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Invisiblethe_chosen_one
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Re: Official Hoogshagenii/Semperviva Thread [Re: multifractal] * 1
    #27476238 - 09/20/21 08:40 PM (2 years, 4 months ago)

Looks like I'll be jumping in on the fun eventually. It's been years since I've grown this one. I'm excited to hurry up and wait. :lol:
Pearl Barley. Multi spore injection on 9/6. The right one was shaken fairly early for the heck of it. The left not at all short of right after injection.


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"Luck favors the observant." - Workman



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InvisibleAmbrose

Registered: 01/16/20
Posts: 518
Re: Official Hoogshagenii/Semperviva Thread [Re: the_chosen_one] * 2
    #27479697 - 09/23/21 04:15 PM (2 years, 4 months ago)

TCO! It's awesome to have you join us :bow2:



Finally getting some pins after a string of failures(trays just refused to pin for shite.) Fingers crossed they mature okay.

These are the fastest I've had to pin so far, 4 weeks instead of 6(still need to reduce that time as I know y'all get shorter times.) Main difference was that the jar it came from sat around for like 4 months. Is that just a coincidence or has anyone else noticed faster fruiting from spawn that's been consolidating for awhile?





Edit:
:facepalm: should have read the previous page
Quote:

alaskappalachian said:
Yeah, actually I noticed a fairly sizable difference in fruiting time with my super-consolidated subtropicalis spawn.  Like almost two weeks.  Could be the cultures I've run, but seems like a good tradeoff if so.  Running some now that I plan to consolidate for a while,  actually...






Edited by Ambrose (09/23/21 08:18 PM)


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OfflineA.k.aM
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Re: Official Hoogshagenii/Semperviva Thread [Re: Ambrose]
    #27479767 - 09/23/21 05:37 PM (2 years, 4 months ago)

Wow those are awesome clusters.



So it sounds like these have a long window before they hit maturity regardless of whether they’re in a jar or spawned.


I just got germ on my spore plate to take another shot at these. Guess I’ll probably be letting my spawn sit a couple weeks.


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OfflineAyEyDiZed
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Re: Official Hoogshagenii/Semperviva Thread [Re: A.k.a]
    #27483166 - 09/26/21 08:13 PM (2 years, 3 months ago)

I’m jumping in!

Been meaning to start on this for awhile but focused on other stuff until now.

I failed first time. Both agar plate and MS brf jar contaminated bad. Not sure if I wasn’t sterile enough or didnt pc my jars enough. But alas I tried again and have great looking growth.

I have one brf cake that’s pinning invitro. I’m in two minds about birthing and fruiting the cake in a sgfc or spawn to bulk and monotub it.

I’ll upload some pics after I cull my old ones. (I had the free sponsorship but when it expired I was way over the allowed uploads and need to drastically cull them). Or pay for sponsorship lol.


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Invisiblemultifractal
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Re: Official Hoogshagenii/Semperviva Thread [Re: AyEyDiZed]
    #27485612 - 09/28/21 07:21 PM (2 years, 3 months ago)

So i have a 32 qt tub that I cased about 10 days ago:



As you can see the mycelium is beginning to emerge from beneath the casing, which was coir on the drier side. None of my other tubs are exhibiting this behavior so I'm concerned I might have made the casing either too thin or too wet.

Do you guys think I should re-case after the existing top layer is completely colonized, which it seems will be the case, add more casing now, or should I scrape the existing one off and try again?

I really appreciate your help.


Edited by multifractal (09/28/21 09:03 PM)


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Invisiblenosf3r4tu


Registered: 03/26/19
Posts: 775
Reply to Official Ps. Subtropicalis/Semperviva Thread [Re: nosf3r4tu]
    #27485801 - 09/28/21 10:16 PM (2 years, 3 months ago)

You could scrape it and add a proper casing layer. Like peat or verm.


Edited by nosf3r4tu (09/28/21 10:18 PM)


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InvisibleAmbrose

Registered: 01/16/20
Posts: 518
Re: Reply to Official Ps. Subtropicalis/Semperviva Thread [Re: nosf3r4tu] * 6
    #27490133 - 10/02/21 04:39 PM (2 years, 3 months ago)

Here's that tray I posted previously. Guess maybe I should not have thrown out the culture it came from, oops :lol:.
Pleasantly surprised to see what I think is some sporulation goin on, not sure when I should attempt to print, looks like a couple caps are maybe ready?


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OfflineA.k.aM
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Re: Reply to Official Ps. Subtropicalis/Semperviva Thread [Re: Ambrose]
    #27490164 - 10/02/21 05:10 PM (2 years, 3 months ago)

Daaaaaamn


--------------------
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InvisibleWall.E
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Re: Reply to Official Ps. Subtropicalis/Semperviva Thread [Re: Ambrose]
    #27490242 - 10/02/21 06:22 PM (2 years, 3 months ago)

Quote:

Ambrose said:
Here's that tray I posted previously. Guess maybe I should not have thrown out the culture it came from, oops :lol:.
Pleasantly surprised to see what I think is some sporulation goin on, not sure when I should attempt to print, looks like a couple caps are maybe ready?





Killing it as always my man


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Life’s shit, but I’m loving it



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InvisibleAmbrose

Registered: 01/16/20
Posts: 518
Re: Reply to Official Ps. Subtropicalis/Semperviva Thread [Re: Wall.E]
    #27490275 - 10/02/21 06:48 PM (2 years, 3 months ago)

Thanks y'all. Can't seem to figure these guys out, they just continue to surprise me. Gotta find the time to try eating some too :mushroom2:


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Invisiblethe_chosen_one
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Re: Reply to Official Ps. Subtropicalis/Semperviva Thread [Re: Ambrose]
    #27490280 - 10/02/21 06:57 PM (2 years, 3 months ago)

One of the best canopies I've seen! Really nice work Ambrose. The caps with up turned edges are very likely dropping. Hard to see the little shits isn't it lol. Normally you can see them on the stem base. Doubt they can get there right now. :lol:


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"Luck favors the observant." - Workman



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InvisibleAmbrose

Registered: 01/16/20
Posts: 518
Re: Reply to Official Ps. Subtropicalis/Semperviva Thread [Re: the_chosen_one]
    #27490295 - 10/02/21 07:24 PM (2 years, 3 months ago)

:heart: Thank you TCO! :bow2:


Yeah they are difficult to see. Lil fellas sometimes seem stingy with the spores.  Will try to print a handful of caps and see what happens.
:prayer:


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OfflineAsuraS
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Re: Reply to Official Ps. Subtropicalis/Semperviva Thread [Re: Ambrose]
    #27490321 - 10/02/21 07:54 PM (2 years, 3 months ago)

Quote:

Ambrose said:
Here's that tray I posted previously. Guess maybe I should not have thrown out the culture it came from, oops :lol:.
Pleasantly surprised to see what I think is some sporulation goin on, not sure when I should attempt to print, looks like a couple caps are maybe ready?





Ambrose, this is super-motivating. I have 8 different cultures going right now. I haven't ever dosed these, but a close friend
who is also really experienced with pan cyans told me it was the best trip she's ever had. The trip report was epic.
Anyway, way to kill it.


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InvisibleAmbrose

Registered: 01/16/20
Posts: 518
Re: Reply to Official Ps. Subtropicalis/Semperviva Thread [Re: Asura] * 1
    #27490352 - 10/02/21 08:20 PM (2 years, 3 months ago)

Thank you! Glad to hear that :heart:

And wow, now I'm really excited to try dosing these, keep seeing positive posts about the trip :lol:

It seems like getting a good culture is important. I may have already mentioned it but this was one of a handful of clones. Each one I spawned performed so poorly I basically gave up trying to grow the species. Then 4 months later found this one I hadn't spawned and decided fuck it why not?
Will have to take a clone from this tray to get it back on agar, not even sure if I'll be able to get good results again from them. Just gotta try again and see what happens I suppose.
:rockon:


Edited by Ambrose (10/02/21 08:41 PM)


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Invisiblethe_chosen_one
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Re: Reply to Official Ps. Subtropicalis/Semperviva Thread [Re: Ambrose] * 1
    #27490738 - 10/03/21 08:00 AM (2 years, 3 months ago)

Yeah, the trip is unique compared to other species. Potency can vary from crop to crop. I'm suspicious that what they are growing on plays a role. When fruited on millet I've had batches where .4 = stuck on the floor. :lol:
I've had batches grown on other substrates where 1.4 = I could handle more. :shrug:
Physical condition as well as set and setting as with most actives is a factor, but the millet thing has happened enough times to make me feel there's something to it. Plus RR posted an article a long time ago in regards to millet grown Cordyceps producing higher levels of desired compounds. This may have influenced my Set.. but I tend to believe the science.

So I spawned the shaken jar to straw a few days ago where they are moving slower than molasses in January. Zapos done at the same time are walking all over them.
I think I'll let the unshaken jar consolidate some more before I decide what to do with it. It's tempting to just case and fruit from the jar. If the straw trays make it to fruition it might make a good comparison. Even if it is pearl barely. Maybe a millet run is in order next.


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OfflineA.k.aM
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Re: Reply to Official Ps. Subtropicalis/Semperviva Thread [Re: the_chosen_one]
    #27490746 - 10/03/21 08:13 AM (2 years, 3 months ago)

That makes me super excited hearing about the trip.


I half assed one tub of these a while back and nothing happened. I’ve got a clean germ plate with four of five solid colonies on it ready for transfers.


The millet thing is awesome too cuz I’m all wbs/millet.


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InvisibleGuerrilla
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Re: Reply to Official Ps. Subtropicalis/Semperviva Thread [Re: the_chosen_one]
    #27490749 - 10/03/21 08:14 AM (2 years, 3 months ago)

Quote:

the_chosen_one said:
Yeah, the trip is unique compared to other species. Potency can vary from crop to crop. I'm suspicious that what they are growing on plays a role. When fruited on millet I've had batches where .4 = stuck on the floor. :lol:
I've had batches grown on other substrates where 1.4 = I could handle more. :shrug:
Physical condition as well as set and setting as with most actives is a factor, but the millet thing has happened enough times to make me feel there's something to it. Plus RR posted an article a long time ago in regards to millet grown Cordyceps producing higher levels of desired compounds. This may have influenced my Set.. but I tend to believe the science.

So I spawned the shaken jar to straw a few days ago where they are moving slower than molasses in January. Zapos done at the same time are walking all over them.
I think I'll let the unshaken jar consolidate some more before I decide what to do with it. It's tempting to just case and fruit from the jar. If the straw trays make it to fruition it might make a good comparison. Even if it is pearl barely. Maybe a millet run is in order next.




This makes me wanna buy millet and I’ve only ever been a rye man..


--------------------
Being pissed on does not make you a real man.


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Invisiblethe_chosen_one
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Re: Reply to Official Ps. Subtropicalis/Semperviva Thread [Re: Guerrilla]
    #27490758 - 10/03/21 08:23 AM (2 years, 3 months ago)

It's an interesting and initially challenging grain to work with. It will definitely add to your skills.
:awesomenod:

To add more to my suspicions I've even had friends comment on the potency without knowing what they were grown on.


--------------------
"Luck favors the observant." - Workman



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InvisibleRumblestrip


Registered: 04/21/19
Posts: 258
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Re: Reply to Official Ps. Subtropicalis/Semperviva Thread [Re: the_chosen_one]
    #27490938 - 10/03/21 11:31 AM (2 years, 3 months ago)

Grain nutrients affecting potency seems to make sense. I noticed differences in growth rate, sclerotia formation and potency when putting mexicana A on either white or brown rice.


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OfflineAsuraS
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Re: Reply to Official Ps. Subtropicalis/Semperviva Thread [Re: Rumblestrip]
    #27491059 - 10/03/21 01:12 PM (2 years, 3 months ago)

TCO, sorry if I missed it, but what's your substrate? My last round was straight coir, cased with 50/50.
Pulled a whole 6g dry :lol:

Was going to do coffee supplemented coir next round based on some other grows, but I gotta say the
doctor says coffee makes me nervous.


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