Gleyck] 2
#28612385 - 01/06/24 09:56 PM (22 days, 1 hour ago) |
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Quote:
Gleyck said: yes, buddy, it's your lineage, thank you again <3 So it's all about acidity...When you say "hopefully I'll have raised this batch closer to 8ph" I infer you didn't get a measuring device? Did you follow a standard ratio?
Guess I'll have to buy some limestone and take it further for PE culture too
thanks all
I followed faht:Detailed Peat/Verm Casing Prep Instructions ratios (so one tsp of lime per cup of cactus soil) and used PH strips to measure. Might need to use more next time, it's my first try so ya know
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chris77
Archaic Revivalist



Registered: 01/17/22
Posts: 1,868
Last seen: 6 hours, 48 minutes
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Melgo] 2
#28612481 - 01/07/24 12:48 AM (21 days, 22 hours ago) |
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Personally i am using between 5 and 10 % limestone in a properly pasteurized peat/verm casing. Does the trick for me. after that it really depends on when i apply the casing (half inch approx) Also i am under the impression that it tends to be overrun by mycelium when the casing is not wet enough. Just my 2c
-------------------- the observer is the observed j. krishnamurti
Edited by chris77 (01/07/24 10:21 AM)
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RoscoeReturns
Crotchety chode man



Registered: 02/12/18
Posts: 1,738
Loc: State of Confusion
Last seen: 6 hours, 53 minutes
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: chris77] 6
#28612662 - 01/07/24 06:59 AM (21 days, 16 hours ago) |
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All this talk of casing and I ran out. I guess we’ll see which works better.
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