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Offlinechris77
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Melgo] * 2
    #28394049 - 07/13/23 02:38 AM (6 months, 13 days ago)

I love how this thread develops and the mystery around the lineages, are they slow, fast, or simply depending on factors that we don't know about just yet? Very intriguing.

@Melgo same here, planning to trip on semperviva in the near future. i'll be interested to hear from your experience regarding dosage etc.
:aweyeah:


--------------------
the observer is the observed
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OfflineWONKA
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Re: Official Ps. Subtropical/Semperviva Thread [Re: chris77] * 2
    #28394519 - 07/13/23 03:12 PM (6 months, 13 days ago)

If you look back at sirpsycho grow with the mushrooms growing with grass in the pots that these mushrooms show full genetic expression. I have a hypothesis that this has to due with a symbiotic relationship with certain species of grass that produce Endophytes.I have suspicion that subtropicalis, mexicana, and semilanceata have a symbiotic relationship with these Endophytes. https://en.m.wikipedia.org/wiki/Endophyte Here is a amazon link to some  endophytic turfgrass varieties. https://www.amazon.com/gp/aw/d/B002JGWYUM?ref=ppx_pt2_mob_b_prod_image


Edited by WONKA (07/13/23 03:21 PM)


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OfflineSirPsycho
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Re: Official Ps. Subtropical/Semperviva Thread [Re: WONKA] * 3
    #28394583 - 07/13/23 04:41 PM (6 months, 13 days ago)

No grass, last year


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
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OfflinePsilosophy328
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Re: Official Ps. Subtropical/Semperviva Thread [Re: WONKA]
    #28394675 - 07/13/23 07:10 PM (6 months, 13 days ago)

Quote:

WONKA said:
If you look back at sirpsycho grow with the mushrooms growing with grass in the pots that these mushrooms show full genetic expression. I have a hypothesis that this has to due with a symbiotic relationship with certain species of grass that produce Endophytes.I have suspicion that subtropicalis, mexicana, and semilanceata have a symbiotic relationship with these Endophytes. https://en.m.wikipedia.org/wiki/Endophyte Here is a amazon link to some  endophytic turfgrass varieties. https://www.amazon.com/gp/aw/d/B002JGWYUM?ref=ppx_pt2_mob_b_prod_image




Dude I've been thinking the same thing! Except in the context of  Panaeolus cinctulus/Panaeolus fimicola. They grow in fields near me that are well kept and are just starting to come out now.

But the other day I was thinking "huh I bet these mushrooms are mineralizing the phosphorus in the fertilizer and exchanging it for sugar with the grass" and that's why you often find P. cinctulus and other similar species in well cared for lawns. They help solubilize (prob not a word) the fertilizer and get sugar in return from the grass.

It's crazy the level of communication and interaction that occurs, in ways very similar to our lives, but between fungi and plants. And we're just scratching the surface. Should be an interesting ride.


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OfflinePsilosophy328
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Re: Official Ps. Subtropical/Semperviva Thread [Re: SirPsycho]
    #28394678 - 07/13/23 07:12 PM (6 months, 13 days ago)

Quote:

SirPsycho said:
No grass, last year





Super interesting! I'm interested to see more.

Did you do anything with the grass seeds to avoid contamination in the casing?


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OfflineSirPsycho
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Re: Official Ps. Subtropical/Semperviva Thread [Re: Psilosophy328] * 1
    #28394703 - 07/13/23 07:40 PM (6 months, 13 days ago)

Quote:

Psilosophy328 said:
Quote:

SirPsycho said:
No grass, last year





Super interesting! I'm interested to see more.

Did you do anything with the grass seeds to avoid contamination in the casing?



No, and I did get quite a few moldy seeds (those that didn't germinate) when burying cakes whole. The grass itself in the subtrop pot seemed to be moldy but honestly it could have been subtrop myc.

The tampanesis pots I got going, the cakes were broken up to harvest stones and the myc seems to have colonized into the casing so I doubt those will mold.

I made another pot of subtropicalis with no grass recently and I just inoculated more jars with subtropicalis today. I also made a pan cyan pot with no grass at the same time as the subtrop and I did a pot of nats and cubes each, those got clover seed.


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  πŸ…‘πŸ…žπŸ…£πŸ…£πŸ…›πŸ…” πŸ…–πŸ…πŸ…πŸ…–

"Mist your balls and fan your asshole" - Pandaskis, 2023


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Offlinerockyfungus
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Re: Official Ps. Subtropical/Semperviva Thread [Re: SirPsycho] * 2
    #28395087 - 07/14/23 07:43 AM (6 months, 12 days ago)

Not going to act like i'm an endophyte expert. But endophytes are in seeds not our spores. Also found in actual grass and plant material.

So more like a host then some symbiosis. We're also not talking about myccorhz mush.


Edited by rockyfungus (07/14/23 07:56 AM)


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Invisiblemilkboy
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Re: Official Ps. Subtropical/Semperviva Thread [Re: rockyfungus] * 15
    #28395247 - 07/14/23 10:31 AM (6 months, 12 days ago)

First crack at semps, thanks faht! I am now a simp for semps :awesomenod:

1 pint spawn then


Now

Just waitin, IM WAITING:camping:


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OfflineWONKA
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Re: Official Ps. Subtropical/Semperviva Thread [Re: rockyfungus]
    #28395249 - 07/14/23 10:35 AM (6 months, 12 days ago)

Much like the guts of humans like you and me, plants harbor a variety of fungi and bacteria within their tissues. Fungi, bacteria, and other microorganisms in plants–called endophytes–have been found to improve plant health, both under normal and challenging environmental conditions. I guess the idea iam playing with in my head is that the endophyte may help protect the mycelium from pathogens by offering immunsupport from competitive molds and bacteria. Plus the areobic conditions produced by the root ryzomes would be favorable for mushroom species that live below the soil line,unlike cubes that live above the soil line and exist in a toltaly different microbiom type environment.


Edited by WONKA (07/14/23 10:42 AM)


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InvisibleKonbri
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Re: Official Ps. Subtropical/Semperviva Thread [Re: milkboy] * 2
    #28395455 - 07/14/23 02:03 PM (6 months, 12 days ago)

Quote:

milkboy said:
First crack at semps, thanks faht! I am now a simp for semps :awesomenod:

1 pint spawn then


Now

Just waitin, IM WAITING:camping:




Awesome!!!
Congrats!


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Offlinechris77
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Re: Official Ps. Subtropical/Semperviva Thread [Re: Konbri] * 1
    #28395464 - 07/14/23 02:16 PM (6 months, 12 days ago)

Quote:

Konbri said:
Quote:

milkboy said:
First crack at semps, thanks faht! I am now a simp for semps :awesomenod:

1 pint spawn then


Now

Just waitin, IM WAITING:camping:




Awesome!!!
Congrats!



:whathesaid::aweyeah:


--------------------
the observer is the observed
            j. krishnamurti


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OfflineSirPsycho
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Re: Official Ps. Subtropical/Semperviva Thread [Re: chris77] * 15
    #28395598 - 07/14/23 04:46 PM (6 months, 12 days ago)

23 days since first signs of pins



--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  πŸ…‘πŸ…žπŸ…£πŸ…£πŸ…›πŸ…” πŸ…–πŸ…πŸ…πŸ…–

"Mist your balls and fan your asshole" - Pandaskis, 2023


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Invisiblemilkboy
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Re: Official Ps. Subtropical/Semperviva Thread [Re: SirPsycho] * 2
    #28395611 - 07/14/23 05:05 PM (6 months, 12 days ago)

Those aren't ready yet


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OfflineSirPsycho
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Re: Official Ps. Subtropical/Semperviva Thread [Re: milkboy] * 2
    #28395616 - 07/14/23 05:08 PM (6 months, 12 days ago)

No, they are not


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  πŸ…‘πŸ…žπŸ…£πŸ…£πŸ…›πŸ…” πŸ…–πŸ…πŸ…πŸ…–

"Mist your balls and fan your asshole" - Pandaskis, 2023


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OfflineThe Tao
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Re: Official Ps. Subtropical/Semperviva Thread [Re: SirPsycho] * 2
    #28395627 - 07/14/23 05:23 PM (6 months, 12 days ago)

Handsome caps. Those are nice.


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OfflinePsilosophy328
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Re: Official Ps. Subtropical/Semperviva Thread [Re: rockyfungus]
    #28395757 - 07/14/23 07:38 PM (6 months, 12 days ago)

Quote:

rockyfungus said:
Not going to act like i'm an endophyte expert. But endophytes are in seeds not our spores. Also found in actual grass and plant material.

So more like a host then some symbiosis. We're also not talking about myccorhz mush.




I wouldnt be surprised if it's unknown whether P fimicola has an endophytic relationship with different grass species.


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OfflineRoscoeReturnsS
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Re: Official Ps. Subtropical/Semperviva Thread [Re: The Tao] * 13
    #28395787 - 07/14/23 08:10 PM (6 months, 12 days ago)

I’m beginning to believe Melgo may have the right of it, and conditions are the real defining factor in the differences we are seeing. I got lucky with my MS grow of spores of Melgo’s fast fruiting culture. I wouldn’t consider them fast IME, but I got a nice flush my first try. I cloned one of the fruit from my first grow, cleaned it up on agar, and made an LC. All of my grows since have been from that LC. All have been different. I’m still throwing everything at these, just to see what happens. Here are a few observations I’ve made.

My first few bags and bottles from the LC were grown in Winter. Conditions were very stable. Temp around 70F and steady, RH of the room around 30%. This didn’t change day to night, or throughout the grow as my heat was on all the time. It gets cold here, and dark, only about 9 hours of daylight. Fruited the bags in an EZ dial. I used different substrates and different casing layer, but all performed about the same. Nice fruit 5” tall, caps 3/4” to 1 1/2” across. Pretty even dense pinset. Like these:



My next few bags from the same LC, same substrate as the pictured bag, but due to a house guest, and change of season the growing conditions were vastly different. These were also in an EZ dial mono, but in a different room. Heat was off as outside temps were on the rise. Room temps varied greatly day to night low 60’s up in to the 80’s. RH in the room also varied 30% to 60%. Daylight for about twelve hours. These were slower, about 10 days longer to spore drop. They also grew much bigger, thicker stipes, 6” tall, caps 2” across or more. Pin sets were not that great, but I got some monsters like SirP has in his flower pot.



Now it’s down right hot here. I still have a few bags in an EZ dial, temps 70 - 90F, RH in the room 40%-70%, much longer days 15 hours of light. Fruits now are growing slow again, pin set is again sporadic. Stipes are not thick, caps are small, many are aborting. Too hot? Heat drying out the substrate?

I also threw a bag in the tent I use to grow gourmets. Totally different conditions, same culture, different results. The RH in the tent is always above 90%, but temps are steady 65F. The tent is in my basement, and gets much less light than any of my prior grows. 12 hours on, but much less intense and no sunlight all artificial. Fog and fresh air fan run three times an hour. The casing is a bit on the dry side. The fruits are super slow, but thicc! Chubby little guys that grow really slow, fat stipes, small caps, not sure how long to spore because they haven’t got there yet.



All from the same clone. All supposedly the fast variety, but I’ve not had pins any sooner than 3 weeks from casing. I’m thinking I have no idea what these guys really prefer, nor what they are β€œsupposed” to look like. I do know I love these guys, and the experience when I eat them. I’ll be going back to spore for my next run, and trying a few different things as well as repeating my last few conditions with the new culture. For those who have grown these often, do clones perform better than MS grows? I still consider my first few bags my best results.


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InvisibleMojaveMyc
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Re: Official Ps. Subtropical/Semperviva Thread [Re: RoscoeReturns] * 8
    #28396075 - 07/15/23 07:38 AM (6 months, 11 days ago)

Great observations, Roscoe. Particularly about temperature, I'll have to keep an eye on mine next time around. As far as clone vs. MS goes, my best performers were MS & clones have been hit or miss, mostly misses.

MS:


Clones:


--------------------


Edited by MojaveMyc (07/15/23 09:07 AM)


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Invisiblekirkeng
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Re: Official Ps. Subtropical/Semperviva Thread [Re: MojaveMyc] * 8
    #28396104 - 07/15/23 08:08 AM (6 months, 11 days ago)

I agree, more observations like these can help us crack the mystery! I’ve had slightly better results from clones personally, not majorly but noticeable. MS still performs excellently though. Comparative density:
MS:


Clone:


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OfflineJW123
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: kirkeng] * 4
    #28396501 - 07/15/23 01:27 PM (6 months, 11 days ago)

Feels like the first time again.

A month and a half since S2B 1:3 spawn to coir with a half quart of coir pseudo casing at spawn. One of my shoeboxes has molded, one looks like it is about to, and this is the third one. It has three clearly visible pins. Thank you again 0t0lerance. I am very excited.


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